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The map label for this gene is ubiE [H]

Identifier: 229918707

GI number: 229918707

Start: 2955803

End: 2956504

Strand: Direct

Name: ubiE [H]

Synonym: EAT1b_2994

Alternate gene names: 229918707

Gene position: 2955803-2956504 (Clockwise)

Preceding gene: 229918706

Following gene: 229918708

Centisome position: 98.53

GC content: 47.15

Gene sequence:

>702_bases
TTGCAACCGATAGAAAAAGAGCAAAAAGTATACGAAGTATTTCAGTCCATTTCTCCGAGCTACGATACGATGAACTCGAT
TATCAGCTTCCGACTTCATAAATGGTGGCGACGGACAACGATGAAAAAAATGAACGTCTTTCCGGGGGCTAAATGTTTAG
ACGTTTGTTGCGGGACGGCTGACTGGACGATTCAATTGGCAGATGCTGCTGGAAACACGGGTGTTGTGAAAGGACTCGAT
TTTTCAGAGAACATGCTAAAAGTGGGAGAGGAAAAGGTCAAATCTTGGCCGAACATCGAATTGCTGCATGGAAATGCGAT
GGCTCTTCCGTTTGGTGACAACTCCTTTGACTATGTCACAATCGGGTTTGGACTCCGAAACGTTCCAGACTACATGACGG
TTTTGCGTGAAATGCACCGCGTCTTGAAACCGGGTGGAACGGTCGTTTGTCTTGAAACGAGCCAACCGACAGCACCGCTC
TTTAAAGAGTTGTATCGTTTTTACTTTGGAACGGTCATGCCGCTTTTCGGTAAGGTATTTGCCGGTTCATACGACCAATA
TAAGTGGTTGCAGGAGTCGACCGATCAGTTCCCAGACCGTTTGACGCTCAAGCGGATGTTCGAAGATGCCGGGTTTGAAC
AAGTCGAAGTGAAGACATTCTCCGGAGGTGCATGTGCCATGCATCTAGGAAAGAAGTGGTGA

Upstream 100 bases:

>100_bases
TGATTGAAAAGAAAGCGACAGCGCTTAGACAAGACCAAAAAGAGCAACTTACGACGTTGCTCGCGCATGCGGTCGCTTAG
AACGGAAAGGATGTTACGCG

Downstream 100 bases:

>100_bases
ACCATGTCTTTGAAGCGACTTTATCGTCATATGAACCGAGAGATTGATCAAATCGATCAATTCTTATCTACTCGAGTCAG
GACGGACCACCCTGTCATTG

Product: ubiquinone/menaquinone biosynthesis methyltransferase

Products: S-Adenosyl-L-Homocysteine; @OCTA02.txt*2- Polyprenyl-3-Methyl-6-Methoxy-1,4-Benzoquinol!; Menaquinol. [C]

Alternate protein names: NA

Number of amino acids: Translated: 233; Mature: 233

Protein sequence:

>233_residues
MQPIEKEQKVYEVFQSISPSYDTMNSIISFRLHKWWRRTTMKKMNVFPGAKCLDVCCGTADWTIQLADAAGNTGVVKGLD
FSENMLKVGEEKVKSWPNIELLHGNAMALPFGDNSFDYVTIGFGLRNVPDYMTVLREMHRVLKPGGTVVCLETSQPTAPL
FKELYRFYFGTVMPLFGKVFAGSYDQYKWLQESTDQFPDRLTLKRMFEDAGFEQVEVKTFSGGACAMHLGKKW

Sequences:

>Translated_233_residues
MQPIEKEQKVYEVFQSISPSYDTMNSIISFRLHKWWRRTTMKKMNVFPGAKCLDVCCGTADWTIQLADAAGNTGVVKGLD
FSENMLKVGEEKVKSWPNIELLHGNAMALPFGDNSFDYVTIGFGLRNVPDYMTVLREMHRVLKPGGTVVCLETSQPTAPL
FKELYRFYFGTVMPLFGKVFAGSYDQYKWLQESTDQFPDRLTLKRMFEDAGFEQVEVKTFSGGACAMHLGKKW
>Mature_233_residues
MQPIEKEQKVYEVFQSISPSYDTMNSIISFRLHKWWRRTTMKKMNVFPGAKCLDVCCGTADWTIQLADAAGNTGVVKGLD
FSENMLKVGEEKVKSWPNIELLHGNAMALPFGDNSFDYVTIGFGLRNVPDYMTVLREMHRVLKPGGTVVCLETSQPTAPL
FKELYRFYFGTVMPLFGKVFAGSYDQYKWLQESTDQFPDRLTLKRMFEDAGFEQVEVKTFSGGACAMHLGKKW

Specific function: Methyltransferase required for the conversion of dimethylmenaquinone (DMKH2) to menaquinone (MKH2) [H]

COG id: COG2226

COG function: function code H; Methylase involved in ubiquinone/menaquinone biosynthesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. UbiE family [H]

Homologues:

Organism=Homo sapiens, GI116063536, Length=265, Percent_Identity=36.2264150943396, Blast_Score=166, Evalue=1e-41,
Organism=Escherichia coli, GI48994983, Length=238, Percent_Identity=42.8571428571429, Blast_Score=195, Evalue=2e-51,
Organism=Caenorhabditis elegans, GI17557039, Length=233, Percent_Identity=34.3347639484979, Blast_Score=144, Evalue=4e-35,
Organism=Saccharomyces cerevisiae, GI6323526, Length=245, Percent_Identity=33.8775510204082, Blast_Score=130, Evalue=1e-31,
Organism=Drosophila melanogaster, GI24641702, Length=244, Percent_Identity=36.0655737704918, Blast_Score=149, Evalue=1e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004033 [H]

Pfam domain/function: PF01209 Ubie_methyltran [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 26564; Mature: 26564

Theoretical pI: Translated: 7.87; Mature: 7.87

Prosite motif: PS01183 UBIE_1 ; PS01184 UBIE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
6.9 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
4.7 %Met     (Mature Protein)
6.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQPIEKEQKVYEVFQSISPSYDTMNSIISFRLHKWWRRTTMKKMNVFPGAKCLDVCCGTA
CCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCC
DWTIQLADAAGNTGVVKGLDFSENMLKVGEEKVKSWPNIELLHGNAMALPFGDNSFDYVT
CCEEEEECCCCCCCEEECCCCHHHHHHHHHHHHHCCCCEEEEECCEEEEECCCCCCCEEE
IGFGLRNVPDYMTVLREMHRVLKPGGTVVCLETSQPTAPLFKELYRFYFGTVMPLFGKVF
ECCCCCCCCHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHH
AGSYDQYKWLQESTDQFPDRLTLKRMFEDAGFEQVEVKTFSGGACAMHLGKKW
CCCCHHHHHHHHHHHHCCHHHHHHHHHHHCCCCEEEEEEECCCEEEECCCCCC
>Mature Secondary Structure
MQPIEKEQKVYEVFQSISPSYDTMNSIISFRLHKWWRRTTMKKMNVFPGAKCLDVCCGTA
CCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCC
DWTIQLADAAGNTGVVKGLDFSENMLKVGEEKVKSWPNIELLHGNAMALPFGDNSFDYVT
CCEEEEECCCCCCCEEECCCCHHHHHHHHHHHHHCCCCEEEEECCEEEEECCCCCCCEEE
IGFGLRNVPDYMTVLREMHRVLKPGGTVVCLETSQPTAPLFKELYRFYFGTVMPLFGKVF
ECCCCCCCCHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHH
AGSYDQYKWLQESTDQFPDRLTLKRMFEDAGFEQVEVKTFSGGACAMHLGKKW
CCCCHHHHHHHHHHHHCCHHHHHHHHHHHCCCCEEEEEEECCCEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: S-Adenosyl-L-Methionine; 2-Polyprenyl-6- Methoxy-1,4-Benzoquinol; Demethylmenaquinol [C]

Specific reaction: S-Adenosyl-L-Methionine + @OCTA02.txt*2-Polyprenyl-6- Methoxy-1,4-Benzoquinol! = S-Adenosyl-L-Homocysteine + 2- Polyprenyl-3-Methyl-6-Methoxy -1,4-Benzoquinol S-Adenosyl-L-Methionine + Demethylmenaquinol = S-Adenosyl-L-Homocysteine + Menaquinol. [C]

General reaction: Transferring one-carbon groups; Methyltransferases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA