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The map label for this gene is pdhD [H]

Identifier: 229918501

GI number: 229918501

Start: 2755832

End: 2757244

Strand: Direct

Name: pdhD [H]

Synonym: EAT1b_2788

Alternate gene names: 229918501

Gene position: 2755832-2757244 (Clockwise)

Preceding gene: 229918500

Following gene: 229918503

Centisome position: 91.86

GC content: 48.12

Gene sequence:

>1413_bases
ATGGTAGTAGGAGAATTCGCACAAGAAACTGATTTGCTTGTCATCGGTGCCGGTCCTGGTGGATACGTTGCCGCAATCCG
TGGCGCTCAGCTCGGAATGAAAGTAACAATCGTTGAGAAAGGCAACTTCGGTGGTGTCTGCTTGAACGTCGGATGTATCC
CATCGAAAGCGTTGATCACAGCAGGACACAACTTCCAACACGCTAAAGGACATGATTCAATGGGAATCACGTCTGACAAC
GTGTCAGTGGACTTCACAAAAGTTCAAGACTGGAAACAGTCAGTCGTTAACAAATTGACTGGCGGTGTCAAAGGTCTCCT
CAAAGGAAACAAAATTGAGATCGTTCAAGGTGAAGCGTTCTTCGCTTCTGAAGACACAGTTCGTGTCATCACTGAAGACA
GCTCAACTCCTTACAAATTCAAAAAAGCGATTGTCGCGACTGGTTCTACACCAATCGAGATCCCATCGTTCAAATGGTCT
AAACGTGTCCTTTCTTCAACTGGCGCGTTGGCACTTCCTGAAGTACCGAAGAAACTCGTCGTCATCGGCGGTGGATACAT
CGGTATGGAGCTCGGTACTGCATACGCTAACTTCGATACAGAAGTTGTCGTTGTAGAGGGCGCTAGCGACATCTTGTCTG
GTTTCGAACCACAAATGACGCAAATCGTTAAGAAGAAACTCAAGCAAAAAGGGAACGTCACAATCCACACGAATGCACTT
GCTAAAGGTGTTGAAGAAACGGAAGACGGCGTAACTGTTAAGTTTGAAGTAAACGGTGAAGAGCAATCTGTTGAAGCGGA
CTATGTCCTCGTCACAGTTGGTCGTCGTCCAAACACAGGCGACATCGGTCTTGAGAACGCGGACGTTAAAATCAGCGACC
GTGGAATCATCGAAATCGATGACCAGTGTAAAACATCGAACGAAAACATCTATGCAATCGGTGATATCGTTCCTGGACCA
CCACTTGCACACAAAGCATCTTACGAAGCGAAAATCGCAGCGGAAGCAGCAGCAGGTAAGCCAGCTTACCTCGACTACTC
TGCAATCCCTGCAGTCGTCTTCACAGATCCTGAACTCGCAACGGTTGGTTATACAGAGCCACTCGCAAAAGAAGAAGGAC
TCGAAATCACAGTTTCGAAATTCCCATTCGCAGCGAACGGTCGTGCACTTGCACTTGACGAGCCAGACGGCTTCATGAAG
CTCATCACACGCAAAGAGGACGGTCTCTTGATCGGTGCTCAAATCGCGGGTACGGGCGCTTCAGATATGATTGCTGAGCT
TGGCCTTGCAATCGAGGCTGGTATGACTGCTGAAGATATCGCACTTACAATCCACGCTCACCCATCACTCGGTGAGATGG
CTATGGAAGCTGCTGAAATCGCAATGGGTATGCCAATCCACGTTGTAAAATAA

Upstream 100 bases:

>100_bases
GACCACCGCCTCATCGACGGTGCGACTGCACAAAACGCTCTTAACATGGTGAAACGCTTGCTCGAAGACCCAGCACTTCT
AATGATGGAGGGATAAGAAC

Downstream 100 bases:

>100_bases
GAACGTCAATAAAACCTCTCTCACGATTCGTGGGAGAGGTTTTTCATGTAGGTGACGACACGCTCGACTTCCTTGAATCC
GACCTTCTCGTGAAAGCGTT

Product: dihydrolipoamide dehydrogenase

Products: NA

Alternate protein names: Dihydrolipoamide dehydrogenase; E3 component of pyruvate complex; S complex, 50 kDa subunit [H]

Number of amino acids: Translated: 470; Mature: 470

Protein sequence:

>470_residues
MVVGEFAQETDLLVIGAGPGGYVAAIRGAQLGMKVTIVEKGNFGGVCLNVGCIPSKALITAGHNFQHAKGHDSMGITSDN
VSVDFTKVQDWKQSVVNKLTGGVKGLLKGNKIEIVQGEAFFASEDTVRVITEDSSTPYKFKKAIVATGSTPIEIPSFKWS
KRVLSSTGALALPEVPKKLVVIGGGYIGMELGTAYANFDTEVVVVEGASDILSGFEPQMTQIVKKKLKQKGNVTIHTNAL
AKGVEETEDGVTVKFEVNGEEQSVEADYVLVTVGRRPNTGDIGLENADVKISDRGIIEIDDQCKTSNENIYAIGDIVPGP
PLAHKASYEAKIAAEAAAGKPAYLDYSAIPAVVFTDPELATVGYTEPLAKEEGLEITVSKFPFAANGRALALDEPDGFMK
LITRKEDGLLIGAQIAGTGASDMIAELGLAIEAGMTAEDIALTIHAHPSLGEMAMEAAEIAMGMPIHVVK

Sequences:

>Translated_470_residues
MVVGEFAQETDLLVIGAGPGGYVAAIRGAQLGMKVTIVEKGNFGGVCLNVGCIPSKALITAGHNFQHAKGHDSMGITSDN
VSVDFTKVQDWKQSVVNKLTGGVKGLLKGNKIEIVQGEAFFASEDTVRVITEDSSTPYKFKKAIVATGSTPIEIPSFKWS
KRVLSSTGALALPEVPKKLVVIGGGYIGMELGTAYANFDTEVVVVEGASDILSGFEPQMTQIVKKKLKQKGNVTIHTNAL
AKGVEETEDGVTVKFEVNGEEQSVEADYVLVTVGRRPNTGDIGLENADVKISDRGIIEIDDQCKTSNENIYAIGDIVPGP
PLAHKASYEAKIAAEAAAGKPAYLDYSAIPAVVFTDPELATVGYTEPLAKEEGLEITVSKFPFAANGRALALDEPDGFMK
LITRKEDGLLIGAQIAGTGASDMIAELGLAIEAGMTAEDIALTIHAHPSLGEMAMEAAEIAMGMPIHVVK
>Mature_470_residues
MVVGEFAQETDLLVIGAGPGGYVAAIRGAQLGMKVTIVEKGNFGGVCLNVGCIPSKALITAGHNFQHAKGHDSMGITSDN
VSVDFTKVQDWKQSVVNKLTGGVKGLLKGNKIEIVQGEAFFASEDTVRVITEDSSTPYKFKKAIVATGSTPIEIPSFKWS
KRVLSSTGALALPEVPKKLVVIGGGYIGMELGTAYANFDTEVVVVEGASDILSGFEPQMTQIVKKKLKQKGNVTIHTNAL
AKGVEETEDGVTVKFEVNGEEQSVEADYVLVTVGRRPNTGDIGLENADVKISDRGIIEIDDQCKTSNENIYAIGDIVPGP
PLAHKASYEAKIAAEAAAGKPAYLDYSAIPAVVFTDPELATVGYTEPLAKEEGLEITVSKFPFAANGRALALDEPDGFMK
LITRKEDGLLIGAQIAGTGASDMIAELGLAIEAGMTAEDIALTIHAHPSLGEMAMEAAEIAMGMPIHVVK

Specific function: Catalyzes the oxidation of dihydrolipoamide to lipoamide [H]

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=458, Percent_Identity=40.3930131004367, Blast_Score=321, Evalue=1e-87,
Organism=Homo sapiens, GI50301238, Length=466, Percent_Identity=28.1115879828326, Blast_Score=159, Evalue=7e-39,
Organism=Homo sapiens, GI33519430, Length=456, Percent_Identity=26.7543859649123, Blast_Score=127, Evalue=3e-29,
Organism=Homo sapiens, GI33519428, Length=456, Percent_Identity=26.7543859649123, Blast_Score=127, Evalue=3e-29,
Organism=Homo sapiens, GI33519426, Length=456, Percent_Identity=26.7543859649123, Blast_Score=127, Evalue=3e-29,
Organism=Homo sapiens, GI148277065, Length=456, Percent_Identity=26.7543859649123, Blast_Score=126, Evalue=4e-29,
Organism=Homo sapiens, GI148277071, Length=456, Percent_Identity=26.7543859649123, Blast_Score=126, Evalue=5e-29,
Organism=Homo sapiens, GI22035672, Length=459, Percent_Identity=27.6688453159041, Blast_Score=123, Evalue=4e-28,
Organism=Homo sapiens, GI291045266, Length=458, Percent_Identity=27.0742358078603, Blast_Score=121, Evalue=1e-27,
Organism=Homo sapiens, GI291045268, Length=450, Percent_Identity=25.7777777777778, Blast_Score=97, Evalue=3e-20,
Organism=Escherichia coli, GI1786307, Length=460, Percent_Identity=43.9130434782609, Blast_Score=362, Evalue=1e-101,
Organism=Escherichia coli, GI87081717, Length=447, Percent_Identity=29.7539149888143, Blast_Score=181, Evalue=1e-46,
Organism=Escherichia coli, GI1789915, Length=437, Percent_Identity=29.0617848970252, Blast_Score=169, Evalue=3e-43,
Organism=Escherichia coli, GI87082354, Length=460, Percent_Identity=26.9565217391304, Blast_Score=160, Evalue=2e-40,
Organism=Escherichia coli, GI1789065, Length=140, Percent_Identity=27.8571428571429, Blast_Score=62, Evalue=5e-11,
Organism=Caenorhabditis elegans, GI32565766, Length=461, Percent_Identity=40.5639913232104, Blast_Score=330, Evalue=1e-90,
Organism=Caenorhabditis elegans, GI71983419, Length=459, Percent_Identity=27.2331154684096, Blast_Score=148, Evalue=5e-36,
Organism=Caenorhabditis elegans, GI71983429, Length=459, Percent_Identity=27.2331154684096, Blast_Score=148, Evalue=6e-36,
Organism=Caenorhabditis elegans, GI17557007, Length=474, Percent_Identity=28.0590717299578, Blast_Score=144, Evalue=9e-35,
Organism=Caenorhabditis elegans, GI71982272, Length=483, Percent_Identity=24.6376811594203, Blast_Score=98, Evalue=8e-21,
Organism=Saccharomyces cerevisiae, GI6321091, Length=472, Percent_Identity=37.7118644067797, Blast_Score=290, Evalue=2e-79,
Organism=Saccharomyces cerevisiae, GI6325240, Length=478, Percent_Identity=26.1506276150628, Blast_Score=161, Evalue=3e-40,
Organism=Saccharomyces cerevisiae, GI6325166, Length=461, Percent_Identity=27.765726681128, Blast_Score=159, Evalue=7e-40,
Organism=Drosophila melanogaster, GI21358499, Length=469, Percent_Identity=41.36460554371, Blast_Score=322, Evalue=5e-88,
Organism=Drosophila melanogaster, GI24640549, Length=471, Percent_Identity=26.7515923566879, Blast_Score=119, Evalue=4e-27,
Organism=Drosophila melanogaster, GI24640553, Length=471, Percent_Identity=26.7515923566879, Blast_Score=119, Evalue=5e-27,
Organism=Drosophila melanogaster, GI24640551, Length=471, Percent_Identity=26.7515923566879, Blast_Score=118, Evalue=8e-27,
Organism=Drosophila melanogaster, GI17737741, Length=474, Percent_Identity=25.1054852320675, Blast_Score=112, Evalue=4e-25,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR006258
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.8.1.4 [H]

Molecular weight: Translated: 49490; Mature: 49490

Theoretical pI: Translated: 4.65; Mature: 4.65

Prosite motif: PS00076 PYRIDINE_REDOX_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVVGEFAQETDLLVIGAGPGGYVAAIRGAQLGMKVTIVEKGNFGGVCLNVGCIPSKALIT
CCCCCCCCCCCEEEEECCCCCEEEEEECCCCCEEEEEEECCCCCCEEEEECCCCCCEEEE
AGHNFQHAKGHDSMGITSDNVSVDFTKVQDWKQSVVNKLTGGVKGLLKGNKIEIVQGEAF
ECCCCCCCCCCCCCCEECCCCEEEEHHHHHHHHHHHHHHCCCHHHHCCCCEEEEEECCEE
FASEDTVRVITEDSSTPYKFKKAIVATGSTPIEIPSFKWSKRVLSSTGALALPEVPKKLV
EECCCEEEEEECCCCCCEEEEEEEEECCCCCEECCCCCHHHHHHHCCCCEECCCCCCEEE
VIGGGYIGMELGTAYANFDTEVVVVEGASDILSGFEPQMTQIVKKKLKQKGNVTIHTNAL
EEECCEEEEEECCEEECCCEEEEEEECCHHHHCCCCHHHHHHHHHHHHHCCCEEEEEHHH
AKGVEETEDGVTVKFEVNGEEQSVEADYVLVTVGRRPNTGDIGLENADVKISDRGIIEID
HCCCCCCCCCEEEEEEECCCCCCCCCCEEEEEECCCCCCCCCCCCCCCEEECCCCEEEEC
DQCKTSNENIYAIGDIVPGPPLAHKASYEAKIAAEAAAGKPAYLDYSAIPAVVFTDPELA
CCCCCCCCCEEEEECCCCCCCCCCCCCCCEEEEEHHCCCCCCEEEHHHCCEEEEECCCEE
TVGYTEPLAKEEGLEITVSKFPFAANGRALALDEPDGFMKLITRKEDGLLIGAQIAGTGA
EECCCCCCCCCCCCEEEEECCCCCCCCCEEEEECCCCCEEEEEECCCCEEEEEEEECCCH
SDMIAELGLAIEAGMTAEDIALTIHAHPSLGEMAMEAAEIAMGMPIHVVK
HHHHHHHCCEEECCCCCCCEEEEEEECCCHHHHHHHHHHHHCCCCEEEEC
>Mature Secondary Structure
MVVGEFAQETDLLVIGAGPGGYVAAIRGAQLGMKVTIVEKGNFGGVCLNVGCIPSKALIT
CCCCCCCCCCCEEEEECCCCCEEEEEECCCCCEEEEEEECCCCCCEEEEECCCCCCEEEE
AGHNFQHAKGHDSMGITSDNVSVDFTKVQDWKQSVVNKLTGGVKGLLKGNKIEIVQGEAF
ECCCCCCCCCCCCCCEECCCCEEEEHHHHHHHHHHHHHHCCCHHHHCCCCEEEEEECCEE
FASEDTVRVITEDSSTPYKFKKAIVATGSTPIEIPSFKWSKRVLSSTGALALPEVPKKLV
EECCCEEEEEECCCCCCEEEEEEEEECCCCCEECCCCCHHHHHHHCCCCEECCCCCCEEE
VIGGGYIGMELGTAYANFDTEVVVVEGASDILSGFEPQMTQIVKKKLKQKGNVTIHTNAL
EEECCEEEEEECCEEECCCEEEEEEECCHHHHCCCCHHHHHHHHHHHHHCCCEEEEEHHH
AKGVEETEDGVTVKFEVNGEEQSVEADYVLVTVGRRPNTGDIGLENADVKISDRGIIEID
HCCCCCCCCCEEEEEEECCCCCCCCCCEEEEEECCCCCCCCCCCCCCCEEECCCCEEEEC
DQCKTSNENIYAIGDIVPGPPLAHKASYEAKIAAEAAAGKPAYLDYSAIPAVVFTDPELA
CCCCCCCCCEEEEECCCCCCCCCCCCCCCEEEEEHHCCCCCCEEEHHHCCEEEEECCCEE
TVGYTEPLAKEEGLEITVSKFPFAANGRALALDEPDGFMKLITRKEDGLLIGAQIAGTGA
EECCCCCCCCCCCCEEEEECCCCCCCCCEEEEECCCCCEEEEEECCCCEEEEEEEECCCH
SDMIAELGLAIEAGMTAEDIALTIHAHPSLGEMAMEAAEIAMGMPIHVVK
HHHHHHHCCEEECCCCCCCEEEEEEECCCHHHHHHHHHHHHCCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1697575; 8969500; 9384377; 1936936 [H]