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The map label for this gene is pdhC [H]

Identifier: 229918500

GI number: 229918500

Start: 2754538

End: 2755827

Strand: Direct

Name: pdhC [H]

Synonym: EAT1b_2787

Alternate gene names: 229918500

Gene position: 2754538-2755827 (Clockwise)

Preceding gene: 229918499

Following gene: 229918501

Centisome position: 91.82

GC content: 47.52

Gene sequence:

>1290_bases
GTGGCAGTATTTGAATTTAAATTACCAGATATCGGTGAAGGTATTCACGAAGGTGAAATCGTCAAGTGGTTCGTAAAAGC
AGGGGATACGGTCAAAGAGGACGATGTTCTTTTAGAAGTACAAAACGACAAAGCAGTCGTTGAAATCCCAGCTCCAGTTG
ACGGTACAGTCAAAGAAGTTAAAGTTTCTGAAGGTACAGTTGCCGTAGTCGGCGACGTCCTCATTACGTTTGACATCGAA
GGCGACGCACCAGCAGGTGAAGAAGAAACACCTGAACAACCGAAAGCAGAAGAGAAGACAGAAGACGTAAAAGAAGATGT
GAAAGAAGACGCGCCACGCGACGTTCAACTTCACAAATCTGAACGTGTTATCGCTATGCCATCTGTCCGTAAATATGCAC
GTGAAAAAGGTGTCGACATCCGTGAAGTCAACGGTTCTGGCGACAACGGCCGTGTATTGAAAGAAGACATCGATGCATTC
GCGAACGGCGAAGCACCGTCTGCAGAAGCTACAACAGAGAAAACAGAATCTGTAGCACCAGCAGCAGCTGCGAAAACGGA
AATCAAGCCTTACGAGTCAGCGACTCCTGAACTTGAAACACGTGAGAAAATCCGTGGAATCCGTAAAGCGATCTCGAAAG
CGATGGTTAACTCGAAACACACTGCACCACACGTTACACTCATGGACGAAGTCGACGTTACAAAACTCGTTGCACTCCGC
AAAGACTTCAAACAAGTCGCGGCTGACCAAGGCGTGAAACTCACGTACTTGCCATTCGTCGTGAAAGCATTGACTGCTGC
AGCGAAAGCATTCCCAACAATCAACGCTTCAATCGATGACGTGAACGAAGAAATCGTCTACAAAAACTACTACAACATCG
GAATTGCAGCTGACACGGACAACGGACTCGTTGTACCAGTCGTAAAAGATGCAGACCGTAAATCAATCTATGCACTTGCT
ACAAACATTAACGAACTTGCAGGTAAAGCTCGTGAAGGTAAGCTCGCTGGGGAAGACATGAAAGGCGGATCGATCACAAT
CACAAACATCGGTTCAGCTGGTGGACAATGGTTCACACCTGTCATCAACCACCCAGAAGTTGCGATTCTCGGTATCGGCC
GCATCGCTGAGAAAGCTGTCGTGAAGAACGGTGAAATCGTTGCTGCGCCAGTACTTGCACTTTCATTCAGCTTTGACCAC
CGCCTCATCGACGGTGCGACTGCACAAAACGCTCTTAACATGGTGAAACGCTTGCTCGAAGACCCAGCACTTCTAATGAT
GGAGGGATAA

Upstream 100 bases:

>100_bases
TAAGGAAGTTGTGAACTTCTAAGATCGTGTCGAATAAACGATGAGGGAGAATTGTCTCCCTTGTCGTGTTTACATGATGA
ATTGATGGGAGGAAAAACCA

Downstream 100 bases:

>100_bases
GAACATGGTAGTAGGAGAATTCGCACAAGAAACTGATTTGCTTGTCATCGGTGCCGGTCCTGGTGGATACGTTGCCGCAA
TCCGTGGCGCTCAGCTCGGA

Product: branched-chain alpha-keto acid dehydrogenase subunit E2

Products: NA

Alternate protein names: Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex; E2; S complex, 48 kDa subunit [H]

Number of amino acids: Translated: 429; Mature: 428

Protein sequence:

>429_residues
MAVFEFKLPDIGEGIHEGEIVKWFVKAGDTVKEDDVLLEVQNDKAVVEIPAPVDGTVKEVKVSEGTVAVVGDVLITFDIE
GDAPAGEEETPEQPKAEEKTEDVKEDVKEDAPRDVQLHKSERVIAMPSVRKYAREKGVDIREVNGSGDNGRVLKEDIDAF
ANGEAPSAEATTEKTESVAPAAAAKTEIKPYESATPELETREKIRGIRKAISKAMVNSKHTAPHVTLMDEVDVTKLVALR
KDFKQVAADQGVKLTYLPFVVKALTAAAKAFPTINASIDDVNEEIVYKNYYNIGIAADTDNGLVVPVVKDADRKSIYALA
TNINELAGKAREGKLAGEDMKGGSITITNIGSAGGQWFTPVINHPEVAILGIGRIAEKAVVKNGEIVAAPVLALSFSFDH
RLIDGATAQNALNMVKRLLEDPALLMMEG

Sequences:

>Translated_429_residues
MAVFEFKLPDIGEGIHEGEIVKWFVKAGDTVKEDDVLLEVQNDKAVVEIPAPVDGTVKEVKVSEGTVAVVGDVLITFDIE
GDAPAGEEETPEQPKAEEKTEDVKEDVKEDAPRDVQLHKSERVIAMPSVRKYAREKGVDIREVNGSGDNGRVLKEDIDAF
ANGEAPSAEATTEKTESVAPAAAAKTEIKPYESATPELETREKIRGIRKAISKAMVNSKHTAPHVTLMDEVDVTKLVALR
KDFKQVAADQGVKLTYLPFVVKALTAAAKAFPTINASIDDVNEEIVYKNYYNIGIAADTDNGLVVPVVKDADRKSIYALA
TNINELAGKAREGKLAGEDMKGGSITITNIGSAGGQWFTPVINHPEVAILGIGRIAEKAVVKNGEIVAAPVLALSFSFDH
RLIDGATAQNALNMVKRLLEDPALLMMEG
>Mature_428_residues
AVFEFKLPDIGEGIHEGEIVKWFVKAGDTVKEDDVLLEVQNDKAVVEIPAPVDGTVKEVKVSEGTVAVVGDVLITFDIEG
DAPAGEEETPEQPKAEEKTEDVKEDVKEDAPRDVQLHKSERVIAMPSVRKYAREKGVDIREVNGSGDNGRVLKEDIDAFA
NGEAPSAEATTEKTESVAPAAAAKTEIKPYESATPELETREKIRGIRKAISKAMVNSKHTAPHVTLMDEVDVTKLVALRK
DFKQVAADQGVKLTYLPFVVKALTAAAKAFPTINASIDDVNEEIVYKNYYNIGIAADTDNGLVVPVVKDADRKSIYALAT
NINELAGKAREGKLAGEDMKGGSITITNIGSAGGQWFTPVINHPEVAILGIGRIAEKAVVKNGEIVAAPVLALSFSFDHR
LIDGATAQNALNMVKRLLEDPALLMMEG

Specific function: The B.subtilis PDH complex possesses also branched-chain 2-oxoacid dehydrogenase (BCDH) activity [H]

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI110671329, Length=431, Percent_Identity=37.8190255220418, Blast_Score=274, Evalue=1e-73,
Organism=Homo sapiens, GI31711992, Length=441, Percent_Identity=28.1179138321995, Blast_Score=162, Evalue=4e-40,
Organism=Homo sapiens, GI19923748, Length=231, Percent_Identity=35.4978354978355, Blast_Score=157, Evalue=1e-38,
Organism=Homo sapiens, GI203098753, Length=448, Percent_Identity=27.4553571428571, Blast_Score=141, Evalue=1e-33,
Organism=Homo sapiens, GI203098816, Length=448, Percent_Identity=27.6785714285714, Blast_Score=140, Evalue=2e-33,
Organism=Homo sapiens, GI260898739, Length=162, Percent_Identity=35.1851851851852, Blast_Score=89, Evalue=6e-18,
Organism=Escherichia coli, GI1786305, Length=440, Percent_Identity=35.9090909090909, Blast_Score=232, Evalue=3e-62,
Organism=Escherichia coli, GI1786946, Length=429, Percent_Identity=32.1678321678322, Blast_Score=219, Evalue=2e-58,
Organism=Caenorhabditis elegans, GI17537937, Length=433, Percent_Identity=36.0277136258661, Blast_Score=264, Evalue=8e-71,
Organism=Caenorhabditis elegans, GI25146366, Length=426, Percent_Identity=31.4553990610329, Blast_Score=184, Evalue=7e-47,
Organism=Caenorhabditis elegans, GI17560088, Length=450, Percent_Identity=29.3333333333333, Blast_Score=165, Evalue=4e-41,
Organism=Caenorhabditis elegans, GI17538894, Length=308, Percent_Identity=28.2467532467532, Blast_Score=117, Evalue=9e-27,
Organism=Saccharomyces cerevisiae, GI6320352, Length=426, Percent_Identity=32.6291079812207, Blast_Score=204, Evalue=2e-53,
Organism=Saccharomyces cerevisiae, GI6324258, Length=451, Percent_Identity=28.6031042128603, Blast_Score=159, Evalue=1e-39,
Organism=Drosophila melanogaster, GI18859875, Length=435, Percent_Identity=37.0114942528736, Blast_Score=256, Evalue=2e-68,
Organism=Drosophila melanogaster, GI24645909, Length=229, Percent_Identity=33.1877729257642, Blast_Score=132, Evalue=4e-31,
Organism=Drosophila melanogaster, GI24582497, Length=229, Percent_Identity=30.1310043668122, Blast_Score=123, Evalue=3e-28,
Organism=Drosophila melanogaster, GI20129315, Length=229, Percent_Identity=30.1310043668122, Blast_Score=122, Evalue=5e-28,

Paralogues:

None

Copy number: 1120 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 912 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.12 [H]

Molecular weight: Translated: 46160; Mature: 46029

Theoretical pI: Translated: 4.58; Mature: 4.58

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVFEFKLPDIGEGIHEGEIVKWFVKAGDTVKEDDVLLEVQNDKAVVEIPAPVDGTVKEV
CCEEEECCCCCCCCCCCCCEEEEEECCCCCCCCCCEEEEEECCCEEEEECCCCCCCCEEE
KVSEGTVAVVGDVLITFDIEGDAPAGEEETPEQPKAEEKTEDVKEDVKEDAPRDVQLHKS
EECCCCEEEEEEEEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEECC
ERVIAMPSVRKYAREKGVDIREVNGSGDNGRVLKEDIDAFANGEAPSAEATTEKTESVAP
CCEEECCHHHHHHHHCCCEEEEECCCCCCCCEEHHHHHHHCCCCCCCCCCCHHHHHHCCC
AAAAKTEIKPYESATPELETREKIRGIRKAISKAMVNSKHTAPHVTLMDEVDVTKLVALR
HHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHH
KDFKQVAADQGVKLTYLPFVVKALTAAAKAFPTINASIDDVNEEIVYKNYYNIGIAADTD
HHHHHHHHCCCCEEEHHHHHHHHHHHHHHHCCCCCCCHHCCCCCEEEEEEEEEEEEEECC
NGLVVPVVKDADRKSIYALATNINELAGKAREGKLAGEDMKGGSITITNIGSAGGQWFTP
CCEEEEEECCCCCCEEEEEECCHHHHHCCCCCCCCCCCCCCCCEEEEEECCCCCCCEECC
VINHPEVAILGIGRIAEKAVVKNGEIVAAPVLALSFSFDHRLIDGATAQNALNMVKRLLE
CCCCCCEEEEECCHHHHHHHHCCCCEEEEEHEEEEECCCCCEECCHHHHHHHHHHHHHHC
DPALLMMEG
CCCEEEECC
>Mature Secondary Structure 
AVFEFKLPDIGEGIHEGEIVKWFVKAGDTVKEDDVLLEVQNDKAVVEIPAPVDGTVKEV
CEEEECCCCCCCCCCCCCEEEEEECCCCCCCCCCEEEEEECCCEEEEECCCCCCCCEEE
KVSEGTVAVVGDVLITFDIEGDAPAGEEETPEQPKAEEKTEDVKEDVKEDAPRDVQLHKS
EECCCCEEEEEEEEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEECC
ERVIAMPSVRKYAREKGVDIREVNGSGDNGRVLKEDIDAFANGEAPSAEATTEKTESVAP
CCEEECCHHHHHHHHCCCEEEEECCCCCCCCEEHHHHHHHCCCCCCCCCCCHHHHHHCCC
AAAAKTEIKPYESATPELETREKIRGIRKAISKAMVNSKHTAPHVTLMDEVDVTKLVALR
HHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHH
KDFKQVAADQGVKLTYLPFVVKALTAAAKAFPTINASIDDVNEEIVYKNYYNIGIAADTD
HHHHHHHHCCCCEEEHHHHHHHHHHHHHHHCCCCCCCHHCCCCCEEEEEEEEEEEEEECC
NGLVVPVVKDADRKSIYALATNINELAGKAREGKLAGEDMKGGSITITNIGSAGGQWFTP
CCEEEEEECCCCCCEEEEEECCHHHHHCCCCCCCCCCCCCCCCEEEEEECCCCCCCEECC
VINHPEVAILGIGRIAEKAVVKNGEIVAAPVLALSFSFDHRLIDGATAQNALNMVKRLLE
CCCCCCEEEEECCHHHHHHHHCCCCEEEEEHEEEEECCCCCEECCHHHHHHHHHHHHHHC
DPALLMMEG
CCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1697575; 8969500; 9384377 [H]