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The map label for this gene is gudB [H]

Identifier: 229918693

GI number: 229918693

Start: 2943770

End: 2945038

Strand: Direct

Name: gudB [H]

Synonym: EAT1b_2980

Alternate gene names: 229918693

Gene position: 2943770-2945038 (Clockwise)

Preceding gene: 229918692

Following gene: 229918696

Centisome position: 98.13

GC content: 48.38

Gene sequence:

>1269_bases
ATGATTACGGATCAAGAAAAAGGTCATCAAAAACGAAAAAACATCTTGGAATCGACACAAGAGGTAATTCACGAAGCTTT
AGATAAACTCGGCTATCCAGATGAGATGTACGAGTTATTAAAAGAACCGCTTCGCATGCTGACCGTTCGGATCCCTGTTC
GCATGGACGATGGCTCAACGAAGATCTTTACAGGCTATCGCGCTCAACACAATGATGCGGTCGGACCGACTAAAGGGGGA
ATTCGGTTCCACCCGAGCGTAACGGAGGTAGAAGTCAAAGCATTATCAGTATGGATGAGTTTAAAAGCTGGAATCGTCGA
CTTGCCTTACGGTGGTGGGAAAGGCGGAATCGTCTGTGACCCACGCGAGATGAGTTTCCGTGAAATCGAACGTTTAAGTC
GCGGATACGTCCGGGCCATCAGTCAAATTGTTGGGCCGACGAAAGATATCCCAGCTCCGGACGTGTTCACAAACTCACAG
ATCATGGCATGGATGATGGATGAATACAGTCGCATCGATGAATTCAACTCACCTGGATTCATCACAGGAAAACCGCTCGT
TCTTGGAGGGTCGCATGGTCGTGAGACAGCGACGGCAAAAGGCGTGGCCATCATGATTCGTGAAGCGGCACTCCGTCGCG
GAATCGAATTGAAAGGTGCACGGGTCGTCGTCCAAGGATTCGGAAACGCGGGAAGTTTCCTTTCTAAATTCATGTACGAT
GCAGGGGCGAAAGTCATTGCAGTCAGTGACGCGTATGGAGCCATCCATGATGAGAACGGTCTCGACATCCCTTACTTGTT
AGATCGTCGCGATTCATTCGGTACGATTTCGACATTGTTTAAAAATACGATCTCAAACAAAGAAATGTTAGAGCTCGATT
GTGATATTTTGGTTCCGGCTGCGATCGAGAACCAAATTACAGAGGACAATGCGGATGCGATCAAAGCATCGATCGTGGTC
GAGGCGGCCAACGGTCCAACGACGAACGAAGCAACGAAAATTTTGACTGAACGTGGAATTCTCCTCGTGCCTGACGTGTT
GGCGTCAAGTGGTGGGGTTACGGTGTCATACTTCGAATGGGTACAAAACAACCAAGGTTACTATTGGACGGAAGAAGAAG
TTGAGGAGAAACTTGAAAAAGTACTCGTTCACTCGTTCAATACGGTATACAATACATCTTCAGCCCGTAAAGTCGATATG
CGTCTCGCCGCCTACATGGTCGGTGTACGCAAAATGGCGGAAGCCTCACGTTTCCGTGGGTGGGTCTAA

Upstream 100 bases:

>100_bases
TGAAAAGCCCTTTCAAATTGAACGCGTATCAGCTATACTTTTAAACGAATACGAGGTTAATTCTTGGGGGAGAATTGACA
ATTTAGGGGAGGAAACCTTC

Downstream 100 bases:

>100_bases
TCAAAAAAAGTTCGGTCAACTCAAGTTGACCGAACTTTTTTATACGTTAATGCGGTTCGCAATCGCATCGGCAATCGCCT
TGCCGTGGAATCTACCGTTC

Product: Glu/Leu/Phe/Val dehydrogenase

Products: NA

Alternate protein names: NAD-GDH [H]

Number of amino acids: Translated: 422; Mature: 422

Protein sequence:

>422_residues
MITDQEKGHQKRKNILESTQEVIHEALDKLGYPDEMYELLKEPLRMLTVRIPVRMDDGSTKIFTGYRAQHNDAVGPTKGG
IRFHPSVTEVEVKALSVWMSLKAGIVDLPYGGGKGGIVCDPREMSFREIERLSRGYVRAISQIVGPTKDIPAPDVFTNSQ
IMAWMMDEYSRIDEFNSPGFITGKPLVLGGSHGRETATAKGVAIMIREAALRRGIELKGARVVVQGFGNAGSFLSKFMYD
AGAKVIAVSDAYGAIHDENGLDIPYLLDRRDSFGTISTLFKNTISNKEMLELDCDILVPAAIENQITEDNADAIKASIVV
EAANGPTTNEATKILTERGILLVPDVLASSGGVTVSYFEWVQNNQGYYWTEEEVEEKLEKVLVHSFNTVYNTSSARKVDM
RLAAYMVGVRKMAEASRFRGWV

Sequences:

>Translated_422_residues
MITDQEKGHQKRKNILESTQEVIHEALDKLGYPDEMYELLKEPLRMLTVRIPVRMDDGSTKIFTGYRAQHNDAVGPTKGG
IRFHPSVTEVEVKALSVWMSLKAGIVDLPYGGGKGGIVCDPREMSFREIERLSRGYVRAISQIVGPTKDIPAPDVFTNSQ
IMAWMMDEYSRIDEFNSPGFITGKPLVLGGSHGRETATAKGVAIMIREAALRRGIELKGARVVVQGFGNAGSFLSKFMYD
AGAKVIAVSDAYGAIHDENGLDIPYLLDRRDSFGTISTLFKNTISNKEMLELDCDILVPAAIENQITEDNADAIKASIVV
EAANGPTTNEATKILTERGILLVPDVLASSGGVTVSYFEWVQNNQGYYWTEEEVEEKLEKVLVHSFNTVYNTSSARKVDM
RLAAYMVGVRKMAEASRFRGWV
>Mature_422_residues
MITDQEKGHQKRKNILESTQEVIHEALDKLGYPDEMYELLKEPLRMLTVRIPVRMDDGSTKIFTGYRAQHNDAVGPTKGG
IRFHPSVTEVEVKALSVWMSLKAGIVDLPYGGGKGGIVCDPREMSFREIERLSRGYVRAISQIVGPTKDIPAPDVFTNSQ
IMAWMMDEYSRIDEFNSPGFITGKPLVLGGSHGRETATAKGVAIMIREAALRRGIELKGARVVVQGFGNAGSFLSKFMYD
AGAKVIAVSDAYGAIHDENGLDIPYLLDRRDSFGTISTLFKNTISNKEMLELDCDILVPAAIENQITEDNADAIKASIVV
EAANGPTTNEATKILTERGILLVPDVLASSGGVTVSYFEWVQNNQGYYWTEEEVEEKLEKVLVHSFNTVYNTSSARKVDM
RLAAYMVGVRKMAEASRFRGWV

Specific function: The wild-type version has little or no activity [H]

COG id: COG0334

COG function: function code E; Glutamate dehydrogenase/leucine dehydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Glu/Leu/Phe/Val dehydrogenases family [H]

Homologues:

Organism=Homo sapiens, GI31377775, Length=338, Percent_Identity=41.4201183431953, Blast_Score=261, Evalue=1e-69,
Organism=Homo sapiens, GI4885281, Length=338, Percent_Identity=40.8284023668639, Blast_Score=257, Evalue=2e-68,
Organism=Escherichia coli, GI1788059, Length=401, Percent_Identity=31.4214463840399, Blast_Score=172, Evalue=4e-44,
Organism=Caenorhabditis elegans, GI17544676, Length=338, Percent_Identity=40.5325443786982, Blast_Score=250, Evalue=8e-67,
Organism=Saccharomyces cerevisiae, GI6319256, Length=417, Percent_Identity=29.2565947242206, Blast_Score=149, Evalue=1e-36,
Organism=Saccharomyces cerevisiae, GI6324951, Length=395, Percent_Identity=28.3544303797468, Blast_Score=141, Evalue=2e-34,
Organism=Drosophila melanogaster, GI45553475, Length=371, Percent_Identity=38.544474393531, Blast_Score=253, Evalue=2e-67,
Organism=Drosophila melanogaster, GI45549226, Length=340, Percent_Identity=40.2941176470588, Blast_Score=253, Evalue=2e-67,
Organism=Drosophila melanogaster, GI24649283, Length=328, Percent_Identity=39.9390243902439, Blast_Score=250, Evalue=1e-66,
Organism=Drosophila melanogaster, GI24649460, Length=279, Percent_Identity=39.0681003584229, Blast_Score=200, Evalue=2e-51,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 9362 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006095
- InterPro:   IPR006096
- InterPro:   IPR006097
- InterPro:   IPR014362
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00208 ELFV_dehydrog; PF02812 ELFV_dehydrog_N [H]

EC number: =1.4.1.2 [H]

Molecular weight: Translated: 46670; Mature: 46670

Theoretical pI: Translated: 5.54; Mature: 5.54

Prosite motif: PS00074 GLFV_DEHYDROGENASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MITDQEKGHQKRKNILESTQEVIHEALDKLGYPDEMYELLKEPLRMLTVRIPVRMDDGST
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHEEEEEEEEECCCCE
KIFTGYRAQHNDAVGPTKGGIRFHPSVTEVEVKALSVWMSLKAGIVDLPYGGGKGGIVCD
EEEEEECCCCCCCCCCCCCCEEECCCHHHHHHHHHHHHHHHHCCEEECCCCCCCCCEEEC
PREMSFREIERLSRGYVRAISQIVGPTKDIPAPDVFTNSQIMAWMMDEYSRIDEFNSPGF
CCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCE
ITGKPLVLGGSHGRETATAKGVAIMIREAALRRGIELKGARVVVQGFGNAGSFLSKFMYD
ECCCCEEEECCCCCCCHHHCCHHHHHHHHHHHCCCCCCCCEEEEEECCCHHHHHHHHHHH
AGAKVIAVSDAYGAIHDENGLDIPYLLDRRDSFGTISTLFKNTISNKEMLELDCDILVPA
CCCEEEEEECCCCCEECCCCCCCCCEECCCCCCHHHHHHHHHHCCCCEEEEECCCEEECH
AIENQITEDNADAIKASIVVEAANGPTTNEATKILTERGILLVPDVLASSGGVTVSYFEW
HHHHCCCCCCHHHEEEEEEEECCCCCCCHHHHHHHHHCCCEEECHHHHCCCCCEEEEEHH
VQNNQGYYWTEEEVEEKLEKVLVHSFNTVYNTSSARKVDMRLAAYMVGVRKMAEASRFRG
HHCCCCEEECHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCC
WV
CC
>Mature Secondary Structure
MITDQEKGHQKRKNILESTQEVIHEALDKLGYPDEMYELLKEPLRMLTVRIPVRMDDGST
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHEEEEEEEEECCCCE
KIFTGYRAQHNDAVGPTKGGIRFHPSVTEVEVKALSVWMSLKAGIVDLPYGGGKGGIVCD
EEEEEECCCCCCCCCCCCCCEEECCCHHHHHHHHHHHHHHHHCCEEECCCCCCCCCEEEC
PREMSFREIERLSRGYVRAISQIVGPTKDIPAPDVFTNSQIMAWMMDEYSRIDEFNSPGF
CCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCE
ITGKPLVLGGSHGRETATAKGVAIMIREAALRRGIELKGARVVVQGFGNAGSFLSKFMYD
ECCCCEEEECCCCCCCHHHCCHHHHHHHHHHHCCCCCCCCEEEEEECCCHHHHHHHHHHH
AGAKVIAVSDAYGAIHDENGLDIPYLLDRRDSFGTISTLFKNTISNKEMLELDCDILVPA
CCCEEEEEECCCCCEECCCCCCCCCEECCCCCCHHHHHHHHHHCCCCEEEEECCCEEECH
AIENQITEDNADAIKASIVVEAANGPTTNEATKILTERGILLVPDVLASSGGVTVSYFEW
HHHHCCCCCCHHHEEEEEEEECCCCCCCHHHHHHHHHCCCEEECHHHHCCCCCEEEEEHH
VQNNQGYYWTEEEVEEKLEKVLVHSFNTVYNTSSARKVDMRLAAYMVGVRKMAEASRFRG
HHCCCCEEECHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCC
WV
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8760912; 9384377; 9829940 [H]