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The map label for this gene is purD [H]

Identifier: 237811526

GI number: 237811526

Start: 1211675

End: 1212952

Strand: Reverse

Name: purD [H]

Synonym: GBP346_A1256

Alternate gene names: 237811526

Gene position: 1212952-1211675 (Counterclockwise)

Preceding gene: 237811527

Following gene: 237811525

Centisome position: 29.59

GC content: 68.15

Gene sequence:

>1278_bases
ATGAAACTACTCGTCGTCGGCTCCGGCGGCCGCGAACATGCGCTCGCCTGGAAGCTCGCCCAGTCGCCGCGCGTCCAGCT
CGTCTACGTTGCGCCCGGCAACGGCGGCACCGCGCAGGACGAGCGCCTGAAGAACGTCGATCTGAGCTCGCTCGACGATC
TGGCCGATTTCGCCGAATCCGAAGGCGTCGCGTTCACGCTCGTCGGCCCGGAAGCGCCGCTCGCGGCGGGCATCGTCAAT
CATTTCCGCGCGCGCGGCCTGAAGATCTTCGGCCCGACGAAGGAAGCGGCGCAGCTCGAGAGCTCGAAGGACTTCGCGAA
GGCGTTCATGAAGCGCCACGGCATTCCGACCGCCGATTACGAGACGTTCACCGACGCGGCCGCCGCGCACGCGTACGTCG
ATTCGAAAGGCGCGCCGATCGTCGTGAAGGCGGACGGCCTCGCCGCGGGCAAGGGCGTCGTCGTCGCGATGACGCTGGAA
GAAGCGCACGCGGCCGTCGACATGATGCTGTCCGGCAACAAGCTCGGCGATGCCGGCGCGCGCGTCGTGATCGAGGCATT
CCTCGACGGCGAGGAAGCGAGCTTCATCGTGATGGTCGACGGCAAGCACGCGCTCGCGCTCGCGTCGAGCCAGGATCACA
AGCGTCTGCTCGACGAGGACCGCGGCCCGAACACGGGCGGCATGGGCGCGTACTCGCCGGCGCCGATCGTCACGCCGCAG
ATGCATGCGCGCGTGATGCGCGAGATCATCATGCCGACCGTGCGCGGGATGGAGAAGGACGGCATCCGCTTCACGGGCTT
CCTGTACGCGGGCCTGATGATCGACCGCGACGGCAATCCGCGCACGCTCGAATTCAACTGCCGGATGGGCGACCCCGAAA
CGCAGCCGATCATGGCGCGCCTGAAGAGCGATTTCTCGAAGGTCGTCGAGCAGGCGATCGCGGGCACGCTCGATACCGTC
GAGCTCGACTGGGACCGCCGCACGGCGCTCGGCGTCGTGCTCGCCGCGCACGGCTACCCCGATGCGCCGCGCAAGGGCGA
CCGGATCAACGGCATCCCGGCAGAAACGGCGCACGCGGTGACGTTCCACGCGGGCACGACGTTCGACGGCGACAAGCTCG
TCACCTCCGGCGGGCGCGTGCTGTGCGTGGTCGGCCTCGCGGATTCGGTGCGCGGCGCGCAGCAGGCCGCGTACGAGACG
ATCAACCAGATCAACTTCGAAGGCATGCAGTATCGCCGCGACATCGGCTACCGCGCGCTCAACCGCAAGACGGTCTGA

Upstream 100 bases:

>100_bases
GCCGCCCTTTCGCGCGAAGGACGGCGGCCGCGGCCGTCTCTCGCGGCCGGCCCGCATCGCGCGCGCCGGCCGCCCTATTT
TCTAGTCTGCGGGGAATCCC

Downstream 100 bases:

>100_bases
CGCGCCGCTCGCCGCTCCTCGCGCGGCCGGCAAGCCGGCCGGCGCGCCCTCTCCTCTGCCGGGGTTCGCTAGAATGCCCG
GCAGAAGCATTTTTCGACGG

Product: phosphoribosylamine--glycine ligase

Products: NA

Alternate protein names: GARS; Glycinamide ribonucleotide synthetase; Phosphoribosylglycinamide synthetase [H]

Number of amino acids: Translated: 425; Mature: 425

Protein sequence:

>425_residues
MKLLVVGSGGREHALAWKLAQSPRVQLVYVAPGNGGTAQDERLKNVDLSSLDDLADFAESEGVAFTLVGPEAPLAAGIVN
HFRARGLKIFGPTKEAAQLESSKDFAKAFMKRHGIPTADYETFTDAAAAHAYVDSKGAPIVVKADGLAAGKGVVVAMTLE
EAHAAVDMMLSGNKLGDAGARVVIEAFLDGEEASFIVMVDGKHALALASSQDHKRLLDEDRGPNTGGMGAYSPAPIVTPQ
MHARVMREIIMPTVRGMEKDGIRFTGFLYAGLMIDRDGNPRTLEFNCRMGDPETQPIMARLKSDFSKVVEQAIAGTLDTV
ELDWDRRTALGVVLAAHGYPDAPRKGDRINGIPAETAHAVTFHAGTTFDGDKLVTSGGRVLCVVGLADSVRGAQQAAYET
INQINFEGMQYRRDIGYRALNRKTV

Sequences:

>Translated_425_residues
MKLLVVGSGGREHALAWKLAQSPRVQLVYVAPGNGGTAQDERLKNVDLSSLDDLADFAESEGVAFTLVGPEAPLAAGIVN
HFRARGLKIFGPTKEAAQLESSKDFAKAFMKRHGIPTADYETFTDAAAAHAYVDSKGAPIVVKADGLAAGKGVVVAMTLE
EAHAAVDMMLSGNKLGDAGARVVIEAFLDGEEASFIVMVDGKHALALASSQDHKRLLDEDRGPNTGGMGAYSPAPIVTPQ
MHARVMREIIMPTVRGMEKDGIRFTGFLYAGLMIDRDGNPRTLEFNCRMGDPETQPIMARLKSDFSKVVEQAIAGTLDTV
ELDWDRRTALGVVLAAHGYPDAPRKGDRINGIPAETAHAVTFHAGTTFDGDKLVTSGGRVLCVVGLADSVRGAQQAAYET
INQINFEGMQYRRDIGYRALNRKTV
>Mature_425_residues
MKLLVVGSGGREHALAWKLAQSPRVQLVYVAPGNGGTAQDERLKNVDLSSLDDLADFAESEGVAFTLVGPEAPLAAGIVN
HFRARGLKIFGPTKEAAQLESSKDFAKAFMKRHGIPTADYETFTDAAAAHAYVDSKGAPIVVKADGLAAGKGVVVAMTLE
EAHAAVDMMLSGNKLGDAGARVVIEAFLDGEEASFIVMVDGKHALALASSQDHKRLLDEDRGPNTGGMGAYSPAPIVTPQ
MHARVMREIIMPTVRGMEKDGIRFTGFLYAGLMIDRDGNPRTLEFNCRMGDPETQPIMARLKSDFSKVVEQAIAGTLDTV
ELDWDRRTALGVVLAAHGYPDAPRKGDRINGIPAETAHAVTFHAGTTFDGDKLVTSGGRVLCVVGLADSVRGAQQAAYET
INQINFEGMQYRRDIGYRALNRKTV

Specific function: De novo purine biosynthesis; second step. [C]

COG id: COG0151

COG function: function code F; Phosphoribosylamine-glycine ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Homo sapiens, GI28416899, Length=424, Percent_Identity=49.5283018867925, Blast_Score=401, Evalue=1e-112,
Organism=Homo sapiens, GI4503915, Length=424, Percent_Identity=49.5283018867925, Blast_Score=401, Evalue=1e-112,
Organism=Homo sapiens, GI209869995, Length=424, Percent_Identity=49.5283018867925, Blast_Score=401, Evalue=1e-112,
Organism=Homo sapiens, GI209869993, Length=424, Percent_Identity=49.5283018867925, Blast_Score=401, Evalue=1e-112,
Organism=Escherichia coli, GI1790438, Length=427, Percent_Identity=60.4215456674473, Blast_Score=515, Evalue=1e-147,
Organism=Caenorhabditis elegans, GI17567511, Length=421, Percent_Identity=44.6555819477435, Blast_Score=345, Evalue=3e-95,
Organism=Saccharomyces cerevisiae, GI6321203, Length=444, Percent_Identity=41.8918918918919, Blast_Score=346, Evalue=4e-96,
Organism=Drosophila melanogaster, GI62471619, Length=431, Percent_Identity=46.1716937354988, Blast_Score=354, Evalue=5e-98,
Organism=Drosophila melanogaster, GI24582400, Length=431, Percent_Identity=46.1716937354988, Blast_Score=353, Evalue=2e-97,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR020561
- InterPro:   IPR000115
- InterPro:   IPR020560
- InterPro:   IPR020562
- InterPro:   IPR011054 [H]

Pfam domain/function: PF01071 GARS_A; PF02843 GARS_C; PF02844 GARS_N [H]

EC number: =6.3.4.13 [H]

Molecular weight: Translated: 45477; Mature: 45477

Theoretical pI: Translated: 6.30; Mature: 6.30

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLLVVGSGGREHALAWKLAQSPRVQLVYVAPGNGGTAQDERLKNVDLSSLDDLADFAES
CEEEEEECCCCCCEEEEEECCCCCEEEEEEECCCCCCCCHHHHCCCCCHHHHHHHHHHHC
EGVAFTLVGPEAPLAAGIVNHFRARGLKIFGPTKEAAQLESSKDFAKAFMKRHGIPTADY
CCEEEEEECCCCCHHHHHHHHHHHCCEEEECCCHHHHHHHCCHHHHHHHHHHCCCCCCCC
ETFTDAAAAHAYVDSKGAPIVVKADGLAAGKGVVVAMTLEEAHAAVDMMLSGNKLGDAGA
HHHHHHHHHHHEECCCCCEEEEEECCCCCCCCEEEEEEHHHHHHHHHHEECCCCCCCCHH
RVVIEAFLDGEEASFIVMVDGKHALALASSQDHKRLLDEDRGPNTGGMGAYSPAPIVTPQ
EEEEEEEECCCCCEEEEEECCCEEEEECCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCHH
MHARVMREIIMPTVRGMEKDGIRFTGFLYAGLMIDRDGNPRTLEFNCRMGDPETQPIMAR
HHHHHHHHHHHHHHCCCCCCCCEEEEEEEEEEEEECCCCCCEEEEEEECCCCCCHHHHHH
LKSDFSKVVEQAIAGTLDTVELDWDRRTALGVVLAAHGYPDAPRKGDRINGIPAETAHAV
HHHHHHHHHHHHHCCCCEEEEECCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCCEEE
TFHAGTTFDGDKLVTSGGRVLCVVGLADSVRGAQQAAYETINQINFEGMQYRRDIGYRAL
EEECCCCCCCCEEEECCCCEEEEEECCHHHHHHHHHHHHHHHHCCCCCHHHHHHHCHHHH
NRKTV
CCCCC
>Mature Secondary Structure
MKLLVVGSGGREHALAWKLAQSPRVQLVYVAPGNGGTAQDERLKNVDLSSLDDLADFAES
CEEEEEECCCCCCEEEEEECCCCCEEEEEEECCCCCCCCHHHHCCCCCHHHHHHHHHHHC
EGVAFTLVGPEAPLAAGIVNHFRARGLKIFGPTKEAAQLESSKDFAKAFMKRHGIPTADY
CCEEEEEECCCCCHHHHHHHHHHHCCEEEECCCHHHHHHHCCHHHHHHHHHHCCCCCCCC
ETFTDAAAAHAYVDSKGAPIVVKADGLAAGKGVVVAMTLEEAHAAVDMMLSGNKLGDAGA
HHHHHHHHHHHEECCCCCEEEEEECCCCCCCCEEEEEEHHHHHHHHHHEECCCCCCCCHH
RVVIEAFLDGEEASFIVMVDGKHALALASSQDHKRLLDEDRGPNTGGMGAYSPAPIVTPQ
EEEEEEEECCCCCEEEEEECCCEEEEECCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCHH
MHARVMREIIMPTVRGMEKDGIRFTGFLYAGLMIDRDGNPRTLEFNCRMGDPETQPIMAR
HHHHHHHHHHHHHHCCCCCCCCEEEEEEEEEEEEECCCCCCEEEEEEECCCCCCHHHHHH
LKSDFSKVVEQAIAGTLDTVELDWDRRTALGVVLAAHGYPDAPRKGDRINGIPAETAHAV
HHHHHHHHHHHHHCCCCEEEEECCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCCEEE
TFHAGTTFDGDKLVTSGGRVLCVVGLADSVRGAQQAAYETINQINFEGMQYRRDIGYRAL
EEECCCCCCCCEEEECCCCEEEEEECCHHHHHHHHHHHHHHHHCCCCCHHHHHHHCHHHH
NRKTV
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11823852 [H]