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The map label for this gene is trmD

Identifier: 229918608

GI number: 229918608

Start: 2860614

End: 2861330

Strand: Direct

Name: trmD

Synonym: EAT1b_2895

Alternate gene names: 229918608

Gene position: 2860614-2861330 (Clockwise)

Preceding gene: 229918607

Following gene: 229918609

Centisome position: 95.36

GC content: 53.97

Gene sequence:

>717_bases
ATGAAGATTGATGTATTGACGTTGTTCCCCGAGATGTTTCATGCGCTCGATCACTCCATCGTTGGTCGAGCGAAGACGCT
CGGGCAAGTGGAGATGTCGTTCCTCAACTTCCGGGACTTTTCAACGAACAAGCATCACAAGGTAGACGATTATCCGTACG
GCGGGGGCGCGGGCATGCTGCTCACCCCACAGCCGATCTTTGATGCGTTTCATTCCCTTGAGGCGAAGAGCCCACGGGTC
ATTCTGACGACGCCTACCGGAAAACCGTTCAATCAACGAATGGCCGAGGTATGGGCGAAGGAGGAACATCTGGTCTTTCT
ATGTGGACACTATGAAGGGTTCGATCAACGGATTCATGATGAACTGGCGACCGACGAGGTGTCGATCGGTGACTTCGTTT
TGACAGGTGGAGAGCTTGCGACGATGGTCATGATCGATGCGACCGTAAGGCTCATTCCGGATGTCCTTAATGCTCAGGCG
AGCCATGAAGACGACTCGTTCTCGACAGGTCTCTTGGAATACCCTCACTATACGCGACCGGCTGATTTTCGTGGATTGAC
CGTGCCGGACGTCCTCTTGTCGGGAAATCACGCCCGAATCGAGGCGTGGCGCCGGGAGCAATCGCTCGAACGCACGTATC
GACGACGTCCGGATTTGCTCGAACATGTCGAGTTGACGGATGTCGATAAAAAGTTCCTGCAATCGTTGCGCGATTGA

Upstream 100 bases:

>100_bases
AAGTCGGATGCGCTCATCCCTTATATAGAATCGGTCGTCCAAGAGATCGATGTCGATGCGAAACGTGTCGTCATCACGCC
AATCCCAGGAATGATTGACG

Downstream 100 bases:

>100_bases
GAAATATATGCTATGTTTGAGCATGTGGGTTAAACCCATCGATTACCGATGTTCCGCTATTGGATGCTTGGGCATATGAA
GATATGAACATTGAAGAAAA

Product: tRNA (guanine-N(1)-)-methyltransferase

Products: NA

Alternate protein names: M1G-methyltransferase; tRNA [GM37] methyltransferase

Number of amino acids: Translated: 238; Mature: 238

Protein sequence:

>238_residues
MKIDVLTLFPEMFHALDHSIVGRAKTLGQVEMSFLNFRDFSTNKHHKVDDYPYGGGAGMLLTPQPIFDAFHSLEAKSPRV
ILTTPTGKPFNQRMAEVWAKEEHLVFLCGHYEGFDQRIHDELATDEVSIGDFVLTGGELATMVMIDATVRLIPDVLNAQA
SHEDDSFSTGLLEYPHYTRPADFRGLTVPDVLLSGNHARIEAWRREQSLERTYRRRPDLLEHVELTDVDKKFLQSLRD

Sequences:

>Translated_238_residues
MKIDVLTLFPEMFHALDHSIVGRAKTLGQVEMSFLNFRDFSTNKHHKVDDYPYGGGAGMLLTPQPIFDAFHSLEAKSPRV
ILTTPTGKPFNQRMAEVWAKEEHLVFLCGHYEGFDQRIHDELATDEVSIGDFVLTGGELATMVMIDATVRLIPDVLNAQA
SHEDDSFSTGLLEYPHYTRPADFRGLTVPDVLLSGNHARIEAWRREQSLERTYRRRPDLLEHVELTDVDKKFLQSLRD
>Mature_238_residues
MKIDVLTLFPEMFHALDHSIVGRAKTLGQVEMSFLNFRDFSTNKHHKVDDYPYGGGAGMLLTPQPIFDAFHSLEAKSPRV
ILTTPTGKPFNQRMAEVWAKEEHLVFLCGHYEGFDQRIHDELATDEVSIGDFVLTGGELATMVMIDATVRLIPDVLNAQA
SHEDDSFSTGLLEYPHYTRPADFRGLTVPDVLLSGNHARIEAWRREQSLERTYRRRPDLLEHVELTDVDKKFLQSLRD

Specific function: Specifically methylates guanosine-37 in various tRNAs

COG id: COG0336

COG function: function code J; tRNA-(guanine-N1)-methyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase trmD family

Homologues:

Organism=Escherichia coli, GI1788959, Length=241, Percent_Identity=45.643153526971, Blast_Score=210, Evalue=5e-56,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRMD_EXISA (C4L603)

Other databases:

- EMBL:   CP001615
- RefSeq:   YP_002887254.1
- GeneID:   7870150
- GenomeReviews:   CP001615_GR
- KEGG:   eat:EAT1b_2895
- OMA:   VCGRFEG
- ProtClustDB:   PRK00026
- GO:   GO:0005737
- HAMAP:   MF_00605
- InterPro:   IPR016009
- InterPro:   IPR002649
- InterPro:   IPR023148
- Gene3D:   G3DSA:1.10.1270.20
- PIRSF:   PIRSF000386
- TIGRFAMs:   TIGR00088

Pfam domain/function: PF01746 tRNA_m1G_MT

EC number: =2.1.1.31

Molecular weight: Translated: 27124; Mature: 27124

Theoretical pI: Translated: 5.51; Mature: 5.51

Prosite motif: NA

Important sites: BINDING 109-109

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIDVLTLFPEMFHALDHSIVGRAKTLGQVEMSFLNFRDFSTNKHHKVDDYPYGGGAGML
CEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEE
LTPQPIFDAFHSLEAKSPRVILTTPTGKPFNQRMAEVWAKEEHLVFLCGHYEGFDQRIHD
ECCHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHCCCCCEEEEEECCCCHHHHHHH
ELATDEVSIGDFVLTGGELATMVMIDATVRLIPDVLNAQASHEDDSFSTGLLEYPHYTRP
HHHCCCCCCCCEEEECCCEEEHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCC
ADFRGLTVPDVLLSGNHARIEAWRREQSLERTYRRRPDLLEHVELTDVDKKFLQSLRD
CCCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MKIDVLTLFPEMFHALDHSIVGRAKTLGQVEMSFLNFRDFSTNKHHKVDDYPYGGGAGML
CEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEE
LTPQPIFDAFHSLEAKSPRVILTTPTGKPFNQRMAEVWAKEEHLVFLCGHYEGFDQRIHD
ECCHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHCCCCCEEEEEECCCCHHHHHHH
ELATDEVSIGDFVLTGGELATMVMIDATVRLIPDVLNAQASHEDDSFSTGLLEYPHYTRP
HHHCCCCCCCCEEEECCCEEEHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCC
ADFRGLTVPDVLLSGNHARIEAWRREQSLERTYRRRPDLLEHVELTDVDKKFLQSLRD
CCCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA