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The map label for this gene is rnjB [H]

Identifier: 229918679

GI number: 229918679

Start: 2927876

End: 2929543

Strand: Direct

Name: rnjB [H]

Synonym: EAT1b_2966

Alternate gene names: 229918679

Gene position: 2927876-2929543 (Clockwise)

Preceding gene: 229918678

Following gene: 229918680

Centisome position: 97.6

GC content: 48.14

Gene sequence:

>1668_bases
GTGTCAAAAAAGAAGACAGAGAATATTAGCGTCTTTGCGCTTGGAGGCGTAGGCGAAATCGGGAAGAACATGTACGTCGT
GGAACTAGACGACGACATCTTCGTCATCGATGCCGGTCTAATGTTTCCAGGCGATGAGATGCTCGGGATTGATATCGTCA
TCCCAGACATCACATACTTAAAAGAAAATAAAGATCGCGTACGGGCGATTTTCATCACCCATGGTCACGAAGACCATATC
GGTGGACTCAGCTACGTCTTACGCGACTTGAAGCACGTTCCAGTATACGGGACGAAATTGACCCTCGGGTTGATTGAATT
GAAATTAAAAGAGGCCGGTTTGTTGAAAGAAGTCGACCTTCGCATGATTGATGCCAAAACGAAATTGACTATCGCTGATC
ATTTCATCACGTTTTTCCGCGTCAATCACTCGATTCCCGACTGTGTCGGCGTCTGTATTCAAACGTCTCAAGGTGCGATC
GTCCATACCGGAGATTTCAAGTTTGATTACACGCCTGTCGATGGTAAACAATCCGACTTCAATAAGTTAGCGGCAATCGG
ACAACGTGGTGTACTTGCACTTCTTTCCGACTCGACGAACGCCGAGCGACCGGGGCAAACCGGTTCAGAGTCAATCGTCG
GAGCTGAATTGAACGATGTCATTTATCAAGCGGAAGGTCGGGTTATCGTGGCATCATTCGCCTCGAACGTCCATCGCTTG
CAACAAGTATTCGCAGCAGCGGAAGCGAATGATCGTAAAGTCGCGGTCGTTGGTCGTAGCATGGTCAACATCGTCGAAGT
TGCACAGAAACTAAACTACTTACGTTTCAAGGCGAAGACACTCGTCGAGTTGTCGCAAGTAAACCGTCTACCGGATAATA
AAGTCGTCATTTTAACGACAGGTTCGCAAGGAGAGCCGATGGCGGCATTGACTCGTATGGCTCGTAATGCCCACAAGCAA
GTCAACATTCGATTGAATGACACGGTCGTCATCGCGGCGACACCGATTCCAGGGAATGAGAAGTCCGTTTCTAAAACGAT
TGATTTGTTGTTCCGTTCGGGTGCGAACGTCATTTACAACCAACGTAAAGTGCACGTATCTGGACACGGCTCGGCAGAAG
AATTGAAGCTGATGCTCAACCTCGTTAAACCGAAGTACTTCTTACCGATTCACGGGGAATACCGGATGCAGATGGCACAC
TCGAAACTCGCTGAACAAGTGGGCGTCAAACCAAACAACATCTTCATCGTTGAAAAAGGTGACGTCGTCGAGTTCACGGG
TCGAAAAGCCCGCATGAGCCGAAAAGTTCCTGCAGGAAACGTCTTGATTGACGGAATCGGCGTCGGGGACGTTGGAAACA
TCGTCTTACGGGACCGTCGTTTGTTGTCACAGGATGGAGTTGTCCTTTGCGTGGTCACGTTGAATCGCAAACAGAAGAAA
TTGATTTCAGGACCTGAACTCATCTCACGTGGTTTCGTATATATGCGTGAATCGGAGGAGATGATTTCAGAGGCAAACCG
TATTGTCACGAAACAGATTGAAAAGAGTCTGCAGGCTGGCAGTGATTGGACCGAATTGAAATCAAATATCCGCGAGCGAC
TGAGCGTCTTCTTATTCGAGCAGACGAAACGTCGTCCGATGATCTTGCCGATCATCATGGAGATTTAA

Upstream 100 bases:

>100_bases
ATTAGACGGAAATGTTGGCAATGAAGCCGTTTTCCGCTACAATATTCAGTAGCGATAAGGGGACGAAACAGAATATGAAT
CATTTAGGAGGTTTTTGTTA

Downstream 100 bases:

>100_bases
TGGAATGAGAGTGAGGCAAAAGTCATCTGACTTTTGCCTTCTTTTTTTAGGAGGAACTGATGTGGCTCAAAAAACAAGAA
AGACTCCCGTAAAGCGAAAG

Product: beta-lactamase domain protein

Products: NA

Alternate protein names: RNase J2 [H]

Number of amino acids: Translated: 555; Mature: 554

Protein sequence:

>555_residues
MSKKKTENISVFALGGVGEIGKNMYVVELDDDIFVIDAGLMFPGDEMLGIDIVIPDITYLKENKDRVRAIFITHGHEDHI
GGLSYVLRDLKHVPVYGTKLTLGLIELKLKEAGLLKEVDLRMIDAKTKLTIADHFITFFRVNHSIPDCVGVCIQTSQGAI
VHTGDFKFDYTPVDGKQSDFNKLAAIGQRGVLALLSDSTNAERPGQTGSESIVGAELNDVIYQAEGRVIVASFASNVHRL
QQVFAAAEANDRKVAVVGRSMVNIVEVAQKLNYLRFKAKTLVELSQVNRLPDNKVVILTTGSQGEPMAALTRMARNAHKQ
VNIRLNDTVVIAATPIPGNEKSVSKTIDLLFRSGANVIYNQRKVHVSGHGSAEELKLMLNLVKPKYFLPIHGEYRMQMAH
SKLAEQVGVKPNNIFIVEKGDVVEFTGRKARMSRKVPAGNVLIDGIGVGDVGNIVLRDRRLLSQDGVVLCVVTLNRKQKK
LISGPELISRGFVYMRESEEMISEANRIVTKQIEKSLQAGSDWTELKSNIRERLSVFLFEQTKRRPMILPIIMEI

Sequences:

>Translated_555_residues
MSKKKTENISVFALGGVGEIGKNMYVVELDDDIFVIDAGLMFPGDEMLGIDIVIPDITYLKENKDRVRAIFITHGHEDHI
GGLSYVLRDLKHVPVYGTKLTLGLIELKLKEAGLLKEVDLRMIDAKTKLTIADHFITFFRVNHSIPDCVGVCIQTSQGAI
VHTGDFKFDYTPVDGKQSDFNKLAAIGQRGVLALLSDSTNAERPGQTGSESIVGAELNDVIYQAEGRVIVASFASNVHRL
QQVFAAAEANDRKVAVVGRSMVNIVEVAQKLNYLRFKAKTLVELSQVNRLPDNKVVILTTGSQGEPMAALTRMARNAHKQ
VNIRLNDTVVIAATPIPGNEKSVSKTIDLLFRSGANVIYNQRKVHVSGHGSAEELKLMLNLVKPKYFLPIHGEYRMQMAH
SKLAEQVGVKPNNIFIVEKGDVVEFTGRKARMSRKVPAGNVLIDGIGVGDVGNIVLRDRRLLSQDGVVLCVVTLNRKQKK
LISGPELISRGFVYMRESEEMISEANRIVTKQIEKSLQAGSDWTELKSNIRERLSVFLFEQTKRRPMILPIIMEI
>Mature_554_residues
SKKKTENISVFALGGVGEIGKNMYVVELDDDIFVIDAGLMFPGDEMLGIDIVIPDITYLKENKDRVRAIFITHGHEDHIG
GLSYVLRDLKHVPVYGTKLTLGLIELKLKEAGLLKEVDLRMIDAKTKLTIADHFITFFRVNHSIPDCVGVCIQTSQGAIV
HTGDFKFDYTPVDGKQSDFNKLAAIGQRGVLALLSDSTNAERPGQTGSESIVGAELNDVIYQAEGRVIVASFASNVHRLQ
QVFAAAEANDRKVAVVGRSMVNIVEVAQKLNYLRFKAKTLVELSQVNRLPDNKVVILTTGSQGEPMAALTRMARNAHKQV
NIRLNDTVVIAATPIPGNEKSVSKTIDLLFRSGANVIYNQRKVHVSGHGSAEELKLMLNLVKPKYFLPIHGEYRMQMAHS
KLAEQVGVKPNNIFIVEKGDVVEFTGRKARMSRKVPAGNVLIDGIGVGDVGNIVLRDRRLLSQDGVVLCVVTLNRKQKKL
ISGPELISRGFVYMRESEEMISEANRIVTKQIEKSLQAGSDWTELKSNIRERLSVFLFEQTKRRPMILPIIMEI

Specific function: Cleaves the 5'-leader sequence of certain mRNAs. May play a role in maturation and stability of specific mRNAs. Appears to have a limited effect on 16S rRNA maturation, despite its similarity to rnjA [H]

COG id: COG0595

COG function: function code R; Predicted hydrolase of the metallo-beta-lactamase superfamily

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the metallo-beta-lactamase superfamily. RNA-metabolizing metallo-beta-lactamase-like family. RNase J subfamily [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001279
- InterPro:   IPR011108
- InterPro:   IPR004613 [H]

Pfam domain/function: PF00753 Lactamase_B; PF07521 RMMBL [H]

EC number: NA

Molecular weight: Translated: 61518; Mature: 61387

Theoretical pI: Translated: 9.62; Mature: 9.62

Prosite motif: PS01292 UPF0036

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKKKTENISVFALGGVGEIGKNMYVVELDDDIFVIDAGLMFPGDEMLGIDIVIPDITYL
CCCCCCCCEEEEEECCCHHHCCCEEEEEECCCEEEEEECEECCCCCEEEEEEEECCCCEE
KENKDRVRAIFITHGHEDHIGGLSYVLRDLKHVPVYGTKLTLGLIELKLKEAGLLKEVDL
CCCCCEEEEEEEECCCCCCCCHHHHHHHHHHCCCCCCCEEEEEEEEEEEHHCCCHHHCCE
RMIDAKTKLTIADHFITFFRVNHSIPDCVGVCIQTSQGAIVHTGDFKFDYTPVDGKQSDF
EEECCCEEEEHHHHEEEEEEECCCCCHHHHHEEECCCCCEEECCCCEEEECCCCCCCCHH
NKLAAIGQRGVLALLSDSTNAERPGQTGSESIVGAELNDVIYQAEGRVIVASFASNVHRL
HHHHHHCCCCEEEEEECCCCCCCCCCCCCCCEECCCHHHHEEECCCCEEEEHHHHHHHHH
QQVFAAAEANDRKVAVVGRSMVNIVEVAQKLNYLRFKAKTLVELSQVNRLPDNKVVILTT
HHHHHHHCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEE
GSQGEPMAALTRMARNAHKQVNIRLNDTVVIAATPIPGNEKSVSKTIDLLFRSGANVIYN
CCCCCHHHHHHHHHHCCCCEEEEEECCEEEEEEECCCCCCHHHHHHHHHHHHCCCCEEEE
QRKVHVSGHGSAEELKLMLNLVKPKYFLPIHGEYRMQMAHSKLAEQVGVKPNNIFIVEKG
CEEEEEECCCCHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHHCCCCCCEEEEECC
DVVEFTGRKARMSRKVPAGNVLIDGIGVGDVGNIVLRDRRLLSQDGVVLCVVTLNRKQKK
CEEEECCCHHHHHCCCCCCCEEEEECCCCCCCCEEECCCHHHCCCCEEEEEEECCCCHHH
LISGPELISRGFVYMRESEEMISEANRIVTKQIEKSLQAGSDWTELKSNIRERLSVFLFE
HCCCHHHHHCCEEEEECHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
QTKRRPMILPIIMEI
HCCCCCEEEEEEEEC
>Mature Secondary Structure 
SKKKTENISVFALGGVGEIGKNMYVVELDDDIFVIDAGLMFPGDEMLGIDIVIPDITYL
CCCCCCCEEEEEECCCHHHCCCEEEEEECCCEEEEEECEECCCCCEEEEEEEECCCCEE
KENKDRVRAIFITHGHEDHIGGLSYVLRDLKHVPVYGTKLTLGLIELKLKEAGLLKEVDL
CCCCCEEEEEEEECCCCCCCCHHHHHHHHHHCCCCCCCEEEEEEEEEEEHHCCCHHHCCE
RMIDAKTKLTIADHFITFFRVNHSIPDCVGVCIQTSQGAIVHTGDFKFDYTPVDGKQSDF
EEECCCEEEEHHHHEEEEEEECCCCCHHHHHEEECCCCCEEECCCCEEEECCCCCCCCHH
NKLAAIGQRGVLALLSDSTNAERPGQTGSESIVGAELNDVIYQAEGRVIVASFASNVHRL
HHHHHHCCCCEEEEEECCCCCCCCCCCCCCCEECCCHHHHEEECCCCEEEEHHHHHHHHH
QQVFAAAEANDRKVAVVGRSMVNIVEVAQKLNYLRFKAKTLVELSQVNRLPDNKVVILTT
HHHHHHHCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEE
GSQGEPMAALTRMARNAHKQVNIRLNDTVVIAATPIPGNEKSVSKTIDLLFRSGANVIYN
CCCCCHHHHHHHHHHCCCCEEEEEECCEEEEEEECCCCCCHHHHHHHHHHHHCCCCEEEE
QRKVHVSGHGSAEELKLMLNLVKPKYFLPIHGEYRMQMAHSKLAEQVGVKPNNIFIVEKG
CEEEEEECCCCHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHHCCCCCCEEEEECC
DVVEFTGRKARMSRKVPAGNVLIDGIGVGDVGNIVLRDRRLLSQDGVVLCVVTLNRKQKK
CEEEECCCHHHHHCCCCCCCEEEEECCCCCCCCEEECCCHHHCCCCEEEEEEECCCCHHH
LISGPELISRGFVYMRESEEMISEANRIVTKQIEKSLQAGSDWTELKSNIRERLSVFLFE
HCCCHHHHHCCEEEEECHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
QTKRRPMILPIIMEI
HCCCCCEEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]