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The map label for this gene is pflA [H]

Identifier: 229918072

GI number: 229918072

Start: 2306196

End: 2306912

Strand: Direct

Name: pflA [H]

Synonym: EAT1b_2351

Alternate gene names: 229918072

Gene position: 2306196-2306912 (Clockwise)

Preceding gene: 229918071

Following gene: 229918074

Centisome position: 76.88

GC content: 51.19

Gene sequence:

>717_bases
ATGACAATGGGATATGTACATTCGGTCGAATCATTCGGAACAGTTGACGGCCCAGGGATTCGCTTCATCGTCTTTTTACA
AGGTTGTGCCTTGCGTTGTTTATACTGCCATAATGCGGACACGTGGGATTTCAAAAAGAATAACCACCGCTCGGCTGAAG
ACGTCATTCAAGAGGCGCTCAGTTACCGTCCGTTCATGGAAGCATCGAAGGGGGGCATCACTATTTCTGGTGGTGACCCA
CTCGCGCAACCTGAATTTTTAGAGGCGCTACTGCGTGAAGCGAAAAAGCATGGGCTTCATACGACACTTGATACGTCGGG
TGCGCTCCGACCTCCTAATTTGGATGCGATTTTAGATCACACCGACCTTGTCTTGCTCGATATCAAGCATATCGACGACG
ACATGTGTAAGAAGTTGACGGGACGTAGCAATGCCAACACGCTCGCGCTTGCCGAACACCTTTCCGAGCGCGGAACGAAA
ATGTGGATTCGCCACGTCCTTGTACCTGGATGGACGCTTGAGGAAGGTGCGCTTCGTCGCACCGCTGCGTTCATTCAAAA
GTTAGACCATGTCGAGAAAGTCGAGATTTTGCCGTACCATGAGATGGGCGTCTACAAATGGGAGGCGCTCGGGCTCGATT
ATCCGCTTAAAGGCACGAAGCCCCCGACATCCGATGAAGTCGAATGGGCGGAAGGAATCTTGCAGGGAACTGTATAA

Upstream 100 bases:

>100_bases
AACAGATCGACGTCATCAACCGTACGTTCCACGGTTCGTTGTAAGTAAAGGCTTAGCGGCCTTCCACGGAGGGCCGCTCA
TTCGTTAGGAGGTCATTTCG

Downstream 100 bases:

>100_bases
AACAAGCGCAGACCGATGTGGTCTGCGCTTGTTTGACGTTATACTCGTTTTTTCTTCTTTTCTAAGACGCGGGCATGTTC
TGCCTGTTTCTTCTCCATAT

Product: pyruvate formate-lyase activating enzyme

Products: NA

Alternate protein names: Formate-C-acetyltransferase-activating enzyme 1; PFL-activating enzyme 1 [H]

Number of amino acids: Translated: 238; Mature: 237

Protein sequence:

>238_residues
MTMGYVHSVESFGTVDGPGIRFIVFLQGCALRCLYCHNADTWDFKKNNHRSAEDVIQEALSYRPFMEASKGGITISGGDP
LAQPEFLEALLREAKKHGLHTTLDTSGALRPPNLDAILDHTDLVLLDIKHIDDDMCKKLTGRSNANTLALAEHLSERGTK
MWIRHVLVPGWTLEEGALRRTAAFIQKLDHVEKVEILPYHEMGVYKWEALGLDYPLKGTKPPTSDEVEWAEGILQGTV

Sequences:

>Translated_238_residues
MTMGYVHSVESFGTVDGPGIRFIVFLQGCALRCLYCHNADTWDFKKNNHRSAEDVIQEALSYRPFMEASKGGITISGGDP
LAQPEFLEALLREAKKHGLHTTLDTSGALRPPNLDAILDHTDLVLLDIKHIDDDMCKKLTGRSNANTLALAEHLSERGTK
MWIRHVLVPGWTLEEGALRRTAAFIQKLDHVEKVEILPYHEMGVYKWEALGLDYPLKGTKPPTSDEVEWAEGILQGTV
>Mature_237_residues
TMGYVHSVESFGTVDGPGIRFIVFLQGCALRCLYCHNADTWDFKKNNHRSAEDVIQEALSYRPFMEASKGGITISGGDPL
AQPEFLEALLREAKKHGLHTTLDTSGALRPPNLDAILDHTDLVLLDIKHIDDDMCKKLTGRSNANTLALAEHLSERGTKM
WIRHVLVPGWTLEEGALRRTAAFIQKLDHVEKVEILPYHEMGVYKWEALGLDYPLKGTKPPTSDEVEWAEGILQGTV

Specific function: Activation of pyruvate formate-lyase 1 under anaerobic conditions by generation of an organic free radical, using S- adenosylmethionine and reduced flavodoxin as cosubstrates to produce 5'-deoxy-adenosine [H]

COG id: COG1180

COG function: function code O; Pyruvate-formate lyase-activating enzyme

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the organic radical-activating enzymes family [H]

Homologues:

Organism=Escherichia coli, GI1787130, Length=236, Percent_Identity=48.3050847457627, Blast_Score=251, Evalue=3e-68,
Organism=Escherichia coli, GI1790389, Length=255, Percent_Identity=23.5294117647059, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI226510931, Length=165, Percent_Identity=33.3333333333333, Blast_Score=82, Evalue=4e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR012838
- InterPro:   IPR001989
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: =1.97.1.4 [H]

Molecular weight: Translated: 26576; Mature: 26444

Theoretical pI: Translated: 5.94; Mature: 5.94

Prosite motif: PS01087 RADICAL_ACTIVATING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTMGYVHSVESFGTVDGPGIRFIVFLQGCALRCLYCHNADTWDFKKNNHRSAEDVIQEAL
CCCCCHHHHHHCCCCCCCCCEEEEEEHHHHEEEEEECCCCCCCCCCCCCCCHHHHHHHHH
SYRPFMEASKGGITISGGDPLAQPEFLEALLREAKKHGLHTTLDTSGALRPPNLDAILDH
HCCCHHHCCCCCEEEECCCCCCCHHHHHHHHHHHHHCCCCEEECCCCCCCCCCCHHHCCC
TDLVLLDIKHIDDDMCKKLTGRSNANTLALAEHLSERGTKMWIRHVLVPGWTLEEGALRR
CCEEEEEHHHCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHH
TAAFIQKLDHVEKVEILPYHEMGVYKWEALGLDYPLKGTKPPTSDEVEWAEGILQGTV
HHHHHHHHHCHHHEEECCCHHCCCEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHCCC
>Mature Secondary Structure 
TMGYVHSVESFGTVDGPGIRFIVFLQGCALRCLYCHNADTWDFKKNNHRSAEDVIQEAL
CCCCHHHHHHCCCCCCCCCEEEEEEHHHHEEEEEECCCCCCCCCCCCCCCHHHHHHHHH
SYRPFMEASKGGITISGGDPLAQPEFLEALLREAKKHGLHTTLDTSGALRPPNLDAILDH
HCCCHHHCCCCCEEEECCCCCCCHHHHHHHHHHHHHCCCCEEECCCCCCCCCCCHHHCCC
TDLVLLDIKHIDDDMCKKLTGRSNANTLALAEHLSERGTKMWIRHVLVPGWTLEEGALRR
CCEEEEEHHHCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHH
TAAFIQKLDHVEKVEILPYHEMGVYKWEALGLDYPLKGTKPPTSDEVEWAEGILQGTV
HHHHHHHHHCHHHEEECCCHHCCCEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]