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The map label for this gene is epsE [H]

Identifier: 229918375

GI number: 229918375

Start: 2630507

End: 2632177

Strand: Direct

Name: epsE [H]

Synonym: EAT1b_2660

Alternate gene names: 229918375

Gene position: 2630507-2632177 (Clockwise)

Preceding gene: 229918374

Following gene: 229918376

Centisome position: 87.69

GC content: 50.75

Gene sequence:

>1671_bases
GTGAGACGAACAAAACGAAGATTAGGTGAGATGCTGATCGAGGCTGCGCTTATTACGACGAATCAGCTCGATGAGGCGCT
TGAACAGAAGCGTCCCGGAGAAAAACTAGGGGACGCCTTAATTCGACTAAACCATATTACCGAGACACAGTTGATTCAAA
TGATTCACGAACAGTTGCATGTGCCAATCATTGAGTTGTACTCCTATGACATCAATGTGACGGTCACGAAGCTCGTACCG
AAAGCATTGGCACAAAAGCACGATATCATGCCATTTGAATTGAATGGGAATACGCTTCATGTCGCAACGGCCGACCCACT
CGATCTCATTGCCATTGATGACGTCCGTCTTCAGACCGGGATGAACATCGAAATCGGCATCGCGACGAGAGAGCAGATTC
GAAAGACGATCAGTCGATACTACGAGATGGATCACTCGCTCGTCGAAATTTTAAAAGAGGATGCTCCCGAAATCGAACGA
CAAGAAACAATCAGTCGAGACGATGCACCGATTATTCGACTCGTGAACCAGTTGTTCTTAAGTGCCATCGACCAACGCGC
TTCGGATATTCACTTCGACCCGCATGAGAAACAGCTCCACGTCCGTTTTCGAATTGATGGGGACTTGCGCACCGAGACGG
TCTATCCGAAGAAGATTCAAAGCGTCATGCTGACGCGTCTCAAAGTCATGAGTAACCTCGACATCACAGAAAGTCGCCTA
CCACAAGATGGGCGAATCAAGTACACGCTCCGCAATCGCGCCTATGATTTCCGCGTCTCGACGCTCCCGACGATGTATGG
GGAGAAAATCGTTATTCGGGTCCTCGATTCATCGCAAGGACTTACCGATTTGACGAAACTCGGATTCGGCGAACGAAATG
AACAGGCGTACCGTGACATGCTGAAACGTCCGAACGGGATTATTTTGATCACCGGTCCGACCGGTTCAGGGAAGTCCTCG
ACATTGTACGCTTCGTTGAAAGAACTGAATGATGAGACGAAAAACATCATTACGGTAGAAGACCCGGTCGAGTATCAACT
TGACGGCATCAACCAAGTTCAGGTCAACTCGAAAGTCGGCTTGACGTTCGCGTCAGGGCTTCGTTCGATTTTACGTCAAG
ACCCGAACATCGTCATGGTAGGAGAGATTCGGGATACGGAGACGGCTGAGATTGCCATTCGTGCCTCGCTCACGGGTCAC
CTTGTTTTGTCAACGCTACATACGAATTCAGCGATCAGCTCCATCACCCGTCTCGTCGATATGGGTGTCGAACCGTTCCT
TGTCGCCGCTTCGACGACGGGACTTGTGGCACAACGACTCGTTCGACGCGTCTGTCGTGACTGTGGTCAAGTCGAACCGG
TATCGAAGAGCGAACGTAGCCTGTTCGACGCAGAGGGTGTACACATCGACCACATCGTTCGGGGGAAAGGCTGTGCGTCA
TGCAACTTCACAGGGTATCGTGGGCGCCTCGCCATTCACGAAGTCGTCCCAATCGATGAAGGCTTGCGCCGGATGATCAT
GAACCAGGCGACCGAGTCTGAGATGGTCGACTATGCCAAGTCACAAGGAGCGACATTCCTCCTTGAGGACGGGTTAGAAA
AAGTCGCCGCCGGTCTGACGACGACTGAAGAAATATTACGAACGGTCATTACGGAGAATGCTTATGATTAA

Upstream 100 bases:

>100_bases
GTTGTATTGTTGATTGGAGGGGTGGCTGCGAGCGTCTTTGTCGAAGAACGGAATGACAAGGCCGAGCAGGAGGAGGCTCC
TCAAAGGATGGTGAAGTAAC

Downstream 100 bases:

>100_bases
TCAATCGATTGAAACCATTTTGACGAATGCGGTCGAGAAACGTGCCTCGGATATTCACCTGACAGCCGGTTCCCCACCGA
TTATTCGAGTAGATGGGAGT

Product: type II secretion system protein E

Products: NA

Alternate protein names: Cholera toxin secretion protein epsE; Type II traffic warden ATPase [H]

Number of amino acids: Translated: 556; Mature: 556

Protein sequence:

>556_residues
MRRTKRRLGEMLIEAALITTNQLDEALEQKRPGEKLGDALIRLNHITETQLIQMIHEQLHVPIIELYSYDINVTVTKLVP
KALAQKHDIMPFELNGNTLHVATADPLDLIAIDDVRLQTGMNIEIGIATREQIRKTISRYYEMDHSLVEILKEDAPEIER
QETISRDDAPIIRLVNQLFLSAIDQRASDIHFDPHEKQLHVRFRIDGDLRTETVYPKKIQSVMLTRLKVMSNLDITESRL
PQDGRIKYTLRNRAYDFRVSTLPTMYGEKIVIRVLDSSQGLTDLTKLGFGERNEQAYRDMLKRPNGIILITGPTGSGKSS
TLYASLKELNDETKNIITVEDPVEYQLDGINQVQVNSKVGLTFASGLRSILRQDPNIVMVGEIRDTETAEIAIRASLTGH
LVLSTLHTNSAISSITRLVDMGVEPFLVAASTTGLVAQRLVRRVCRDCGQVEPVSKSERSLFDAEGVHIDHIVRGKGCAS
CNFTGYRGRLAIHEVVPIDEGLRRMIMNQATESEMVDYAKSQGATFLLEDGLEKVAAGLTTTEEILRTVITENAYD

Sequences:

>Translated_556_residues
MRRTKRRLGEMLIEAALITTNQLDEALEQKRPGEKLGDALIRLNHITETQLIQMIHEQLHVPIIELYSYDINVTVTKLVP
KALAQKHDIMPFELNGNTLHVATADPLDLIAIDDVRLQTGMNIEIGIATREQIRKTISRYYEMDHSLVEILKEDAPEIER
QETISRDDAPIIRLVNQLFLSAIDQRASDIHFDPHEKQLHVRFRIDGDLRTETVYPKKIQSVMLTRLKVMSNLDITESRL
PQDGRIKYTLRNRAYDFRVSTLPTMYGEKIVIRVLDSSQGLTDLTKLGFGERNEQAYRDMLKRPNGIILITGPTGSGKSS
TLYASLKELNDETKNIITVEDPVEYQLDGINQVQVNSKVGLTFASGLRSILRQDPNIVMVGEIRDTETAEIAIRASLTGH
LVLSTLHTNSAISSITRLVDMGVEPFLVAASTTGLVAQRLVRRVCRDCGQVEPVSKSERSLFDAEGVHIDHIVRGKGCAS
CNFTGYRGRLAIHEVVPIDEGLRRMIMNQATESEMVDYAKSQGATFLLEDGLEKVAAGLTTTEEILRTVITENAYD
>Mature_556_residues
MRRTKRRLGEMLIEAALITTNQLDEALEQKRPGEKLGDALIRLNHITETQLIQMIHEQLHVPIIELYSYDINVTVTKLVP
KALAQKHDIMPFELNGNTLHVATADPLDLIAIDDVRLQTGMNIEIGIATREQIRKTISRYYEMDHSLVEILKEDAPEIER
QETISRDDAPIIRLVNQLFLSAIDQRASDIHFDPHEKQLHVRFRIDGDLRTETVYPKKIQSVMLTRLKVMSNLDITESRL
PQDGRIKYTLRNRAYDFRVSTLPTMYGEKIVIRVLDSSQGLTDLTKLGFGERNEQAYRDMLKRPNGIILITGPTGSGKSS
TLYASLKELNDETKNIITVEDPVEYQLDGINQVQVNSKVGLTFASGLRSILRQDPNIVMVGEIRDTETAEIAIRASLTGH
LVLSTLHTNSAISSITRLVDMGVEPFLVAASTTGLVAQRLVRRVCRDCGQVEPVSKSERSLFDAEGVHIDHIVRGKGCAS
CNFTGYRGRLAIHEVVPIDEGLRRMIMNQATESEMVDYAKSQGATFLLEDGLEKVAAGLTTTEEILRTVITENAYD

Specific function: Required for secretion of cholera toxin through the outer membrane [H]

COG id: COG2804

COG function: function code NU; Type II secretory pathway, ATPase PulE/Tfp pilus assembly pathway, ATPase PilB

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI1789723, Length=379, Percent_Identity=50.9234828496042, Blast_Score=393, Evalue=1e-110,
Organism=Escherichia coli, GI1786296, Length=453, Percent_Identity=39.0728476821192, Blast_Score=314, Evalue=1e-86,
Organism=Escherichia coli, GI87082188, Length=244, Percent_Identity=34.0163934426229, Blast_Score=124, Evalue=1e-29,
Organism=Escherichia coli, GI1786781, Length=139, Percent_Identity=27.3381294964029, Blast_Score=63, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR013369
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 62478; Mature: 62478

Theoretical pI: Translated: 5.93; Mature: 5.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRTKRRLGEMLIEAALITTNQLDEALEQKRPGEKLGDALIRLNHITETQLIQMIHEQLH
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHC
VPIIELYSYDINVTVTKLVPKALAQKHDIMPFELNGNTLHVATADPLDLIAIDDVRLQTG
CCEEEEEECCCCEEHHHHHHHHHHHHCCCCEEEECCCEEEEEECCCCEEEEECCEEEECC
MNIEIGIATREQIRKTISRYYEMDHSLVEILKEDAPEIERQETISRDDAPIIRLVNQLFL
CEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCHHHHHHHHHHH
SAIDQRASDIHFDPHEKQLHVRFRIDGDLRTETVYPKKIQSVMLTRLKVMSNLDITESRL
HHHHHHHHCCCCCCCCCEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHC
PQDGRIKYTLRNRAYDFRVSTLPTMYGEKIVIRVLDSSQGLTDLTKLGFGERNEQAYRDM
CCCCCEEEEEECCCEEEEECCCCCCCCCEEEEEEECCCCCHHHHHHCCCCCCCHHHHHHH
LKRPNGIILITGPTGSGKSSTLYASLKELNDETKNIITVEDPVEYQLDGINQVQVNSKVG
HHCCCCEEEEECCCCCCCCHHHHHHHHHHCCCCCCEEEECCCCCEEECCCCEEEECCCCC
LTFASGLRSILRQDPNIVMVGEIRDTETAEIAIRASLTGHLVLSTLHTNSAISSITRLVD
HHHHHHHHHHHHCCCCEEEEECCCCCCCEEEEEEEECCCEEEEEEHHHHHHHHHHHHHHH
MGVEPFLVAASTTGLVAQRLVRRVCRDCGQVEPVSKSERSLFDAEGVHIDHIVRGKGCAS
CCCCCEEEEECHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHCCCCCEEEEEECCCCCCC
CNFTGYRGRLAIHEVVPIDEGLRRMIMNQATESEMVDYAKSQGATFLLEDGLEKVAAGLT
CCCCCCCCCEEEEEECCHHHHHHHHHHHHCHHHHHHHHHHHCCCEEEHHHHHHHHHHCCC
TTEEILRTVITENAYD
CHHHHHHHHHHCCCCC
>Mature Secondary Structure
MRRTKRRLGEMLIEAALITTNQLDEALEQKRPGEKLGDALIRLNHITETQLIQMIHEQLH
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHC
VPIIELYSYDINVTVTKLVPKALAQKHDIMPFELNGNTLHVATADPLDLIAIDDVRLQTG
CCEEEEEECCCCEEHHHHHHHHHHHHCCCCEEEECCCEEEEEECCCCEEEEECCEEEECC
MNIEIGIATREQIRKTISRYYEMDHSLVEILKEDAPEIERQETISRDDAPIIRLVNQLFL
CEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCHHHHHHHHHHH
SAIDQRASDIHFDPHEKQLHVRFRIDGDLRTETVYPKKIQSVMLTRLKVMSNLDITESRL
HHHHHHHHCCCCCCCCCEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHC
PQDGRIKYTLRNRAYDFRVSTLPTMYGEKIVIRVLDSSQGLTDLTKLGFGERNEQAYRDM
CCCCCEEEEEECCCEEEEECCCCCCCCCEEEEEEECCCCCHHHHHHCCCCCCCHHHHHHH
LKRPNGIILITGPTGSGKSSTLYASLKELNDETKNIITVEDPVEYQLDGINQVQVNSKVG
HHCCCCEEEEECCCCCCCCHHHHHHHHHHCCCCCCEEEECCCCCEEECCCCEEEECCCCC
LTFASGLRSILRQDPNIVMVGEIRDTETAEIAIRASLTGHLVLSTLHTNSAISSITRLVD
HHHHHHHHHHHHCCCCEEEEECCCCCCCEEEEEEEECCCEEEEEEHHHHHHHHHHHHHHH
MGVEPFLVAASTTGLVAQRLVRRVCRDCGQVEPVSKSERSLFDAEGVHIDHIVRGKGCAS
CCCCCEEEEECHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHCCCCCEEEEEECCCCCCC
CNFTGYRGRLAIHEVVPIDEGLRRMIMNQATESEMVDYAKSQGATFLLEDGLEKVAAGLT
CCCCCCCCCEEEEEECCHHHHHHHHHHHHCHHHHHHHHHHHCCCEEEHHHHHHHHHHCCC
TTEEILRTVITENAYD
CHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8423007; 10952301 [H]