Click here to switch to the map view.

The map label for this gene is nfrA1 [H]

Identifier: 229918246

GI number: 229918246

Start: 2488859

End: 2489608

Strand: Direct

Name: nfrA1 [H]

Synonym: EAT1b_2527

Alternate gene names: 229918246

Gene position: 2488859-2489608 (Clockwise)

Preceding gene: 229918244

Following gene: 229918250

Centisome position: 82.96

GC content: 50.13

Gene sequence:

>750_bases
ATGAACGAAGTGATTGAGACCTTATTGAAACACCGTTCCGTCCGCGATTTTACGGACGAGAAATTGACAGACGAACAGAT
CCAAACCATCGTCGAGAGCGCACAACGGGCATCGACGTCGAGCTTTATCCAAGCCTACTCCATCATCGGGGTGACCGATG
AAGCGAAAAAGGAACGACTTGCCGAGATTGCGGGCAACCAGTCCTATGTCGTCGAGAACGGTCACTTCTTCGTTTTTTGT
GCGGATTTGCACCGCCATGAAGTGATTGGTGAACTGCAAGAAACAGAAGTGTCCTCAACACTTGAGACGGATGAGAAGTT
GCTTGTCGCGGTGATCGACGCTGCACTTGCCTCTCAAAATGCAGCAGTGGCGGCAGAGTCGATGGGGCTCGGAATTTGTT
ATATCGGTGGCATTCGGAACGACCTCTTTGCGGTGAAAGAAGTGCTCGCATTACCGGAACGGGTCATTCCGCTCTTCGGG
ATGGCCGTCGGTGTACCGGCCTCAGTCGAGGATCAAAAACCACGCTTACCGTTCCAACATGTCTACCATGAAAATACGTA
TGAAGCGAATCCAGAACGGATCAAGCAGTCCCTTTCCGATTACGATGCGTTAATCCACGATTACTACATGGTCCGTGGAT
CGAACCCGCGTAAAGATACGTGGACTGGCCAAATGGGGCGCATGTTGTCAAAATCGACGCGTCTTGACGTCAAAGACTTT
TTGAAAGCACAAGGTTATTTGAAGCAATAA

Upstream 100 bases:

>100_bases
CGAGATTTGAGCGTCTTCCCTCAAAATGTGCTCGATGGATTCCTCTCATTTGTGGATTCAGAGAGCTTATGCCAAGATTG
AAAAAAAGGGGGAAGAACGT

Downstream 100 bases:

>100_bases
GTTAAACCGCCGACAAATCACTGTCGGCGGTTTTGTCATGTTCAATTCAATTGGGCGTCGAGCCAATTCTTGTCGATGGC
ACCGAATTGTTTCGCGATGA

Product: nitroreductase

Products: NA

Alternate protein names: NADPH-dependent FMN reductase; NADPH-dependent nitroreductase; NADPH-dependent oxidoreductase [H]

Number of amino acids: Translated: 249; Mature: 249

Protein sequence:

>249_residues
MNEVIETLLKHRSVRDFTDEKLTDEQIQTIVESAQRASTSSFIQAYSIIGVTDEAKKERLAEIAGNQSYVVENGHFFVFC
ADLHRHEVIGELQETEVSSTLETDEKLLVAVIDAALASQNAAVAAESMGLGICYIGGIRNDLFAVKEVLALPERVIPLFG
MAVGVPASVEDQKPRLPFQHVYHENTYEANPERIKQSLSDYDALIHDYYMVRGSNPRKDTWTGQMGRMLSKSTRLDVKDF
LKAQGYLKQ

Sequences:

>Translated_249_residues
MNEVIETLLKHRSVRDFTDEKLTDEQIQTIVESAQRASTSSFIQAYSIIGVTDEAKKERLAEIAGNQSYVVENGHFFVFC
ADLHRHEVIGELQETEVSSTLETDEKLLVAVIDAALASQNAAVAAESMGLGICYIGGIRNDLFAVKEVLALPERVIPLFG
MAVGVPASVEDQKPRLPFQHVYHENTYEANPERIKQSLSDYDALIHDYYMVRGSNPRKDTWTGQMGRMLSKSTRLDVKDF
LKAQGYLKQ
>Mature_249_residues
MNEVIETLLKHRSVRDFTDEKLTDEQIQTIVESAQRASTSSFIQAYSIIGVTDEAKKERLAEIAGNQSYVVENGHFFVFC
ADLHRHEVIGELQETEVSSTLETDEKLLVAVIDAALASQNAAVAAESMGLGICYIGGIRNDLFAVKEVLALPERVIPLFG
MAVGVPASVEDQKPRLPFQHVYHENTYEANPERIKQSLSDYDALIHDYYMVRGSNPRKDTWTGQMGRMLSKSTRLDVKDF
LKAQGYLKQ

Specific function: Reduces FMNH(2) to FMN, with NADPH as reductant. It also reduces nitroaromatic compounds, quinones and azo dyes [H]

COG id: COG0778

COG function: function code C; Nitroreductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the flavin oxidoreductase frp family [H]

Homologues:

Organism=Escherichia coli, GI1787075, Length=246, Percent_Identity=43.4959349593496, Blast_Score=202, Evalue=1e-53,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016446
- InterPro:   IPR000415 [H]

Pfam domain/function: PF00881 Nitroreductase [H]

EC number: =1.5.1.29 [H]

Molecular weight: Translated: 27899; Mature: 27899

Theoretical pI: Translated: 4.93; Mature: 4.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNEVIETLLKHRSVRDFTDEKLTDEQIQTIVESAQRASTSSFIQAYSIIGVTDEAKKERL
CHHHHHHHHHHCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
AEIAGNQSYVVENGHFFVFCADLHRHEVIGELQETEVSSTLETDEKLLVAVIDAALASQN
HHHCCCCEEEEECCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
AAVAAESMGLGICYIGGIRNDLFAVKEVLALPERVIPLFGMAVGVPASVEDQKPRLPFQH
HHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHH
VYHENTYEANPERIKQSLSDYDALIHDYYMVRGSNPRKDTWTGQMGRMLSKSTRLDVKDF
HHCCCCCCCCHHHHHHHHHHHHHHHHHHHHEECCCCCCCCHHHHHHHHHHHHCCCCHHHH
LKAQGYLKQ
HHHCCCCCC
>Mature Secondary Structure
MNEVIETLLKHRSVRDFTDEKLTDEQIQTIVESAQRASTSSFIQAYSIIGVTDEAKKERL
CHHHHHHHHHHCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
AEIAGNQSYVVENGHFFVFCADLHRHEVIGELQETEVSSTLETDEKLLVAVIDAALASQN
HHHCCCCEEEEECCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
AAVAAESMGLGICYIGGIRNDLFAVKEVLALPERVIPLFGMAVGVPASVEDQKPRLPFQH
HHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHH
VYHENTYEANPERIKQSLSDYDALIHDYYMVRGSNPRKDTWTGQMGRMLSKSTRLDVKDF
HHCCCCCCCCHHHHHHHHHHHHHHHHHHHHEECCCCCCCCHHHHHHHHHHHHCCCCHHHH
LKAQGYLKQ
HHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7934828; 9384377; 9836433 [H]