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The map label for this gene is trmFO

Identifier: 229918619

GI number: 229918619

Start: 2870425

End: 2871735

Strand: Direct

Name: trmFO

Synonym: EAT1b_2906

Alternate gene names: 229918619

Gene position: 2870425-2871735 (Clockwise)

Preceding gene: 229918618

Following gene: 229918620

Centisome position: 95.68

GC content: 51.49

Gene sequence:

>1311_bases
ATGTTGCAACAACGTGTAACTGTAATTGGGGCCGGATTGGCCGGCTCCGAAGCGGCTTGGCAACTCGCTAAGCGCGGTAT
TCAAGTAGATTTATATGAAATGAGACCAGTTCGTCAAACGCCGGCGCACCACACGGATCAATTTGCGGAGCTCGTCTGTT
CGAACTCGCTTCGTGCGAACGGTCTTCAAAATGCCGTAGGTGTCTTGAAAGAAGAGATGCGTACGCTTGACTCGCTCATC
TTAAAAGCGGCGGACACGGCAAGTGTCCCGGCTGGCGGTGCCCTTGCCGTCGATCGTCATGAGTTTGCCGGATTCATCAC
CGAGAAGCTAAAGAACCACGAGAATGTCACCGTGCACAATGAAGAGCTCAAGGAGATTCCTGAAGGCATCGTCATCGTGG
CGACCGGTCCGTTGACCTCTCCTGACCTCTCTGCCTCGCTCAAGGCGTTCACGGGAGAAGATTATCTCTACTTCTATGAC
GCGGCAGCTCCGATTCTAGATGGCGATACGATTGATCGGGACAAAGTCTATTTGAAGTCGCGTTACGACAAAGGTGAAGC
CGCTTACTTGAACTGTCCGATGACAGAAGAAGAGTTTGACGTGTTTTACGAAGAGTTGATCAAGGCAGAAGTCGTTCCGC
TCAAAGAGTTTGAAAAAGAGATTTACTTTGAAGGATGTATGCCGTTTGAAGTCATGGCCGAGCGTGGGAAGAAGACGCTT
CTGTTCGGACCGATGAAGCCGGTCGGTTTAGAAGATCCGAAGACAGGAAAACGTCCATATGCCGTCGTTCAGCTTCGTCA
AGACAACTCGGCAGGCACACTTTATAACTTGGTCGGCTTCCAAACACACTTGAAGTGGGGCGAACAGAAGCGCATCATCC
GTCTCATCCCGGGGCTTGAGAACGCGGACATCGTTCGTTACGGTGTCATGCACCGTAACACGTTCATCAACTCACCGAGC
TTACTCAAGCCGACATATCAAGCGCGTACACGTGACACGCTCTTCTTCGCCGGTCAGATGACGGGGGTAGAAGGATATGT
GGAGTCGGCAGCGTCTGGACTTCTAGCCGGGATCAATGCGGCGAAGATGATCGCAGGTGAAGAACTTGTCGTTCTCCCAC
GTGAGACGATGCTTGGTTCGATGGCGCATTACATCACGACAGCTGACGGGAAGCACTTCCAACCGATGAACGCAAACTTC
GGATTGGTACCGTCACTAGAAGATGCTCCGAAAAAGATGAAGAAGCAAGAACGGTATGAGCGTTACGCGAACCGTGCGTT
AGAAACGATCCAACAGTATAAAGACCTTTGA

Upstream 100 bases:

>100_bases
TATGCGACAGTTTGATGGTCGAGAAGAAAATTAAAGACGGCGTGAAATATCAATGTACGAACTGTACCCACACCGAGGTA
CATCAGCAAGAGGAGGATTA

Downstream 100 bases:

>100_bases
ACAGGAGAGCCATTTTTTGGCTCTTTTGTTTTGAATTAAAATTTCAATTGTGTATTTTGTTTCATACTTTGAAAATTTTC
AGATTATTGAACGGCTATCT

Product: tRNA (uracil-5-)-methyltransferase Gid

Products: NA

Alternate protein names: Folate-dependent tRNA (uracil-5-)-methyltransferase; Folate-dependent tRNA(M-5-U54)-methyltransferase

Number of amino acids: Translated: 436; Mature: 436

Protein sequence:

>436_residues
MLQQRVTVIGAGLAGSEAAWQLAKRGIQVDLYEMRPVRQTPAHHTDQFAELVCSNSLRANGLQNAVGVLKEEMRTLDSLI
LKAADTASVPAGGALAVDRHEFAGFITEKLKNHENVTVHNEELKEIPEGIVIVATGPLTSPDLSASLKAFTGEDYLYFYD
AAAPILDGDTIDRDKVYLKSRYDKGEAAYLNCPMTEEEFDVFYEELIKAEVVPLKEFEKEIYFEGCMPFEVMAERGKKTL
LFGPMKPVGLEDPKTGKRPYAVVQLRQDNSAGTLYNLVGFQTHLKWGEQKRIIRLIPGLENADIVRYGVMHRNTFINSPS
LLKPTYQARTRDTLFFAGQMTGVEGYVESAASGLLAGINAAKMIAGEELVVLPRETMLGSMAHYITTADGKHFQPMNANF
GLVPSLEDAPKKMKKQERYERYANRALETIQQYKDL

Sequences:

>Translated_436_residues
MLQQRVTVIGAGLAGSEAAWQLAKRGIQVDLYEMRPVRQTPAHHTDQFAELVCSNSLRANGLQNAVGVLKEEMRTLDSLI
LKAADTASVPAGGALAVDRHEFAGFITEKLKNHENVTVHNEELKEIPEGIVIVATGPLTSPDLSASLKAFTGEDYLYFYD
AAAPILDGDTIDRDKVYLKSRYDKGEAAYLNCPMTEEEFDVFYEELIKAEVVPLKEFEKEIYFEGCMPFEVMAERGKKTL
LFGPMKPVGLEDPKTGKRPYAVVQLRQDNSAGTLYNLVGFQTHLKWGEQKRIIRLIPGLENADIVRYGVMHRNTFINSPS
LLKPTYQARTRDTLFFAGQMTGVEGYVESAASGLLAGINAAKMIAGEELVVLPRETMLGSMAHYITTADGKHFQPMNANF
GLVPSLEDAPKKMKKQERYERYANRALETIQQYKDL
>Mature_436_residues
MLQQRVTVIGAGLAGSEAAWQLAKRGIQVDLYEMRPVRQTPAHHTDQFAELVCSNSLRANGLQNAVGVLKEEMRTLDSLI
LKAADTASVPAGGALAVDRHEFAGFITEKLKNHENVTVHNEELKEIPEGIVIVATGPLTSPDLSASLKAFTGEDYLYFYD
AAAPILDGDTIDRDKVYLKSRYDKGEAAYLNCPMTEEEFDVFYEELIKAEVVPLKEFEKEIYFEGCMPFEVMAERGKKTL
LFGPMKPVGLEDPKTGKRPYAVVQLRQDNSAGTLYNLVGFQTHLKWGEQKRIIRLIPGLENADIVRYGVMHRNTFINSPS
LLKPTYQARTRDTLFFAGQMTGVEGYVESAASGLLAGINAAKMIAGEELVVLPRETMLGSMAHYITTADGKHFQPMNANF
GLVPSLEDAPKKMKKQERYERYANRALETIQQYKDL

Specific function: Catalyzes the folate-dependent formation of 5-methyl- uridine at position 54 (M-5-U54) in all tRNAs

COG id: COG1206

COG function: function code J; NAD(FAD)-utilizing enzyme possibly involved in translation

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MnmG family. TrmFO subfamily

Homologues:

Organism=Escherichia coli, GI2367273, Length=90, Percent_Identity=46.6666666666667, Blast_Score=76, Evalue=4e-15,
Organism=Saccharomyces cerevisiae, GI6321202, Length=173, Percent_Identity=28.9017341040462, Blast_Score=64, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRMFO_EXISA (C4L614)

Other databases:

- EMBL:   CP001615
- RefSeq:   YP_002887265.1
- GeneID:   7870161
- GenomeReviews:   CP001615_GR
- KEGG:   eat:EAT1b_2906
- OMA:   FEGCLPI
- ProtClustDB:   PRK05335
- GO:   GO:0005737
- HAMAP:   MF_01037
- InterPro:   IPR004417
- InterPro:   IPR002218
- InterPro:   IPR020595
- TIGRFAMs:   TIGR00137

Pfam domain/function: PF01134 GIDA

EC number: =2.1.1.74

Molecular weight: Translated: 48579; Mature: 48579

Theoretical pI: Translated: 5.71; Mature: 5.71

Prosite motif: PS01280 GIDA_1; PS01281 GIDA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLQQRVTVIGAGLAGSEAAWQLAKRGIQVDLYEMRPVRQTPAHHTDQFAELVCSNSLRAN
CCCCEEEEEECCCCCCHHHHHHHHCCCEEEEEECCCCCCCCCCCHHHHHHHHHCCCHHCC
GLQNAVGVLKEEMRTLDSLILKAADTASVPAGGALAVDRHEFAGFITEKLKNHENVTVHN
CHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECHHHHHHHHHHHHCCCCCEEEEH
EELKEIPEGIVIVATGPLTSPDLSASLKAFTGEDYLYFYDAAAPILDGDTIDRDKVYLKS
HHHHHCCCCEEEEEECCCCCCCCCCHHHEECCCCEEEEEECCCCCCCCCCCCCCHHHEEC
RYDKGEAAYLNCPMTEEEFDVFYEELIKAEVVPLKEFEKEIYFEGCMPFEVMAERGKKTL
CCCCCCEEEEECCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCHHHHHHCCCEEE
LFGPMKPVGLEDPKTGKRPYAVVQLRQDNSAGTLYNLVGFQTHLKWGEQKRIIRLIPGLE
EECCCCCCCCCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHCCCCCCCHHEECCCCC
NADIVRYGVMHRNTFINSPSLLKPTYQARTRDTLFFAGQMTGVEGYVESAASGLLAGINA
CCCEEEEEHHHCCCCCCCCCCCCCCHHHCCCCEEEEEEECCCHHHHHHHHHHHHHHCCCH
AKMIAGEELVVLPRETMLGSMAHYITTADGKHFQPMNANFGLVPSLEDAPKKMKKQERYE
HHHCCCCCEEEECHHHHHHHHHHHEECCCCCEECCCCCCCCCCCCCHHHHHHHHHHHHHH
RYANRALETIQQYKDL
HHHHHHHHHHHHHHCC
>Mature Secondary Structure
MLQQRVTVIGAGLAGSEAAWQLAKRGIQVDLYEMRPVRQTPAHHTDQFAELVCSNSLRAN
CCCCEEEEEECCCCCCHHHHHHHHCCCEEEEEECCCCCCCCCCCHHHHHHHHHCCCHHCC
GLQNAVGVLKEEMRTLDSLILKAADTASVPAGGALAVDRHEFAGFITEKLKNHENVTVHN
CHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECHHHHHHHHHHHHCCCCCEEEEH
EELKEIPEGIVIVATGPLTSPDLSASLKAFTGEDYLYFYDAAAPILDGDTIDRDKVYLKS
HHHHHCCCCEEEEEECCCCCCCCCCHHHEECCCCEEEEEECCCCCCCCCCCCCCHHHEEC
RYDKGEAAYLNCPMTEEEFDVFYEELIKAEVVPLKEFEKEIYFEGCMPFEVMAERGKKTL
CCCCCCEEEEECCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCHHHHHHCCCEEE
LFGPMKPVGLEDPKTGKRPYAVVQLRQDNSAGTLYNLVGFQTHLKWGEQKRIIRLIPGLE
EECCCCCCCCCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHCCCCCCCHHEECCCCC
NADIVRYGVMHRNTFINSPSLLKPTYQARTRDTLFFAGQMTGVEGYVESAASGLLAGINA
CCCEEEEEHHHCCCCCCCCCCCCCCHHHCCCCEEEEEEECCCHHHHHHHHHHHHHHCCCH
AKMIAGEELVVLPRETMLGSMAHYITTADGKHFQPMNANFGLVPSLEDAPKKMKKQERYE
HHHCCCCCEEEECHHHHHHHHHHHEECCCCCEECCCCCCCCCCCCCHHHHHHHHHHHHHH
RYANRALETIQQYKDL
HHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA