Click here to switch to the map view.

The map label for this gene is ligA [H]

Identifier: 229917963

GI number: 229917963

Start: 2192758

End: 2194752

Strand: Direct

Name: ligA [H]

Synonym: EAT1b_2242

Alternate gene names: 229917963

Gene position: 2192758-2194752 (Clockwise)

Preceding gene: 229917962

Following gene: 229917964

Centisome position: 73.09

GC content: 52.08

Gene sequence:

>1995_bases
ATGGAGATGGCAGAACGCGTAGAAGAGCTACGTCAAACGTTAAATCAATATGGTTACGAGTATTACGTACTCGACCGTCC
GAGCGTGCCGGATTCGGAGTATGACCGATTGATGAACGAATTGATTCAAATCGAGACTGATTATCCTGAACTTCGAACAC
CGGATTCTCCGACGCAACGCGTCGGAGGGGCTCCGCTCGACGCATTTGTCAAAGTGACACATGACACGCCGATGCTCTCG
CTCGGCAACGTCTTCTCGAAAGAGGAACTCGTCGAGTGGGTGGAGCGAATCGAGAAAGATCTCGGCTACACGCCGACATT
CGTGGCAGAACTCAAATTTGATGGTCTCGCTGTCTCGTTAAAATATGAGAATGGCCGTCTCGTCCGTGGTGCGACGCGCG
GGGACGGGACGACGGGTGAGGACATCACGCAAAACTTGAAGACGATTCGTTCCATCCCTCTCCGATTAAATGAGGACGTG
ACGTTTGAAGTGCGAGGTGAGGCGTACATGCCGAAACGTTCGTTCGAACAATTGAATGACGAACGAGAACGTATCGGCGA
ACCACTTTTCGCAAATCCGAGAAACGCTGCTGCCGGTTCACTTCGACAACTCGACTCAAAAATTGCGGCGTCAAGAAATT
TGGCGTTCTTCGCATACGGTTTGGTGTCGCAAGACGATCTCGTCGCGTCACATGACGAATCACTCGATTATTTGAAACGA
CTCGGTGTCGCAGTGAGCGGAGATTATACAACATGCCAGACGGCGGATGAACTGTGGGATTACATTGAGACATACGTGAC
AAAACGGAGTGACCTCCCATATGAAATTGATGGGATTGTCATCAAAGTGGCCGGGTATGAAGAACAGGAACAACTTGGTT
TCACCGCTAAAAGTCCGCGCTGGGCCGTCGCGTACAAGTTCCCGGCAGAAGAAGTCGTGACGACGATCGAGGACGTCGAT
TTCAGTGTCGGTCGTACGGGGAAGGTCACACCACGGGCACGATTTGCCCCGGTTTCTGTGGCGGGGTCGACCGTGACGTA
TGCGACGCTTCACAATGAAGATTTTATAAAGGAAAAAGACCTTCATATCGGTGACCGAGTCGTCATCAAAAAAGCAGGAG
ACGTCATTCCGGCCGTCGTATCGGCACTGGCGAGTGAACGGAGCGGGGAAGAACGGGTGATTGAATTCCCAACGCATTGT
CCGGCGTGTGGGTCGGAGTTGATTCGACTCGAAGGAGAGGCGGATCATCGTTGCGTCAATCCGGAATGTCCGGCTCAACT
GCTTGAAGGACTCATTCATTTCGTGTCGAGACAAGCGATGAACATCGACGGGCTCGGCGAGAAAGTCATCACACAGCTTC
ACGAAGCGGGGCTCGTTCACAATGTGGCCGACCTGTATCGCTTGGACCGAGATGATTTACTTGGACTCGAGCGCATGGGC
GAGACGTCAGTGACGAACTTGCTCTCGGCAATCGAACAGTCGAAGCAAAATTCATTGGAACGGGTGCTCTTCGCCCTCGG
GATTCGACTCGTCGGACAAAAGGCCGCTTCTTTAATTGCAGAACGGTTCGAGACGATGGATGCGATCATCGCTGCGACAG
TTGAAGAGTTGACATCCATCGATGGCATCGGGACGAAGATGGCCGAATCGATTGTCCTCTATTTCGAAAAACCAGAAGCA
CGTGAACTCGTCAAAGAATTGGCGGAATCAGGTGTGAACTTATCATTCAAAGGAATGAAGCGTCCAACGACTGAAGGGGC
ACCGCTCTCTGGGAAGACAGTCGTATTGACGGGGAAATTGCATGAGATGACACGTGGCGAGGCTGGGAAGCAGCTTGAAC
TCCTCGGCGCGAGTGTGACAGGGAGCGTGAGTAAAAACACAGACTTGCTCGTCGCCGGTGAAAAGGCAGGTTCGAAGCTG
GCGAAAGCCGAATCTCTCGGAATCGAAGTATGGGATGAAGCCAAGCTGCTCGACTTTTTAAAGGAACATGCATAA

Upstream 100 bases:

>100_bases
TGAAGGCGACCAACTCGAAGTCGACATCGCATTCCCGGAAGTCGGGATCAAGCGCCTATTGGCTAAATTTGCCCCTATCG
AAAAAGCGTGAGGTGAGAAT

Downstream 100 bases:

>100_bases
ACGTTCGCTTCGGCGAACGTTCAGCTTGTTGACAAATAACATGTTCGAACTCGTCCGTCGGGCGAGCAACGCAAACGGTC
GTCATCTGGCGACCGTTTGT

Product: DNA ligase, NAD-dependent

Products: NA

Alternate protein names: Polydeoxyribonucleotide synthase [NAD+] [H]

Number of amino acids: Translated: 664; Mature: 664

Protein sequence:

>664_residues
MEMAERVEELRQTLNQYGYEYYVLDRPSVPDSEYDRLMNELIQIETDYPELRTPDSPTQRVGGAPLDAFVKVTHDTPMLS
LGNVFSKEELVEWVERIEKDLGYTPTFVAELKFDGLAVSLKYENGRLVRGATRGDGTTGEDITQNLKTIRSIPLRLNEDV
TFEVRGEAYMPKRSFEQLNDERERIGEPLFANPRNAAAGSLRQLDSKIAASRNLAFFAYGLVSQDDLVASHDESLDYLKR
LGVAVSGDYTTCQTADELWDYIETYVTKRSDLPYEIDGIVIKVAGYEEQEQLGFTAKSPRWAVAYKFPAEEVVTTIEDVD
FSVGRTGKVTPRARFAPVSVAGSTVTYATLHNEDFIKEKDLHIGDRVVIKKAGDVIPAVVSALASERSGEERVIEFPTHC
PACGSELIRLEGEADHRCVNPECPAQLLEGLIHFVSRQAMNIDGLGEKVITQLHEAGLVHNVADLYRLDRDDLLGLERMG
ETSVTNLLSAIEQSKQNSLERVLFALGIRLVGQKAASLIAERFETMDAIIAATVEELTSIDGIGTKMAESIVLYFEKPEA
RELVKELAESGVNLSFKGMKRPTTEGAPLSGKTVVLTGKLHEMTRGEAGKQLELLGASVTGSVSKNTDLLVAGEKAGSKL
AKAESLGIEVWDEAKLLDFLKEHA

Sequences:

>Translated_664_residues
MEMAERVEELRQTLNQYGYEYYVLDRPSVPDSEYDRLMNELIQIETDYPELRTPDSPTQRVGGAPLDAFVKVTHDTPMLS
LGNVFSKEELVEWVERIEKDLGYTPTFVAELKFDGLAVSLKYENGRLVRGATRGDGTTGEDITQNLKTIRSIPLRLNEDV
TFEVRGEAYMPKRSFEQLNDERERIGEPLFANPRNAAAGSLRQLDSKIAASRNLAFFAYGLVSQDDLVASHDESLDYLKR
LGVAVSGDYTTCQTADELWDYIETYVTKRSDLPYEIDGIVIKVAGYEEQEQLGFTAKSPRWAVAYKFPAEEVVTTIEDVD
FSVGRTGKVTPRARFAPVSVAGSTVTYATLHNEDFIKEKDLHIGDRVVIKKAGDVIPAVVSALASERSGEERVIEFPTHC
PACGSELIRLEGEADHRCVNPECPAQLLEGLIHFVSRQAMNIDGLGEKVITQLHEAGLVHNVADLYRLDRDDLLGLERMG
ETSVTNLLSAIEQSKQNSLERVLFALGIRLVGQKAASLIAERFETMDAIIAATVEELTSIDGIGTKMAESIVLYFEKPEA
RELVKELAESGVNLSFKGMKRPTTEGAPLSGKTVVLTGKLHEMTRGEAGKQLELLGASVTGSVSKNTDLLVAGEKAGSKL
AKAESLGIEVWDEAKLLDFLKEHA
>Mature_664_residues
MEMAERVEELRQTLNQYGYEYYVLDRPSVPDSEYDRLMNELIQIETDYPELRTPDSPTQRVGGAPLDAFVKVTHDTPMLS
LGNVFSKEELVEWVERIEKDLGYTPTFVAELKFDGLAVSLKYENGRLVRGATRGDGTTGEDITQNLKTIRSIPLRLNEDV
TFEVRGEAYMPKRSFEQLNDERERIGEPLFANPRNAAAGSLRQLDSKIAASRNLAFFAYGLVSQDDLVASHDESLDYLKR
LGVAVSGDYTTCQTADELWDYIETYVTKRSDLPYEIDGIVIKVAGYEEQEQLGFTAKSPRWAVAYKFPAEEVVTTIEDVD
FSVGRTGKVTPRARFAPVSVAGSTVTYATLHNEDFIKEKDLHIGDRVVIKKAGDVIPAVVSALASERSGEERVIEFPTHC
PACGSELIRLEGEADHRCVNPECPAQLLEGLIHFVSRQAMNIDGLGEKVITQLHEAGLVHNVADLYRLDRDDLLGLERMG
ETSVTNLLSAIEQSKQNSLERVLFALGIRLVGQKAASLIAERFETMDAIIAATVEELTSIDGIGTKMAESIVLYFEKPEA
RELVKELAESGVNLSFKGMKRPTTEGAPLSGKTVVLTGKLHEMTRGEAGKQLELLGASVTGSVSKNTDLLVAGEKAGSKL
AKAESLGIEVWDEAKLLDFLKEHA

Specific function: DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of dam

COG id: COG0272

COG function: function code L; NAD-dependent DNA ligase (contains BRCT domain type II)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 BRCT domain [H]

Homologues:

Organism=Escherichia coli, GI1788750, Length=664, Percent_Identity=50.6024096385542, Blast_Score=646, Evalue=0.0,
Organism=Escherichia coli, GI87082305, Length=567, Percent_Identity=23.8095238095238, Blast_Score=134, Evalue=2e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001357
- InterPro:   IPR018239
- InterPro:   IPR004150
- InterPro:   IPR001679
- InterPro:   IPR013839
- InterPro:   IPR013840
- InterPro:   IPR003583
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR010994
- InterPro:   IPR004149 [H]

Pfam domain/function: PF00533 BRCT; PF01653 DNA_ligase_aden; PF03120 DNA_ligase_OB; PF03119 DNA_ligase_ZBD [H]

EC number: =6.5.1.2 [H]

Molecular weight: Translated: 73391; Mature: 73391

Theoretical pI: Translated: 4.59; Mature: 4.59

Prosite motif: PS50172 BRCT ; PS01055 DNA_LIGASE_N1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEMAERVEELRQTLNQYGYEYYVLDRPSVPDSEYDRLMNELIQIETDYPELRTPDSPTQR
CCHHHHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHHEECCCCCCCCCCCCHHHH
VGGAPLDAFVKVTHDTPMLSLGNVFSKEELVEWVERIEKDLGYTPTFVAELKFDGLAVSL
CCCCCHHHEEEEECCCCCHHHCCCCCHHHHHHHHHHHHHHCCCCCEEEEEEEECCEEEEE
KYENGRLVRGATRGDGTTGEDITQNLKTIRSIPLRLNEDVTFEVRGEAYMPKRSFEQLND
EECCCEEEEECCCCCCCCHHHHHHHHHHHHHCCCEECCCEEEEECCCCCCCHHHHHHHHH
ERERIGEPLFANPRNAAAGSLRQLDSKIAASRNLAFFAYGLVSQDDLVASHDESLDYLKR
HHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCHHHCCCHHHHHHHH
LGVAVSGDYTTCQTADELWDYIETYVTKRSDLPYEIDGIVIKVAGYEEQEQLGFTAKSPR
CCEEECCCCCCCCCHHHHHHHHHHHHHCCCCCCEEECCEEEEEECCCHHHHCCCCCCCCC
WAVAYKFPAEEVVTTIEDVDFSVGRTGKVTPRARFAPVSVAGSTVTYATLHNEDFIKEKD
EEEEEECCHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEECCCEEEEEEECCCCCCCCCC
LHIGDRVVIKKAGDVIPAVVSALASERSGEERVIEFPTHCPACGSELIRLEGEADHRCVN
CCCCCEEEEECCCCHHHHHHHHHHHCCCCCCCEEECCCCCCCCCHHHEEECCCCCCCCCC
PECPAQLLEGLIHFVSRQAMNIDGLGEKVITQLHEAGLVHNVADLYRLDRDDLLGLERMG
CCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCC
ETSVTNLLSAIEQSKQNSLERVLFALGIRLVGQKAASLIAERFETMDAIIAATVEELTSI
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
DGIGTKMAESIVLYFEKPEARELVKELAESGVNLSFKGMKRPTTEGAPLSGKTVVLTGKL
CCCHHHHHCCEEEEECCCHHHHHHHHHHHCCCCEEECCCCCCCCCCCCCCCCEEEEECCH
HEMTRGEAGKQLELLGASVTGSVSKNTDLLVAGEKAGSKLAKAESLGIEVWDEAKLLDFL
HHHHCCCCCCEEEEECCCEECCCCCCCCEEEECCHHCCHHHHHHHCCCEECCHHHHHHHH
KEHA
HHCC
>Mature Secondary Structure
MEMAERVEELRQTLNQYGYEYYVLDRPSVPDSEYDRLMNELIQIETDYPELRTPDSPTQR
CCHHHHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHHEECCCCCCCCCCCCHHHH
VGGAPLDAFVKVTHDTPMLSLGNVFSKEELVEWVERIEKDLGYTPTFVAELKFDGLAVSL
CCCCCHHHEEEEECCCCCHHHCCCCCHHHHHHHHHHHHHHCCCCCEEEEEEEECCEEEEE
KYENGRLVRGATRGDGTTGEDITQNLKTIRSIPLRLNEDVTFEVRGEAYMPKRSFEQLND
EECCCEEEEECCCCCCCCHHHHHHHHHHHHHCCCEECCCEEEEECCCCCCCHHHHHHHHH
ERERIGEPLFANPRNAAAGSLRQLDSKIAASRNLAFFAYGLVSQDDLVASHDESLDYLKR
HHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCHHHCCCHHHHHHHH
LGVAVSGDYTTCQTADELWDYIETYVTKRSDLPYEIDGIVIKVAGYEEQEQLGFTAKSPR
CCEEECCCCCCCCCHHHHHHHHHHHHHCCCCCCEEECCEEEEEECCCHHHHCCCCCCCCC
WAVAYKFPAEEVVTTIEDVDFSVGRTGKVTPRARFAPVSVAGSTVTYATLHNEDFIKEKD
EEEEEECCHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEECCCEEEEEEECCCCCCCCCC
LHIGDRVVIKKAGDVIPAVVSALASERSGEERVIEFPTHCPACGSELIRLEGEADHRCVN
CCCCCEEEEECCCCHHHHHHHHHHHCCCCCCCEEECCCCCCCCCHHHEEECCCCCCCCCC
PECPAQLLEGLIHFVSRQAMNIDGLGEKVITQLHEAGLVHNVADLYRLDRDDLLGLERMG
CCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCC
ETSVTNLLSAIEQSKQNSLERVLFALGIRLVGQKAASLIAERFETMDAIIAATVEELTSI
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
DGIGTKMAESIVLYFEKPEARELVKELAESGVNLSFKGMKRPTTEGAPLSGKTVVLTGKL
CCCHHHHHCCEEEEECCCHHHHHHHHHHHCCCCEEECCCCCCCCCCCCCCCCEEEEECCH
HEMTRGEAGKQLELLGASVTGSVSKNTDLLVAGEKAGSKLAKAESLGIEVWDEAKLLDFL
HHHHCCCCCCEEEEECCCEECCCCCCCCEEEECCHHCCHHHHHHHCCCEECCHHHHHHHH
KEHA
HHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA