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The map label for this gene is nth [H]

Identifier: 229918737

GI number: 229918737

Start: 2982880

End: 2983539

Strand: Direct

Name: nth [H]

Synonym: EAT1b_3024

Alternate gene names: 229918737

Gene position: 2982880-2983539 (Clockwise)

Preceding gene: 229918736

Following gene: 229918738

Centisome position: 99.43

GC content: 49.7

Gene sequence:

>660_bases
ATGTTGACGCGAGCACAATTACAGGAAGTGTCAGATACGATGAAGCAGATGTTCCCGGATGCTCATTGCGAGTTGACGCA
TCAAAATCCATTCGAATTGGTGGTCGCCGTCGCGCTCTCCGCACAAGCGACGGATGCACTCGTCAACAAGGTGACACCGG
GTTTGTTTGAAGCGTTTCCGACTGTCGAGGCGATGGCGGCGGCTGACGTGTCTGAGATTGAGGCATTGATTAAACGGATT
GGTCTGTACCGTAACAAAGCGAAGAATGTAAAAGCACTCTCAGAAAAAATTGTGAACGAGCATGGTGGAATTGTCCCTTC
TGACCGAGCATCGTTGGAGGCGCTCCCTGGAGTCGGTCGTAAGACAGCCAATGTCGTTCTCTCTGTTGCATTCCATGAAC
CGGCGTTTGCGGTTGACACGCATGTGGAACGAGTATCCAAACGTCTCGGTATTTGTCGCTGGAAAGACAATGTCCGCCAA
GTCGAGGATACATTAATGAAAAAGTTTCCGCGCGAGGAATGGTCACAACTTCATCATCAATTCATCTTCTTCGGACGATA
TCATTGTAAGGCACAACGTCCGGGCTGTGAGGCATGCCCACTGTTGCATATGTGCCGAGAAGGAAAGAAACGAATGAAGT
CACAGCAAGTCACCACATAA

Upstream 100 bases:

>100_bases
TGCGAAACTTCAAATACATGGACAAAATCCTGCGTGTTTGGCAAGATCATCATGTCGAATCGCTCGAGGATGTCGTGGAA
TATCAACGGAGGAATAAGTG

Downstream 100 bases:

>100_bases
AAACGCATCGACACGAAAGGTGTCGATGCGTCAGACTGTAGACAAAGTATCGGTCTAATGTCTTTTATATGCCCAACCCG
GACACGGCTCGCTTTCCTAG

Product: endonuclease III

Products: NA

Alternate protein names: DNA-(apurinic or apyrimidinic site) lyase [H]

Number of amino acids: Translated: 219; Mature: 219

Protein sequence:

>219_residues
MLTRAQLQEVSDTMKQMFPDAHCELTHQNPFELVVAVALSAQATDALVNKVTPGLFEAFPTVEAMAAADVSEIEALIKRI
GLYRNKAKNVKALSEKIVNEHGGIVPSDRASLEALPGVGRKTANVVLSVAFHEPAFAVDTHVERVSKRLGICRWKDNVRQ
VEDTLMKKFPREEWSQLHHQFIFFGRYHCKAQRPGCEACPLLHMCREGKKRMKSQQVTT

Sequences:

>Translated_219_residues
MLTRAQLQEVSDTMKQMFPDAHCELTHQNPFELVVAVALSAQATDALVNKVTPGLFEAFPTVEAMAAADVSEIEALIKRI
GLYRNKAKNVKALSEKIVNEHGGIVPSDRASLEALPGVGRKTANVVLSVAFHEPAFAVDTHVERVSKRLGICRWKDNVRQ
VEDTLMKKFPREEWSQLHHQFIFFGRYHCKAQRPGCEACPLLHMCREGKKRMKSQQVTT
>Mature_219_residues
MLTRAQLQEVSDTMKQMFPDAHCELTHQNPFELVVAVALSAQATDALVNKVTPGLFEAFPTVEAMAAADVSEIEALIKRI
GLYRNKAKNVKALSEKIVNEHGGIVPSDRASLEALPGVGRKTANVVLSVAFHEPAFAVDTHVERVSKRLGICRWKDNVRQ
VEDTLMKKFPREEWSQLHHQFIFFGRYHCKAQRPGCEACPLLHMCREGKKRMKSQQVTT

Specific function: Has Both An Apurinic And/Or Apyrimidinic Endonuclease Activity And A DNA N-Glycosylase Activity. Incises Damaged DNA At Cytosines, Thymines And Guanines. Acts On A Damaged Strand, 5' From The Damaged Site. Required For The Repair Of Both Oxidative DNA Da

COG id: COG0177

COG function: function code L; Predicted EndoIII-related endonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Nth/MutY family [H]

Homologues:

Organism=Homo sapiens, GI4505471, Length=187, Percent_Identity=32.0855614973262, Blast_Score=95, Evalue=4e-20,
Organism=Homo sapiens, GI115298654, Length=198, Percent_Identity=28.2828282828283, Blast_Score=75, Evalue=3e-14,
Organism=Homo sapiens, GI115298652, Length=198, Percent_Identity=28.2828282828283, Blast_Score=75, Evalue=3e-14,
Organism=Homo sapiens, GI115298648, Length=198, Percent_Identity=28.2828282828283, Blast_Score=75, Evalue=3e-14,
Organism=Homo sapiens, GI115298650, Length=198, Percent_Identity=28.2828282828283, Blast_Score=75, Evalue=3e-14,
Organism=Homo sapiens, GI6912520, Length=198, Percent_Identity=28.2828282828283, Blast_Score=75, Evalue=3e-14,
Organism=Homo sapiens, GI190358497, Length=198, Percent_Identity=28.2828282828283, Blast_Score=75, Evalue=4e-14,
Organism=Escherichia coli, GI1787920, Length=209, Percent_Identity=44.4976076555024, Blast_Score=169, Evalue=1e-43,
Organism=Escherichia coli, GI1789331, Length=183, Percent_Identity=26.775956284153, Blast_Score=70, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17554540, Length=177, Percent_Identity=30.5084745762712, Blast_Score=96, Evalue=1e-20,
Organism=Drosophila melanogaster, GI45550361, Length=177, Percent_Identity=32.2033898305085, Blast_Score=97, Evalue=1e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011257
- InterPro:   IPR004036
- InterPro:   IPR005759
- InterPro:   IPR004035
- InterPro:   IPR003651
- InterPro:   IPR003265
- InterPro:   IPR000445
- InterPro:   IPR003583
- InterPro:   IPR023170 [H]

Pfam domain/function: PF00633 HHH; PF00730 HhH-GPD [H]

EC number: =4.2.99.18 [H]

Molecular weight: Translated: 24592; Mature: 24592

Theoretical pI: Translated: 8.84; Mature: 8.84

Prosite motif: PS00764 ENDONUCLEASE_III_1 ; PS01155 ENDONUCLEASE_III_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTRAQLQEVSDTMKQMFPDAHCELTHQNPFELVVAVALSAQATDALVNKVTPGLFEAFP
CCCHHHHHHHHHHHHHHCCCCCEECCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
TVEAMAAADVSEIEALIKRIGLYRNKAKNVKALSEKIVNEHGGIVPSDRASLEALPGVGR
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHCCCCCC
KTANVVLSVAFHEPAFAVDTHVERVSKRLGICRWKDNVRQVEDTLMKKFPREEWSQLHHQ
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
FIFFGRYHCKAQRPGCEACPLLHMCREGKKRMKSQQVTT
HHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MLTRAQLQEVSDTMKQMFPDAHCELTHQNPFELVVAVALSAQATDALVNKVTPGLFEAFP
CCCHHHHHHHHHHHHHHCCCCCEECCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
TVEAMAAADVSEIEALIKRIGLYRNKAKNVKALSEKIVNEHGGIVPSDRASLEALPGVGR
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHCCCCCC
KTANVVLSVAFHEPAFAVDTHVERVSKRLGICRWKDNVRQVEDTLMKKFPREEWSQLHHQ
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
FIFFGRYHCKAQRPGCEACPLLHMCREGKKRMKSQQVTT
HHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7704260; 8760912; 9384377 [H]