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The map label for this gene is panB

Identifier: 229918728

GI number: 229918728

Start: 2974001

End: 2974834

Strand: Direct

Name: panB

Synonym: EAT1b_3015

Alternate gene names: 229918728

Gene position: 2974001-2974834 (Clockwise)

Preceding gene: 229918727

Following gene: 229918729

Centisome position: 99.14

GC content: 49.88

Gene sequence:

>834_bases
ATGCACACGATGACTACATTGTTAAAAAAGATGAACGAGCAGGAAAAGCTCGTCATGTTGACGGCGTATGATTATCCGTC
AGCCAAACTTGCCGAATCGGCAGGGGTCGATTTGATTTTAGTAGGTGATTCACTCGGAATGGTCGTCCTTGGTTATGATT
CGACGATTCCAGTGACGATGGAGGACATGCTTCATCATTCGAAAGCCGTACGCCGAGGAGCACAACATACGTTCGTTGTG
GTGGATATGCCGTTTGCGTCATATCACGGAGACTTCGATCGAACGTTACAGGCAGCATCGCGCTTATTCCAAGAAGGTCG
TGCGGATGCCATTAAACTAGAAGGTGCCGGTGACGTGCTTCAGACGATTGAGCGTTTGACACAAGTCGGCATGCCATGTG
TCGCCCATTTAGGGTTGACCCCTCAATCGGTTGGCGTCCTAGAAGGATATAAAGTACAAGGGAAATCCCTTGAGGCTGCC
GAGACACTCCTTCAAGACAGCTTGGCTGCTGAACGTGCTGGCGCGAAGATGCTCGTCCTTGAGTGTGTCCCTCATCAATT
GGCAAAACGGATCGCAGAAGAGTTGACGATTCCTGTCATCGGAATCGGTGCAGGTGCAGATGTCGACGGCCAAGTGCTCG
TCTATCATGACGTATTGAAATATGGGGTGGACCGTCTCCCTAAATTTGTCCAAGCGTATGCAGACTTGAATGAAGTGGCG
ACGGATGCCATCCGCTCTTATGTCGAAGAGACGAAGAACGGTCACTTCCCACAAGAACGTCATACGTTCACAATGGATGA
GTCGTTGCTCGACTCGTTGTATGGAGGAAAATGA

Upstream 100 bases:

>100_bases
AACTCATGAAAACCATGAATTTGTTTTTCGTGCGCCTTCTTGCCATCTGTGTAAGAGGGCGTTTATTCTTTCCTGTCTCC
ACGTTTAGGAGGAGAAACAG

Downstream 100 bases:

>100_bases
TGAGAATTGTATCGACCGTCGAAGAATTACGTAGTGCTTTACCAATGGATCAAACGATTGGGTTCGTGCCGACGATGGGA
TATTTGCATGAAGGACATAT

Product: 3-methyl-2-oxobutanoate hydroxymethyltransferase

Products: NA

Alternate protein names: Ketopantoate hydroxymethyltransferase; KPHMT

Number of amino acids: Translated: 277; Mature: 277

Protein sequence:

>277_residues
MHTMTTLLKKMNEQEKLVMLTAYDYPSAKLAESAGVDLILVGDSLGMVVLGYDSTIPVTMEDMLHHSKAVRRGAQHTFVV
VDMPFASYHGDFDRTLQAASRLFQEGRADAIKLEGAGDVLQTIERLTQVGMPCVAHLGLTPQSVGVLEGYKVQGKSLEAA
ETLLQDSLAAERAGAKMLVLECVPHQLAKRIAEELTIPVIGIGAGADVDGQVLVYHDVLKYGVDRLPKFVQAYADLNEVA
TDAIRSYVEETKNGHFPQERHTFTMDESLLDSLYGGK

Sequences:

>Translated_277_residues
MHTMTTLLKKMNEQEKLVMLTAYDYPSAKLAESAGVDLILVGDSLGMVVLGYDSTIPVTMEDMLHHSKAVRRGAQHTFVV
VDMPFASYHGDFDRTLQAASRLFQEGRADAIKLEGAGDVLQTIERLTQVGMPCVAHLGLTPQSVGVLEGYKVQGKSLEAA
ETLLQDSLAAERAGAKMLVLECVPHQLAKRIAEELTIPVIGIGAGADVDGQVLVYHDVLKYGVDRLPKFVQAYADLNEVA
TDAIRSYVEETKNGHFPQERHTFTMDESLLDSLYGGK
>Mature_277_residues
MHTMTTLLKKMNEQEKLVMLTAYDYPSAKLAESAGVDLILVGDSLGMVVLGYDSTIPVTMEDMLHHSKAVRRGAQHTFVV
VDMPFASYHGDFDRTLQAASRLFQEGRADAIKLEGAGDVLQTIERLTQVGMPCVAHLGLTPQSVGVLEGYKVQGKSLEAA
ETLLQDSLAAERAGAKMLVLECVPHQLAKRIAEELTIPVIGIGAGADVDGQVLVYHDVLKYGVDRLPKFVQAYADLNEVA
TDAIRSYVEETKNGHFPQERHTFTMDESLLDSLYGGK

Specific function: Catalyzes the reversible reaction in which hydroxymethyl group from 5,10-methylenetetrahydrofolate is tranferred onto alpha-ketoisovalerate to form ketopantoate

COG id: COG0413

COG function: function code H; Ketopantoate hydroxymethyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the panB family

Homologues:

Organism=Escherichia coli, GI1786326, Length=261, Percent_Identity=45.5938697318008, Blast_Score=234, Evalue=4e-63,
Organism=Saccharomyces cerevisiae, GI6319653, Length=265, Percent_Identity=37.7358490566038, Blast_Score=191, Evalue=8e-50,

Paralogues:

None

Copy number: 1080 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): PANB_EXISA (C4L6Q0)

Other databases:

- EMBL:   CP001615
- RefSeq:   YP_002887374.1
- GeneID:   7868619
- GenomeReviews:   CP001615_GR
- KEGG:   eat:EAT1b_3015
- OMA:   ERAGAKM
- ProtClustDB:   PRK00311
- GO:   GO:0005737
- HAMAP:   MF_00156
- InterPro:   IPR003700
- InterPro:   IPR015813
- Gene3D:   G3DSA:3.20.20.60
- PANTHER:   PTHR20881
- PIRSF:   PIRSF000388
- TIGRFAMs:   TIGR00222

Pfam domain/function: PF02548 Pantoate_transf; SSF51621 Pyrv/PenolPyrv_Kinase_cat

EC number: =2.1.2.11

Molecular weight: Translated: 30247; Mature: 30247

Theoretical pI: Translated: 5.03; Mature: 5.03

Prosite motif: NA

Important sites: ACT_SITE 181-181 BINDING 82-82 BINDING 112-112

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHTMTTLLKKMNEQEKLVMLTAYDYPSAKLAESAGVDLILVGDSLGMVVLGYDSTIPVTM
CCHHHHHHHHCCCCCEEEEEEECCCCCHHHHHHCCCEEEEECCCCCEEEEECCCCCCEEH
EDMLHHSKAVRRGAQHTFVVVDMPFASYHGDFDRTLQAASRLFQEGRADAIKLEGAGDVL
HHHHHHHHHHHCCCCCEEEEEECCHHHHCCCHHHHHHHHHHHHHCCCCCEEEECCCCHHH
QTIERLTQVGMPCVAHLGLTPQSVGVLEGYKVQGKSLEAAETLLQDSLAAERAGAKMLVL
HHHHHHHHHCCHHHHHCCCCCCCCCCCCCCEECCCCHHHHHHHHHHHHHHHHCCCEEEHH
ECVPHQLAKRIAEELTIPVIGIGAGADVDGQVLVYHDVLKYGVDRLPKFVQAYADLNEVA
HHHHHHHHHHHHHHCCCCEEEECCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHH
TDAIRSYVEETKNGHFPQERHTFTMDESLLDSLYGGK
HHHHHHHHHHHCCCCCCCCCCCEEHHHHHHHHHCCCC
>Mature Secondary Structure
MHTMTTLLKKMNEQEKLVMLTAYDYPSAKLAESAGVDLILVGDSLGMVVLGYDSTIPVTM
CCHHHHHHHHCCCCCEEEEEEECCCCCHHHHHHCCCEEEEECCCCCEEEEECCCCCCEEH
EDMLHHSKAVRRGAQHTFVVVDMPFASYHGDFDRTLQAASRLFQEGRADAIKLEGAGDVL
HHHHHHHHHHHCCCCCEEEEEECCHHHHCCCHHHHHHHHHHHHHCCCCCEEEECCCCHHH
QTIERLTQVGMPCVAHLGLTPQSVGVLEGYKVQGKSLEAAETLLQDSLAAERAGAKMLVL
HHHHHHHHHCCHHHHHCCCCCCCCCCCCCCEECCCCHHHHHHHHHHHHHHHHCCCEEEHH
ECVPHQLAKRIAEELTIPVIGIGAGADVDGQVLVYHDVLKYGVDRLPKFVQAYADLNEVA
HHHHHHHHHHHHHHCCCCEEEECCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHH
TDAIRSYVEETKNGHFPQERHTFTMDESLLDSLYGGK
HHHHHHHHHHHCCCCCCCCCCCEEHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA