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The map label for this gene is ruvB

Identifier: 229918407

GI number: 229918407

Start: 2658424

End: 2659425

Strand: Direct

Name: ruvB

Synonym: EAT1b_2692

Alternate gene names: 229918407

Gene position: 2658424-2659425 (Clockwise)

Preceding gene: 229918406

Following gene: 229918408

Centisome position: 88.62

GC content: 56.69

Gene sequence:

>1002_bases
ATGGACGAACGATTGTTGTCACAATCTCATCAGCAATATGAAGACTCTGAAGAGTGGAGCTTACGTCCGCAGCGCTTCGA
GCAATATATCGGGCAAGAAAAGGCGAAAGGCAATTTGTCCGTCTTCATTCAGGCCGCCAAACTTCGAAGTGAGACGCTCG
ACCACGTGCTCTTATACGGTCCCCCGGGACTCGGGAAGACGACGCTCGCCCAAATCATTGCCAACGAGATGGGTGTCGGA
ATCAAGACGACCGCCGGCCCGGCGATTGAACGACCGGGTGATTTGGCCGCAATCCTCTCGACGCTCGAACCGGGAGACGT
CTTGTTCATTGACGAGATTCACCGCTTGAGCCGGACGATTGAAGAGATCCTTTATCCGGCGATGGAAGATTACTGTCTCG
ACATCGTCTACGGGCAAGGCGAGATGGCACGTAGCGTCCGCATCGACCTACCACCGTTCACGCTCGTCGGGGCGACGACG
CGGGCCGGGATGTTGTCGGCACCTTTACGTGACCGTTTCGGCGTCACGTTGAAGCTCGAATATTATGAAACACATGAGTT
AGCGGCCATCGTCAGCCGGACGGCCCAGTTGTTCCGCCTCTCCATCTCACCTGAGGCGAGCGGTGCGATTGCGAAACGCT
CACGAGGGACGCCACGGATCGCGAACCGTCTCTTACGACGTGTCCGTGATTTCGCACAAGTGGCAGGAACGAAAGAAATC
GATCCCGTGCTCGCATCCGATGCGCTCGACCGGCTACACGTCGATGCGCTCGGACTCGATGAAGTCGACCATCGATTGCT
TCGGGCCATGGTGGAGCGGTTCGGAGGTGGACCGGTCGGGCTTGAGACGCTTGCCGCGACCATCGGTGAGGACGCTCAGA
CGATTGAGGATGTCTATGAGCCCTATTTATTGCAACAAGGGTTCTTGCAGCGCACGCCGCGAGGACGGATGATCACACAG
TTTGCCAAGTCGCATTTTGGTTATGAAGAGGAAGAGGAGTAA

Upstream 100 bases:

>100_bases
GAAATCGCAAAAGTCCGCAAAGCATTAAGCGGAGAAATCTTGACGACCGACGCGTACATAAAACGCGCGCTACAGCTGTT
ACTGAAATAAGGAGGAAGCT

Downstream 100 bases:

>100_bases
GGTATGCAAGACATTCAATTGAACGTAAACGATTATGATTTTGAATTGCCAGAAGAGTTGATCGCGCAAGTCCCATTGCT
TGATCGGACGAGCTCACGCT

Product: Holliday junction DNA helicase RuvB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 333; Mature: 333

Protein sequence:

>333_residues
MDERLLSQSHQQYEDSEEWSLRPQRFEQYIGQEKAKGNLSVFIQAAKLRSETLDHVLLYGPPGLGKTTLAQIIANEMGVG
IKTTAGPAIERPGDLAAILSTLEPGDVLFIDEIHRLSRTIEEILYPAMEDYCLDIVYGQGEMARSVRIDLPPFTLVGATT
RAGMLSAPLRDRFGVTLKLEYYETHELAAIVSRTAQLFRLSISPEASGAIAKRSRGTPRIANRLLRRVRDFAQVAGTKEI
DPVLASDALDRLHVDALGLDEVDHRLLRAMVERFGGGPVGLETLAATIGEDAQTIEDVYEPYLLQQGFLQRTPRGRMITQ
FAKSHFGYEEEEE

Sequences:

>Translated_333_residues
MDERLLSQSHQQYEDSEEWSLRPQRFEQYIGQEKAKGNLSVFIQAAKLRSETLDHVLLYGPPGLGKTTLAQIIANEMGVG
IKTTAGPAIERPGDLAAILSTLEPGDVLFIDEIHRLSRTIEEILYPAMEDYCLDIVYGQGEMARSVRIDLPPFTLVGATT
RAGMLSAPLRDRFGVTLKLEYYETHELAAIVSRTAQLFRLSISPEASGAIAKRSRGTPRIANRLLRRVRDFAQVAGTKEI
DPVLASDALDRLHVDALGLDEVDHRLLRAMVERFGGGPVGLETLAATIGEDAQTIEDVYEPYLLQQGFLQRTPRGRMITQ
FAKSHFGYEEEEE
>Mature_333_residues
MDERLLSQSHQQYEDSEEWSLRPQRFEQYIGQEKAKGNLSVFIQAAKLRSETLDHVLLYGPPGLGKTTLAQIIANEMGVG
IKTTAGPAIERPGDLAAILSTLEPGDVLFIDEIHRLSRTIEEILYPAMEDYCLDIVYGQGEMARSVRIDLPPFTLVGATT
RAGMLSAPLRDRFGVTLKLEYYETHELAAIVSRTAQLFRLSISPEASGAIAKRSRGTPRIANRLLRRVRDFAQVAGTKEI
DPVLASDALDRLHVDALGLDEVDHRLLRAMVERFGGGPVGLETLAATIGEDAQTIEDVYEPYLLQQGFLQRTPRGRMITQ
FAKSHFGYEEEEE

Specific function: The ruvA-ruvB complex in the presence of ATP renatures cruciform structure in supercoiled DNA with palindromic sequence, indicating that it may promote strand exchange reactions in homologous recombination. RuvAB is an helicase that mediates the Holliday

COG id: COG2255

COG function: function code L; Holliday junction resolvasome, helicase subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ruvB family

Homologues:

Organism=Escherichia coli, GI1788167, Length=330, Percent_Identity=59.0909090909091, Blast_Score=406, Evalue=1e-115,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RUVB_EXISA (C4L523)

Other databases:

- EMBL:   CP001615
- RefSeq:   YP_002887053.1
- GeneID:   7868896
- GenomeReviews:   CP001615_GR
- KEGG:   eat:EAT1b_2692
- OMA:   EKFDGGP
- ProtClustDB:   PRK00080
- HAMAP:   MF_00016
- InterPro:   IPR003593
- InterPro:   IPR004605
- InterPro:   IPR008823
- InterPro:   IPR008824
- InterPro:   IPR011991
- Gene3D:   G3DSA:1.10.10.10
- ProDom:   PD005323
- SMART:   SM00382
- TIGRFAMs:   TIGR00635

Pfam domain/function: PF05491 RuvB_C; PF05496 RuvB_N

EC number: =3.6.4.12

Molecular weight: Translated: 37097; Mature: 37097

Theoretical pI: Translated: 4.89; Mature: 4.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDERLLSQSHQQYEDSEEWSLRPQRFEQYIGQEKAKGNLSVFIQAAKLRSETLDHVLLYG
CCHHHHHHHHHHCCCCHHCCCCHHHHHHHHCHHCCCCCCEEEEEHHHHHHHHHCEEEEEC
PPGLGKTTLAQIIANEMGVGIKTTAGPAIERPGDLAAILSTLEPGDVLFIDEIHRLSRTI
CCCCCHHHHHHHHHHHHCCCEECCCCCCCCCCCHHHHHHHHCCCCCEEEHHHHHHHHHHH
EEILYPAMEDYCLDIVYGQGEMARSVRIDLPPFTLVGATTRAGMLSAPLRDRFGVTLKLE
HHHHHHHHHHHHHHEEECCCHHCCEEEECCCCEEEECCCHHCCHHCCCHHHHCCEEEEEE
YYETHELAAIVSRTAQLFRLSISPEASGAIAKRSRGTPRIANRLLRRVRDFAQVAGTKEI
EHHHHHHHHHHHHHHHHHEEECCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCC
DPVLASDALDRLHVDALGLDEVDHRLLRAMVERFGGGPVGLETLAATIGEDAQTIEDVYE
CHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCHHHHHHHHHH
PYLLQQGFLQRTPRGRMITQFAKSHFGYEEEEE
HHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MDERLLSQSHQQYEDSEEWSLRPQRFEQYIGQEKAKGNLSVFIQAAKLRSETLDHVLLYG
CCHHHHHHHHHHCCCCHHCCCCHHHHHHHHCHHCCCCCCEEEEEHHHHHHHHHCEEEEEC
PPGLGKTTLAQIIANEMGVGIKTTAGPAIERPGDLAAILSTLEPGDVLFIDEIHRLSRTI
CCCCCHHHHHHHHHHHHCCCEECCCCCCCCCCCHHHHHHHHCCCCCEEEHHHHHHHHHHH
EEILYPAMEDYCLDIVYGQGEMARSVRIDLPPFTLVGATTRAGMLSAPLRDRFGVTLKLE
HHHHHHHHHHHHHHEEECCCHHCCEEEECCCCEEEECCCHHCCHHCCCHHHHCCEEEEEE
YYETHELAAIVSRTAQLFRLSISPEASGAIAKRSRGTPRIANRLLRRVRDFAQVAGTKEI
EHHHHHHHHHHHHHHHHHEEECCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCC
DPVLASDALDRLHVDALGLDEVDHRLLRAMVERFGGGPVGLETLAATIGEDAQTIEDVYE
CHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCHHHHHHHHHH
PYLLQQGFLQRTPRGRMITQFAKSHFGYEEEEE
HHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA