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The map label for this gene is pbuG [H]

Identifier: 229917940

GI number: 229917940

Start: 2156840

End: 2158201

Strand: Direct

Name: pbuG [H]

Synonym: EAT1b_2219

Alternate gene names: 229917940

Gene position: 2156840-2158201 (Clockwise)

Preceding gene: 229917939

Following gene: 229917942

Centisome position: 71.9

GC content: 50.0

Gene sequence:

>1362_bases
ATGAGCACACAGCTCAAGCAAGAACAGCAACAACCATCAACAACGAGTCGGATCGATCGTTACTTCCAACTCACAGCACT
CGGTACGAGTGTCCGCACGGAACTGATCGCAGGATTTACAACTTTCCTTGCGATGGCTTACATCCTCTTCGTTAACCCGA
ACATCCTTGGGGCAGCGGGTATGGATACAGGAGCGGTCTTCGTGGCGACAGGTCTTGTTGCAGTAGTCGGCTCATTGACG
ATGGGACTTCTCGCGAACTACCCGATTGCGATTGCGCCAGGCATGGGCCTTAACGCGTTCTTCGCATATAGCGTCGTCAT
CGGGATGGGTATTTCTTGGCAAACGGCTTTGTCAGGTGTACTTGTATCAGGTCTCTTCTTTATCGTATTGACGTTATCAG
GAATTCGCGAAACGATTGTTAATGCGATTCCGGCTCCGCTCAAAATGGCGGTTGCTGCAGGGATTGGTTTCTTCATCGCT
TTCATCGGTTTGAAAAACGCAGGCATCGTTGTCGCGAATGAAGCAACACTTGTTAGTCTTGGTTCGCTCGGAACGGGCAC
GACGCTTCTCGCGGTGTTCGGTCTTGTGGTCAGCGCCTTGCTTATGGCGCGCGACATCAAAGGTGGTATTTTCATCGGGA
TGTTGTTGACGGCGATTGTCGGGATGATCTTCGGTCTCGTGCCAACTCCGACAGCAATCTCAGACGTTGTATCCATGCCG
CCGAGTATGTCATCTACATTCGGTCAAGCCTTCATCAACTTCGGTGATGTGTTCACGCTTCAAATGCTCGTTGTCATCTT
GACGTTCTTCTTCATCGACTTCTTTGATACAGCTGGGACGCTCGTCGCGGTAGCGCGTCAAGCTGGGATCATGAAAGAGA
ACAAAGTACCACGTGCGAGTAAGGCGTTGTTAGCTGACTCGACAGCGACGGTGGCAGGTGCAATCTTCGGAACATCTACA
GCGACTTCATACGTTGAATCGTCAGCAGGTGTCGCTGCTGGTGGACGAACAGGATTGACTGCTGTTTTCGCAGGATTGTT
CTTCGGTTTGGCATTGTTCTTCTCGCCTCTCTTGGCAGTTGTTACGGCGCCGGTCACAGCTCCTGCGCTGATTATTGTTG
GTGTGCTCATGGCTTCGGCTCTTCTTGATATCGATTGGAAAAAGATCGAGATTGCAGTTCCTGCATTCTTGACGATTATC
ATGATGCCACTTACATCATCAATCGCAACGGGAATTGGGCTTGGGTTTATCCTTTACCCACTCATGATGATTGCAAAAGG
GAAGACGAAAGAGATTCATCCGATCATGTATGGGATGTTGGTCATCTTCTTGGCATACTTCATGTTCTTGCCACACGCAT
AA

Upstream 100 bases:

>100_bases
CATAGATGAGGGTGTATGCCCTCGTATGTTTCGCGTGGGGTCTTCGGTCATTTGCCGGGGACCTTTTCTCGTTTTACGGC
GAGCACAAGGAGGAAAACAA

Downstream 100 bases:

>100_bases
GTTCATTTGAAAAGCCGGCTCTATTATTACGAGTCGGCTTTTTTGTATGCAGTATTTTCAGGAAGGAATGGATCGTACAT
CGTGATCGCGTGATTTTCTG

Product: Xanthine/uracil/vitamin C permease

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 453; Mature: 452

Protein sequence:

>453_residues
MSTQLKQEQQQPSTTSRIDRYFQLTALGTSVRTELIAGFTTFLAMAYILFVNPNILGAAGMDTGAVFVATGLVAVVGSLT
MGLLANYPIAIAPGMGLNAFFAYSVVIGMGISWQTALSGVLVSGLFFIVLTLSGIRETIVNAIPAPLKMAVAAGIGFFIA
FIGLKNAGIVVANEATLVSLGSLGTGTTLLAVFGLVVSALLMARDIKGGIFIGMLLTAIVGMIFGLVPTPTAISDVVSMP
PSMSSTFGQAFINFGDVFTLQMLVVILTFFFIDFFDTAGTLVAVARQAGIMKENKVPRASKALLADSTATVAGAIFGTST
ATSYVESSAGVAAGGRTGLTAVFAGLFFGLALFFSPLLAVVTAPVTAPALIIVGVLMASALLDIDWKKIEIAVPAFLTII
MMPLTSSIATGIGLGFILYPLMMIAKGKTKEIHPIMYGMLVIFLAYFMFLPHA

Sequences:

>Translated_453_residues
MSTQLKQEQQQPSTTSRIDRYFQLTALGTSVRTELIAGFTTFLAMAYILFVNPNILGAAGMDTGAVFVATGLVAVVGSLT
MGLLANYPIAIAPGMGLNAFFAYSVVIGMGISWQTALSGVLVSGLFFIVLTLSGIRETIVNAIPAPLKMAVAAGIGFFIA
FIGLKNAGIVVANEATLVSLGSLGTGTTLLAVFGLVVSALLMARDIKGGIFIGMLLTAIVGMIFGLVPTPTAISDVVSMP
PSMSSTFGQAFINFGDVFTLQMLVVILTFFFIDFFDTAGTLVAVARQAGIMKENKVPRASKALLADSTATVAGAIFGTST
ATSYVESSAGVAAGGRTGLTAVFAGLFFGLALFFSPLLAVVTAPVTAPALIIVGVLMASALLDIDWKKIEIAVPAFLTII
MMPLTSSIATGIGLGFILYPLMMIAKGKTKEIHPIMYGMLVIFLAYFMFLPHA
>Mature_452_residues
STQLKQEQQQPSTTSRIDRYFQLTALGTSVRTELIAGFTTFLAMAYILFVNPNILGAAGMDTGAVFVATGLVAVVGSLTM
GLLANYPIAIAPGMGLNAFFAYSVVIGMGISWQTALSGVLVSGLFFIVLTLSGIRETIVNAIPAPLKMAVAAGIGFFIAF
IGLKNAGIVVANEATLVSLGSLGTGTTLLAVFGLVVSALLMARDIKGGIFIGMLLTAIVGMIFGLVPTPTAISDVVSMPP
SMSSTFGQAFINFGDVFTLQMLVVILTFFFIDFFDTAGTLVAVARQAGIMKENKVPRASKALLADSTATVAGAIFGTSTA
TSYVESSAGVAAGGRTGLTAVFAGLFFGLALFFSPLLAVVTAPVTAPALIIVGVLMASALLDIDWKKIEIAVPAFLTIIM
MPLTSSIATGIGLGFILYPLMMIAKGKTKEIHPIMYGMLVIFLAYFMFLPHA

Specific function: Involved in the uptake of the purine bases hypoxanthine and guanine [H]

COG id: COG2252

COG function: function code R; Permeases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the xanthine/uracil permease family. AzgA purine transporter (TC 2.A.1.40) subfamily [H]

Homologues:

Organism=Escherichia coli, GI87082309, Length=429, Percent_Identity=45.2214452214452, Blast_Score=363, Evalue=1e-101,
Organism=Escherichia coli, GI1790150, Length=446, Percent_Identity=43.7219730941704, Blast_Score=355, Evalue=2e-99,
Organism=Escherichia coli, GI1790499, Length=442, Percent_Identity=37.5565610859729, Blast_Score=229, Evalue=3e-61,
Organism=Escherichia coli, GI48994909, Length=455, Percent_Identity=36.4835164835165, Blast_Score=224, Evalue=6e-60,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006043 [H]

Pfam domain/function: PF00860 Xan_ur_permease [H]

EC number: NA

Molecular weight: Translated: 47147; Mature: 47016

Theoretical pI: Translated: 9.20; Mature: 9.20

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.9 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.6 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTQLKQEQQQPSTTSRIDRYFQLTALGTSVRTELIAGFTTFLAMAYILFVNPNILGAAG
CCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCC
MDTGAVFVATGLVAVVGSLTMGLLANYPIAIAPGMGLNAFFAYSVVIGMGISWQTALSGV
CCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHHCCCCHHHHHHHH
LVSGLFFIVLTLSGIRETIVNAIPAPLKMAVAAGIGFFIAFIGLKNAGIVVANEATLVSL
HHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCEEEEE
GSLGTGTTLLAVFGLVVSALLMARDIKGGIFIGMLLTAIVGMIFGLVPTPTAISDVVSMP
CCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCC
PSMSSTFGQAFINFGDVFTLQMLVVILTFFFIDFFDTAGTLVAVARQAGIMKENKVPRAS
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHH
KALLADSTATVAGAIFGTSTATSYVESSAGVAAGGRTGLTAVFAGLFFGLALFFSPLLAV
HHHHHCCHHHHHHHHHCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
VTAPVTAPALIIVGVLMASALLDIDWKKIEIAVPAFLTIIMMPLTSSIATGIGLGFILYP
HHCCCHHHHHHHHHHHHHHHHHCCCCHHEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LMMIAKGKTKEIHPIMYGMLVIFLAYFMFLPHA
HHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
STQLKQEQQQPSTTSRIDRYFQLTALGTSVRTELIAGFTTFLAMAYILFVNPNILGAAG
CCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCC
MDTGAVFVATGLVAVVGSLTMGLLANYPIAIAPGMGLNAFFAYSVVIGMGISWQTALSGV
CCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHHCCCCHHHHHHHH
LVSGLFFIVLTLSGIRETIVNAIPAPLKMAVAAGIGFFIAFIGLKNAGIVVANEATLVSL
HHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCEEEEE
GSLGTGTTLLAVFGLVVSALLMARDIKGGIFIGMLLTAIVGMIFGLVPTPTAISDVVSMP
CCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCC
PSMSSTFGQAFINFGDVFTLQMLVVILTFFFIDFFDTAGTLVAVARQAGIMKENKVPRAS
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHH
KALLADSTATVAGAIFGTSTATSYVESSAGVAAGGRTGLTAVFAGLFFGLALFFSPLLAV
HHHHHCCHHHHHHHHHCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
VTAPVTAPALIIVGVLMASALLDIDWKKIEIAVPAFLTIIMMPLTSSIATGIGLGFILYP
HHCCCHHHHHHHHHHHHHHHHHCCCCHHEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LMMIAKGKTKEIHPIMYGMLVIFLAYFMFLPHA
HHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8969499; 9384377; 11591660 [H]