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The map label for this gene is pflB [H]

Identifier: 229918071

GI number: 229918071

Start: 2303893

End: 2306139

Strand: Direct

Name: pflB [H]

Synonym: EAT1b_2350

Alternate gene names: 229918071

Gene position: 2303893-2306139 (Clockwise)

Preceding gene: 229918067

Following gene: 229918072

Centisome position: 76.8

GC content: 48.55

Gene sequence:

>2247_bases
ATGGCAACCGAAATCAAATCCGCATGGTCAGGTTTTATCCCTGGTCAGTGGACGAATGAAGTGAATGTCAGTGAATTTAT
CCGTTTGAACCGTCATGAGTACACAGGAGACGATACGTTTTTAGCAGGTCCGACTGAAGCGACAAACGTTCTTTGGAATC
GTGTGATGGAACTAACAAAAGAAGAGCGTGAACGTGGAGGCGTCTGGGACGTCGATCAACACACACCATCGACAATTGTG
TCGCATGGACCAGGTTACTTAAACAAAGAGTTAGAGAAAGTCGTTGGCGTTCAGACGGATGAGCCGTTCAAACGTTCGAT
TCACCCGAATGGCGGCATCCGTATGGTCGATGCGGCACTCGAATCATATGGTTTCGAGCCAGACGAAGAAATCACAAAAA
TTTACTCAGAGATTCGTAAAACACATAACCAAGGTGTCTTCGATGCCTATACACCAGAAATGCGGGCCGCTCGTAAATCA
GGAATCATTACAGGGCTTCCCGATGCATACGGTCGCGGACGCATCATCGGTGACTACCGTCGTGTCGCCCTTTATGGAAT
CGACTTCTTGATGGCAGAACGCAAAAAAGACTTGGCAAAACGTGGCGGTTTCTTATCTGAATCGGACATCCGTGACCGCG
AAGAACTATCGGAACAACTTCGTGCCCTCCAAGAACTTAAACAACTCGGAGCCGCTTATGGATTTGACCTCGGTCGTCCT
GCGGAAAATACGCAAGAAGCATATCAATGGGTATACTTGGCGTATCTTGCTGCCATCAAAGAACAAAACGGTGCCGCAAT
GTCACTCGGTCGCGTCTCGACATTCCTTGATGTGTACGCCGAACGTGACCTTGAAGCTGGTCGCTTAACAGAATCGGACG
TCCAAGAAATCGTTGACCACTTCATCATGAAACTTCGAATCGTCAAATTCTTACGGACACCGGACTATAACGAGTTATTC
TCTGGTGACCCGACTTGGGTGACGGAATCAATCGGTGGGATGAGTCAAGATGGTCGCTCGAACGTCACGAAGAGCTCATT
CCGGTTCTTACATTCACTCACGAACCTCGGACCAGCTCCAGAACCAAACTTGACGGTCCTTTGGTCGACGAAACTTCCAG
AAGGCTTCAAACAATACTGTGCGAAAATGTCGATTGAAACGTCGGCTATTCAATATGAGAACGATGACTTGATGATGCCA
CAATTCGGTGATGACTACGGTATCGCATGTTGTGTGTCACCGATGAAGATCGGGAAACAAATGCAGTTCTTCGGCGCGCG
TGCCAACATGGCGAAAGCCCTTCTTTATAGCATGAACGGCGGACGTGATGAGAAGAGCGGCGCTCAAATCGCTCCAGTTT
GGGAAATGAACACAGAAGACGTGCTTACGTACGAAAAAGTGTATGCCGACTTTGACCGTACGCTCGACTGGTTGGCGGAG
CTATACGTGAATACACTTAACGTCATTCACTTCATGCACGATAAATATGCGTACGAACGAATCGAAATGGCGCTTCACGA
TCCTGAAATCTTACGGACGATGGCTTGTGGGATCGCGGGTCTCTCGGTAACGGCAGATAGTCTTTCAGCAATCAAATATG
CAAACGTCAAACCCGTCCGGAATGAAGAAGGAATCATCGTCGACTTCGAAACAGAAGGAGACTTCCCGAAATTCGGAAAC
AATGATGACCGTGTCGATGCAATCGCCGTCGAACTCGTCGAATCATTCATGGAGAAAGTGCGGAAGCATAAAACATACCG
TGACGCGCTTCACACACAATCGGTTCTCACGATCACATCAAACGTCGTTTACGGTAAGAAAACAGGAAATACGCCAGACG
GTCGTCGTGCGGGTGAACCATTTGCTCCAGGTGCAAACCCAATGCATGGACGGGACACAAAAGGTGCGGCTGCATCGCTC
TCATCTGTTGCGAAACTTCCATTCGAACACGCACAAGACGGAATCTCTTACACGTTCTCAATCGTACCGAAAGCACTCGG
AAAAGAAGAAATCGCACGCGAACTCAACTTGGCGGCGCTTCTCGATGGATACATGAGTGGAGAGCACAAAGGACATCACT
TGAACGTCAACGTCTTTAACCGCGAGACGCTTCTCGACGCAATGGAACATCCAGAAGAATATCCGCAACTCACAATCCGC
GTATCAGGATATGCGGTCAACTTCATCAAGTTGACACGTGAGCAACAGATCGACGTCATCAACCGTACGTTCCACGGTTC
GTTGTAA

Upstream 100 bases:

>100_bases
ACAAGTTTCACAAGAATGTAAAACATATTGTTCACGTTTTCACGTACACTCACAGTATAGATAACACGCAGTTACTTTCG
AGAATGAGGAGTGAATCAAC

Downstream 100 bases:

>100_bases
GTAAAGGCTTAGCGGCCTTCCACGGAGGGCCGCTCATTCGTTAGGAGGTCATTTCGATGACAATGGGATATGTACATTCG
GTCGAATCATTCGGAACAGT

Product: formate acetyltransferase

Products: NA

Alternate protein names: Pyruvate formate-lyase [H]

Number of amino acids: Translated: 748; Mature: 747

Protein sequence:

>748_residues
MATEIKSAWSGFIPGQWTNEVNVSEFIRLNRHEYTGDDTFLAGPTEATNVLWNRVMELTKEERERGGVWDVDQHTPSTIV
SHGPGYLNKELEKVVGVQTDEPFKRSIHPNGGIRMVDAALESYGFEPDEEITKIYSEIRKTHNQGVFDAYTPEMRAARKS
GIITGLPDAYGRGRIIGDYRRVALYGIDFLMAERKKDLAKRGGFLSESDIRDREELSEQLRALQELKQLGAAYGFDLGRP
AENTQEAYQWVYLAYLAAIKEQNGAAMSLGRVSTFLDVYAERDLEAGRLTESDVQEIVDHFIMKLRIVKFLRTPDYNELF
SGDPTWVTESIGGMSQDGRSNVTKSSFRFLHSLTNLGPAPEPNLTVLWSTKLPEGFKQYCAKMSIETSAIQYENDDLMMP
QFGDDYGIACCVSPMKIGKQMQFFGARANMAKALLYSMNGGRDEKSGAQIAPVWEMNTEDVLTYEKVYADFDRTLDWLAE
LYVNTLNVIHFMHDKYAYERIEMALHDPEILRTMACGIAGLSVTADSLSAIKYANVKPVRNEEGIIVDFETEGDFPKFGN
NDDRVDAIAVELVESFMEKVRKHKTYRDALHTQSVLTITSNVVYGKKTGNTPDGRRAGEPFAPGANPMHGRDTKGAAASL
SSVAKLPFEHAQDGISYTFSIVPKALGKEEIARELNLAALLDGYMSGEHKGHHLNVNVFNRETLLDAMEHPEEYPQLTIR
VSGYAVNFIKLTREQQIDVINRTFHGSL

Sequences:

>Translated_748_residues
MATEIKSAWSGFIPGQWTNEVNVSEFIRLNRHEYTGDDTFLAGPTEATNVLWNRVMELTKEERERGGVWDVDQHTPSTIV
SHGPGYLNKELEKVVGVQTDEPFKRSIHPNGGIRMVDAALESYGFEPDEEITKIYSEIRKTHNQGVFDAYTPEMRAARKS
GIITGLPDAYGRGRIIGDYRRVALYGIDFLMAERKKDLAKRGGFLSESDIRDREELSEQLRALQELKQLGAAYGFDLGRP
AENTQEAYQWVYLAYLAAIKEQNGAAMSLGRVSTFLDVYAERDLEAGRLTESDVQEIVDHFIMKLRIVKFLRTPDYNELF
SGDPTWVTESIGGMSQDGRSNVTKSSFRFLHSLTNLGPAPEPNLTVLWSTKLPEGFKQYCAKMSIETSAIQYENDDLMMP
QFGDDYGIACCVSPMKIGKQMQFFGARANMAKALLYSMNGGRDEKSGAQIAPVWEMNTEDVLTYEKVYADFDRTLDWLAE
LYVNTLNVIHFMHDKYAYERIEMALHDPEILRTMACGIAGLSVTADSLSAIKYANVKPVRNEEGIIVDFETEGDFPKFGN
NDDRVDAIAVELVESFMEKVRKHKTYRDALHTQSVLTITSNVVYGKKTGNTPDGRRAGEPFAPGANPMHGRDTKGAAASL
SSVAKLPFEHAQDGISYTFSIVPKALGKEEIARELNLAALLDGYMSGEHKGHHLNVNVFNRETLLDAMEHPEEYPQLTIR
VSGYAVNFIKLTREQQIDVINRTFHGSL
>Mature_747_residues
ATEIKSAWSGFIPGQWTNEVNVSEFIRLNRHEYTGDDTFLAGPTEATNVLWNRVMELTKEERERGGVWDVDQHTPSTIVS
HGPGYLNKELEKVVGVQTDEPFKRSIHPNGGIRMVDAALESYGFEPDEEITKIYSEIRKTHNQGVFDAYTPEMRAARKSG
IITGLPDAYGRGRIIGDYRRVALYGIDFLMAERKKDLAKRGGFLSESDIRDREELSEQLRALQELKQLGAAYGFDLGRPA
ENTQEAYQWVYLAYLAAIKEQNGAAMSLGRVSTFLDVYAERDLEAGRLTESDVQEIVDHFIMKLRIVKFLRTPDYNELFS
GDPTWVTESIGGMSQDGRSNVTKSSFRFLHSLTNLGPAPEPNLTVLWSTKLPEGFKQYCAKMSIETSAIQYENDDLMMPQ
FGDDYGIACCVSPMKIGKQMQFFGARANMAKALLYSMNGGRDEKSGAQIAPVWEMNTEDVLTYEKVYADFDRTLDWLAEL
YVNTLNVIHFMHDKYAYERIEMALHDPEILRTMACGIAGLSVTADSLSAIKYANVKPVRNEEGIIVDFETEGDFPKFGNN
DDRVDAIAVELVESFMEKVRKHKTYRDALHTQSVLTITSNVVYGKKTGNTPDGRRAGEPFAPGANPMHGRDTKGAAASLS
SVAKLPFEHAQDGISYTFSIVPKALGKEEIARELNLAALLDGYMSGEHKGHHLNVNVFNRETLLDAMEHPEEYPQLTIRV
SGYAVNFIKLTREQQIDVINRTFHGSL

Specific function: Glucose metabolism (nonoxidative conversion). [C]

COG id: COG1882

COG function: function code C; Pyruvate-formate lyase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 pyruvate formate lyase domain [H]

Homologues:

Organism=Escherichia coli, GI1787131, Length=754, Percent_Identity=63.395225464191, Blast_Score=1007, Evalue=0.0,
Organism=Escherichia coli, GI48994926, Length=750, Percent_Identity=61.6, Blast_Score=971, Evalue=0.0,
Organism=Escherichia coli, GI1787044, Length=618, Percent_Identity=26.0517799352751, Blast_Score=175, Evalue=1e-44,
Organism=Escherichia coli, GI1790388, Length=683, Percent_Identity=25.7686676427526, Blast_Score=162, Evalue=7e-41,
Organism=Escherichia coli, GI1788933, Length=58, Percent_Identity=74.1379310344828, Blast_Score=88, Evalue=2e-18,

Paralogues:

None

Copy number: 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005949
- InterPro:   IPR001150
- InterPro:   IPR019777
- InterPro:   IPR004184 [H]

Pfam domain/function: PF01228 Gly_radical; PF02901 PFL [H]

EC number: =2.3.1.54 [H]

Molecular weight: Translated: 84024; Mature: 83893

Theoretical pI: Translated: 5.14; Mature: 5.14

Prosite motif: PS00850 GLY_RADICAL_1 ; PS51149 GLY_RADICAL_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATEIKSAWSGFIPGQWTNEVNVSEFIRLNRHEYTGDDTFLAGPTEATNVLWNRVMELTK
CCCCHHHHHCCCCCCCCCCCCCHHHHHHHCCCCCCCCCCEECCCCHHHHHHHHHHHHHHH
EERERGGVWDVDQHTPSTIVSHGPGYLNKELEKVVGVQTDEPFKRSIHPNGGIRMVDAAL
HHHHCCCCCCCCCCCCHHHHHCCCCHHHHHHHHHHCCCCCCCHHHCCCCCCCEEHHHHHH
ESYGFEPDEEITKIYSEIRKTHNQGVFDAYTPEMRAARKSGIITGLPDAYGRGRIIGDYR
HHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCEECCCCCCCCCCEEHHHH
RVALYGIDFLMAERKKDLAKRGGFLSESDIRDREELSEQLRALQELKQLGAAYGFDLGRP
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCC
AENTQEAYQWVYLAYLAAIKEQNGAAMSLGRVSTFLDVYAERDLEAGRLTESDVQEIVDH
CCHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHH
FIMKLRIVKFLRTPDYNELFSGDPTWVTESIGGMSQDGRSNVTKSSFRFLHSLTNLGPAP
HHHHHHHHHHHCCCCHHHHHCCCCCCHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCC
EPNLTVLWSTKLPEGFKQYCAKMSIETSAIQYENDDLMMPQFGDDYGIACCVSPMKIGKQ
CCCEEEEECCCCCHHHHHHHHHCCCCHHEEEECCCCEECCCCCCCCCEEEECCHHHHCCH
MQFFGARANMAKALLYSMNGGRDEKSGAQIAPVWEMNTEDVLTYEKVYADFDRTLDWLAE
HHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHH
LYVNTLNVIHFMHDKYAYERIEMALHDPEILRTMACGIAGLSVTADSLSAIKYANVKPVR
HHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCEEEHHHHHHHHCCCCCCCC
NEEGIIVDFETEGDFPKFGNNDDRVDAIAVELVESFMEKVRKHKTYRDALHTQSVLTITS
CCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEC
NVVYGKKTGNTPDGRRAGEPFAPGANPMHGRDTKGAAASLSSVAKLPFEHAQDGISYTFS
CEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCHHHHHCCCEEEHH
IVPKALGKEEIARELNLAALLDGYMSGEHKGHHLNVNVFNRETLLDAMEHPEEYPQLTIR
HHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECHHHHHHHHHCCCCCCEEEEE
VSGYAVNFIKLTREQQIDVINRTFHGSL
EECEEEEEEEHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
ATEIKSAWSGFIPGQWTNEVNVSEFIRLNRHEYTGDDTFLAGPTEATNVLWNRVMELTK
CCCHHHHHCCCCCCCCCCCCCHHHHHHHCCCCCCCCCCEECCCCHHHHHHHHHHHHHHH
EERERGGVWDVDQHTPSTIVSHGPGYLNKELEKVVGVQTDEPFKRSIHPNGGIRMVDAAL
HHHHCCCCCCCCCCCCHHHHHCCCCHHHHHHHHHHCCCCCCCHHHCCCCCCCEEHHHHHH
ESYGFEPDEEITKIYSEIRKTHNQGVFDAYTPEMRAARKSGIITGLPDAYGRGRIIGDYR
HHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCEECCCCCCCCCCEEHHHH
RVALYGIDFLMAERKKDLAKRGGFLSESDIRDREELSEQLRALQELKQLGAAYGFDLGRP
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCC
AENTQEAYQWVYLAYLAAIKEQNGAAMSLGRVSTFLDVYAERDLEAGRLTESDVQEIVDH
CCHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHH
FIMKLRIVKFLRTPDYNELFSGDPTWVTESIGGMSQDGRSNVTKSSFRFLHSLTNLGPAP
HHHHHHHHHHHCCCCHHHHHCCCCCCHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCC
EPNLTVLWSTKLPEGFKQYCAKMSIETSAIQYENDDLMMPQFGDDYGIACCVSPMKIGKQ
CCCEEEEECCCCCHHHHHHHHHCCCCHHEEEECCCCEECCCCCCCCCEEEECCHHHHCCH
MQFFGARANMAKALLYSMNGGRDEKSGAQIAPVWEMNTEDVLTYEKVYADFDRTLDWLAE
HHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHH
LYVNTLNVIHFMHDKYAYERIEMALHDPEILRTMACGIAGLSVTADSLSAIKYANVKPVR
HHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCEEEHHHHHHHHCCCCCCCC
NEEGIIVDFETEGDFPKFGNNDDRVDAIAVELVESFMEKVRKHKTYRDALHTQSVLTITS
CCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEC
NVVYGKKTGNTPDGRRAGEPFAPGANPMHGRDTKGAAASLSSVAKLPFEHAQDGISYTFS
CEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCHHHHHCCCEEEHH
IVPKALGKEEIARELNLAALLDGYMSGEHKGHHLNVNVFNRETLLDAMEHPEEYPQLTIR
HHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECHHHHHHHHHCCCCCCEEEEE
VSGYAVNFIKLTREQQIDVINRTFHGSL
EECEEEEEEEHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA