Click here to switch to the map view.

The map label for this gene is ddl [H]

Identifier: 229918692

GI number: 229918692

Start: 2942697

End: 2943623

Strand: Direct

Name: ddl [H]

Synonym: EAT1b_2979

Alternate gene names: 229918692

Gene position: 2942697-2943623 (Clockwise)

Preceding gene: 229918691

Following gene: 229918693

Centisome position: 98.09

GC content: 51.13

Gene sequence:

>927_bases
ATGAAAGTCGCTGTTTTATATGGTGGAGTGTCAGGAGAACGAGAGGTATCCCTCACGAGCGGGCAAGGAATGATAGAAGC
GTTGACGGAACGTGGTCATGAGGTCACGGCCATTGACTTTCATCCGGAACGTGCGAACGAGTTATTAACGCTGGACGTCG
ACGTCGTCGTCAGTGCGTTGCACGGGAAGTATGGTGAAGATGGTCGTGTGCAATCGTTATTAGAAATGGCTGGGATTCCT
TACACGGGATCAGGTGTCTTGGCATCTGCACTGGCGATGGACAAGGCGAGAGCGAAGATCATCTTCGCGGCATCCGGGAT
CCGCGTCGCGCGTGACGTCTTAATCGAGTCACGGGAATCGATCGATGCAAAAGTCGCTGAGTGGGGAATGGAATTCCCAT
GTGTCGTCAAACCAGCGCAGGAAGGATCGTCGAACGGTCTGACGATTGCCATGGACGAAGCGATGTTACGTGAAGGAATT
GAAAAGGCGTTTGCGTGTGATTCAGCGGTACTTGTTGAACAGTACATCAAAGGGAAAGAGCTGACAGTTCCCGTCATCGG
GAACTTCGGGAAAGAAGAGGCTCTTCCTGTCATTGAAATCATTCCAAAAAATGAATTTTACGACTATGAATCGAAGTATA
CGGAAGGTGGTTCGATTCATGTCTGTCCGGCTGAAATCTCGGACGATTTGACATCAGAGGTTCAACAGGCGGCAGTCCTG
GCACATCGCGCGCTCGGTTGTTCAGGATATTCGCGCTCTGACTTCCTCGTTTCAGAAGAAGGCAAAGCCTACATTCTAGA
GACGAATACGCTACCGGGCATGACGCCGCTTAGCCTGTTTCCGGATAGTGCCAAGACAGTCGGAATCTCGTACGGTGAGC
TATTGGAACGCTTTATCGAGCTCGCACTTGAGCGCGTAGCGAAGTAA

Upstream 100 bases:

>100_bases
TGAGTACGGCTTTTTTTACTTTTCTGTTCCTATTGTTTTTAGGATGTCCGTGTTATGATTAGACTTGAATTTTCAGTAGT
TAGTTGAAGGAGAGATTGGA

Downstream 100 bases:

>100_bases
GACATCTGGATCACGTCATCCATCAAGAAAACGCTTTCATTATTTTTGAAAAGCCCTTTCAAATTGAACGCGTATCAGCT
ATACTTTTAAACGAATACGA

Product: D-alanine/D-alanine ligase

Products: NA

Alternate protein names: D-Ala-D-Ala ligase; D-alanylalanine synthetase [H]

Number of amino acids: Translated: 308; Mature: 308

Protein sequence:

>308_residues
MKVAVLYGGVSGEREVSLTSGQGMIEALTERGHEVTAIDFHPERANELLTLDVDVVVSALHGKYGEDGRVQSLLEMAGIP
YTGSGVLASALAMDKARAKIIFAASGIRVARDVLIESRESIDAKVAEWGMEFPCVVKPAQEGSSNGLTIAMDEAMLREGI
EKAFACDSAVLVEQYIKGKELTVPVIGNFGKEEALPVIEIIPKNEFYDYESKYTEGGSIHVCPAEISDDLTSEVQQAAVL
AHRALGCSGYSRSDFLVSEEGKAYILETNTLPGMTPLSLFPDSAKTVGISYGELLERFIELALERVAK

Sequences:

>Translated_308_residues
MKVAVLYGGVSGEREVSLTSGQGMIEALTERGHEVTAIDFHPERANELLTLDVDVVVSALHGKYGEDGRVQSLLEMAGIP
YTGSGVLASALAMDKARAKIIFAASGIRVARDVLIESRESIDAKVAEWGMEFPCVVKPAQEGSSNGLTIAMDEAMLREGI
EKAFACDSAVLVEQYIKGKELTVPVIGNFGKEEALPVIEIIPKNEFYDYESKYTEGGSIHVCPAEISDDLTSEVQQAAVL
AHRALGCSGYSRSDFLVSEEGKAYILETNTLPGMTPLSLFPDSAKTVGISYGELLERFIELALERVAK
>Mature_308_residues
MKVAVLYGGVSGEREVSLTSGQGMIEALTERGHEVTAIDFHPERANELLTLDVDVVVSALHGKYGEDGRVQSLLEMAGIP
YTGSGVLASALAMDKARAKIIFAASGIRVARDVLIESRESIDAKVAEWGMEFPCVVKPAQEGSSNGLTIAMDEAMLREGI
EKAFACDSAVLVEQYIKGKELTVPVIGNFGKEEALPVIEIIPKNEFYDYESKYTEGGSIHVCPAEISDDLTSEVQQAAVL
AHRALGCSGYSRSDFLVSEEGKAYILETNTLPGMTPLSLFPDSAKTVGISYGELLERFIELALERVAK

Specific function: Cell wall formation [H]

COG id: COG1181

COG function: function code M; D-alanine-D-alanine ligase and related ATP-grasp enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Escherichia coli, GI1786280, Length=306, Percent_Identity=40.8496732026144, Blast_Score=229, Evalue=2e-61,
Organism=Escherichia coli, GI1786579, Length=353, Percent_Identity=32.8611898016997, Blast_Score=182, Evalue=2e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR000291
- InterPro:   IPR005905
- InterPro:   IPR011095
- InterPro:   IPR011127
- InterPro:   IPR013817
- InterPro:   IPR016185 [H]

Pfam domain/function: PF07478 Dala_Dala_lig_C; PF01820 Dala_Dala_lig_N [H]

EC number: =6.3.2.4 [H]

Molecular weight: Translated: 33168; Mature: 33168

Theoretical pI: Translated: 4.39; Mature: 4.39

Prosite motif: PS50975 ATP_GRASP ; PS00843 DALA_DALA_LIGASE_1 ; PS00844 DALA_DALA_LIGASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVAVLYGGVSGEREVSLTSGQGMIEALTERGHEVTAIDFHPERANELLTLDVDVVVSAL
CEEEEEECCCCCCEEEEEECCCHHHHHHHHCCCEEEEEEECHHCCCCEEEEEHHHHHHHH
HGKYGEDGRVQSLLEMAGIPYTGSGVLASALAMDKARAKIIFAASGIRVARDVLIESRES
CCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCEEEEEEECCHHHHHHHHHHHHHH
IDAKVAEWGMEFPCVVKPAQEGSSNGLTIAMDEAMLREGIEKAFACDSAVLVEQYIKGKE
HHHHHHHCCCCCCEEEECCCCCCCCCEEEEECHHHHHHHHHHHHHCCHHHHHHHHHCCCC
LTVPVIGNFGKEEALPVIEIIPKNEFYDYESKYTEGGSIHVCPAEISDDLTSEVQQAAVL
EEEEEECCCCCCCCCCEEEEECCCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHH
AHRALGCSGYSRSDFLVSEEGKAYILETNTLPGMTPLSLFPDSAKTVGISYGELLERFIE
HHHHHCCCCCCCCCEEEECCCCEEEEECCCCCCCCCHHCCCCCCCEECCCHHHHHHHHHH
LALERVAK
HHHHHHCC
>Mature Secondary Structure
MKVAVLYGGVSGEREVSLTSGQGMIEALTERGHEVTAIDFHPERANELLTLDVDVVVSAL
CEEEEEECCCCCCEEEEEECCCHHHHHHHHCCCEEEEEEECHHCCCCEEEEEHHHHHHHH
HGKYGEDGRVQSLLEMAGIPYTGSGVLASALAMDKARAKIIFAASGIRVARDVLIESRES
CCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCEEEEEEECCHHHHHHHHHHHHHH
IDAKVAEWGMEFPCVVKPAQEGSSNGLTIAMDEAMLREGIEKAFACDSAVLVEQYIKGKE
HHHHHHHCCCCCCEEEECCCCCCCCCEEEEECHHHHHHHHHHHHHCCHHHHHHHHHCCCC
LTVPVIGNFGKEEALPVIEIIPKNEFYDYESKYTEGGSIHVCPAEISDDLTSEVQQAAVL
EEEEEECCCCCCCCCCEEEEECCCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHH
AHRALGCSGYSRSDFLVSEEGKAYILETNTLPGMTPLSLFPDSAKTVGISYGELLERFIE
HHHHHCCCCCCCCCEEEECCCCEEEEECCCCCCCCCHHCCCCCCCEECCCHHHHHHHHHH
LALERVAK
HHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11058132 [H]