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The map label for this gene is birA [H]

Identifier: 229918727

GI number: 229918727

Start: 2972813

End: 2973775

Strand: Direct

Name: birA [H]

Synonym: EAT1b_3014

Alternate gene names: 229918727

Gene position: 2972813-2973775 (Clockwise)

Preceding gene: 229918726

Following gene: 229918728

Centisome position: 99.1

GC content: 51.61

Gene sequence:

>963_bases
ATGGCTTCAACGCGCGGGCGTGTTCTGACGCTATTACAAGATGGTGAAGTATGGAGTGGTCAAGAGTTGGCACGCCAATT
AAATATTTCACGTACCGCTATTTGGAAACATATCGAAACGCTTAAGCAAGACGGCTATGCGATTCATACAATCCCAAGAA
AAGGATATCAATTGCTTGAACAAAGTGACCGATTCGATGAACCAGCGTTTGCGTCATATCGGAAGGGACGGTTCGGTCAA
ATCGTCCATGCGTTTGACCAAATCGACACGACACAACGAATCGCCCATGAGCTGGCGCAACAACACGTGCCAGAGGGGAC
GCTCGTCATGAGCGAGGAACAGACGGCAGGGCGCGGACAACTCGGTCGCAATTGGTACAATCCGAAGCACGTCAATATCG
CGGCAAGTGTGATTTTAAGGCCGGAGTTACCCATTCGCGACGCATCAAAGCTTACCTTGATGGCGGCGAGTGCGTTTGCG
AAAGCGTTGAATGCGCGTGACCTTGACGTGACCATCAAATGGCCAAACGATCTACTGCTAAATGGCAAGAAAATCGGAGG
CATTCTGACAGAAATGCAAACGGAAGGTGACCGCATCCAAGCCGTCATTTTAGGGTTCGGAATGAACGTCAACGGCGATG
ACATTCCGACGGAGTTGGCGCACCGGGCGACCTCCCTGTATTTAGAGACCAGGACGCGGTATCATCGTAGTGAGTTATTG
GCAGAACTGTTGGCAGAACTCGAGACCGAATACGATCGGTTCCTTGACGTGGGATTTGCGCCCGTTCGCTTGAACTGGTT
GAAACATGCCGCCTATTTAAATGAGTCGGTCACCTTGAGTGGGTCCCGAACGTCGCGAACAGGTGTCATGCGTGGCATCT
CGGACGAGGGGGCGCTACTACTCGAAGTGGATGGAACGGTCGAACCGGTCTATTCTGCTGAGATTGCAGTGTGGAATGAT
TGA

Upstream 100 bases:

>100_bases
GAGGGGAAGCGATCAAGCATCTGTTAGCCGACCTCGAACGACGCGTCATCTTAAACGAGTTACCGAACCATGAGACAGTA
TTGAAAGAGGAGGCGAAACG

Downstream 100 bases:

>100_bases
TGAAAGCGAGACCATCGACTATACTTAATGTGTCGGACGCTATCAAGTTTTGAAGTGTACCGCGCTTGCAGACTGTCACA
TTCAGTTGTCTGCCTTGATC

Product: biotin/acetyl-CoA-carboxylase ligase

Products: NA

Alternate protein names: Biotin operon repressor; Biotin--[acetyl-CoA-carboxylase] synthetase; Biotin--protein ligase [H]

Number of amino acids: Translated: 320; Mature: 319

Protein sequence:

>320_residues
MASTRGRVLTLLQDGEVWSGQELARQLNISRTAIWKHIETLKQDGYAIHTIPRKGYQLLEQSDRFDEPAFASYRKGRFGQ
IVHAFDQIDTTQRIAHELAQQHVPEGTLVMSEEQTAGRGQLGRNWYNPKHVNIAASVILRPELPIRDASKLTLMAASAFA
KALNARDLDVTIKWPNDLLLNGKKIGGILTEMQTEGDRIQAVILGFGMNVNGDDIPTELAHRATSLYLETRTRYHRSELL
AELLAELETEYDRFLDVGFAPVRLNWLKHAAYLNESVTLSGSRTSRTGVMRGISDEGALLLEVDGTVEPVYSAEIAVWND

Sequences:

>Translated_320_residues
MASTRGRVLTLLQDGEVWSGQELARQLNISRTAIWKHIETLKQDGYAIHTIPRKGYQLLEQSDRFDEPAFASYRKGRFGQ
IVHAFDQIDTTQRIAHELAQQHVPEGTLVMSEEQTAGRGQLGRNWYNPKHVNIAASVILRPELPIRDASKLTLMAASAFA
KALNARDLDVTIKWPNDLLLNGKKIGGILTEMQTEGDRIQAVILGFGMNVNGDDIPTELAHRATSLYLETRTRYHRSELL
AELLAELETEYDRFLDVGFAPVRLNWLKHAAYLNESVTLSGSRTSRTGVMRGISDEGALLLEVDGTVEPVYSAEIAVWND
>Mature_319_residues
ASTRGRVLTLLQDGEVWSGQELARQLNISRTAIWKHIETLKQDGYAIHTIPRKGYQLLEQSDRFDEPAFASYRKGRFGQI
VHAFDQIDTTQRIAHELAQQHVPEGTLVMSEEQTAGRGQLGRNWYNPKHVNIAASVILRPELPIRDASKLTLMAASAFAK
ALNARDLDVTIKWPNDLLLNGKKIGGILTEMQTEGDRIQAVILGFGMNVNGDDIPTELAHRATSLYLETRTRYHRSELLA
ELLAELETEYDRFLDVGFAPVRLNWLKHAAYLNESVTLSGSRTSRTGVMRGISDEGALLLEVDGTVEPVYSAEIAVWND

Specific function: BirA acts both as a biotin-operon repressor and as the enzyme that synthesizes the corepressor, acetyl-CoA:carbon-dioxide ligase. This protein also activates biotin to form biotinyl-5'- adenylate and transfers the biotin moiety to biotin-accepting protein

COG id: COG0340

COG function: function code H; Biotin-(acetyl-CoA carboxylase) ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the biotin--protein ligase family [H]

Homologues:

Organism=Escherichia coli, GI1790408, Length=312, Percent_Identity=31.0897435897436, Blast_Score=145, Evalue=3e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: =6.3.4.15 [H]

Molecular weight: Translated: 35835; Mature: 35704

Theoretical pI: Translated: 6.06; Mature: 6.06

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASTRGRVLTLLQDGEVWSGQELARQLNISRTAIWKHIETLKQDGYAIHTIPRKGYQLLE
CCCCCCCEEEEEECCCCCCHHHHHHHHCCHHHHHHHHHHHHHHCCEEEEECCHHHHHHHH
QSDRFDEPAFASYRKGRFGQIVHAFDQIDTTQRIAHELAQQHVPEGTLVMSEEQTAGRGQ
HCCCCCCCHHHHHCCCCHHHHHHHHHHCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCCC
LGRNWYNPKHVNIAASVILRPELPIRDASKLTLMAASAFAKALNARDLDVTIKWPNDLLL
CCCCCCCCCEEEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEECCCCEEE
NGKKIGGILTEMQTEGDRIQAVILGFGMNVNGDDIPTELAHRATSLYLETRTRYHRSELL
CCHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
AELLAELETEYDRFLDVGFAPVRLNWLKHAAYLNESVTLSGSRTSRTGVMRGISDEGALL
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCEEEECCCCCCCHHHHCCCCCCCEEE
LEVDGTVEPVYSAEIAVWND
EEECCCCCCCEEEEEEEECC
>Mature Secondary Structure 
ASTRGRVLTLLQDGEVWSGQELARQLNISRTAIWKHIETLKQDGYAIHTIPRKGYQLLE
CCCCCCEEEEEECCCCCCHHHHHHHHCCHHHHHHHHHHHHHHCCEEEEECCHHHHHHHH
QSDRFDEPAFASYRKGRFGQIVHAFDQIDTTQRIAHELAQQHVPEGTLVMSEEQTAGRGQ
HCCCCCCCHHHHHCCCCHHHHHHHHHHCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCCC
LGRNWYNPKHVNIAASVILRPELPIRDASKLTLMAASAFAKALNARDLDVTIKWPNDLLL
CCCCCCCCCEEEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEECCCCEEE
NGKKIGGILTEMQTEGDRIQAVILGFGMNVNGDDIPTELAHRATSLYLETRTRYHRSELL
CCHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
AELLAELETEYDRFLDVGFAPVRLNWLKHAAYLNESVTLSGSRTSRTGVMRGISDEGALL
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCEEEECCCCCCCHHHHCCCCCCCEEE
LEVDGTVEPVYSAEIAVWND
EEECCCCCCCEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7730294 [H]