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The map label for this gene is infB [H]

Identifier: 229918669

GI number: 229918669

Start: 2915846

End: 2918053

Strand: Direct

Name: infB [H]

Synonym: EAT1b_2956

Alternate gene names: 229918669

Gene position: 2915846-2918053 (Clockwise)

Preceding gene: 229918668

Following gene: 229918670

Centisome position: 97.2

GC content: 48.82

Gene sequence:

>2208_bases
TTGGGAAAACGCGTCTATGAATTTGCAAAAGAACAGAATGTAACGAGTAAACAAGTCATTACTCAATTAGAAAAATTGAA
CAAACCGGTGAAAAACCATATGGCCGTCCTCGATGAGGAAACGGTAAAACAATTGGACCGTGTGTTCAATCCTGAAAAGT
ATCGTGCTGAAAAACAAGCGGAAGCGAAAGCGACTTCAACATCGAATGAGCCGAAGCAAGAGAAAAAAGAAGCACAAGCG
TCACGTCCACAAGCGGGACAACAAAACCGTGGCGGTCAAAACAATCGCGGTGGACAAAATCGTAACGATAACCGAAACAA
CGACCCACGCAATAAAAAACGTGGAAAAGGGAAAGGCAAAGGCAAGCCGGTAAAAGCAGCTGTGCCACAAGAAGCCGATC
CAAACTCGAAGACGAGTCAGCGTAAAGCGGCACGTCTCGCGCAAGAGGCGGAGATGGGCAACGTCATCAAATATGACGAT
GTACTGAGCGTATCTGACCTTGCTTCTAAAATGAAAAAAAAGCCAAACGAAATCATCATGAAGTTGATGGGTCTCGGTGT
CATGGCAACCATCAACCAAACGCTTGATGATGAAACGGTTGAATTGCTCGCTGCAGAATTCGGCTTTGAGGTTGAAAAAG
AAGTTGTTGTCGATGAGACGGACTTTGAAACAGTTGAGATCGACTTCTCACAATATGACCTCGAAGAACGTCCAGCTGTC
GTTACCATCATGGGTCACGTCGACCACGGGAAAACGACACTTCTCGACTCGATTCGTAACACGAAAGTCGTCGCAGGAGA
AGCAGGTGGAATCACGCAGCACATCGGTGCTTATCAAGTCGAAGTCAACGATAAGAAAATCACATTCCTCGATACACCAG
GTCACGCGGCCTTCACGACGATGCGTGCCCGCGGTGCGGAAGTGACAGATATCGCGATCATCGTCGTTGCGGCTGACGAT
GGCGTCATGCCACAAACAGAGGAAGCGATCTCGCACGCCAAGGCGGCGAACGTTCCAATCATCGTAGCAGTCAACAAGAT
GGACAAAGAAGGGGCAAACCCTGACCGTGTCAAACAAGAGTTGACAGAGTTCGGTCTCATCCCTGAAGAGTGGGGCGGCG
AAACGATTTTCGTTCCGATTTCTGCCATTAAAGGCGAAGGGATCGATGAATTGCTCGAGATGATCCTTCTCGTATCTGAA
GTCGAAGAATACAAATCGACACCAGACATGCCAGCTCGTGGTTCAGTCATCGAGGCGAAACTCGACAAAGGACGTGGACC
GGTTGCGACACTTCTCGTTCAACACGGTACACTTCGTATCGGAGACTCAATCGTCGTCGGTAGCACGTTCGGTCGTGTCC
GTGCGATGGTCAACGATATCGGTCGCCGTGTGAAACAAGTCGGACCATCTACACCGATTGAGATCACTGGTTTGAACGAC
GTTCCACAAGCCGGTGACCAGTTCATCGTCTTCGAAGATGAGAAGAAAGCACGTGCAATCGGGGAGAAACGCTACCAACG
TTACCTCGAGTCACAGCGTCGTGAGTCGGCGAAAGTAAGTCTTGATGACTTGTTCAGCCGCATTCAAGAAGGTGAAGTAA
AAGACTTGAACGTCATCATCAAAGCAGACGTTCAAGGGTCAGCTGAAGCATTGGCTGGTTCACTCCGCAAGATTGATGTC
GAAGGCGTCAAAGTCAACATCGTGCACCAAGGTGTCGGTGCGATCACGGAAGGTGACGTCATCCTCGCGTCAGCTGCGAA
TGCCGTCATCATCGGATTCAACGTCCGTCCTGACGGAAACGCACGTTCGATGGCGGAACAAGAGAACGTTGAAATGCGTC
TCCACCGCATCATCTACAACGCGATCGAGGAAATCGAAAGCGCGATGAAAGGGATGCTTGACCCAGAGTTCGTGGAAGAA
ATCACAGGTCAAGTCGAAGTCCGTGACGTCTTCAAAGTATCGAAAGTCGGTGCGATTGCAGGTTGTTATGTGACAGAAGG
TAAAATCACACGTGACGCCGGTGTCCGTGTCATTCGTAACGGGATCGTGATTTACGAAGGTAAACTCGATACACTTCGCC
GTTTCAAAGATGACGTGAAAGAAGTGGCGACTGGTTATGAGTGTGGAATCAAAGTAGAGAAATTTGATGACATCAAAGTA
GACGATGTCATTGAAGCGTTCGTCATGAAAGAAGTCGAACGTAAATAA

Upstream 100 bases:

>100_bases
ACTCGGTAAAGACATGCGTGTCGTCGTATCGGTGACAGAATCCGGATTTGCGAACAAGCTTAAGCAACTTCTCTCTTAAC
TATTAACGGAGGTGGATGCT

Downstream 100 bases:

>100_bases
TGTGAGGAGGGATCACGTATGAAAATTCGTGCCCAACGTGTTGCCGAGCAAATGCGTAAAGAAATTACAGATGTCTTGTT
ACGGGAAGTCAGTGACCCGC

Product: translation initiation factor IF-2

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 735; Mature: 734

Protein sequence:

>735_residues
MGKRVYEFAKEQNVTSKQVITQLEKLNKPVKNHMAVLDEETVKQLDRVFNPEKYRAEKQAEAKATSTSNEPKQEKKEAQA
SRPQAGQQNRGGQNNRGGQNRNDNRNNDPRNKKRGKGKGKGKPVKAAVPQEADPNSKTSQRKAARLAQEAEMGNVIKYDD
VLSVSDLASKMKKKPNEIIMKLMGLGVMATINQTLDDETVELLAAEFGFEVEKEVVVDETDFETVEIDFSQYDLEERPAV
VTIMGHVDHGKTTLLDSIRNTKVVAGEAGGITQHIGAYQVEVNDKKITFLDTPGHAAFTTMRARGAEVTDIAIIVVAADD
GVMPQTEEAISHAKAANVPIIVAVNKMDKEGANPDRVKQELTEFGLIPEEWGGETIFVPISAIKGEGIDELLEMILLVSE
VEEYKSTPDMPARGSVIEAKLDKGRGPVATLLVQHGTLRIGDSIVVGSTFGRVRAMVNDIGRRVKQVGPSTPIEITGLND
VPQAGDQFIVFEDEKKARAIGEKRYQRYLESQRRESAKVSLDDLFSRIQEGEVKDLNVIIKADVQGSAEALAGSLRKIDV
EGVKVNIVHQGVGAITEGDVILASAANAVIIGFNVRPDGNARSMAEQENVEMRLHRIIYNAIEEIESAMKGMLDPEFVEE
ITGQVEVRDVFKVSKVGAIAGCYVTEGKITRDAGVRVIRNGIVIYEGKLDTLRRFKDDVKEVATGYECGIKVEKFDDIKV
DDVIEAFVMKEVERK

Sequences:

>Translated_735_residues
MGKRVYEFAKEQNVTSKQVITQLEKLNKPVKNHMAVLDEETVKQLDRVFNPEKYRAEKQAEAKATSTSNEPKQEKKEAQA
SRPQAGQQNRGGQNNRGGQNRNDNRNNDPRNKKRGKGKGKGKPVKAAVPQEADPNSKTSQRKAARLAQEAEMGNVIKYDD
VLSVSDLASKMKKKPNEIIMKLMGLGVMATINQTLDDETVELLAAEFGFEVEKEVVVDETDFETVEIDFSQYDLEERPAV
VTIMGHVDHGKTTLLDSIRNTKVVAGEAGGITQHIGAYQVEVNDKKITFLDTPGHAAFTTMRARGAEVTDIAIIVVAADD
GVMPQTEEAISHAKAANVPIIVAVNKMDKEGANPDRVKQELTEFGLIPEEWGGETIFVPISAIKGEGIDELLEMILLVSE
VEEYKSTPDMPARGSVIEAKLDKGRGPVATLLVQHGTLRIGDSIVVGSTFGRVRAMVNDIGRRVKQVGPSTPIEITGLND
VPQAGDQFIVFEDEKKARAIGEKRYQRYLESQRRESAKVSLDDLFSRIQEGEVKDLNVIIKADVQGSAEALAGSLRKIDV
EGVKVNIVHQGVGAITEGDVILASAANAVIIGFNVRPDGNARSMAEQENVEMRLHRIIYNAIEEIESAMKGMLDPEFVEE
ITGQVEVRDVFKVSKVGAIAGCYVTEGKITRDAGVRVIRNGIVIYEGKLDTLRRFKDDVKEVATGYECGIKVEKFDDIKV
DDVIEAFVMKEVERK
>Mature_734_residues
GKRVYEFAKEQNVTSKQVITQLEKLNKPVKNHMAVLDEETVKQLDRVFNPEKYRAEKQAEAKATSTSNEPKQEKKEAQAS
RPQAGQQNRGGQNNRGGQNRNDNRNNDPRNKKRGKGKGKGKPVKAAVPQEADPNSKTSQRKAARLAQEAEMGNVIKYDDV
LSVSDLASKMKKKPNEIIMKLMGLGVMATINQTLDDETVELLAAEFGFEVEKEVVVDETDFETVEIDFSQYDLEERPAVV
TIMGHVDHGKTTLLDSIRNTKVVAGEAGGITQHIGAYQVEVNDKKITFLDTPGHAAFTTMRARGAEVTDIAIIVVAADDG
VMPQTEEAISHAKAANVPIIVAVNKMDKEGANPDRVKQELTEFGLIPEEWGGETIFVPISAIKGEGIDELLEMILLVSEV
EEYKSTPDMPARGSVIEAKLDKGRGPVATLLVQHGTLRIGDSIVVGSTFGRVRAMVNDIGRRVKQVGPSTPIEITGLNDV
PQAGDQFIVFEDEKKARAIGEKRYQRYLESQRRESAKVSLDDLFSRIQEGEVKDLNVIIKADVQGSAEALAGSLRKIDVE
GVKVNIVHQGVGAITEGDVILASAANAVIIGFNVRPDGNARSMAEQENVEMRLHRIIYNAIEEIESAMKGMLDPEFVEEI
TGQVEVRDVFKVSKVGAIAGCYVTEGKITRDAGVRVIRNGIVIYEGKLDTLRRFKDDVKEVATGYECGIKVEKFDDIKVD
DVIEAFVMKEVERK

Specific function: One of the essential components for the initiation of protein synthesis. Protects formylmethionyl-tRNA from spontaneous hydrolysis and promotes its binding to the 30S ribosomal subunits. Also involved in the hydrolysis of GTP during the formation of the 7

COG id: COG0532

COG function: function code J; Translation initiation factor 2 (IF-2; GTPase)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IF-2 family [H]

Homologues:

Organism=Homo sapiens, GI53729339, Length=545, Percent_Identity=39.8165137614679, Blast_Score=382, Evalue=1e-105,
Organism=Homo sapiens, GI53729337, Length=545, Percent_Identity=39.8165137614679, Blast_Score=382, Evalue=1e-105,
Organism=Homo sapiens, GI84043963, Length=463, Percent_Identity=27.2138228941685, Blast_Score=131, Evalue=2e-30,
Organism=Homo sapiens, GI157426893, Length=243, Percent_Identity=27.9835390946502, Blast_Score=80, Evalue=1e-14,
Organism=Homo sapiens, GI18390331, Length=142, Percent_Identity=33.8028169014084, Blast_Score=70, Evalue=5e-12,
Organism=Homo sapiens, GI34147630, Length=261, Percent_Identity=30.2681992337165, Blast_Score=67, Evalue=4e-11,
Organism=Escherichia coli, GI1789559, Length=643, Percent_Identity=51.4774494556765, Blast_Score=621, Evalue=1e-179,
Organism=Escherichia coli, GI2367247, Length=229, Percent_Identity=32.7510917030568, Blast_Score=85, Evalue=1e-17,
Organism=Escherichia coli, GI1788922, Length=232, Percent_Identity=29.7413793103448, Blast_Score=78, Evalue=3e-15,
Organism=Escherichia coli, GI48994988, Length=197, Percent_Identity=30.4568527918782, Blast_Score=74, Evalue=3e-14,
Organism=Escherichia coli, GI1790412, Length=240, Percent_Identity=32.9166666666667, Blast_Score=71, Evalue=3e-13,
Organism=Escherichia coli, GI1789737, Length=240, Percent_Identity=32.9166666666667, Blast_Score=71, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI71994658, Length=622, Percent_Identity=34.7266881028939, Blast_Score=306, Evalue=4e-83,
Organism=Caenorhabditis elegans, GI212656558, Length=560, Percent_Identity=25.8928571428571, Blast_Score=130, Evalue=3e-30,
Organism=Caenorhabditis elegans, GI17557151, Length=219, Percent_Identity=31.9634703196347, Blast_Score=92, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17556456, Length=253, Percent_Identity=31.2252964426877, Blast_Score=78, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI25141371, Length=256, Percent_Identity=28.125, Blast_Score=71, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6324550, Length=608, Percent_Identity=36.6776315789474, Blast_Score=346, Evalue=8e-96,
Organism=Saccharomyces cerevisiae, GI6319282, Length=470, Percent_Identity=27.2340425531915, Blast_Score=132, Evalue=1e-31,
Organism=Saccharomyces cerevisiae, GI6324761, Length=278, Percent_Identity=29.8561151079137, Blast_Score=77, Evalue=8e-15,
Organism=Saccharomyces cerevisiae, GI6323320, Length=241, Percent_Identity=26.1410788381743, Blast_Score=71, Evalue=6e-13,
Organism=Drosophila melanogaster, GI28572034, Length=538, Percent_Identity=39.7769516728625, Blast_Score=358, Evalue=9e-99,
Organism=Drosophila melanogaster, GI24656849, Length=462, Percent_Identity=27.7056277056277, Blast_Score=126, Evalue=6e-29,
Organism=Drosophila melanogaster, GI160714833, Length=73, Percent_Identity=72.6027397260274, Blast_Score=116, Evalue=6e-26,
Organism=Drosophila melanogaster, GI78706572, Length=224, Percent_Identity=29.9107142857143, Blast_Score=83, Evalue=6e-16,
Organism=Drosophila melanogaster, GI281363316, Length=229, Percent_Identity=31.4410480349345, Blast_Score=75, Evalue=2e-13,
Organism=Drosophila melanogaster, GI17864358, Length=229, Percent_Identity=31.4410480349345, Blast_Score=75, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24582462, Length=154, Percent_Identity=31.8181818181818, Blast_Score=71, Evalue=3e-12,
Organism=Drosophila melanogaster, GI19922690, Length=224, Percent_Identity=28.5714285714286, Blast_Score=68, Evalue=2e-11,
Organism=Drosophila melanogaster, GI19921738, Length=136, Percent_Identity=38.9705882352941, Blast_Score=66, Evalue=9e-11,

Paralogues:

None

Copy number: 1150 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006847
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR000178
- InterPro:   IPR015760
- InterPro:   IPR023115
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF11987 IF-2; PF04760 IF2_N [H]

EC number: NA

Molecular weight: Translated: 80860; Mature: 80729

Theoretical pI: Translated: 5.16; Mature: 5.16

Prosite motif: PS01176 IF2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKRVYEFAKEQNVTSKQVITQLEKLNKPVKNHMAVLDEETVKQLDRVFNPEKYRAEKQA
CCCHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHCHHHHHHHHHHCCCHHHHHHHHH
EAKATSTSNEPKQEKKEAQASRPQAGQQNRGGQNNRGGQNRNDNRNNDPRNKKRGKGKGK
HHHHCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCCCCC
GKPVKAAVPQEADPNSKTSQRKAARLAQEAEMGNVIKYDDVLSVSDLASKMKKKPNEIIM
CCCEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEECCCCCCHHHHHHHHHCCHHHHHH
KLMGLGVMATINQTLDDETVELLAAEFGFEVEKEVVVDETDFETVEIDFSQYDLEERPAV
HHHCCCHHHHHHHHCCHHHHHHHHHHHCCCCCCEEEEECCCCEEEEEEHHHCCCCCCCEE
VTIMGHVDHGKTTLLDSIRNTKVVAGEAGGITQHIGAYQVEVNDKKITFLDTPGHAAFTT
EEEEEECCCCHHHHHHHHCCCEEEECCCCCCHHHCCEEEEEECCCEEEEEECCCCHHHHH
MRARGAEVTDIAIIVVAADDGVMPQTEEAISHAKAANVPIIVAVNKMDKEGANPDRVKQE
HHHCCCCEEEEEEEEEECCCCCCCCHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHH
LTEFGLIPEEWGGETIFVPISAIKGEGIDELLEMILLVSEVEEYKSTPDMPARGSVIEAK
HHHHCCCCHHCCCCEEEEEEHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEE
LDKGRGPVATLLVQHGTLRIGDSIVVGSTFGRVRAMVNDIGRRVKQVGPSTPIEITGLND
CCCCCCCHHHHHHHCCCEEECCEEEECCHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCC
VPQAGDQFIVFEDEKKARAIGEKRYQRYLESQRRESAKVSLDDLFSRIQEGEVKDLNVII
CCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCEEEEEE
KADVQGSAEALAGSLRKIDVEGVKVNIVHQGVGAITEGDVILASAANAVIIGFNVRPDGN
EECCCCCHHHHHHHHHEEEECCEEEEEEECCCCCCCCCCEEEEECCCEEEEEEEECCCCC
ARSMAEQENVEMRLHRIIYNAIEEIESAMKGMLDPEFVEEITGQVEVRDVFKVSKVGAIA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCEEHHHHHHHHHHCCEE
GCYVTEGKITRDAGVRVIRNGIVIYEGKLDTLRRFKDDVKEVATGYECGIKVEKFDDIKV
EEEEECCCEEHHHCHHEECCCEEEEECCHHHHHHHHHHHHHHHCCHHCCEEEECCCCCCH
DDVIEAFVMKEVERK
HHHHHHHHHHHHHCC
>Mature Secondary Structure 
GKRVYEFAKEQNVTSKQVITQLEKLNKPVKNHMAVLDEETVKQLDRVFNPEKYRAEKQA
CCHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHCHHHHHHHHHHCCCHHHHHHHHH
EAKATSTSNEPKQEKKEAQASRPQAGQQNRGGQNNRGGQNRNDNRNNDPRNKKRGKGKGK
HHHHCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCCCCC
GKPVKAAVPQEADPNSKTSQRKAARLAQEAEMGNVIKYDDVLSVSDLASKMKKKPNEIIM
CCCEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEECCCCCCHHHHHHHHHCCHHHHHH
KLMGLGVMATINQTLDDETVELLAAEFGFEVEKEVVVDETDFETVEIDFSQYDLEERPAV
HHHCCCHHHHHHHHCCHHHHHHHHHHHCCCCCCEEEEECCCCEEEEEEHHHCCCCCCCEE
VTIMGHVDHGKTTLLDSIRNTKVVAGEAGGITQHIGAYQVEVNDKKITFLDTPGHAAFTT
EEEEEECCCCHHHHHHHHCCCEEEECCCCCCHHHCCEEEEEECCCEEEEEECCCCHHHHH
MRARGAEVTDIAIIVVAADDGVMPQTEEAISHAKAANVPIIVAVNKMDKEGANPDRVKQE
HHHCCCCEEEEEEEEEECCCCCCCCHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHH
LTEFGLIPEEWGGETIFVPISAIKGEGIDELLEMILLVSEVEEYKSTPDMPARGSVIEAK
HHHHCCCCHHCCCCEEEEEEHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEE
LDKGRGPVATLLVQHGTLRIGDSIVVGSTFGRVRAMVNDIGRRVKQVGPSTPIEITGLND
CCCCCCCHHHHHHHCCCEEECCEEEECCHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCC
VPQAGDQFIVFEDEKKARAIGEKRYQRYLESQRRESAKVSLDDLFSRIQEGEVKDLNVII
CCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCEEEEEE
KADVQGSAEALAGSLRKIDVEGVKVNIVHQGVGAITEGDVILASAANAVIIGFNVRPDGN
EECCCCCHHHHHHHHHEEEECCEEEEEEECCCCCCCCCCEEEEECCCEEEEEEEECCCCC
ARSMAEQENVEMRLHRIIYNAIEEIESAMKGMLDPEFVEEITGQVEVRDVFKVSKVGAIA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCEEHHHHHHHHHHCCEE
GCYVTEGKITRDAGVRVIRNGIVIYEGKLDTLRRFKDDVKEVATGYECGIKVEKFDDIKV
EEEEECCCEEHHHCHHEECCCEEEEECCHHHHHHHHHHHHHHHCCHHCCEEEECCCCCCH
DDVIEAFVMKEVERK
HHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; In phosphorus-containing anhydrides [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11058132 [H]