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The map label for this gene is rlmN [H]

Identifier: 229918582

GI number: 229918582

Start: 2835611

End: 2836696

Strand: Direct

Name: rlmN [H]

Synonym: EAT1b_2869

Alternate gene names: 229918582

Gene position: 2835611-2836696 (Clockwise)

Preceding gene: 229918581

Following gene: 229918583

Centisome position: 94.52

GC content: 49.08

Gene sequence:

>1086_bases
ATGAATCCAAAAGCAGTAGAAAAGAATCCACTCGTAGGCGCAAAACCGTCACTTTATTCGTTGACGTTCCCCGAACTTGA
GGCATGGGTGATCGAGGCAGGAGAAAAGGCATTCCGTGCGAAACAATTATATGATTGGATGTACGTCAAACGGGTCACGA
CTTTTGATGATATGACCAACGTTCCGAAAGCGCTTCGCGATAAATTAGAGGCATCGTTCCAATTGACGACGTTAAAAGAA
TTGGTCAAGCAGGAATCCCAGGACGGCACGATCAAGTTCTTGTTTGAACTTCAGGACGGGTATTCAATTGAAACGGTACT
CATGCGTCACGAATACGGAAACTCGATTTGTGTCACGACACAAGTCGGTTGCCGGATCGGATGTACGTTCTGTGCCTCGA
CACTCGGGGGCTTGAAACGAAACTTAGAAGCCGGAGAAATCACAGCCCAAGTGCTCGACGTGCAACGCGCATTAGACGCG
ACCGGAGAGCGCGTCGACTCCATCGTCGTCATGGGAATCGGCGAACCGTTCGACAACTACGATGAGTTAATGCGTTTCCT
ACGTACTGTCAACCATGACAATGGACTCAACATCGGAGCACGACACATCACAGTATCGACGAGTGGGATTGTTCCGAAGA
TTTACAAGTTTGCCGATGAAGGCATGCGAATCAACTTCGCCATTTCACTTCACGCACCGACGACTGAATTGCGTACTAAG
CTCATGCCAATCAACCGTGCCTTTGACTTGGACAAGTTGATGGATGCGGTCCGTTATTACACGGAGAAGAGCGGACGTCG
CATCACGTTTGAATATGGACTCTTTGGTGGTGTCAACGATACGGAAGAATATGCCCATCTCTTGGCAGACCTATTAAAAG
GGATCAAATGTCACGTCAACTTGATTCCCGTCAACCATGTACTCGAACGTGACTATGTACGGACACCGCGAGCTCAAATC
TTTGCCTTCGAAAAAGTGCTCAAAGACCGCAATGTGAACGTGACAATCCGTCGCGAGCAAGGGTCGGACATCGATGCCGC
TTGTGGACAGCTTCGAGCGAAGGAGCGCGAACAAGAAACGAGGTAA

Upstream 100 bases:

>100_bases
TCCGATGATGGAATCGGACCGTGCTGAACTAAAATTATTACCGACGACATTCGGGACCGACGGCTTCTATATCGCCGCAT
TTAAGAAAGAGGTATAAAAC

Downstream 100 bases:

>100_bases
TCAGAAAGATGGAATTAGCATTTCGAACAGATCGTGGACAAGTACGCACACAAAATGAGGATGCAGTTTTAATCTTAGGT
GATGCCATAACCGGGATTGC

Product: ribosomal RNA large subunit methyltransferase N

Products: NA

Alternate protein names: 23S rRNA m2A2503 methyltransferase [H]

Number of amino acids: Translated: 361; Mature: 361

Protein sequence:

>361_residues
MNPKAVEKNPLVGAKPSLYSLTFPELEAWVIEAGEKAFRAKQLYDWMYVKRVTTFDDMTNVPKALRDKLEASFQLTTLKE
LVKQESQDGTIKFLFELQDGYSIETVLMRHEYGNSICVTTQVGCRIGCTFCASTLGGLKRNLEAGEITAQVLDVQRALDA
TGERVDSIVVMGIGEPFDNYDELMRFLRTVNHDNGLNIGARHITVSTSGIVPKIYKFADEGMRINFAISLHAPTTELRTK
LMPINRAFDLDKLMDAVRYYTEKSGRRITFEYGLFGGVNDTEEYAHLLADLLKGIKCHVNLIPVNHVLERDYVRTPRAQI
FAFEKVLKDRNVNVTIRREQGSDIDAACGQLRAKEREQETR

Sequences:

>Translated_361_residues
MNPKAVEKNPLVGAKPSLYSLTFPELEAWVIEAGEKAFRAKQLYDWMYVKRVTTFDDMTNVPKALRDKLEASFQLTTLKE
LVKQESQDGTIKFLFELQDGYSIETVLMRHEYGNSICVTTQVGCRIGCTFCASTLGGLKRNLEAGEITAQVLDVQRALDA
TGERVDSIVVMGIGEPFDNYDELMRFLRTVNHDNGLNIGARHITVSTSGIVPKIYKFADEGMRINFAISLHAPTTELRTK
LMPINRAFDLDKLMDAVRYYTEKSGRRITFEYGLFGGVNDTEEYAHLLADLLKGIKCHVNLIPVNHVLERDYVRTPRAQI
FAFEKVLKDRNVNVTIRREQGSDIDAACGQLRAKEREQETR
>Mature_361_residues
MNPKAVEKNPLVGAKPSLYSLTFPELEAWVIEAGEKAFRAKQLYDWMYVKRVTTFDDMTNVPKALRDKLEASFQLTTLKE
LVKQESQDGTIKFLFELQDGYSIETVLMRHEYGNSICVTTQVGCRIGCTFCASTLGGLKRNLEAGEITAQVLDVQRALDA
TGERVDSIVVMGIGEPFDNYDELMRFLRTVNHDNGLNIGARHITVSTSGIVPKIYKFADEGMRINFAISLHAPTTELRTK
LMPINRAFDLDKLMDAVRYYTEKSGRRITFEYGLFGGVNDTEEYAHLLADLLKGIKCHVNLIPVNHVLERDYVRTPRAQI
FAFEKVLKDRNVNVTIRREQGSDIDAACGQLRAKEREQETR

Specific function: Specifically methylates position 2 of adenine 2503 in 23S rRNA [H]

COG id: COG0820

COG function: function code R; Predicted Fe-S-cluster redox enzyme

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. RlmN family [H]

Homologues:

Organism=Escherichia coli, GI1788865, Length=345, Percent_Identity=37.9710144927536, Blast_Score=239, Evalue=3e-64,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR007197
- InterPro:   IPR004383 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: =2.1.1.192 [H]

Molecular weight: Translated: 40914; Mature: 40914

Theoretical pI: Translated: 6.87; Mature: 6.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNPKAVEKNPLVGAKPSLYSLTFPELEAWVIEAGEKAFRAKQLYDWMYVKRVTTFDDMTN
CCCCCCCCCCCCCCCCCEEEEECCHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCHHHHHC
VPKALRDKLEASFQLTTLKELVKQESQDGTIKFLFELQDGYSIETVLMRHEYGNSICVTT
CHHHHHHHHHCCCHHHHHHHHHHCCCCCCEEEEEEEECCCCCEEEEEEECCCCCEEEEEE
QVGCRIGCTFCASTLGGLKRNLEAGEITAQVLDVQRALDATGERVDSIVVMGIGEPFDNY
ECCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCHHHCEEEEEECCCCCCCH
DELMRFLRTVNHDNGLNIGARHITVSTSGIVPKIYKFADEGMRINFAISLHAPTTELRTK
HHHHHHHHHHCCCCCCCCCEEEEEEECCCCHHHHHHHHCCCEEEEEEEEECCCHHHHHHH
LMPINRAFDLDKLMDAVRYYTEKSGRRITFEYGLFGGVNDTEEYAHLLADLLKGIKCHVN
HCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHHCCCEEEEE
LIPVNHVLERDYVRTPRAQIFAFEKVLKDRNVNVTIRREQGSDIDAACGQLRAKEREQET
EEEHHHHHHHHHHCCCHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHHCC
R
C
>Mature Secondary Structure
MNPKAVEKNPLVGAKPSLYSLTFPELEAWVIEAGEKAFRAKQLYDWMYVKRVTTFDDMTN
CCCCCCCCCCCCCCCCCEEEEECCHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCHHHHHC
VPKALRDKLEASFQLTTLKELVKQESQDGTIKFLFELQDGYSIETVLMRHEYGNSICVTT
CHHHHHHHHHCCCHHHHHHHHHHCCCCCCEEEEEEEECCCCCEEEEEEECCCCCEEEEEE
QVGCRIGCTFCASTLGGLKRNLEAGEITAQVLDVQRALDATGERVDSIVVMGIGEPFDNY
ECCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCHHHCEEEEEECCCCCCCH
DELMRFLRTVNHDNGLNIGARHITVSTSGIVPKIYKFADEGMRINFAISLHAPTTELRTK
HHHHHHHHHHCCCCCCCCCEEEEEEECCCCHHHHHHHHCCCEEEEEEEEECCCHHHHHHH
LMPINRAFDLDKLMDAVRYYTEKSGRRITFEYGLFGGVNDTEEYAHLLADLLKGIKCHVN
HCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHHCCCEEEEE
LIPVNHVLERDYVRTPRAQIFAFEKVLKDRNVNVTIRREQGSDIDAACGQLRAKEREQET
EEEHHHHHHHHHHCCCHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHHCC
R
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA