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The map label for this gene is queA [H]

Identifier: 229918408

GI number: 229918408

Start: 2659429

End: 2660487

Strand: Direct

Name: queA [H]

Synonym: EAT1b_2693

Alternate gene names: 229918408

Gene position: 2659429-2660487 (Clockwise)

Preceding gene: 229918407

Following gene: 229918409

Centisome position: 88.65

GC content: 53.92

Gene sequence:

>1059_bases
ATGCAAGACATTCAATTGAACGTAAACGATTATGATTTTGAATTGCCAGAAGAGTTGATCGCGCAAGTCCCATTGCTTGA
TCGGACGAGCTCACGCTTGCTCGTATTAGACCGCGAGACAGGGGACATCGAACATCGTCATTTCCGTGACGTGCTCGATT
ACTTGAACGAAGGGGATGCGCTTGTCGTCAACGATTCGCGCGTCTTACCGGCACGTCTCTTTGGGGCGAAGCAAGAAACA
GGCGGGAAAGTCGAACTGCTCTTGTTGAAACAGACGGAAGATGATGTATGGGAAGCGCTCGTCAAACCGGCGAAAAAAGT
TCGCGTCGGGTCCATCGTCGAGTTCGGGGACGGTCTTCTCCGCGCGGAATGTGTCGAGGAACTCCCAGAAGGCGGACGTC
GTTTCAAGTTTGACTACGACGGCATCTTCTATGAGATTTTAGATGAGCTCGGAACGATGCCGCTCCCACCGTACATTCGC
GAACAACTCGATGACCAAGACCGTTATCAAACGGTCTATGCCCGTGAACGAGGCAGTGCGGCCGCTCCGACAGCCGGTCT
TCATTTCACCGAGGAACTGCTCGAAGCAGCCAAAGCGAAAGGGGTTCGGTTAATCCCGCTCACGCTTCACGTCGGCCTCG
GGACGTTCCGTCCGGTGACGGCTGATTCGATCGAGGAACATACGATGCATAGTGAATATTTCGAGTTGTCTGCTGAATCG
GCAGCTGCGCTTCGGGAGATTCGAGCGAATGGCGGTCGCATCTTTGCGGTCGGCACGACATCGACGCGGACACTCGAGAC
GATCATGCGAGACCATGGGGACTTCGTCGAGTCATCGGGCTGGACGGATATCTTTATCTACCCGGGTGTCGAGTTGAAGG
CGATCGATGGATTGATCACGAACTTCCACTTGCCTAAATCGACGCTCGTTATGCTCGTCTCGGCGTTCGCGACGCGCGAC
ATCATCTTACACGCTTATCGGACTGCGGTCGCAGAACGATACCGCTTCTTCAGCTTTGGAGACGCGATGCTCATCACGAG
GAAGGAAGTAACAAAATGA

Upstream 100 bases:

>100_bases
TTTATTGCAACAAGGGTTCTTGCAGCGCACGCCGCGAGGACGGATGATCACACAGTTTGCCAAGTCGCATTTTGGTTATG
AAGAGGAAGAGGAGTAAGGT

Downstream 100 bases:

>100_bases
CAAAACATGCAGTCACATACGAACACATCCACACATGCAAACAGACCGGAGCGCGGCACGGAATCGTCCATACGCCGCAC
GGGTCGTTCGAGACACCGGT

Product: S-adenosylmethionine:tRNA ribosyltransferase-isomerase

Products: NA

Alternate protein names: Queuosine biosynthesis protein queA [H]

Number of amino acids: Translated: 352; Mature: 352

Protein sequence:

>352_residues
MQDIQLNVNDYDFELPEELIAQVPLLDRTSSRLLVLDRETGDIEHRHFRDVLDYLNEGDALVVNDSRVLPARLFGAKQET
GGKVELLLLKQTEDDVWEALVKPAKKVRVGSIVEFGDGLLRAECVEELPEGGRRFKFDYDGIFYEILDELGTMPLPPYIR
EQLDDQDRYQTVYARERGSAAAPTAGLHFTEELLEAAKAKGVRLIPLTLHVGLGTFRPVTADSIEEHTMHSEYFELSAES
AAALREIRANGGRIFAVGTTSTRTLETIMRDHGDFVESSGWTDIFIYPGVELKAIDGLITNFHLPKSTLVMLVSAFATRD
IILHAYRTAVAERYRFFSFGDAMLITRKEVTK

Sequences:

>Translated_352_residues
MQDIQLNVNDYDFELPEELIAQVPLLDRTSSRLLVLDRETGDIEHRHFRDVLDYLNEGDALVVNDSRVLPARLFGAKQET
GGKVELLLLKQTEDDVWEALVKPAKKVRVGSIVEFGDGLLRAECVEELPEGGRRFKFDYDGIFYEILDELGTMPLPPYIR
EQLDDQDRYQTVYARERGSAAAPTAGLHFTEELLEAAKAKGVRLIPLTLHVGLGTFRPVTADSIEEHTMHSEYFELSAES
AAALREIRANGGRIFAVGTTSTRTLETIMRDHGDFVESSGWTDIFIYPGVELKAIDGLITNFHLPKSTLVMLVSAFATRD
IILHAYRTAVAERYRFFSFGDAMLITRKEVTK
>Mature_352_residues
MQDIQLNVNDYDFELPEELIAQVPLLDRTSSRLLVLDRETGDIEHRHFRDVLDYLNEGDALVVNDSRVLPARLFGAKQET
GGKVELLLLKQTEDDVWEALVKPAKKVRVGSIVEFGDGLLRAECVEELPEGGRRFKFDYDGIFYEILDELGTMPLPPYIR
EQLDDQDRYQTVYARERGSAAAPTAGLHFTEELLEAAKAKGVRLIPLTLHVGLGTFRPVTADSIEEHTMHSEYFELSAES
AAALREIRANGGRIFAVGTTSTRTLETIMRDHGDFVESSGWTDIFIYPGVELKAIDGLITNFHLPKSTLVMLVSAFATRD
IILHAYRTAVAERYRFFSFGDAMLITRKEVTK

Specific function: Transfers and isomerizes the ribose moiety from AdoMet to the 7-aminomethyl group of 7-deazaguanine (preQ1-tRNA) to give epoxyqueuosine (oQ-tRNA) [H]

COG id: COG0809

COG function: function code J; S-adenosylmethionine:tRNA-ribosyltransferase-isomerase (queuine synthetase)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queA family [H]

Homologues:

Organism=Escherichia coli, GI1786606, Length=345, Percent_Identity=47.8260869565217, Blast_Score=305, Evalue=3e-84,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003699 [H]

Pfam domain/function: PF02547 Queuosine_synth [H]

EC number: 5.-.-.- [C]

Molecular weight: Translated: 39670; Mature: 39670

Theoretical pI: Translated: 4.71; Mature: 4.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQDIQLNVNDYDFELPEELIAQVPLLDRTSSRLLVLDRETGDIEHRHFRDVLDYLNEGDA
CCCEEECCCCCCCCCCHHHHHHCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHCCCCE
LVVNDSRVLPARLFGAKQETGGKVELLLLKQTEDDVWEALVKPAKKVRVGSIVEFGDGLL
EEEECCCCCHHHHHCCCCCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHCCHHHHCCCHH
RAECVEELPEGGRRFKFDYDGIFYEILDELGTMPLPPYIREQLDDQDRYQTVYARERGSA
HHHHHHHCCCCCCEEEEECCHHHHHHHHHHCCCCCCHHHHHHCCCHHHHHHHHHHCCCCC
AAPTAGLHFTEELLEAAKAKGVRLIPLTLHVGLGTFRPVTADSIEEHTMHSEYFELSAES
CCCCCCHHHHHHHHHHHHHCCEEEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHCHHH
AAALREIRANGGRIFAVGTTSTRTLETIMRDHGDFVESSGWTDIFIYPGVELKAIDGLIT
HHHHHHHHCCCCEEEEEECCCHHHHHHHHHHCCCHHHCCCCEEEEEECCCEEEEHHHHHH
NFHLPKSTLVMLVSAFATRDIILHAYRTAVAERYRFFSFGDAMLITRKEVTK
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCEEEEECHHHCC
>Mature Secondary Structure
MQDIQLNVNDYDFELPEELIAQVPLLDRTSSRLLVLDRETGDIEHRHFRDVLDYLNEGDA
CCCEEECCCCCCCCCCHHHHHHCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHCCCCE
LVVNDSRVLPARLFGAKQETGGKVELLLLKQTEDDVWEALVKPAKKVRVGSIVEFGDGLL
EEEECCCCCHHHHHCCCCCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHCCHHHHCCCHH
RAECVEELPEGGRRFKFDYDGIFYEILDELGTMPLPPYIREQLDDQDRYQTVYARERGSA
HHHHHHHCCCCCCEEEEECCHHHHHHHHHHCCCCCCHHHHHHCCCHHHHHHHHHHCCCCC
AAPTAGLHFTEELLEAAKAKGVRLIPLTLHVGLGTFRPVTADSIEEHTMHSEYFELSAES
CCCCCCHHHHHHHHHHHHHCCEEEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHCHHH
AAALREIRANGGRIFAVGTTSTRTLETIMRDHGDFVESSGWTDIFIYPGVELKAIDGLIT
HHHHHHHHCCCCEEEEEECCCHHHHHHHHHHCCCHHHCCCCEEEEEECCCEEEEHHHHHH
NFHLPKSTLVMLVSAFATRDIILHAYRTAVAERYRFFSFGDAMLITRKEVTK
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCEEEEECHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Isomerases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA