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The map label for this gene is pilT [H]

Identifier: 229918376

GI number: 229918376

Start: 2632170

End: 2633207

Strand: Direct

Name: pilT [H]

Synonym: EAT1b_2661

Alternate gene names: 229918376

Gene position: 2632170-2633207 (Clockwise)

Preceding gene: 229918375

Following gene: 229918377

Centisome position: 87.74

GC content: 52.7

Gene sequence:

>1038_bases
ATGATTAATCAATCGATTGAAACCATTTTGACGAATGCGGTCGAGAAACGTGCCTCGGATATTCACCTGACAGCCGGTTC
CCCACCGATTATTCGAGTAGATGGGAGTCTCATTACGATGGGCGAGGAGAAGGTATTGCCGCACCATCTTGAAGGATATT
TAAAAGAAATTATGACCGATGATATGCGCGAACGTCTCGAGCATGATCGGGATCTCGATTTTTCGTTCGGTGTCCCACGT
GTTTCCCGTTTTCGGGTCAACGCCTACTATCAACGAGGCACGATTGCCATCGCATTCCGGTCCATTTCCGGGGAAATCCC
GACATTGAACGAGCTCAATCTGCCGACGGTGCTGAAGCGTTTGATTGAGAAACCACATGGTCTCATCTTGGTCACGGGTC
CGACGGGTTCAGGGAAATCGACGACGCTCGCGGCGATGATTCATGAGATTAACCGCACGCAGCGAAAGCGGATCATCACG
CTAGAAGATCCGATCGAGTATTTGCATTCCCATGATCAAAGTATCGTCGACCAACGGGAAATCGGCTCTGACGTGATGAA
ATTTGTGAGTGGTCTACGGGCTGCCTTACGTCAAGACCCGGACGTCATTTTGCTTGGGGAGATGCGGGACTTGGAGACCA
TCTCGACGGCGATGACCGCCGCAGAGACAGGGCACCTCGTCATGGCGACGCTCCATACATCGAGTGCGATGTCGACGGTG
AGTCGAATCATCGACGCATTCCCAGCAGAGCAACAAGACCAAATCCGGACACAACTCGCCAACGTCCTGCTTGGCGTCGT
GAGTCAACGGTTATTTCCACGGATTGATGTGCCGAAAGGCCGCGTCGCCGCCATGGAAATCATGTTGGGGAATCCGGCAG
TTGCCAACTTGATTCGAAATGAAAAAGAGTTCCAGTTGCCGACCGTCATCCAGACGAGTCGCCAACACGGGATGCAGTCG
ATGGATGCCGCCATCGAACAACTCGTTCGACAAGGGATTATCAACCCGAACGCCGTCCCGACAGAAGAGATGAGGTGA

Upstream 100 bases:

>100_bases
CAAGGAGCGACATTCCTCCTTGAGGACGGGTTAGAAAAAGTCGCCGCCGGTCTGACGACGACTGAAGAAATATTACGAAC
GGTCATTACGGAGAATGCTT

Downstream 100 bases:

>100_bases
TGAGTGATGGCGATATTTCAGTATGAAGGAAAACTCCTGAACGGGAAGCGTAAGAAAGGTCGCATCACGGCCGTTTCGCT
TCGGGAAGCGAAAGAAAAGT

Product: twitching motility protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 345; Mature: 345

Protein sequence:

>345_residues
MINQSIETILTNAVEKRASDIHLTAGSPPIIRVDGSLITMGEEKVLPHHLEGYLKEIMTDDMRERLEHDRDLDFSFGVPR
VSRFRVNAYYQRGTIAIAFRSISGEIPTLNELNLPTVLKRLIEKPHGLILVTGPTGSGKSTTLAAMIHEINRTQRKRIIT
LEDPIEYLHSHDQSIVDQREIGSDVMKFVSGLRAALRQDPDVILLGEMRDLETISTAMTAAETGHLVMATLHTSSAMSTV
SRIIDAFPAEQQDQIRTQLANVLLGVVSQRLFPRIDVPKGRVAAMEIMLGNPAVANLIRNEKEFQLPTVIQTSRQHGMQS
MDAAIEQLVRQGIINPNAVPTEEMR

Sequences:

>Translated_345_residues
MINQSIETILTNAVEKRASDIHLTAGSPPIIRVDGSLITMGEEKVLPHHLEGYLKEIMTDDMRERLEHDRDLDFSFGVPR
VSRFRVNAYYQRGTIAIAFRSISGEIPTLNELNLPTVLKRLIEKPHGLILVTGPTGSGKSTTLAAMIHEINRTQRKRIIT
LEDPIEYLHSHDQSIVDQREIGSDVMKFVSGLRAALRQDPDVILLGEMRDLETISTAMTAAETGHLVMATLHTSSAMSTV
SRIIDAFPAEQQDQIRTQLANVLLGVVSQRLFPRIDVPKGRVAAMEIMLGNPAVANLIRNEKEFQLPTVIQTSRQHGMQS
MDAAIEQLVRQGIINPNAVPTEEMR
>Mature_345_residues
MINQSIETILTNAVEKRASDIHLTAGSPPIIRVDGSLITMGEEKVLPHHLEGYLKEIMTDDMRERLEHDRDLDFSFGVPR
VSRFRVNAYYQRGTIAIAFRSISGEIPTLNELNLPTVLKRLIEKPHGLILVTGPTGSGKSTTLAAMIHEINRTQRKRIIT
LEDPIEYLHSHDQSIVDQREIGSDVMKFVSGLRAALRQDPDVILLGEMRDLETISTAMTAAETGHLVMATLHTSSAMSTV
SRIIDAFPAEQQDQIRTQLANVLLGVVSQRLFPRIDVPKGRVAAMEIMLGNPAVANLIRNEKEFQLPTVIQTSRQHGMQS
MDAAIEQLVRQGIINPNAVPTEEMR

Specific function: May be involved in pilus retraction [H]

COG id: COG2805

COG function: function code NU; Tfp pilus assembly protein, pilus retraction ATPase PilT

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI87082188, Length=331, Percent_Identity=46.2235649546828, Blast_Score=284, Evalue=6e-78,
Organism=Escherichia coli, GI1789723, Length=294, Percent_Identity=36.734693877551, Blast_Score=141, Evalue=6e-35,
Organism=Escherichia coli, GI1786296, Length=286, Percent_Identity=34.965034965035, Blast_Score=123, Evalue=2e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR006321
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 38432; Mature: 38432

Theoretical pI: Translated: 6.45; Mature: 6.45

Prosite motif: PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MINQSIETILTNAVEKRASDIHLTAGSPPIIRVDGSLITMGEEKVLPHHLEGYLKEIMTD
CCCHHHHHHHHHHHHHHCCCEEEECCCCCEEEECCCEEECCCCCCCHHHHHHHHHHHHHH
DMRERLEHDRDLDFSFGVPRVSRFRVNAYYQRGTIAIAFRSISGEIPTLNELNLPTVLKR
HHHHHHHCCCCCCCCCCCCCHHHEEEEEEEECCEEEEEEEECCCCCCCCCCCCHHHHHHH
LIEKPHGLILVTGPTGSGKSTTLAAMIHEINRTQRKRIITLEDPIEYLHSHDQSIVDQRE
HHHCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHEEEEEHHHHHHHHCCCHHHHHHHH
IGSDVMKFVSGLRAALRQDPDVILLGEMRDLETISTAMTAAETGHLVMATLHTSSAMSTV
HHHHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHCCCCEEEEEEHHHHHHHHH
SRIIDAFPAEQQDQIRTQLANVLLGVVSQRLFPRIDVPKGRVAAMEIMLGNPAVANLIRN
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEECCHHHHHHHCC
EKEFQLPTVIQTSRQHGMQSMDAAIEQLVRQGIINPNAVPTEEMR
CCCCCCHHHHHHHHHHCHHHHHHHHHHHHHHCCCCCCCCCHHCCC
>Mature Secondary Structure
MINQSIETILTNAVEKRASDIHLTAGSPPIIRVDGSLITMGEEKVLPHHLEGYLKEIMTD
CCCHHHHHHHHHHHHHHCCCEEEECCCCCEEEECCCEEECCCCCCCHHHHHHHHHHHHHH
DMRERLEHDRDLDFSFGVPRVSRFRVNAYYQRGTIAIAFRSISGEIPTLNELNLPTVLKR
HHHHHHHCCCCCCCCCCCCCHHHEEEEEEEECCEEEEEEEECCCCCCCCCCCCHHHHHHH
LIEKPHGLILVTGPTGSGKSTTLAAMIHEINRTQRKRIITLEDPIEYLHSHDQSIVDQRE
HHHCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHEEEEEHHHHHHHHCCCHHHHHHHH
IGSDVMKFVSGLRAALRQDPDVILLGEMRDLETISTAMTAAETGHLVMATLHTSSAMSTV
HHHHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHCCCCEEEEEEHHHHHHHHH
SRIIDAFPAEQQDQIRTQLANVLLGVVSQRLFPRIDVPKGRVAAMEIMLGNPAVANLIRN
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEECCHHHHHHHCC
EKEFQLPTVIQTSRQHGMQSMDAAIEQLVRQGIINPNAVPTEEMR
CCCCCCHHHHHHHHHHCHHHHHHHHHHHHHHCCCCCCCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1676385; 10984043 [H]