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The map label for this gene is mltD [H]

Identifier: 237811765

GI number: 237811765

Start: 1438717

End: 1440285

Strand: Direct

Name: mltD [H]

Synonym: GBP346_A1503

Alternate gene names: 237811765

Gene position: 1438717-1440285 (Clockwise)

Preceding gene: 237811764

Following gene: 237811766

Centisome position: 35.1

GC content: 67.88

Gene sequence:

>1569_bases
TTGGTCCTGACGCTCGCCGCGTGCGCGGGCACCGGGCCGACCGCCCAAAATCCCGCAGCCTCCGACCCGCAAGCCGCTTC
GGACTACTTGCGCAAGTCCGCTTCCGCGAAAGAAACCGTCGACGTCGACAAGCAGTCGGTCGGAGACCTCACCACCGCCG
ACAGCGATCTCTGGGCGCGCATCCGCCGCGGCTTCCAGATGCCCGACCTGCAGAGCGATCTCGTCGACATGCAGGCGTCG
TGGTACGCGCAGCGCCCCGACTATGTGCAGCGGATGACCGAACGCTCGCAGAAGTACCTGTACCACATCGTCGAGGAGCT
CGAATCGCGCCACATGCCGACCGAACTCGCGCTGCTGCCGTTCATCGAATCGGCGTACAACCCGCAGGCGCTGTCCGTCG
CGAAGGCGGCGGGGATGTGGCAGTTCATGCCGGCTACGGGGCGCACGTTCAACCTGAAGCGCAACATGTGGCAGGACGAG
CGGCGCGACGTGCTCGCGTCGACGAGCGCGGCGCTCGATTACCTGTCGCGCCTGCATGACATGTTCGGCGACTGGTATCT
CGCGCTCGCCGCGTACAACTGGGGCGAGGGCAATCTGCAGCGCGCGATCGCGCGCAACCAGGCGGCGGGGCTGCCGACCG
ATTACCAGAGCCTGCGGATGCCGAACGAGACGCGCAACTACGTGCCGAAGCTGCAGGCGGTCAAGAACATCGTGATGAAC
CCGCAGCAGTACGGCCTCACGCTGCCGGAGATTCCGAACCACCCGTATTTCGTGACGGTCACGACCTCGCGCGACATCGA
CGTGACGGTCGCCGCGAAGCTCGCGAACCTGCCGCTCGACGAATTCAAGTCGCTCAACCCGTCGTTCTCGAAGCCCGTGA
TTCTCGGTGCGACGCAGCCGCAGATCCTGCTGCCGTTCGATAACGCGGCGGCGTTCGAGAAGGGGCTGAAGTCGTACGAC
GGCCAGCTTTCGTCATGGACCGCGTATACGGTCACCGAGCGCGCGCGGCCGGCCGCGATCGCGGAGAAGATCGGCGTCGA
CGCGGATACGCTGATGCAGGTGAACAAGATTCCGGCCGGCATGCGGCTGAAGCCCGGCTCGACGATCGTCGTGCCGCGCA
CCGACGACGATGACGAGGACATCAGCGCCGACGTCGCCGAGAACGGCGTGCTCGCGATGGAGCCGGACGTGCCCGACACG
CGCAAGATGTTGATTCGCGTGCGCCGCAAGCAGTCGATGGAAGCGCTCGCGAGCCGCTACGGCGTGTCGGTCGCGCAACT
GCGCGGATGGAACCGCACGCGCCGCGCGGTCGTGATGCCGGGCCAGACGATGGTGCTGCACGTGCCCGTCGGTCGCGCGG
TGCCGGCCGAGCCGGGGCCCGAGCGGATCGCGACGTCGGTAGGCGGCGGGCGCATCGAGCGCGCGAGCCTGCACACGGGC
GGCAAGGCGTCGAGCCGCGGCCGCAAGGCGGCGCCCGCCGCGAAGGCGTCGGCGAAGGCCGCGACGAAGAGCGGTGCGAA
AGCCGCGCCCGCGAAAACCGCGGGCCGCAAGACGACGAAGAAGAAGTAA

Upstream 100 bases:

>100_bases
GGCCTTTTTGTTGACGCGAAGCACGCACTTTCGTACTATCGCCTGCAATTTCCAGCCGTTGGAAGCCGAGATTTTCATGC
GATTCCTCCTCAGTGCGCTA

Downstream 100 bases:

>100_bases
GACGAAAGGGCGCGCATGCCGCGGGGGCTTTCCTGCCGGCGACGCGCGATCGACTATCATCGCCGGACGCCGTCACCTGG
GTGACGGCGTTTTCGTTTGC

Product: lytic transglycosylase catalytic

Products: 1,6-Anhydrobond In The Muramic Acid Residue [C]

Alternate protein names: Murein hydrolase D; Regulatory protein dniR [H]

Number of amino acids: Translated: 522; Mature: 522

Protein sequence:

>522_residues
MVLTLAACAGTGPTAQNPAASDPQAASDYLRKSASAKETVDVDKQSVGDLTTADSDLWARIRRGFQMPDLQSDLVDMQAS
WYAQRPDYVQRMTERSQKYLYHIVEELESRHMPTELALLPFIESAYNPQALSVAKAAGMWQFMPATGRTFNLKRNMWQDE
RRDVLASTSAALDYLSRLHDMFGDWYLALAAYNWGEGNLQRAIARNQAAGLPTDYQSLRMPNETRNYVPKLQAVKNIVMN
PQQYGLTLPEIPNHPYFVTVTTSRDIDVTVAAKLANLPLDEFKSLNPSFSKPVILGATQPQILLPFDNAAAFEKGLKSYD
GQLSSWTAYTVTERARPAAIAEKIGVDADTLMQVNKIPAGMRLKPGSTIVVPRTDDDDEDISADVAENGVLAMEPDVPDT
RKMLIRVRRKQSMEALASRYGVSVAQLRGWNRTRRAVVMPGQTMVLHVPVGRAVPAEPGPERIATSVGGGRIERASLHTG
GKASSRGRKAAPAAKASAKAATKSGAKAAPAKTAGRKTTKKK

Sequences:

>Translated_522_residues
MVLTLAACAGTGPTAQNPAASDPQAASDYLRKSASAKETVDVDKQSVGDLTTADSDLWARIRRGFQMPDLQSDLVDMQAS
WYAQRPDYVQRMTERSQKYLYHIVEELESRHMPTELALLPFIESAYNPQALSVAKAAGMWQFMPATGRTFNLKRNMWQDE
RRDVLASTSAALDYLSRLHDMFGDWYLALAAYNWGEGNLQRAIARNQAAGLPTDYQSLRMPNETRNYVPKLQAVKNIVMN
PQQYGLTLPEIPNHPYFVTVTTSRDIDVTVAAKLANLPLDEFKSLNPSFSKPVILGATQPQILLPFDNAAAFEKGLKSYD
GQLSSWTAYTVTERARPAAIAEKIGVDADTLMQVNKIPAGMRLKPGSTIVVPRTDDDDEDISADVAENGVLAMEPDVPDT
RKMLIRVRRKQSMEALASRYGVSVAQLRGWNRTRRAVVMPGQTMVLHVPVGRAVPAEPGPERIATSVGGGRIERASLHTG
GKASSRGRKAAPAAKASAKAATKSGAKAAPAKTAGRKTTKKK
>Mature_522_residues
MVLTLAACAGTGPTAQNPAASDPQAASDYLRKSASAKETVDVDKQSVGDLTTADSDLWARIRRGFQMPDLQSDLVDMQAS
WYAQRPDYVQRMTERSQKYLYHIVEELESRHMPTELALLPFIESAYNPQALSVAKAAGMWQFMPATGRTFNLKRNMWQDE
RRDVLASTSAALDYLSRLHDMFGDWYLALAAYNWGEGNLQRAIARNQAAGLPTDYQSLRMPNETRNYVPKLQAVKNIVMN
PQQYGLTLPEIPNHPYFVTVTTSRDIDVTVAAKLANLPLDEFKSLNPSFSKPVILGATQPQILLPFDNAAAFEKGLKSYD
GQLSSWTAYTVTERARPAAIAEKIGVDADTLMQVNKIPAGMRLKPGSTIVVPRTDDDDEDISADVAENGVLAMEPDVPDT
RKMLIRVRRKQSMEALASRYGVSVAQLRGWNRTRRAVVMPGQTMVLHVPVGRAVPAEPGPERIATSVGGGRIERASLHTG
GKASSRGRKAAPAAKASAKAATKSGAKAAPAKTAGRKTTKKK

Specific function: Murein-degrading enzyme. May play a role in recycling of muropeptides during cell elongation and/or cell division [H]

COG id: COG0741

COG function: function code M; Soluble lytic murein transglycosylase and related regulatory proteins (some contain LysM/invasin domains)

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 LysM repeats [H]

Homologues:

Organism=Escherichia coli, GI1786405, Length=459, Percent_Identity=32.6797385620915, Blast_Score=231, Evalue=7e-62,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008258
- InterPro:   IPR010511
- InterPro:   IPR018392
- InterPro:   IPR002482
- InterPro:   IPR000189 [H]

Pfam domain/function: PF01476 LysM; PF06474 MLTD_N; PF01464 SLT [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 57086; Mature: 57086

Theoretical pI: Translated: 9.99; Mature: 9.99

Prosite motif: PS00922 TRANSGLYCOSYLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVLTLAACAGTGPTAQNPAASDPQAASDYLRKSASAKETVDVDKQSVGDLTTADSDLWAR
CEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHCCCCHHHHCCCHHHCCCCCCCCHHHHHH
IRRGFQMPDLQSDLVDMQASWYAQRPDYVQRMTERSQKYLYHIVEELESRHMPTELALLP
HHCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
FIESAYNPQALSVAKAAGMWQFMPATGRTFNLKRNMWQDERRDVLASTSAALDYLSRLHD
HHHHCCCCHHHHHHHHCCCEEEECCCCCEEECHHHCCHHHHHHHHHHHHHHHHHHHHHHH
MFGDWYLALAAYNWGEGNLQRAIARNQAAGLPTDYQSLRMPNETRNYVPKLQAVKNIVMN
HHHHHEEHHEECCCCCHHHHHHHHHHHCCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHCC
PQQYGLTLPEIPNHPYFVTVTTSRDIDVTVAAKLANLPLDEFKSLNPSFSKPVILGATQP
CHHHCCCCCCCCCCCEEEEEECCCCCEEEEEHHHHCCCHHHHHCCCCCCCCCEEEECCCC
QILLPFDNAAAFEKGLKSYDGQLSSWTAYTVTERARPAAIAEKIGVDADTLMQVNKIPAG
EEEEECCCHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHCCCHHHHHHHHCCCCC
MRLKPGSTIVVPRTDDDDEDISADVAENGVLAMEPDVPDTRKMLIRVRRKQSMEALASRY
CEECCCCEEEEECCCCCCCCCHHHHHCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHH
GVSVAQLRGWNRTRRAVVMPGQTMVLHVPVGRAVPAEPGPERIATSVGGGRIERASLHTG
CCHHHHHCCCCCCCEEEEECCCEEEEEECCCCCCCCCCCHHHHHHHCCCCCEEEEECCCC
GKASSRGRKAAPAAKASAKAATKSGAKAAPAKTAGRKTTKKK
CCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCHHCCCCCCCCC
>Mature Secondary Structure
MVLTLAACAGTGPTAQNPAASDPQAASDYLRKSASAKETVDVDKQSVGDLTTADSDLWAR
CEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHCCCCHHHHCCCHHHCCCCCCCCHHHHHH
IRRGFQMPDLQSDLVDMQASWYAQRPDYVQRMTERSQKYLYHIVEELESRHMPTELALLP
HHCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
FIESAYNPQALSVAKAAGMWQFMPATGRTFNLKRNMWQDERRDVLASTSAALDYLSRLHD
HHHHCCCCHHHHHHHHCCCEEEECCCCCEEECHHHCCHHHHHHHHHHHHHHHHHHHHHHH
MFGDWYLALAAYNWGEGNLQRAIARNQAAGLPTDYQSLRMPNETRNYVPKLQAVKNIVMN
HHHHHEEHHEECCCCCHHHHHHHHHHHCCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHCC
PQQYGLTLPEIPNHPYFVTVTTSRDIDVTVAAKLANLPLDEFKSLNPSFSKPVILGATQP
CHHHCCCCCCCCCCCEEEEEECCCCCEEEEEHHHHCCCHHHHHCCCCCCCCCEEEECCCC
QILLPFDNAAAFEKGLKSYDGQLSSWTAYTVTERARPAAIAEKIGVDADTLMQVNKIPAG
EEEEECCCHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHCCCHHHHHHHHCCCCC
MRLKPGSTIVVPRTDDDDEDISADVAENGVLAMEPDVPDTRKMLIRVRRKQSMEALASRY
CEECCCCEEEEECCCCCCCCCHHHHHCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHH
GVSVAQLRGWNRTRRAVVMPGQTMVLHVPVGRAVPAEPGPERIATSVGGGRIERASLHTG
CCHHHHHCCCCCCCEEEEECCCEEEEEECCCCCCCCCCCHHHHHHHCCCCCEEEEECCCC
GKASSRGRKAAPAAKASAKAATKSGAKAAPAKTAGRKTTKKK
CCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond Between N-Acetylmuramic Acid And N-Acetylglucosamine Residues, Thereby Conserving The Energy In A Newly Synthesized 1,6-Anhydrobond In The Muramic Acid Residue. [C]

General reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]