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The map label for this gene is asnC [H]

Identifier: 229918735

GI number: 229918735

Start: 2980926

End: 2982233

Strand: Direct

Name: asnC [H]

Synonym: EAT1b_3022

Alternate gene names: 229918735

Gene position: 2980926-2982233 (Clockwise)

Preceding gene: 229918734

Following gene: 229918736

Centisome position: 99.37

GC content: 46.79

Gene sequence:

>1308_bases
ATGAACATGACAACTATTTCGATTAACCAATTTAAAGACTATGAAGGCCAATCTGTCCGTGTAGGCGCGTGGATTGCCAA
CAAACGTTCAAGTGGAAAGATTGCTTTCCTTCAATTACGTGACGGTAGCGGTTTTGCTCAAGCGGTCGTTGTAAAAGCTG
ATGTGGCGGAAGAGTTATTTACTCTTGCCAAAGGGATCACGCAAGAAACGTCGGTTTGGGTCACAGGGAATGTCAAATCT
GATGGTGGACGTACACCACTCGGATTTGAATTGGAAGTGACAGATTTAGAAGTGATTGCGGAATCGGTTGATTATCCAAT
CACACCGAAAGAACACGGAACGGAATTCTTGATGGACAACCGTCACTTATGGCTCCGTTCACGTCGCCAACACGCGATTA
TGAAAGTTCGTAACGAAATCATCCGTGCGACATATGAATTCTTCAACAAAGAAGGATTCGTCAAAATCGATCCGCCAATC
CTTACGAGCAGTGCACCGGAAGGGACGACAGAATTGTTCGAGACAGACTATTTCGGTCAGTCAGCCTACCTTTCTCAGAC
GGGTCAATTGTACATGGAAGCCGCGGCAATGGCACTCGGTAAAGTATTCTCGTTCGGACCGACGTTCCGTGCAGAGAAAT
CAAAAACGCGTCGTCACTTGATTGAGTTCTGGATGATCGAGCCGGAGATGGCATTTGTCGATCATGACATGAACCTCGAG
ATTCAAGAGCAGTACGTCTCATACATTGCACAGTCGGCACTTGCGAACTGTAAAAATGAATTGGAACTACTCGGACGTGA
CCTTGAGAAATTGGCACAAGTACAAGCGCCGTTCCCACGCGTTCGTTACGAAGATGCCATCAAGTTCTTGAAGGCAGAAG
GATTCGACGACATCGAATACGGGGATGATTTCGGTGCACCGCACGAGACGGCGATTGCCAACGCACATGATCAGCCGGTA
TTCATCACGCATTGGCCAAAAGAAATCAAACCGTTCTATATGAAAGTAGACCCTGAAAATCCTGAGCTTGTTCTTTGTGC
GGACTTGATTGCTCCAGAAGGATATGGTGAAATCATCGGGGGATCTCAACGTGAGGACGACTACGATCGTCTAAAAGAAG
AAATCATCAAAGAAGGTCTCGACGAAACGGATGCGTATGATTGGTACCTTGAACTTCGTCAATACGGTTCGGTCCCACAC
TCTGGTTTCGGACTTGGTCTCGAGCGTACAGTCGCATGGATCAGTGGAATCGAACATATCCGTGAAGCGATTCCATTCCC
ACGTTTATTAAACCGAATTCACCCTTAA

Upstream 100 bases:

>100_bases
TACGATTATCCTATGCAACTGAATTAAAACGCGTATTAGAAGCGCTCGATCGAGTAGAAGACTTCGTTACGAAGCGCGCT
CGATAAGCGAAAGGATGGAA

Downstream 100 bases:

>100_bases
CGATTAATCGGGGAAGACGTCATGTCCTCCCCGATTGATGTGTCTTAGAAAGGAAGTGCGACATGGATGCACATCAATTG
ATTCAATTATTCGAACAAAG

Product: asparaginyl-tRNA synthetase

Products: NA

Alternate protein names: Asparagine--tRNA ligase; AsnRS [H]

Number of amino acids: Translated: 435; Mature: 435

Protein sequence:

>435_residues
MNMTTISINQFKDYEGQSVRVGAWIANKRSSGKIAFLQLRDGSGFAQAVVVKADVAEELFTLAKGITQETSVWVTGNVKS
DGGRTPLGFELEVTDLEVIAESVDYPITPKEHGTEFLMDNRHLWLRSRRQHAIMKVRNEIIRATYEFFNKEGFVKIDPPI
LTSSAPEGTTELFETDYFGQSAYLSQTGQLYMEAAAMALGKVFSFGPTFRAEKSKTRRHLIEFWMIEPEMAFVDHDMNLE
IQEQYVSYIAQSALANCKNELELLGRDLEKLAQVQAPFPRVRYEDAIKFLKAEGFDDIEYGDDFGAPHETAIANAHDQPV
FITHWPKEIKPFYMKVDPENPELVLCADLIAPEGYGEIIGGSQREDDYDRLKEEIIKEGLDETDAYDWYLELRQYGSVPH
SGFGLGLERTVAWISGIEHIREAIPFPRLLNRIHP

Sequences:

>Translated_435_residues
MNMTTISINQFKDYEGQSVRVGAWIANKRSSGKIAFLQLRDGSGFAQAVVVKADVAEELFTLAKGITQETSVWVTGNVKS
DGGRTPLGFELEVTDLEVIAESVDYPITPKEHGTEFLMDNRHLWLRSRRQHAIMKVRNEIIRATYEFFNKEGFVKIDPPI
LTSSAPEGTTELFETDYFGQSAYLSQTGQLYMEAAAMALGKVFSFGPTFRAEKSKTRRHLIEFWMIEPEMAFVDHDMNLE
IQEQYVSYIAQSALANCKNELELLGRDLEKLAQVQAPFPRVRYEDAIKFLKAEGFDDIEYGDDFGAPHETAIANAHDQPV
FITHWPKEIKPFYMKVDPENPELVLCADLIAPEGYGEIIGGSQREDDYDRLKEEIIKEGLDETDAYDWYLELRQYGSVPH
SGFGLGLERTVAWISGIEHIREAIPFPRLLNRIHP
>Mature_435_residues
MNMTTISINQFKDYEGQSVRVGAWIANKRSSGKIAFLQLRDGSGFAQAVVVKADVAEELFTLAKGITQETSVWVTGNVKS
DGGRTPLGFELEVTDLEVIAESVDYPITPKEHGTEFLMDNRHLWLRSRRQHAIMKVRNEIIRATYEFFNKEGFVKIDPPI
LTSSAPEGTTELFETDYFGQSAYLSQTGQLYMEAAAMALGKVFSFGPTFRAEKSKTRRHLIEFWMIEPEMAFVDHDMNLE
IQEQYVSYIAQSALANCKNELELLGRDLEKLAQVQAPFPRVRYEDAIKFLKAEGFDDIEYGDDFGAPHETAIANAHDQPV
FITHWPKEIKPFYMKVDPENPELVLCADLIAPEGYGEIIGGSQREDDYDRLKEEIIKEGLDETDAYDWYLELRQYGSVPH
SGFGLGLERTVAWISGIEHIREAIPFPRLLNRIHP

Specific function: Unknown

COG id: COG0017

COG function: function code J; Aspartyl/asparaginyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI226491502, Length=445, Percent_Identity=35.7303370786517, Blast_Score=254, Evalue=8e-68,
Organism=Homo sapiens, GI4758762, Length=449, Percent_Identity=34.7438752783964, Blast_Score=252, Evalue=4e-67,
Organism=Homo sapiens, GI45439306, Length=449, Percent_Identity=30.2895322939866, Blast_Score=166, Evalue=5e-41,
Organism=Escherichia coli, GI1787161, Length=448, Percent_Identity=36.8303571428571, Blast_Score=279, Evalue=3e-76,
Organism=Escherichia coli, GI1790571, Length=453, Percent_Identity=24.9448123620309, Blast_Score=98, Evalue=1e-21,
Organism=Escherichia coli, GI1789256, Length=453, Percent_Identity=25.1655629139073, Blast_Score=97, Evalue=3e-21,
Organism=Escherichia coli, GI1788173, Length=245, Percent_Identity=26.1224489795918, Blast_Score=75, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI71984122, Length=445, Percent_Identity=35.9550561797753, Blast_Score=259, Evalue=2e-69,
Organism=Caenorhabditis elegans, GI17556372, Length=414, Percent_Identity=31.4009661835749, Blast_Score=171, Evalue=8e-43,
Organism=Caenorhabditis elegans, GI17551876, Length=457, Percent_Identity=28.6652078774617, Blast_Score=149, Evalue=3e-36,
Organism=Caenorhabditis elegans, GI32566633, Length=287, Percent_Identity=25.4355400696864, Blast_Score=74, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6321807, Length=436, Percent_Identity=36.9266055045872, Blast_Score=269, Evalue=7e-73,
Organism=Saccharomyces cerevisiae, GI6319872, Length=459, Percent_Identity=32.6797385620915, Blast_Score=227, Evalue=2e-60,
Organism=Saccharomyces cerevisiae, GI6323011, Length=469, Percent_Identity=28.1449893390192, Blast_Score=144, Evalue=3e-35,
Organism=Drosophila melanogaster, GI19921528, Length=443, Percent_Identity=34.9887133182844, Blast_Score=238, Evalue=5e-63,
Organism=Drosophila melanogaster, GI19922416, Length=452, Percent_Identity=32.9646017699115, Blast_Score=216, Evalue=3e-56,
Organism=Drosophila melanogaster, GI17136276, Length=451, Percent_Identity=29.7117516629712, Blast_Score=157, Evalue=1e-38,

Paralogues:

None

Copy number: 80 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR004522
- InterPro:   IPR002312
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365 [H]

Pfam domain/function: PF00152 tRNA-synt_2; PF01336 tRNA_anti [H]

EC number: =6.1.1.22 [H]

Molecular weight: Translated: 49435; Mature: 49435

Theoretical pI: Translated: 4.63; Mature: 4.63

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNMTTISINQFKDYEGQSVRVGAWIANKRSSGKIAFLQLRDGSGFAQAVVVKADVAEELF
CCEEEEEHHHCCCCCCCEEEEEEEEECCCCCCCEEEEEEECCCCCEEEEEEHHHHHHHHH
TLAKGITQETSVWVTGNVKSDGGRTPLGFELEVTDLEVIAESVDYPITPKEHGTEFLMDN
HHHHCCCCCCEEEEEEEEECCCCCCCCCEEEEEHHHHHHHHHCCCCCCCCCCCCHHHCCC
RHLWLRSRRQHAIMKVRNEIIRATYEFFNKEGFVKIDPPILTSSAPEGTTELFETDYFGQ
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCEECCCCCCCCHHHHHHCCCCC
SAYLSQTGQLYMEAAAMALGKVFSFGPTFRAEKSKTRRHLIEFWMIEPEMAFVDHDMNLE
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHEECCCCEEECCCCCCH
IQEQYVSYIAQSALANCKNELELLGRDLEKLAQVQAPFPRVRYEDAIKFLKAEGFDDIEY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCCC
GDDFGAPHETAIANAHDQPVFITHWPKEIKPFYMKVDPENPELVLCADLIAPEGYGEIIG
CCCCCCCCHHHCCCCCCCCEEEEECCCCCCCEEEEECCCCCCEEEEEHHHCCCCCCHHCC
GSQREDDYDRLKEEIIKEGLDETDAYDWYLELRQYGSVPHSGFGLGLERTVAWISGIEHI
CCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHH
REAIPFPRLLNRIHP
HHHCCHHHHHHHCCC
>Mature Secondary Structure
MNMTTISINQFKDYEGQSVRVGAWIANKRSSGKIAFLQLRDGSGFAQAVVVKADVAEELF
CCEEEEEHHHCCCCCCCEEEEEEEEECCCCCCCEEEEEEECCCCCEEEEEEHHHHHHHHH
TLAKGITQETSVWVTGNVKSDGGRTPLGFELEVTDLEVIAESVDYPITPKEHGTEFLMDN
HHHHCCCCCCEEEEEEEEECCCCCCCCCEEEEEHHHHHHHHHCCCCCCCCCCCCHHHCCC
RHLWLRSRRQHAIMKVRNEIIRATYEFFNKEGFVKIDPPILTSSAPEGTTELFETDYFGQ
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCEECCCCCCCCHHHHHHCCCCC
SAYLSQTGQLYMEAAAMALGKVFSFGPTFRAEKSKTRRHLIEFWMIEPEMAFVDHDMNLE
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHEECCCCEEECCCCCCH
IQEQYVSYIAQSALANCKNELELLGRDLEKLAQVQAPFPRVRYEDAIKFLKAEGFDDIEY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCCC
GDDFGAPHETAIANAHDQPVFITHWPKEIKPFYMKVDPENPELVLCADLIAPEGYGEIIG
CCCCCCCCHHHCCCCCCCCEEEEECCCCCCCEEEEECCCCCCEEEEEHHHCCCCCCHHCC
GSQREDDYDRLKEEIIKEGLDETDAYDWYLELRQYGSVPHSGFGLGLERTVAWISGIEHI
CCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHH
REAIPFPRLLNRIHP
HHHCCHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA