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The map label for this gene is rnjA [H]

Identifier: 229918494

GI number: 229918494

Start: 2746667

End: 2748334

Strand: Direct

Name: rnjA [H]

Synonym: EAT1b_2781

Alternate gene names: 229918494

Gene position: 2746667-2748334 (Clockwise)

Preceding gene: 229918492

Following gene: 229918495

Centisome position: 91.56

GC content: 48.44

Gene sequence:

>1668_bases
ATGAAGTTTGTTAAGAACCACCAAACAGCCGTGTTCGCATTGGGCGGACTCGGAGAAATCGGGAAGAACACATATGTTGT
TCAATTCCAAGATGAAATCATCGTCATCGATGCGGGAATCATGTTCCCAGAGGATGACTTGCTTGGAATCGACTATGTGA
TTCCTGATTACTCGTACTTGATTAAAAACAAGGACAAAGTAAAAGGGGTATTCATCACTCACGGTCACGAAGACCATATC
GGGGGAATTCCATTCTTATTGAAGCAAGTGCAATTGCCGATTTACGCGACGAAGCTCGCGCTCGGATTGATCAAAGTTAA
ACTTGAGGAGCATGGGTTGCTTCGTGAGGCGGAGCTCCATGAGATTGATGAGGATTCACTCATCAAATTCCGTAAGACGA
GCATCTCGTTCTTCCGCACGACGCACTCGATTCCGAACTCACTCGGTGTCGTCGTCAAGACGCCACAAGGGAACATCGTC
CATACCGGTGACTTCAAGTTCGACTTCACTCCGGTAGGCGAGCCGGCAGACCTTGCCAAGATGGCGGAAATCGGAAAAGA
AGGCGTACTTTGTCTCTTGTCCGATTCAACAAATGCAGAAGTGGAAGGCTTCACGATGAGTGAGAGCGTGGTCGGTGGGA
CGATTAGTGAGACGTTCCGGAAAGCGGACGGTCGAATCATCTTTGCGACGTTCGCATCGAACATCTATCGTTTGCAACAA
GTGGTCGATGCGTGTGCCGAGACAGGTCGTCATCTCATGATTTTCGGTCGTTCGATGGAGAAAGTCATGACGATCGGACA
AGAACTCGGTTTCATCAACGTGCCAAAGGGTATGATTATTGAGGCGAATCAGATGCGTCACTACGACCCGAATGAAATCG
CGATTCTTTGTACCGGATCGCAAGGGGAACCAATGGCAGCGCTCAGTCGTATTGCGAACGGGACGCACCGTCAAGTGTCA
ATCATTCCTGGGGATACGGTCATCTTCTCTTCTTCTCCAATCCCAGGTAACGCCATCTCGGTCGGGAAGACGATTGACCA
GTTGTATAAAGCGGGAGCGGATGTTGTCTACGGTAAGTTGACGAACATTCACACGTCAGGTCACGGTTCGCAACAAGAAC
AGTTGTTGATGATTCGTCTTCTGAATCCGAAATTCTTCATGCCGATTCACGGCGAGTACCGTATGTTGAAAAAGCATGCT
GAGCTTGCTGAATCGGTTGGAATCCCGAAAGAGAACAGTTTCGTTATGGATAATGGCGAAGTGCTTGCTGTCGACTCGGA
GTCGGCTGCGGTCACTGGTAAAATTTCTGCGAACGCTGTGTATATTGATGGGAATGGAATTGGTGATATCGGGAATATAG
TATTAAGGGATCGCCGAATTCTGTCTGAAGATGGACTTGTTGTCGTCGTCATCAGCATCGATGGCAAGACGCGCAAACTC
GTCTCAGGACCGGATATCATCTCACGTGGATTCGTCTACATGCGTGAATCAGGTAGTCTGATTCGTGATGGCGAACGATT
GTTGAAGAAAACCGTCGAACAAGGATTGTCCAATAAGCAGATGAAGTGGTCGGAGATGAAACAACTCGTTTCAGACACCC
TCACACCTTACTTGGCACAAAAGACGCGGAGACACCCAATGGTCCTGCCGATTATTATGGAAGTATGA

Upstream 100 bases:

>100_bases
CATGATATGAGCTATAATGAAAAAAGAATCTTACGGGCATATTCGAGAATCACTTATGTTTCACATAGATGTATTAATAA
TTGAAGTAAGGAGAGATTAA

Downstream 100 bases:

>100_bases
TCTGACGGGCTGTAGGAGACTACAGCTCGTTTTTTTCACCGCAAATGAGGAGGGGGAGCTTATGTCGAAGAAAAAGGGAA
AGAAAGGGTGGCTGAAATGG

Product: beta-lactamase domain protein

Products: NA

Alternate protein names: RNase J1 [H]

Number of amino acids: Translated: 555; Mature: 555

Protein sequence:

>555_residues
MKFVKNHQTAVFALGGLGEIGKNTYVVQFQDEIIVIDAGIMFPEDDLLGIDYVIPDYSYLIKNKDKVKGVFITHGHEDHI
GGIPFLLKQVQLPIYATKLALGLIKVKLEEHGLLREAELHEIDEDSLIKFRKTSISFFRTTHSIPNSLGVVVKTPQGNIV
HTGDFKFDFTPVGEPADLAKMAEIGKEGVLCLLSDSTNAEVEGFTMSESVVGGTISETFRKADGRIIFATFASNIYRLQQ
VVDACAETGRHLMIFGRSMEKVMTIGQELGFINVPKGMIIEANQMRHYDPNEIAILCTGSQGEPMAALSRIANGTHRQVS
IIPGDTVIFSSSPIPGNAISVGKTIDQLYKAGADVVYGKLTNIHTSGHGSQQEQLLMIRLLNPKFFMPIHGEYRMLKKHA
ELAESVGIPKENSFVMDNGEVLAVDSESAAVTGKISANAVYIDGNGIGDIGNIVLRDRRILSEDGLVVVVISIDGKTRKL
VSGPDIISRGFVYMRESGSLIRDGERLLKKTVEQGLSNKQMKWSEMKQLVSDTLTPYLAQKTRRHPMVLPIIMEV

Sequences:

>Translated_555_residues
MKFVKNHQTAVFALGGLGEIGKNTYVVQFQDEIIVIDAGIMFPEDDLLGIDYVIPDYSYLIKNKDKVKGVFITHGHEDHI
GGIPFLLKQVQLPIYATKLALGLIKVKLEEHGLLREAELHEIDEDSLIKFRKTSISFFRTTHSIPNSLGVVVKTPQGNIV
HTGDFKFDFTPVGEPADLAKMAEIGKEGVLCLLSDSTNAEVEGFTMSESVVGGTISETFRKADGRIIFATFASNIYRLQQ
VVDACAETGRHLMIFGRSMEKVMTIGQELGFINVPKGMIIEANQMRHYDPNEIAILCTGSQGEPMAALSRIANGTHRQVS
IIPGDTVIFSSSPIPGNAISVGKTIDQLYKAGADVVYGKLTNIHTSGHGSQQEQLLMIRLLNPKFFMPIHGEYRMLKKHA
ELAESVGIPKENSFVMDNGEVLAVDSESAAVTGKISANAVYIDGNGIGDIGNIVLRDRRILSEDGLVVVVISIDGKTRKL
VSGPDIISRGFVYMRESGSLIRDGERLLKKTVEQGLSNKQMKWSEMKQLVSDTLTPYLAQKTRRHPMVLPIIMEV
>Mature_555_residues
MKFVKNHQTAVFALGGLGEIGKNTYVVQFQDEIIVIDAGIMFPEDDLLGIDYVIPDYSYLIKNKDKVKGVFITHGHEDHI
GGIPFLLKQVQLPIYATKLALGLIKVKLEEHGLLREAELHEIDEDSLIKFRKTSISFFRTTHSIPNSLGVVVKTPQGNIV
HTGDFKFDFTPVGEPADLAKMAEIGKEGVLCLLSDSTNAEVEGFTMSESVVGGTISETFRKADGRIIFATFASNIYRLQQ
VVDACAETGRHLMIFGRSMEKVMTIGQELGFINVPKGMIIEANQMRHYDPNEIAILCTGSQGEPMAALSRIANGTHRQVS
IIPGDTVIFSSSPIPGNAISVGKTIDQLYKAGADVVYGKLTNIHTSGHGSQQEQLLMIRLLNPKFFMPIHGEYRMLKKHA
ELAESVGIPKENSFVMDNGEVLAVDSESAAVTGKISANAVYIDGNGIGDIGNIVLRDRRILSEDGLVVVVISIDGKTRKL
VSGPDIISRGFVYMRESGSLIRDGERLLKKTVEQGLSNKQMKWSEMKQLVSDTLTPYLAQKTRRHPMVLPIIMEV

Specific function: Cleaves the 5'-leader sequence of certain mRNAs. May play a role in maturation and stability of specific mRNAs. Is also required for the maturation of the 16S rRNA. Acts preferentially on 16S rRNA precursors after association of the 30S and 50S ribosomal

COG id: COG0595

COG function: function code R; Predicted hydrolase of the metallo-beta-lactamase superfamily

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the metallo-beta-lactamase superfamily. RNA-metabolizing metallo-beta-lactamase-like family. RNase J subfamily [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001279
- InterPro:   IPR011108
- InterPro:   IPR004613
- InterPro:   IPR001587 [H]

Pfam domain/function: PF00753 Lactamase_B; PF07521 RMMBL [H]

EC number: NA

Molecular weight: Translated: 61026; Mature: 61026

Theoretical pI: Translated: 6.62; Mature: 6.62

Prosite motif: PS01292 UPF0036

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFVKNHQTAVFALGGLGEIGKNTYVVQFQDEIIVIDAGIMFPEDDLLGIDYVIPDYSYL
CCCCCCCCEEEEEECCCCCCCCCEEEEEEECCEEEEECCEECCCCCCEEEEEECCCCCEE
IKNKDKVKGVFITHGHEDHIGGIPFLLKQVQLPIYATKLALGLIKVKLEEHGLLREAELH
ECCCCCEEEEEEECCCCCCCCCHHHHHHHHCCCHHHHHHHHEEEEEEEHHCCCEECCHHH
EIDEDSLIKFRKTSISFFRTTHSIPNSLGVVVKTPQGNIVHTGDFKFDFTPVGEPADLAK
CCCCCHHEEEEHHHHEEEHHHHCCCCCCEEEEECCCCCEEECCCCEEEECCCCCCHHHHH
MAEIGKEGVLCLLSDSTNAEVEGFTMSESVVGGTISETFRKADGRIIFATFASNIYRLQQ
HHHCCCCCEEEEEECCCCCEEEEEEECCHHCCCHHHHHHHHCCCCEEEEEHHHHHHHHHH
VVDACAETGRHLMIFGRSMEKVMTIGQELGFINVPKGMIIEANQMRHYDPNEIAILCTGS
HHHHHHHCCCEEEEECCCHHHHHHHHHHCCEEECCCCEEEEECCCCCCCCCCEEEEEECC
QGEPMAALSRIANGTHRQVSIIPGDTVIFSSSPIPGNAISVGKTIDQLYKAGADVVYGKL
CCCHHHHHHHHCCCCCCEEEEECCCEEEEECCCCCCCCEEHHHHHHHHHHCCCCEEEEEE
TNIHTSGHGSQQEQLLMIRLLNPKFFMPIHGEYRMLKKHAELAESVGIPKENSFVMDNGE
EEEEECCCCCCCCEEEEEEECCCEEEEEECCHHHHHHHHHHHHHHCCCCCCCCEEEECCC
VLAVDSESAAVTGKISANAVYIDGNGIGDIGNIVLRDRRILSEDGLVVVVISIDGKTRKL
EEEEECCCCEEEEEEECCEEEECCCCCCCHHHHEEECCEEECCCCEEEEEEEECCCCEEE
VSGPDIISRGFVYMRESGSLIRDGERLLKKTVEQGLSNKQMKWSEMKQLVSDTLTPYLAQ
ECCCCHHHCCEEEEECCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCHHHHH
KTRRHPMVLPIIMEV
HHCCCCEEEEEEEEC
>Mature Secondary Structure
MKFVKNHQTAVFALGGLGEIGKNTYVVQFQDEIIVIDAGIMFPEDDLLGIDYVIPDYSYL
CCCCCCCCEEEEEECCCCCCCCCEEEEEEECCEEEEECCEECCCCCCEEEEEECCCCCEE
IKNKDKVKGVFITHGHEDHIGGIPFLLKQVQLPIYATKLALGLIKVKLEEHGLLREAELH
ECCCCCEEEEEEECCCCCCCCCHHHHHHHHCCCHHHHHHHHEEEEEEEHHCCCEECCHHH
EIDEDSLIKFRKTSISFFRTTHSIPNSLGVVVKTPQGNIVHTGDFKFDFTPVGEPADLAK
CCCCCHHEEEEHHHHEEEHHHHCCCCCCEEEEECCCCCEEECCCCEEEECCCCCCHHHHH
MAEIGKEGVLCLLSDSTNAEVEGFTMSESVVGGTISETFRKADGRIIFATFASNIYRLQQ
HHHCCCCCEEEEEECCCCCEEEEEEECCHHCCCHHHHHHHHCCCCEEEEEHHHHHHHHHH
VVDACAETGRHLMIFGRSMEKVMTIGQELGFINVPKGMIIEANQMRHYDPNEIAILCTGS
HHHHHHHCCCEEEEECCCHHHHHHHHHHCCEEECCCCEEEEECCCCCCCCCCEEEEEECC
QGEPMAALSRIANGTHRQVSIIPGDTVIFSSSPIPGNAISVGKTIDQLYKAGADVVYGKL
CCCHHHHHHHHCCCCCCEEEEECCCEEEEECCCCCCCCEEHHHHHHHHHHCCCCEEEEEE
TNIHTSGHGSQQEQLLMIRLLNPKFFMPIHGEYRMLKKHAELAESVGIPKENSFVMDNGE
EEEEECCCCCCCCEEEEEEECCCEEEEEECCHHHHHHHHHHHHHHCCCCCCCCEEEECCC
VLAVDSESAAVTGKISANAVYIDGNGIGDIGNIVLRDRRILSEDGLVVVVISIDGKTRKL
EEEEECCCCEEEEEEECCEEEECCCCCCCHHHHEEECCEEECCCCEEEEEEEECCCCEEE
VSGPDIISRGFVYMRESGSLIRDGERLLKKTVEQGLSNKQMKWSEMKQLVSDTLTPYLAQ
ECCCCHHHCCEEEEECCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCHHHHH
KTRRHPMVLPIIMEV
HHCCCCEEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969500; 9384377 [H]