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The map label for this gene is ruvA

Identifier: 229918406

GI number: 229918406

Start: 2657826

End: 2658413

Strand: Direct

Name: ruvA

Synonym: EAT1b_2691

Alternate gene names: 229918406

Gene position: 2657826-2658413 (Clockwise)

Preceding gene: 229918405

Following gene: 229918407

Centisome position: 88.6

GC content: 50.85

Gene sequence:

>588_bases
GTGATTGAATTCGTAAAAGGACCCGTCGCCTATGTGTGTGCGGAATATATAACGTTAGATGTCTCGGGCGTCGGCTACAA
AGTACATGTGCCTAACCCGTTTTTTTATCGTGAACAAGACGAACATGTAATCGTGTTTACGCATCATTATGTGCGTGAAG
ACCAACAGACGTTATACGGTTTCCGTTCACGTCGCGAGCGTGAGCTGTTCAATAAACTCCTCAGCGTGAGCGGGATTGGA
CCAAAGGGTGCGCTTGCCATCGTCGCGTCCGGCGATATCGACGCGTTGATCGATGCGATTGAAACCGAGAACGAGAAGTA
TTTGACGAAGTTCCCGGGAGTCGGGAAGAAGACGGCCAAACAGATGGCACTCGATTTGAAAGGGAAGTTATCCGAACTCG
CACCGGATTACGTGCCGAACGAAGGGTTGTTCGCACAAGGTGCATCTGAACTCGATGAGGCGTGTGAGGCGCTCGTCGCG
CTCGGCTACTCCGAACGGGAAATCGCAAAAGTCCGCAAAGCATTAAGCGGAGAAATCTTGACGACCGACGCGTACATAAA
ACGCGCGCTACAGCTGTTACTGAAATAA

Upstream 100 bases:

>100_bases
GCGATAATTGCTAGAGGACAGGTAAACGCCTGTCCTTTTTTCGTGTCGCCCATTATGGTACAATAAATGCAGTTTTTCCG
ACCGAAAAGGAGAGTACACC

Downstream 100 bases:

>100_bases
GGAGGAAGCTATGGACGAACGATTGTTGTCACAATCTCATCAGCAATATGAAGACTCTGAAGAGTGGAGCTTACGTCCGC
AGCGCTTCGAGCAATATATC

Product: Holliday junction DNA helicase RuvA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 195; Mature: 195

Protein sequence:

>195_residues
MIEFVKGPVAYVCAEYITLDVSGVGYKVHVPNPFFYREQDEHVIVFTHHYVREDQQTLYGFRSRRERELFNKLLSVSGIG
PKGALAIVASGDIDALIDAIETENEKYLTKFPGVGKKTAKQMALDLKGKLSELAPDYVPNEGLFAQGASELDEACEALVA
LGYSEREIAKVRKALSGEILTTDAYIKRALQLLLK

Sequences:

>Translated_195_residues
MIEFVKGPVAYVCAEYITLDVSGVGYKVHVPNPFFYREQDEHVIVFTHHYVREDQQTLYGFRSRRERELFNKLLSVSGIG
PKGALAIVASGDIDALIDAIETENEKYLTKFPGVGKKTAKQMALDLKGKLSELAPDYVPNEGLFAQGASELDEACEALVA
LGYSEREIAKVRKALSGEILTTDAYIKRALQLLLK
>Mature_195_residues
MIEFVKGPVAYVCAEYITLDVSGVGYKVHVPNPFFYREQDEHVIVFTHHYVREDQQTLYGFRSRRERELFNKLLSVSGIG
PKGALAIVASGDIDALIDAIETENEKYLTKFPGVGKKTAKQMALDLKGKLSELAPDYVPNEGLFAQGASELDEACEALVA
LGYSEREIAKVRKALSGEILTTDAYIKRALQLLLK

Specific function: The ruvA-ruvB complex in the presence of ATP renatures cruciform structure in supercoiled DNA with palindromic sequence, indicating that it may promote strand exchange reactions in homologous recombination. RuvAB is an helicase that mediates the Holliday

COG id: COG0632

COG function: function code L; Holliday junction resolvasome, DNA-binding subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ruvA family

Homologues:

Organism=Escherichia coli, GI1788168, Length=203, Percent_Identity=35.4679802955665, Blast_Score=123, Evalue=9e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RUVA_EXISA (C4L522)

Other databases:

- EMBL:   CP001615
- RefSeq:   YP_002887052.1
- GeneID:   7868895
- GenomeReviews:   CP001615_GR
- KEGG:   eat:EAT1b_2691
- OMA:   LFGFAQK
- ProtClustDB:   PRK00116
- HAMAP:   MF_00031
- InterPro:   IPR011114
- InterPro:   IPR013849
- InterPro:   IPR003583
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR000085
- InterPro:   IPR010994
- Gene3D:   G3DSA:2.40.50.140
- SMART:   SM00278
- TIGRFAMs:   TIGR00084

Pfam domain/function: PF07499 RuvA_C; PF01330 RuvA_N; SSF50249 Nucleic_acid_OB; SSF47781 RuvA_2_like; SSF46929 RuvA_C-like

EC number: =3.6.4.12

Molecular weight: Translated: 21672; Mature: 21672

Theoretical pI: Translated: 5.72; Mature: 5.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIEFVKGPVAYVCAEYITLDVSGVGYKVHVPNPFFYREQDEHVIVFTHHYVREDQQTLYG
CCCCCCCCHHHHHHHHHEEEECCCEEEEECCCCCEECCCCCEEEEEEHHHHHHHHHHHHH
FRSRRERELFNKLLSVSGIGPKGALAIVASGDIDALIDAIETENEKYLTKFPGVGKKTAK
HHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCHHHHHHHHHCCCCHHHHHCCCCCHHHHH
QMALDLKGKLSELAPDYVPNEGLFAQGASELDEACEALVALGYSEREIAKVRKALSGEIL
HHHHHHHHHHHHHCCCCCCCCCCHHCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCEE
TTDAYIKRALQLLLK
EHHHHHHHHHHHHCC
>Mature Secondary Structure
MIEFVKGPVAYVCAEYITLDVSGVGYKVHVPNPFFYREQDEHVIVFTHHYVREDQQTLYG
CCCCCCCCHHHHHHHHHEEEECCCEEEEECCCCCEECCCCCEEEEEEHHHHHHHHHHHHH
FRSRRERELFNKLLSVSGIGPKGALAIVASGDIDALIDAIETENEKYLTKFPGVGKKTAK
HHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCHHHHHHHHHCCCCHHHHHCCCCCHHHHH
QMALDLKGKLSELAPDYVPNEGLFAQGASELDEACEALVALGYSEREIAKVRKALSGEIL
HHHHHHHHHHHHHCCCCCCCCCCHHCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCEE
TTDAYIKRALQLLLK
EHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA