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The map label for this gene is cls [H]

Identifier: 229918461

GI number: 229918461

Start: 2719055

End: 2720566

Strand: Direct

Name: cls [H]

Synonym: EAT1b_2748

Alternate gene names: 229918461

Gene position: 2719055-2720566 (Clockwise)

Preceding gene: 229918459

Following gene: 229918462

Centisome position: 90.64

GC content: 45.3

Gene sequence:

>1512_bases
ATGTTGCGACGCATACAAGTACTCTTTTCCCTCATTTTGCTCGCAGGACTCATTGCGATATTATCGTATTACTGGAGCTC
ACAAGTAATCGGATTGTTCTCGATTCTCGTCTTTTTGACAACGTTCAGCATATTGCTCGTCATCTTACTTGAAAATCGAA
ATCCACAACGTACGCTCATTTGGGCAATTGTCATGATTGGTTTTCCAGTCATCGGATTATTCGCATACTTTGTATTCGGT
CAGAACTATCGCCGTAAACGTATGTTCAAAGAAAAAGCGATGCTTGATGAAGAGACGTACTTGGCCTATCGTCAGAAAGC
GATGACGTTGTCGCCGTCCCTCATGTTCGCCCACGACGATTACGAGAAGCTGATGCATTTGACTTCTTCGTTGAACCAGT
TACCGGTCTCATCGAATACGTATACGCAAATCTTGACGAACGGCCGTGAAAAGTTTTCGAAGTTATTGCCGGCTCTGAAA
GGAGCGTCCCGACACATCCATCTAGAGTATTATATTTTCCGGGAAGATCGCATTTCTCGGGAAATACAGCGGATCTTGAT
CGAAAAAGCGAAGGCGGGGGTCGAAGTCCGATTTTTATATGATGCCGTCGGGTCCATTCATACGAGCGGCACGTTCTTTG
AAGAGATGAAGCAAGCCGGTGTCCAAGTCCAGGCATTCTTTCCAGTAGTATTGCCGCTCGTTTCAAGTAAGACGAACTAT
CGAAACCACCGAAAAATCGTGGTCATCGATGGTACGGAGGCATTCACAGGTGGCTTGAATGTAGGCGATGAATACTTGGG
TGAGCACTCTAAGTTCGGGTTTTGGCGCGACACCCATCTGTATGTCAGAGGGGAAGGGGTATCTGAACTGCAATTGATTT
TCTTGCAAGATTGGTATTACATGACCGGGGAACGCCTGTTCACACCGTTCTATCTAGAGCCGCTTGAAACGGTAAGAAGT
GCGAGCGGAGGGGTCCAAATCGTTGCAAGTGGACCGGATGAACCGTATGAGACGATGAAGTCCATTTACTTTTCACTCAT
CAATGCTGCGAAAAAATCTGTCTACATTTCGTCACCTTACTTGATTCCAGATGAGGACATTATGAGTGCCTTGAAGACGG
CGTCATTATCAGGAATCGATGTCCGCATCGTCTTACCGAGTTATCCGGACCATAAAATCGTGTTCTACGCGAGTCGGTCC
TATTACGAAGAACTGTTACTCGCCGGGGTGAAGATTTATCTATATGAAAAGGGATTTATGCATTCAAAAGTCATCGTGGT
GGATGATGGATTAGCCACGATCGGGACGGCGAACATGGATTTTCGGAGCTTTCATTTGAACTTCGAGGTCAATGCGCTCC
TATATAACACGAATTCGGTCCATCAGCTGAAACAAGACTTATATGATGATTTCGAAGGGTCTCACGAACTCGTATTAGAA
GAGTTCGCGAAACGGTCATTCTTTATCAAACTCGTTGAATCATTAGCTAGAATGTTTTCACCATTGCTATGA

Upstream 100 bases:

>100_bases
ATTAGAAAAGTCCCCATAATTTATTCGCTCCCCTCAAAAAGATGATGTTACGATGTATAATTAATGATAATACATGACAG
AAACGAGGTCAGCTTATAAT

Downstream 100 bases:

>100_bases
GGAGGGAGCAAGATGCGGTTTGCAATCGATCGGGAAGGGGATATCATTGATTCCTTTACATTAAATCGGATGCAAGCAGA
GCAACTTGCTCCTTTTCGAT

Product: cardiolipin synthetase

Products: NA

Alternate protein names: CL synthase 2 [H]

Number of amino acids: Translated: 503; Mature: 503

Protein sequence:

>503_residues
MLRRIQVLFSLILLAGLIAILSYYWSSQVIGLFSILVFLTTFSILLVILLENRNPQRTLIWAIVMIGFPVIGLFAYFVFG
QNYRRKRMFKEKAMLDEETYLAYRQKAMTLSPSLMFAHDDYEKLMHLTSSLNQLPVSSNTYTQILTNGREKFSKLLPALK
GASRHIHLEYYIFREDRISREIQRILIEKAKAGVEVRFLYDAVGSIHTSGTFFEEMKQAGVQVQAFFPVVLPLVSSKTNY
RNHRKIVVIDGTEAFTGGLNVGDEYLGEHSKFGFWRDTHLYVRGEGVSELQLIFLQDWYYMTGERLFTPFYLEPLETVRS
ASGGVQIVASGPDEPYETMKSIYFSLINAAKKSVYISSPYLIPDEDIMSALKTASLSGIDVRIVLPSYPDHKIVFYASRS
YYEELLLAGVKIYLYEKGFMHSKVIVVDDGLATIGTANMDFRSFHLNFEVNALLYNTNSVHQLKQDLYDDFEGSHELVLE
EFAKRSFFIKLVESLARMFSPLL

Sequences:

>Translated_503_residues
MLRRIQVLFSLILLAGLIAILSYYWSSQVIGLFSILVFLTTFSILLVILLENRNPQRTLIWAIVMIGFPVIGLFAYFVFG
QNYRRKRMFKEKAMLDEETYLAYRQKAMTLSPSLMFAHDDYEKLMHLTSSLNQLPVSSNTYTQILTNGREKFSKLLPALK
GASRHIHLEYYIFREDRISREIQRILIEKAKAGVEVRFLYDAVGSIHTSGTFFEEMKQAGVQVQAFFPVVLPLVSSKTNY
RNHRKIVVIDGTEAFTGGLNVGDEYLGEHSKFGFWRDTHLYVRGEGVSELQLIFLQDWYYMTGERLFTPFYLEPLETVRS
ASGGVQIVASGPDEPYETMKSIYFSLINAAKKSVYISSPYLIPDEDIMSALKTASLSGIDVRIVLPSYPDHKIVFYASRS
YYEELLLAGVKIYLYEKGFMHSKVIVVDDGLATIGTANMDFRSFHLNFEVNALLYNTNSVHQLKQDLYDDFEGSHELVLE
EFAKRSFFIKLVESLARMFSPLL
>Mature_503_residues
MLRRIQVLFSLILLAGLIAILSYYWSSQVIGLFSILVFLTTFSILLVILLENRNPQRTLIWAIVMIGFPVIGLFAYFVFG
QNYRRKRMFKEKAMLDEETYLAYRQKAMTLSPSLMFAHDDYEKLMHLTSSLNQLPVSSNTYTQILTNGREKFSKLLPALK
GASRHIHLEYYIFREDRISREIQRILIEKAKAGVEVRFLYDAVGSIHTSGTFFEEMKQAGVQVQAFFPVVLPLVSSKTNY
RNHRKIVVIDGTEAFTGGLNVGDEYLGEHSKFGFWRDTHLYVRGEGVSELQLIFLQDWYYMTGERLFTPFYLEPLETVRS
ASGGVQIVASGPDEPYETMKSIYFSLINAAKKSVYISSPYLIPDEDIMSALKTASLSGIDVRIVLPSYPDHKIVFYASRS
YYEELLLAGVKIYLYEKGFMHSKVIVVDDGLATIGTANMDFRSFHLNFEVNALLYNTNSVHQLKQDLYDDFEGSHELVLE
EFAKRSFFIKLVESLARMFSPLL

Specific function: Catalyzes the reversible phosphatidyl group transfer from one phosphatidylglycerol molecule to another to form cardiolipin (CL) (diphosphatidylglycerol) and glycerol [H]

COG id: COG1502

COG function: function code I; Phosphatidylserine/phosphatidylglycerophosphate/cardiolipin synthases and related enzymes

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 PLD phosphodiesterase domains [H]

Homologues:

Organism=Escherichia coli, GI1787502, Length=478, Percent_Identity=27.8242677824268, Blast_Score=203, Evalue=2e-53,
Organism=Escherichia coli, GI1787007, Length=357, Percent_Identity=28.0112044817927, Blast_Score=140, Evalue=2e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022924
- InterPro:   IPR001736 [H]

Pfam domain/function: PF00614 PLDc [H]

EC number: 2.7.8.-

Molecular weight: Translated: 57733; Mature: 57733

Theoretical pI: Translated: 7.23; Mature: 7.23

Prosite motif: PS50035 PLD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLRRIQVLFSLILLAGLIAILSYYWSSQVIGLFSILVFLTTFSILLVILLENRNPQRTLI
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHH
WAIVMIGFPVIGLFAYFVFGQNYRRKRMFKEKAMLDEETYLAYRQKAMTLSPSLMFAHDD
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCHHHHHHHHHHHHEECCCEEEECCH
YEKLMHLTSSLNQLPVSSNTYTQILTNGREKFSKLLPALKGASRHIHLEYYIFREDRISR
HHHHHHHHHHHHCCCCCCCHHHHHHHCCHHHHHHHHHHHCCCCCEEEEEEEEEECHHHHH
EIQRILIEKAKAGVEVRFLYDAVGSIHTSGTFFEEMKQAGVQVQAFFPVVLPLVSSKTNY
HHHHHHHHHHHCCCEEEEEEHHHHCCCCCCHHHHHHHHCCCEEEEHHHHHHHHHCCCCCC
RNHRKIVVIDGTEAFTGGLNVGDEYLGEHSKFGFWRDTHLYVRGEGVSELQLIFLQDWYY
CCCCEEEEEECCCHHCCCCCCCHHHCCCCCCCCCEECEEEEEECCCCCCEEEEEEECHHH
MTGERLFTPFYLEPLETVRSASGGVQIVASGPDEPYETMKSIYFSLINAAKKSVYISSPY
CCCCCEECCHHHCHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHEEEEECCC
LIPDEDIMSALKTASLSGIDVRIVLPSYPDHKIVFYASRSYYEELLLAGVKIYLYEKGFM
CCCCHHHHHHHHHCCCCCCEEEEEECCCCCCEEEEEECHHHHHHHHHCCEEEEEEECCCC
HSKVIVVDDGLATIGTANMDFRSFHLNFEVNALLYNTNSVHQLKQDLYDDFEGSHELVLE
CCEEEEEECCCCEEECCCCCEEEEEEEEEEEEEEECCCHHHHHHHHHHHHCCCCHHHHHH
EFAKRSFFIKLVESLARMFSPLL
HHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MLRRIQVLFSLILLAGLIAILSYYWSSQVIGLFSILVFLTTFSILLVILLENRNPQRTLI
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHH
WAIVMIGFPVIGLFAYFVFGQNYRRKRMFKEKAMLDEETYLAYRQKAMTLSPSLMFAHDD
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCHHHHHHHHHHHHEECCCEEEECCH
YEKLMHLTSSLNQLPVSSNTYTQILTNGREKFSKLLPALKGASRHIHLEYYIFREDRISR
HHHHHHHHHHHHCCCCCCCHHHHHHHCCHHHHHHHHHHHCCCCCEEEEEEEEEECHHHHH
EIQRILIEKAKAGVEVRFLYDAVGSIHTSGTFFEEMKQAGVQVQAFFPVVLPLVSSKTNY
HHHHHHHHHHHCCCEEEEEEHHHHCCCCCCHHHHHHHHCCCEEEEHHHHHHHHHCCCCCC
RNHRKIVVIDGTEAFTGGLNVGDEYLGEHSKFGFWRDTHLYVRGEGVSELQLIFLQDWYY
CCCCEEEEEECCCHHCCCCCCCHHHCCCCCCCCCEECEEEEEECCCCCCEEEEEEECHHH
MTGERLFTPFYLEPLETVRSASGGVQIVASGPDEPYETMKSIYFSLINAAKKSVYISSPY
CCCCCEECCHHHCHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHEEEEECCC
LIPDEDIMSALKTASLSGIDVRIVLPSYPDHKIVFYASRSYYEELLLAGVKIYLYEKGFM
CCCCHHHHHHHHHCCCCCCEEEEEECCCCCCEEEEEECHHHHHHHHHCCEEEEEEECCCC
HSKVIVVDDGLATIGTANMDFRSFHLNFEVNALLYNTNSVHQLKQDLYDDFEGSHELVLE
CCEEEEEECCCCEEECCCCCEEEEEEEEEEEEEEECCCHHHHHHHHHHHHCCCCHHHHHH
EFAKRSFFIKLVESLARMFSPLL
HHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12721629 [H]