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The map label for this gene is topA [H]

Identifier: 229918618

GI number: 229918618

Start: 2868329

End: 2870425

Strand: Direct

Name: topA [H]

Synonym: EAT1b_2905

Alternate gene names: 229918618

Gene position: 2868329-2870425 (Clockwise)

Preceding gene: 229918617

Following gene: 229918619

Centisome position: 95.61

GC content: 47.35

Gene sequence:

>2097_bases
ATGGCAAAATATCTGGTGATCGTCGAATCACCTGCGAAAGCAAAAACAATTAAACGTTATTTAGGTCCGAATTATATGGT
CAAGGCTTCGATGGGGCACGTGATTGACTTGCCTAAAAGTCAACTCGGGGTAGACGTCGAACACGACTTTGAACCGAAGT
ATATTACCATCCGTGGTAAAGGGCCGGTATTGAAAGAATTGAAGACAGCAGCGAAAAAAGCGACAAAAATCTTTCTCGCG
GCCGACCCCGATAGAGAAGGGGAAGCGATTGCTTGGCATTTAGCACGCGCACTCGGTGTCGATGAAACGACTGAATGTCG
TGTCGTCTTTAACGAAATCACGAAAGATGCGATTAAAGAATCGTTTAAACACCCACGTAAGATCAATCACGATCTTGTCG
ACGCACAACAAGCCCGACGCATTTTAGATCGTCTTGTCGGATACGGGATGAGCCCATTATTATGGAAGAAAGTCAAAAAA
GGATTGTCTGCAGGGCGAGTTCAATCGGTAGCCGTGAAGATGATTATCGACCGTGAACGTGAAATTAACGCGTTCGACCC
GGAAGAGTACTGGACGATTAAACTTGATTTAAATCATGATGGGGAAACGTTCGAGACTTCATTTTACGGAAAAGATGGGA
AGAAGATGGAATTGAAGTCTGAAGAAGATGCGCAATCTGTCCTCGATACCATCGATGATACGTTTAAAGTCATTGATGTG
ACGAGGAAAGAACGGAAGCGGAACGCTTCGCTACCGTTCACGACCAGTTCATTACAGCAGGATGCTGCCCGTAAATTGAA
TTTCCGTGCGAAGAAGACCATGATGCTCGCGCAGCAATTATATGAAGGAATCGATCTCGGCAAAAAAGAAGGTACAGTCG
GTCTGATTACGTATATGCGTACCGACTCGACACGTATCTCTGATTTGGCGAAGACGGAGGCGAAGGAATATATCGAATCG
ACATTCGGTGAAGAGTATGTCGCCTTACAGAAACAAAAAGAAAAGAAATCAGCGAATACCCAAGACGCGCACGAAGCGAT
TCGTCCGACTTCGGCACTCCGCGACCCAGCTTCCTTGAAGCCTTATCTGTCACGTGACCAGTTGCGGCTGTATAAATTGA
TTTGGGAACGATTGATTGCGAGCCAAATGGCACCAGCCGTGCTCGATACGGTCAAAATCGACGTCGAATCGAACGGGATG
ATTTTCCGTGCGAACGGGTCGACGGTCAAATTCCCTGGTTTCATGAAAGTGTACATCGAGTCGAAAGACGATGATATCGA
GGACACGAACCAAGAGAAGGAAGGATTATTGCCTCCTCTCGAAGTGGATGACACCGTGACATTTGAAACGATTGAACCGA
AACAACACTTCACACAACCGCCACCTCGTTATACAGAGGCGCGACTCGTTCGAGCGATGGAAGAACTCGGGATTGGGCGT
CCGTCGACGTACGCGCCGACACTCGACACGATCCAACGACGCGGTTATGTCGTTCTCGAAGAGAAGAAGTTTTTACCGAC
TGAACTTGGAGAACTCGTCATCGAGATGATTGATGAGTATTTCAACGACTTCATCACGGTCCAGTTCACAGCCGACATGG
AGACCCTTCTTGATTCGATTGAGCATAAGGAAGTCGAGTGGACGGAAGTCGTCGCGCCGATTTACCGATCGTTCGAGAAA
CGATTGAAGCGTGCCGAAGAAGAAATCGAGAAAATCGAAGTGAAGGATGAACCAGCCGGAATCGACTGTGAAGTCTGTGG
TTCACCGATGGTCATCAAAATGGGACGGTACGGCAAATTCATGGCGTGCTCGAACTTCCCGAACTGTACGAACACGAAAC
CGGTACAAGTCGAAATCGGTGTTCCGTGTCCGAACTGTAAGGATGGACAAATCGTGGAACGTCGCAGTAAAAAAGGTCGT
CTCTTCTATGGTTGCTCGAACTATCCGGAATGTGAATTCGTCTCATGGGATAAACCGGTTGCGAAACCGTGTCCTGTATG
CGACAGTTTGATGGTCGAGAAGAAAATTAAAGACGGCGTGAAATATCAATGTACGAACTGTACCCACACCGAGGTACATC
AGCAAGAGGAGGATTAA

Upstream 100 bases:

>100_bases
ATCAAATTATTCGAGATCTCAAGATTGTACGTTGACAAACCGGTGAGAACATGAATATTATGGTAGGGATTTAAATGACC
TCTAAAAGGGGAGCAGATGT

Downstream 100 bases:

>100_bases
TGTTGCAACAACGTGTAACTGTAATTGGGGCCGGATTGGCCGGCTCCGAAGCGGCTTGGCAACTCGCTAAGCGCGGTATT
CAAGTAGATTTATATGAAAT

Product: DNA topoisomerase I

Products: NA

Alternate protein names: DNA topoisomerase I; Omega-protein; Relaxing enzyme; Swivelase; Untwisting enzyme [H]

Number of amino acids: Translated: 698; Mature: 697

Protein sequence:

>698_residues
MAKYLVIVESPAKAKTIKRYLGPNYMVKASMGHVIDLPKSQLGVDVEHDFEPKYITIRGKGPVLKELKTAAKKATKIFLA
ADPDREGEAIAWHLARALGVDETTECRVVFNEITKDAIKESFKHPRKINHDLVDAQQARRILDRLVGYGMSPLLWKKVKK
GLSAGRVQSVAVKMIIDREREINAFDPEEYWTIKLDLNHDGETFETSFYGKDGKKMELKSEEDAQSVLDTIDDTFKVIDV
TRKERKRNASLPFTTSSLQQDAARKLNFRAKKTMMLAQQLYEGIDLGKKEGTVGLITYMRTDSTRISDLAKTEAKEYIES
TFGEEYVALQKQKEKKSANTQDAHEAIRPTSALRDPASLKPYLSRDQLRLYKLIWERLIASQMAPAVLDTVKIDVESNGM
IFRANGSTVKFPGFMKVYIESKDDDIEDTNQEKEGLLPPLEVDDTVTFETIEPKQHFTQPPPRYTEARLVRAMEELGIGR
PSTYAPTLDTIQRRGYVVLEEKKFLPTELGELVIEMIDEYFNDFITVQFTADMETLLDSIEHKEVEWTEVVAPIYRSFEK
RLKRAEEEIEKIEVKDEPAGIDCEVCGSPMVIKMGRYGKFMACSNFPNCTNTKPVQVEIGVPCPNCKDGQIVERRSKKGR
LFYGCSNYPECEFVSWDKPVAKPCPVCDSLMVEKKIKDGVKYQCTNCTHTEVHQQEED

Sequences:

>Translated_698_residues
MAKYLVIVESPAKAKTIKRYLGPNYMVKASMGHVIDLPKSQLGVDVEHDFEPKYITIRGKGPVLKELKTAAKKATKIFLA
ADPDREGEAIAWHLARALGVDETTECRVVFNEITKDAIKESFKHPRKINHDLVDAQQARRILDRLVGYGMSPLLWKKVKK
GLSAGRVQSVAVKMIIDREREINAFDPEEYWTIKLDLNHDGETFETSFYGKDGKKMELKSEEDAQSVLDTIDDTFKVIDV
TRKERKRNASLPFTTSSLQQDAARKLNFRAKKTMMLAQQLYEGIDLGKKEGTVGLITYMRTDSTRISDLAKTEAKEYIES
TFGEEYVALQKQKEKKSANTQDAHEAIRPTSALRDPASLKPYLSRDQLRLYKLIWERLIASQMAPAVLDTVKIDVESNGM
IFRANGSTVKFPGFMKVYIESKDDDIEDTNQEKEGLLPPLEVDDTVTFETIEPKQHFTQPPPRYTEARLVRAMEELGIGR
PSTYAPTLDTIQRRGYVVLEEKKFLPTELGELVIEMIDEYFNDFITVQFTADMETLLDSIEHKEVEWTEVVAPIYRSFEK
RLKRAEEEIEKIEVKDEPAGIDCEVCGSPMVIKMGRYGKFMACSNFPNCTNTKPVQVEIGVPCPNCKDGQIVERRSKKGR
LFYGCSNYPECEFVSWDKPVAKPCPVCDSLMVEKKIKDGVKYQCTNCTHTEVHQQEED
>Mature_697_residues
AKYLVIVESPAKAKTIKRYLGPNYMVKASMGHVIDLPKSQLGVDVEHDFEPKYITIRGKGPVLKELKTAAKKATKIFLAA
DPDREGEAIAWHLARALGVDETTECRVVFNEITKDAIKESFKHPRKINHDLVDAQQARRILDRLVGYGMSPLLWKKVKKG
LSAGRVQSVAVKMIIDREREINAFDPEEYWTIKLDLNHDGETFETSFYGKDGKKMELKSEEDAQSVLDTIDDTFKVIDVT
RKERKRNASLPFTTSSLQQDAARKLNFRAKKTMMLAQQLYEGIDLGKKEGTVGLITYMRTDSTRISDLAKTEAKEYIEST
FGEEYVALQKQKEKKSANTQDAHEAIRPTSALRDPASLKPYLSRDQLRLYKLIWERLIASQMAPAVLDTVKIDVESNGMI
FRANGSTVKFPGFMKVYIESKDDDIEDTNQEKEGLLPPLEVDDTVTFETIEPKQHFTQPPPRYTEARLVRAMEELGIGRP
STYAPTLDTIQRRGYVVLEEKKFLPTELGELVIEMIDEYFNDFITVQFTADMETLLDSIEHKEVEWTEVVAPIYRSFEKR
LKRAEEEIEKIEVKDEPAGIDCEVCGSPMVIKMGRYGKFMACSNFPNCTNTKPVQVEIGVPCPNCKDGQIVERRSKKGRL
FYGCSNYPECEFVSWDKPVAKPCPVCDSLMVEKKIKDGVKYQCTNCTHTEVHQQEED

Specific function: The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another [H]

COG id: COG0550

COG function: function code L; Topoisomerase IA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI10835218, Length=591, Percent_Identity=24.3654822335025, Blast_Score=125, Evalue=1e-28,
Organism=Homo sapiens, GI4507635, Length=666, Percent_Identity=22.8228228228228, Blast_Score=107, Evalue=4e-23,
Organism=Escherichia coli, GI1787529, Length=762, Percent_Identity=37.5328083989501, Blast_Score=484, Evalue=1e-138,
Organism=Escherichia coli, GI48994931, Length=107, Percent_Identity=41.1214953271028, Blast_Score=107, Evalue=2e-24,
Organism=Escherichia coli, GI1788061, Length=560, Percent_Identity=22.1428571428571, Blast_Score=89, Evalue=7e-19,
Organism=Caenorhabditis elegans, GI32563869, Length=585, Percent_Identity=23.5897435897436, Blast_Score=122, Evalue=7e-28,
Organism=Caenorhabditis elegans, GI17555378, Length=506, Percent_Identity=23.7154150197628, Blast_Score=96, Evalue=8e-20,
Organism=Saccharomyces cerevisiae, GI6323263, Length=505, Percent_Identity=24.950495049505, Blast_Score=116, Evalue=1e-26,
Organism=Drosophila melanogaster, GI24585251, Length=627, Percent_Identity=23.7639553429027, Blast_Score=131, Evalue=2e-30,
Organism=Drosophila melanogaster, GI24640096, Length=592, Percent_Identity=22.6351351351351, Blast_Score=91, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003601
- InterPro:   IPR013497
- InterPro:   IPR013824
- InterPro:   IPR013825
- InterPro:   IPR000380
- InterPro:   IPR003602
- InterPro:   IPR013498
- InterPro:   IPR005733
- InterPro:   IPR006171 [H]

Pfam domain/function: PF01131 Topoisom_bac; PF01751 Toprim; PF01396 zf-C4_Topoisom [H]

EC number: =5.99.1.2 [H]

Molecular weight: Translated: 79520; Mature: 79389

Theoretical pI: Translated: 6.07; Mature: 6.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKYLVIVESPAKAKTIKRYLGPNYMVKASMGHVIDLPKSQLGVDVEHDFEPKYITIRGK
CCEEEEEEECCHHHHHHHHHHCCCEEEEECCCCEEECCHHHHCCCCCCCCCCCEEEEECC
GPVLKELKTAAKKATKIFLAADPDREGEAIAWHLARALGVDETTECRVVFNEITKDAIKE
CCHHHHHHHHHHHHEEEEEEECCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHH
SFKHPRKINHDLVDAQQARRILDRLVGYGMSPLLWKKVKKGLSAGRVQSVAVKMIIDRER
HHCCCHHCCHHHHCHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCHHHHHHHHHHHCCCC
EINAFDPEEYWTIKLDLNHDGETFETSFYGKDGKKMELKSEEDAQSVLDTIDDTFKVIDV
CCCCCCCCCEEEEEEEECCCCCEEEEEEECCCCCEEECCCHHHHHHHHHHHHHHHHHHHH
TRKERKRNASLPFTTSSLQQDAARKLNFRAKKTMMLAQQLYEGIDLGKKEGTVGLITYMR
HHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEE
TDSTRISDLAKTEAKEYIESTFGEEYVALQKQKEKKSANTQDAHEAIRPTSALRDPASLK
CCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCHHHHHHHCCHHHHCCCCCCC
PYLSRDQLRLYKLIWERLIASQMAPAVLDTVKIDVESNGMIFRANGSTVKFPGFMKVYIE
CCCCHHHHHHHHHHHHHHHHHHHCHHHHHEEEEEECCCCEEEEECCCEEECCCEEEEEEE
SKDDDIEDTNQEKEGLLPPLEVDDTVTFETIEPKQHFTQPPPRYTEARLVRAMEELGIGR
CCCCCCCCCCCHHHCCCCCCCCCCCEEEEEECCHHHCCCCCCCHHHHHHHHHHHHHCCCC
PSTYAPTLDTIQRRGYVVLEEKKFLPTELGELVIEMIDEYFNDFITVQFTADMETLLDSI
CCCCCCCHHHHHHCCEEEEECCCCCHHHHHHHHHHHHHHHHCCEEEEEECCCHHHHHHHH
EHKEVEWTEVVAPIYRSFEKRLKRAEEEIEKIEVKDEPAGIDCEVCGSPMVIKMGRYGKF
HCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCEECCCCEEEEECCCCCE
MACSNFPNCTNTKPVQVEIGVPCPNCKDGQIVERRSKKGRLFYGCSNYPECEFVSWDKPV
EEECCCCCCCCCCCEEEEECCCCCCCCCCHHHHHHCCCCEEEECCCCCCCCCEECCCCCC
AKPCPVCDSLMVEKKIKDGVKYQCTNCTHTEVHQQEED
CCCCCCHHHHHHHHHHHCCCCEEECCCCHHHHHCCCCC
>Mature Secondary Structure 
AKYLVIVESPAKAKTIKRYLGPNYMVKASMGHVIDLPKSQLGVDVEHDFEPKYITIRGK
CEEEEEEECCHHHHHHHHHHCCCEEEEECCCCEEECCHHHHCCCCCCCCCCCEEEEECC
GPVLKELKTAAKKATKIFLAADPDREGEAIAWHLARALGVDETTECRVVFNEITKDAIKE
CCHHHHHHHHHHHHEEEEEEECCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHH
SFKHPRKINHDLVDAQQARRILDRLVGYGMSPLLWKKVKKGLSAGRVQSVAVKMIIDRER
HHCCCHHCCHHHHCHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCHHHHHHHHHHHCCCC
EINAFDPEEYWTIKLDLNHDGETFETSFYGKDGKKMELKSEEDAQSVLDTIDDTFKVIDV
CCCCCCCCCEEEEEEEECCCCCEEEEEEECCCCCEEECCCHHHHHHHHHHHHHHHHHHHH
TRKERKRNASLPFTTSSLQQDAARKLNFRAKKTMMLAQQLYEGIDLGKKEGTVGLITYMR
HHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEE
TDSTRISDLAKTEAKEYIESTFGEEYVALQKQKEKKSANTQDAHEAIRPTSALRDPASLK
CCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCHHHHHHHCCHHHHCCCCCCC
PYLSRDQLRLYKLIWERLIASQMAPAVLDTVKIDVESNGMIFRANGSTVKFPGFMKVYIE
CCCCHHHHHHHHHHHHHHHHHHHCHHHHHEEEEEECCCCEEEEECCCEEECCCEEEEEEE
SKDDDIEDTNQEKEGLLPPLEVDDTVTFETIEPKQHFTQPPPRYTEARLVRAMEELGIGR
CCCCCCCCCCCHHHCCCCCCCCCCCEEEEEECCHHHCCCCCCCHHHHHHHHHHHHHCCCC
PSTYAPTLDTIQRRGYVVLEEKKFLPTELGELVIEMIDEYFNDFITVQFTADMETLLDSI
CCCCCCCHHHHHHCCEEEEECCCCCHHHHHHHHHHHHHHHHCCEEEEEECCCHHHHHHHH
EHKEVEWTEVVAPIYRSFEKRLKRAEEEIEKIEVKDEPAGIDCEVCGSPMVIKMGRYGKF
HCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCEECCCCEEEEECCCCCE
MACSNFPNCTNTKPVQVEIGVPCPNCKDGQIVERRSKKGRLFYGCSNYPECEFVSWDKPV
EEECCCCCCCCCCCEEEEECCCCCCCCCCHHHHHHCCCCEEEECCCCCCCCCEECCCCCC
AKPCPVCDSLMVEKKIKDGVKYQCTNCTHTEVHQQEED
CCCCCCHHHHHHHHHHHCCCCEEECCCCHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11058132 [H]