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The map label for this gene is cwlK [H]

Identifier: 229918678

GI number: 229918678

Start: 2927127

End: 2927780

Strand: Direct

Name: cwlK [H]

Synonym: EAT1b_2965

Alternate gene names: 229918678

Gene position: 2927127-2927780 (Clockwise)

Preceding gene: 229918677

Following gene: 229918679

Centisome position: 97.57

GC content: 50.92

Gene sequence:

>654_bases
ATGAGATGGACAGGCGTGTTATCTCTTGCGATGGTCGCCTTTCTGATTGGCATTACGCTCGCTTACTTTTATATGCAACC
GGAACCGGTGCCTTGGGTAGACGAGCCGACGTTCGAAGCGGATATGTGTGTACGGGACGTGGAGAAAGAGGATGCGATCT
TGGCAAGGACGAAGATTGACTTCGACCGGTTGCATCCGTTAGTCGCCAATCGGGGAGAATCGTTCATTCGATTGGCCCAT
TCTTGTCTAGACCTCGAAGTGCGCCTCACTTCAGGATACCGTTCTGCCAAGGAACAGAATGCGCTTTATGCGCAAGGACG
GTCTGAACCCGGGCAAGTGGTGACCAATGCGAAGGCGGGTCAGAGCTATCATAACTACGGTCTTGCTGTCGATTTCGTTA
TCATCCATAACAACAAGACCGACTATGACCTGAATACGGATCGTAATCGGAATGACATTCCTGACTGGCAAGAACTAGGG
GAGTTAGGAAAGGCGCTCGGCTTTGAATGGGGCGGAGACTGGAACTCATTCCCGGACTATCCTCACCTCCAAATGGACTT
CGGCTTGTCGATTCGTCAACTTGTTGCAGGGAAGCGACCTGATGAAAAAGAGACGGCACCGCTTGCTGATGCACTAAAAC
CTCTATTAGATTAG

Upstream 100 bases:

>100_bases
AAAAGGTCGGGGAATTCGACCTCGTCTTGAATCGTCCTTTGTATATGGCACTCGAAAAAGGGTTGCCACTCTTAAAAGGA
CTGCGTAAACGGTTAAGAAG

Downstream 100 bases:

>100_bases
ACGGAAATGTTGGCAATGAAGCCGTTTTCCGCTACAATATTCAGTAGCGATAAGGGGACGAAACAGAATATGAATCATTT
AGGAGGTTTTTGTTAGTGTC

Product: peptidase M15B and M15C DD-carboxypeptidase VanY/endolysin

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 217; Mature: 217

Protein sequence:

>217_residues
MRWTGVLSLAMVAFLIGITLAYFYMQPEPVPWVDEPTFEADMCVRDVEKEDAILARTKIDFDRLHPLVANRGESFIRLAH
SCLDLEVRLTSGYRSAKEQNALYAQGRSEPGQVVTNAKAGQSYHNYGLAVDFVIIHNNKTDYDLNTDRNRNDIPDWQELG
ELGKALGFEWGGDWNSFPDYPHLQMDFGLSIRQLVAGKRPDEKETAPLADALKPLLD

Sequences:

>Translated_217_residues
MRWTGVLSLAMVAFLIGITLAYFYMQPEPVPWVDEPTFEADMCVRDVEKEDAILARTKIDFDRLHPLVANRGESFIRLAH
SCLDLEVRLTSGYRSAKEQNALYAQGRSEPGQVVTNAKAGQSYHNYGLAVDFVIIHNNKTDYDLNTDRNRNDIPDWQELG
ELGKALGFEWGGDWNSFPDYPHLQMDFGLSIRQLVAGKRPDEKETAPLADALKPLLD
>Mature_217_residues
MRWTGVLSLAMVAFLIGITLAYFYMQPEPVPWVDEPTFEADMCVRDVEKEDAILARTKIDFDRLHPLVANRGESFIRLAH
SCLDLEVRLTSGYRSAKEQNALYAQGRSEPGQVVTNAKAGQSYHNYGLAVDFVIIHNNKTDYDLNTDRNRNDIPDWQELG
ELGKALGFEWGGDWNSFPDYPHLQMDFGLSIRQLVAGKRPDEKETAPLADALKPLLD

Specific function: Cleaves the linkage of the L-alanine-D-glutamic acid of B.subtilis cell wall [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M15C family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009045
- InterPro:   IPR012340
- InterPro:   IPR003709 [H]

Pfam domain/function: PF02557 VanY [H]

EC number: NA

Molecular weight: Translated: 24560; Mature: 24560

Theoretical pI: Translated: 4.65; Mature: 4.65

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRWTGVLSLAMVAFLIGITLAYFYMQPEPVPWVDEPTFEADMCVRDVEKEDAILARTKID
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHCCCCCHHHHHHCCC
FDRLHPLVANRGESFIRLAHSCLDLEVRLTSGYRSAKEQNALYAQGRSEPGQVVTNAKAG
HHHHHHHHHCCCHHHHHHHHHHHCEEEEECCCCHHHHHCCCCEECCCCCCCCEEECCCCC
QSYHNYGLAVDFVIIHNNKTDYDLNTDRNRNDIPDWQELGELGKALGFEWGGDWNSFPDY
CCHHCCCEEEEEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCC
PHLQMDFGLSIRQLVAGKRPDEKETAPLADALKPLLD
CCEEECCCCHHHHHHCCCCCCCHHCCCHHHHHHHHCC
>Mature Secondary Structure
MRWTGVLSLAMVAFLIGITLAYFYMQPEPVPWVDEPTFEADMCVRDVEKEDAILARTKID
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHCCCCCHHHHHHCCC
FDRLHPLVANRGESFIRLAHSCLDLEVRLTSGYRSAKEQNALYAQGRSEPGQVVTNAKAG
HHHHHHHHHCCCHHHHHHHHHHHCEEEEECCCCHHHHHCCCCEECCCCCCCCEEECCCCC
QSYHNYGLAVDFVIIHNNKTDYDLNTDRNRNDIPDWQELGELGKALGFEWGGDWNSFPDY
CCHHCCCEEEEEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCC
PHLQMDFGLSIRQLVAGKRPDEKETAPLADALKPLLD
CCEEECCCCHHHHHHCCCCCCCHHCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9274031; 9384377 [H]