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The map label for this gene is purM

Identifier: 229917952

GI number: 229917952

Start: 2178989

End: 2180023

Strand: Direct

Name: purM

Synonym: EAT1b_2231

Alternate gene names: 229917952

Gene position: 2178989-2180023 (Clockwise)

Preceding gene: 229917951

Following gene: 229917953

Centisome position: 72.64

GC content: 54.49

Gene sequence:

>1035_bases
ATGAGCGTTCGTTATGAACAAGCGGGTGTGAATTTAGAAGCAGGGTACGAGGCCGTATCCCGTATGAAACGTCATGTCGC
TCGGACAATGCGTCCGGAAGTGATGACAGGACTCGGTAGTTTCGGAGCGATGATTGACCTCGGTTCGATGAACATGAAAC
ACCCGATTCTCGTGAGCGGGACTGACGGGGTCGGGACGAAACTGAAATTGGCGTTCGCGCTCGACCAACACGACACGATT
GGAATCGATTGCGTCGCCATGTGCGTCAACGATTGTCTCGTGCATGGAGCAGAACCGCTCTATTTCCTCGACTATATTGC
GACCGGGAAAGCAGAACCGGCCAAACTGGAGCGAATCGTCGCCGGTGTTGCAGAAGGGTGTGTCCAAGCCGGTTGTGCGC
TCGTCGGTGGCGAAACGGCTGAGATGCCAGGGATGTATCCGGATGGCGAATATGACATCGCCGGATTTGCGGTCGGTGTC
GTAGAGAAAGAAGACTTGCTCGACGGTTCGACGATTCAGGATGGGGATGTCGTGCTCGGACTTGCCTCTTCAGGCGTCCA
CTCGAACGGGTTCTCCCTTGTACGTCATATCGTGGAAGCGCAAGGTCTTCATTATACGGATGAGATTGCGATGCTCGATA
CGACACTCGGGAACGCCTTGCTGTTGCCGACACGGATTTATGCCGAAGCGGCGAAAGCGGCCATTTCGACAGGCAAGGTT
CAAGGGATGGCACACATCACAGGTGGTGGCTTCTATGAGAATGTGCCTCGCATGTTGCCAGGAGGTCTTGGCATCACGTT
CGATGCGTCGAGTTGGCCGAGTCTTCCTGTATTCGACTGGCTCGAACAAGTCGGGAACATCTCGAAGCAAGAAATGTTCA
ACGTCTTCAATATGGGAATCGGGTATATGATCACGGTACGAGCTGAAGATGTGGAACTCGTCGAAGCGGCGCTTGAACGT
GTCGGGGAGACTGCGCACCGAATCGGGAAAGTCGAGAGTCGTCCAGGCATCCGGATTTCAGGAGTGGACCAATGA

Upstream 100 bases:

>100_bases
AGGAGCACTACGCGGGCAGTGTGCTGCATGCTTCACGGGACTTTATCCGACTGAACTTGCAGAACTCGTGACAGAGACAA
AGGCTTAAGGGGGAAAAGCG

Downstream 100 bases:

>100_bases
AACGATTTGCCATCTTTGCGTCAGGATCGGGAAGTAACGCCGAAGCGATCTGGCAAGCGATCGCCGATGGTCAATTGTCT
GCCGAATGTGTGTTGCTCGT

Product: phosphoribosylaminoimidazole synthetase

Products: NA

Alternate protein names: AIR synthase; AIRS; Phosphoribosyl-aminoimidazole synthetase

Number of amino acids: Translated: 344; Mature: 343

Protein sequence:

>344_residues
MSVRYEQAGVNLEAGYEAVSRMKRHVARTMRPEVMTGLGSFGAMIDLGSMNMKHPILVSGTDGVGTKLKLAFALDQHDTI
GIDCVAMCVNDCLVHGAEPLYFLDYIATGKAEPAKLERIVAGVAEGCVQAGCALVGGETAEMPGMYPDGEYDIAGFAVGV
VEKEDLLDGSTIQDGDVVLGLASSGVHSNGFSLVRHIVEAQGLHYTDEIAMLDTTLGNALLLPTRIYAEAAKAAISTGKV
QGMAHITGGGFYENVPRMLPGGLGITFDASSWPSLPVFDWLEQVGNISKQEMFNVFNMGIGYMITVRAEDVELVEAALER
VGETAHRIGKVESRPGIRISGVDQ

Sequences:

>Translated_344_residues
MSVRYEQAGVNLEAGYEAVSRMKRHVARTMRPEVMTGLGSFGAMIDLGSMNMKHPILVSGTDGVGTKLKLAFALDQHDTI
GIDCVAMCVNDCLVHGAEPLYFLDYIATGKAEPAKLERIVAGVAEGCVQAGCALVGGETAEMPGMYPDGEYDIAGFAVGV
VEKEDLLDGSTIQDGDVVLGLASSGVHSNGFSLVRHIVEAQGLHYTDEIAMLDTTLGNALLLPTRIYAEAAKAAISTGKV
QGMAHITGGGFYENVPRMLPGGLGITFDASSWPSLPVFDWLEQVGNISKQEMFNVFNMGIGYMITVRAEDVELVEAALER
VGETAHRIGKVESRPGIRISGVDQ
>Mature_343_residues
SVRYEQAGVNLEAGYEAVSRMKRHVARTMRPEVMTGLGSFGAMIDLGSMNMKHPILVSGTDGVGTKLKLAFALDQHDTIG
IDCVAMCVNDCLVHGAEPLYFLDYIATGKAEPAKLERIVAGVAEGCVQAGCALVGGETAEMPGMYPDGEYDIAGFAVGVV
EKEDLLDGSTIQDGDVVLGLASSGVHSNGFSLVRHIVEAQGLHYTDEIAMLDTTLGNALLLPTRIYAEAAKAAISTGKVQ
GMAHITGGGFYENVPRMLPGGLGITFDASSWPSLPVFDWLEQVGNISKQEMFNVFNMGIGYMITVRAEDVELVEAALERV
GETAHRIGKVESRPGIRISGVDQ

Specific function: De novo purine biosynthesis; fifth step. [C]

COG id: COG0150

COG function: function code F; Phosphoribosylaminoimidazole (AIR) synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AIR synthase family

Homologues:

Organism=Homo sapiens, GI4503915, Length=336, Percent_Identity=48.8095238095238, Blast_Score=321, Evalue=7e-88,
Organism=Homo sapiens, GI209869995, Length=336, Percent_Identity=48.8095238095238, Blast_Score=321, Evalue=7e-88,
Organism=Homo sapiens, GI209869993, Length=336, Percent_Identity=48.8095238095238, Blast_Score=321, Evalue=7e-88,
Organism=Escherichia coli, GI1788845, Length=333, Percent_Identity=51.9519519519519, Blast_Score=341, Evalue=3e-95,
Organism=Caenorhabditis elegans, GI17567511, Length=350, Percent_Identity=40.2857142857143, Blast_Score=249, Evalue=1e-66,
Organism=Saccharomyces cerevisiae, GI6321203, Length=340, Percent_Identity=44.7058823529412, Blast_Score=286, Evalue=3e-78,
Organism=Drosophila melanogaster, GI24582400, Length=297, Percent_Identity=43.7710437710438, Blast_Score=231, Evalue=8e-61,

Paralogues:

None

Copy number: 180 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): PUR5_EXISA (C4L2A1)

Other databases:

- EMBL:   CP001615
- RefSeq:   YP_002886598.1
- GeneID:   7870818
- GenomeReviews:   CP001615_GR
- KEGG:   eat:EAT1b_2231
- OMA:   HYDLAGF
- ProtClustDB:   PRK05385
- GO:   GO:0005737
- HAMAP:   MF_00741_B
- InterPro:   IPR000728
- InterPro:   IPR010918
- InterPro:   IPR004733
- InterPro:   IPR016188
- TIGRFAMs:   TIGR00878

Pfam domain/function: PF00586 AIRS; PF02769 AIRS_C; SSF56042 AIR_synth_C; SSF55326 PurM_N-like

EC number: =6.3.3.1

Molecular weight: Translated: 36585; Mature: 36454

Theoretical pI: Translated: 4.55; Mature: 4.55

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVRYEQAGVNLEAGYEAVSRMKRHVARTMRPEVMTGLGSFGAMIDLGSMNMKHPILVSG
CCCEEHHCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHCCHHHEEEECCCCCCCCEEEEC
TDGVGTKLKLAFALDQHDTIGIDCVAMCVNDCLVHGAEPLYFLDYIATGKAEPAKLERIV
CCCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHCCCCHHEEEHHHHCCCCCHHHHHHHH
AGVAEGCVQAGCALVGGETAEMPGMYPDGEYDIAGFAVGVVEKEDLLDGSTIQDGDVVLG
HHHHHHHHHHCCEEECCCCCCCCCCCCCCCEEECEEEEEEEEHHHCCCCCCCCCCCEEEE
LASSGVHSNGFSLVRHIVEAQGLHYTDEIAMLDTTLGNALLLPTRIYAEAAKAAISTGKV
EECCCCCCCHHHHHHHHHHHCCCCCCCHHHEEHHCCCCEEEEHHHHHHHHHHHHHHHCCC
QGMAHITGGGFYENVPRMLPGGLGITFDASSWPSLPVFDWLEQVGNISKQEMFNVFNMGI
CCEEEECCCCHHHCCHHHCCCCCEEEECCCCCCCCCHHHHHHHHCCCCHHHHHHHHHCCC
GYMITVRAEDVELVEAALERVGETAHRIGKVESRPGIRISGVDQ
CEEEEEECCHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCC
>Mature Secondary Structure 
SVRYEQAGVNLEAGYEAVSRMKRHVARTMRPEVMTGLGSFGAMIDLGSMNMKHPILVSG
CCEEHHCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHCCHHHEEEECCCCCCCCEEEEC
TDGVGTKLKLAFALDQHDTIGIDCVAMCVNDCLVHGAEPLYFLDYIATGKAEPAKLERIV
CCCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHCCCCHHEEEHHHHCCCCCHHHHHHHH
AGVAEGCVQAGCALVGGETAEMPGMYPDGEYDIAGFAVGVVEKEDLLDGSTIQDGDVVLG
HHHHHHHHHHCCEEECCCCCCCCCCCCCCCEEECEEEEEEEEHHHCCCCCCCCCCCEEEE
LASSGVHSNGFSLVRHIVEAQGLHYTDEIAMLDTTLGNALLLPTRIYAEAAKAAISTGKV
EECCCCCCCHHHHHHHHHHHCCCCCCCHHHEEHHCCCCEEEEHHHHHHHHHHHHHHHCCC
QGMAHITGGGFYENVPRMLPGGLGITFDASSWPSLPVFDWLEQVGNISKQEMFNVFNMGI
CCEEEECCCCHHHCCHHHCCCCCEEEECCCCCCCCCHHHHHHHHCCCCHHHHHHHHHCCC
GYMITVRAEDVELVEAALERVGETAHRIGKVESRPGIRISGVDQ
CEEEEEECCHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA