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The map label for this gene is ykuM [H]

Identifier: 229918488

GI number: 229918488

Start: 2740114

End: 2740992

Strand: Direct

Name: ykuM [H]

Synonym: EAT1b_2775

Alternate gene names: 229918488

Gene position: 2740114-2740992 (Clockwise)

Preceding gene: 229918486

Following gene: 229918489

Centisome position: 91.34

GC content: 48.81

Gene sequence:

>879_bases
ATGCAAGTTTCGGAAGTAGAAGTGTTAATTATTCTTGCGGAAGAATTGAATATGCGTAAAGCAGCCGAGCGATTGTTTGT
CTCACAACCAGCGCTCAGTCAACGGCTCGTAACCATCGAACATCAGTGGGGGAAGAAGATTTTCGTTCGCTCCACAAGAG
GGCTCGCATTGACGCCAGATGGAGAGAAAATCATCGAGTTCGCCAAAAAGATGTACAGTGAAGAACAAGAACTAAGGGGC
GAACTGACGGCCAACCAAGGAGAAGTGTACGGGACGTTGAAAATTGCCGTCGCCTCCATCATGGGACAGTATTGGCTACC
CAAGGTATTGAAGACGTTCGTCGAACGCTATCCGTCCGTCCGTGTCAAATTGGTCACCGGATGGTCGAGCGAAATGATGC
GTTACATGCTCGAAGAGAATTTTCATATCGGAATCATCCGAGGAAATCCGGATTGGCGAGGGGAACGGATGAAGTTGTTC
TCTGATTCCTTGCATTTGGTCGATCTGAAAATGACCGACCTCTCTCAACTAAAAAACAACGAGCGCTCGTTTATTCAATT
CAAGAGCGACTCGACGTATTACCAGGAAATATTAGAGTGGTGGCAGGAACGGTTTTCGAGCCCGCCTCTGACGACGCTGG
TCGTCGACCAAATCGAAACATGCAAACAGATGGCGTTACACGGAATCGGGTTCGCGATTCTTCCGGAATCGACCATCAAA
GATGTGAACGAGATGAATAAGACTCCATTGACGTCGTCGGGTGGCGTGCCGCTCGTCCGGGACACGTGGATGCTTTCAAC
AGAATCGATGTTGGAGCTGGCGCAAGTCCAGGCGTTTTGGAAAATCGTCGAAGAAATTACGAAGGAGACTGAGTTATGA

Upstream 100 bases:

>100_bases
CTCCTAACAATCAAATTGATGATTAAACATGATATGATACATTGAAACAAATACTTATTCAGTATACAATCTTTCAAACG
ATAGAGAAACAGGTGACGTG

Downstream 100 bases:

>100_bases
ATTGGGCAATTGAAGCGAGAAGACAGCTGCATTTAATTCCGGAAGCCGGATATGAAGAGTTCAAAACACAAGCGACACTT
GAGCAATTGATTCGATCGCT

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 292; Mature: 292

Protein sequence:

>292_residues
MQVSEVEVLIILAEELNMRKAAERLFVSQPALSQRLVTIEHQWGKKIFVRSTRGLALTPDGEKIIEFAKKMYSEEQELRG
ELTANQGEVYGTLKIAVASIMGQYWLPKVLKTFVERYPSVRVKLVTGWSSEMMRYMLEENFHIGIIRGNPDWRGERMKLF
SDSLHLVDLKMTDLSQLKNNERSFIQFKSDSTYYQEILEWWQERFSSPPLTTLVVDQIETCKQMALHGIGFAILPESTIK
DVNEMNKTPLTSSGGVPLVRDTWMLSTESMLELAQVQAFWKIVEEITKETEL

Sequences:

>Translated_292_residues
MQVSEVEVLIILAEELNMRKAAERLFVSQPALSQRLVTIEHQWGKKIFVRSTRGLALTPDGEKIIEFAKKMYSEEQELRG
ELTANQGEVYGTLKIAVASIMGQYWLPKVLKTFVERYPSVRVKLVTGWSSEMMRYMLEENFHIGIIRGNPDWRGERMKLF
SDSLHLVDLKMTDLSQLKNNERSFIQFKSDSTYYQEILEWWQERFSSPPLTTLVVDQIETCKQMALHGIGFAILPESTIK
DVNEMNKTPLTSSGGVPLVRDTWMLSTESMLELAQVQAFWKIVEEITKETEL
>Mature_292_residues
MQVSEVEVLIILAEELNMRKAAERLFVSQPALSQRLVTIEHQWGKKIFVRSTRGLALTPDGEKIIEFAKKMYSEEQELRG
ELTANQGEVYGTLKIAVASIMGQYWLPKVLKTFVERYPSVRVKLVTGWSSEMMRYMLEENFHIGIIRGNPDWRGERMKLF
SDSLHLVDLKMTDLSQLKNNERSFIQFKSDSTYYQEILEWWQERFSSPPLTTLVVDQIETCKQMALHGIGFAILPESTIK
DVNEMNKTPLTSSGGVPLVRDTWMLSTESMLELAQVQAFWKIVEEITKETEL

Specific function: This Protein Is A Positive Regulator Of Gene Expression For The Cysteine Regulon, A System Of 10 Or More Loci Involved In The Biosynthesis Of L-Cysteine From Inorganic Sulfate. The Inducer For Cysb Is N-Acetylserine. Cysb Inhibits Its Own Transcription.

COG id: COG0583

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787530, Length=163, Percent_Identity=29.4478527607362, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI1787879, Length=269, Percent_Identity=24.5353159851301, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1790208, Length=294, Percent_Identity=24.1496598639456, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI1787806, Length=250, Percent_Identity=21.2, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI1788296, Length=133, Percent_Identity=30.0751879699248, Blast_Score=68, Evalue=5e-13,
Organism=Escherichia coli, GI1787601, Length=288, Percent_Identity=21.1805555555556, Blast_Score=67, Evalue=9e-13,
Organism=Escherichia coli, GI1789173, Length=163, Percent_Identity=27.6073619631902, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI1790399, Length=134, Percent_Identity=27.6119402985075, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1788887, Length=246, Percent_Identity=24.390243902439, Blast_Score=65, Evalue=4e-12,
Organism=Escherichia coli, GI145693105, Length=148, Percent_Identity=25.6756756756757, Blast_Score=65, Evalue=4e-12,
Organism=Escherichia coli, GI1787128, Length=145, Percent_Identity=26.2068965517241, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI157672245, Length=166, Percent_Identity=27.710843373494, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1788706, Length=127, Percent_Identity=30.7086614173228, Blast_Score=62, Evalue=4e-11,
Organism=Escherichia coli, GI1787589, Length=148, Percent_Identity=24.3243243243243, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33718; Mature: 33718

Theoretical pI: Translated: 5.29; Mature: 5.29

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQVSEVEVLIILAEELNMRKAAERLFVSQPALSQRLVTIEHQWGKKIFVRSTRGLALTPD
CCCCCEEEEEEEHHHHHHHHHHHHHHHCCCHHHHHEEEEECCCCCEEEEEECCCEEECCC
GEKIIEFAKKMYSEEQELRGELTANQGEVYGTLKIAVASIMGQYWLPKVLKTFVERYPSV
HHHHHHHHHHHHCCHHHHHHHCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCE
RVKLVTGWSSEMMRYMLEENFHIGIIRGNPDWRGERMKLFSDSLHLVDLKMTDLSQLKNN
EEEEEECCHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHCCCEEEEEEEHHHHHHHHCC
ERSFIQFKSDSTYYQEILEWWQERFSSPPLTTLVVDQIETCKQMALHGIGFAILPESTIK
CCEEEEECCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCEEECCCHHHH
DVNEMNKTPLTSSGGVPLVRDTWMLSTESMLELAQVQAFWKIVEEITKETEL
HHHHCCCCCCCCCCCCCEEEHHHHHCHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MQVSEVEVLIILAEELNMRKAAERLFVSQPALSQRLVTIEHQWGKKIFVRSTRGLALTPD
CCCCCEEEEEEEHHHHHHHHHHHHHHHCCCHHHHHEEEEECCCCCEEEEEECCCEEECCC
GEKIIEFAKKMYSEEQELRGELTANQGEVYGTLKIAVASIMGQYWLPKVLKTFVERYPSV
HHHHHHHHHHHHCCHHHHHHHCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCE
RVKLVTGWSSEMMRYMLEENFHIGIIRGNPDWRGERMKLFSDSLHLVDLKMTDLSQLKNN
EEEEEECCHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHCCCEEEEEEEHHHHHHHHCC
ERSFIQFKSDSTYYQEILEWWQERFSSPPLTTLVVDQIETCKQMALHGIGFAILPESTIK
CCEEEEECCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCEEECCCHHHH
DVNEMNKTPLTSSGGVPLVRDTWMLSTESMLELAQVQAFWKIVEEITKETEL
HHHHCCCCCCCCCCCCCEEEHHHHHCHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]