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The map label for this gene is vanS [H]

Identifier: 229917897

GI number: 229917897

Start: 2111088

End: 2112176

Strand: Direct

Name: vanS [H]

Synonym: EAT1b_2175

Alternate gene names: 229917897

Gene position: 2111088-2112176 (Clockwise)

Preceding gene: 229917896

Following gene: 229917900

Centisome position: 70.37

GC content: 44.17

Gene sequence:

>1089_bases
ATGAGACGGAGACAATGGGATCGCTTGCTAGTGAAGTTGATTGGTATCATTTTAGTCAATGCATTGATTGCGACGATTGC
TTTTTTAATCATGGGCTATTCGATCAGTTTATACTACATTGAGAACGAAACAACACCAGATCCGCTGACGTCCAATCTTA
TGTTTTTAGGGGTGACGTTCATTGTTATTTTGATCTTTATTATTGGATTTTCCCTGATGATGCGGAAAACACTTCGAAAA
ATCACGTATTTATCGAGTGAGATTGAACAGATTGCAAACGGTGACCTCGGTCGAACTGTCAAAGTGGAGGGTCGAGACGA
AATCGCGTCGCTTGGAGAGAGTGTGAATCGGATGTCAGCTCAATTAAGTGAATTATTTGAGAAGGAACGGGCGCATGAAG
AAGAGCGGAATCAACTATTCTCCAACCTGTCGCACGATTTGCGGACACCGCTCACGAGCATCAAAGGCTATACGCAACTC
CTGCAAATGAACCGCGAGCTGACAGCTGAAGAGAACAATCATTTTCGTATTCTGAATGAAAAAACGGCGCAGCTTGAACA
TCTACTCGATCAGTTGATGGACGTGAACCGCCTCTATGCCCCACACATGCAACTAAAAAAACAGAAACTCAACTTGAGTT
TGTTGACGACCCAGCTCATCCACGAGATGGAGCCGTTATTTAAGGAAAGAGGTCATCGATTACACCTGTCGATTCAATCT
GACCGATGGATAGAGGCTGATCAAGAACAGCTCATTCGTGTATTTGAAAATCTTTTCTCGAATACTTGGAAATACGCCGA
GCAAACGAGTCCAATCGAGATTAGACTTCAAACGAAAGGACAACAGATTGAATGGTCTATTTCAAATGCGACAACGGCTG
AAACGATTGAAGCCATCCCCCATCTCTTTGATCGCACGTATCGCGTCGATTCGTCGAGAGGGGTCACTCCAGGTGATGGA
TTGGGTCTATCGATTGCCCGTCAAATCATTTTGTTACATGGAGGACAGTTGACAGCGGAACCGCTAGAGGGTCAGATCAT
TTGTTTTCGGGTATCGTTTCCGACTCATCAACATATAGAACGTGAGTAA

Upstream 100 bases:

>100_bases
ACACAGTGATGGTCCACATCACAAAAATCCGAGAAAAGTTAGAAGACACACCACGAAAACCGACCATTATCAAGACGGTG
TGGGGCGTGGGGTATCGATT

Downstream 100 bases:

>100_bases
ATAGCTGTCCTTCGTTTTGAAAGTCGTAGTTCATAAAAGGCTGCGACTTTTTGTGTCGAGCAAATAACCTCAAAAAAAAA
GCACTCGGACCATTGTCCGA

Product: histidine kinase

Products: NA

Alternate protein names: Vancomycin histidine protein kinase; Vancomycin resistance protein vanS [H]

Number of amino acids: Translated: 362; Mature: 362

Protein sequence:

>362_residues
MRRRQWDRLLVKLIGIILVNALIATIAFLIMGYSISLYYIENETTPDPLTSNLMFLGVTFIVILIFIIGFSLMMRKTLRK
ITYLSSEIEQIANGDLGRTVKVEGRDEIASLGESVNRMSAQLSELFEKERAHEEERNQLFSNLSHDLRTPLTSIKGYTQL
LQMNRELTAEENNHFRILNEKTAQLEHLLDQLMDVNRLYAPHMQLKKQKLNLSLLTTQLIHEMEPLFKERGHRLHLSIQS
DRWIEADQEQLIRVFENLFSNTWKYAEQTSPIEIRLQTKGQQIEWSISNATTAETIEAIPHLFDRTYRVDSSRGVTPGDG
LGLSIARQIILLHGGQLTAEPLEGQIICFRVSFPTHQHIERE

Sequences:

>Translated_362_residues
MRRRQWDRLLVKLIGIILVNALIATIAFLIMGYSISLYYIENETTPDPLTSNLMFLGVTFIVILIFIIGFSLMMRKTLRK
ITYLSSEIEQIANGDLGRTVKVEGRDEIASLGESVNRMSAQLSELFEKERAHEEERNQLFSNLSHDLRTPLTSIKGYTQL
LQMNRELTAEENNHFRILNEKTAQLEHLLDQLMDVNRLYAPHMQLKKQKLNLSLLTTQLIHEMEPLFKERGHRLHLSIQS
DRWIEADQEQLIRVFENLFSNTWKYAEQTSPIEIRLQTKGQQIEWSISNATTAETIEAIPHLFDRTYRVDSSRGVTPGDG
LGLSIARQIILLHGGQLTAEPLEGQIICFRVSFPTHQHIERE
>Mature_362_residues
MRRRQWDRLLVKLIGIILVNALIATIAFLIMGYSISLYYIENETTPDPLTSNLMFLGVTFIVILIFIIGFSLMMRKTLRK
ITYLSSEIEQIANGDLGRTVKVEGRDEIASLGESVNRMSAQLSELFEKERAHEEERNQLFSNLSHDLRTPLTSIKGYTQL
LQMNRELTAEENNHFRILNEKTAQLEHLLDQLMDVNRLYAPHMQLKKQKLNLSLLTTQLIHEMEPLFKERGHRLHLSIQS
DRWIEADQEQLIRVFENLFSNTWKYAEQTSPIEIRLQTKGQQIEWSISNATTAETIEAIPHLFDRTYRVDSSRGVTPGDG
LGLSIARQIILLHGGQLTAEPLEGQIICFRVSFPTHQHIERE

Specific function: Member of the two-component regulatory system vanS/vanR. Activates the transcription of vanH, vanA and vanX in response to vancomycin which results in vancomycin resistance. VanS activates vanR by phosphorylation [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI87082128, Length=309, Percent_Identity=28.8025889967638, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI1788393, Length=292, Percent_Identity=24.6575342465753, Blast_Score=95, Evalue=9e-21,
Organism=Escherichia coli, GI1786912, Length=236, Percent_Identity=31.3559322033898, Blast_Score=92, Evalue=4e-20,
Organism=Escherichia coli, GI1786783, Length=350, Percent_Identity=25.7142857142857, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI1786600, Length=223, Percent_Identity=26.9058295964126, Blast_Score=89, Evalue=4e-19,
Organism=Escherichia coli, GI1787894, Length=308, Percent_Identity=24.3506493506494, Blast_Score=88, Evalue=8e-19,
Organism=Escherichia coli, GI1788279, Length=300, Percent_Identity=27, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI48994928, Length=230, Percent_Identity=23.4782608695652, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI87081816, Length=337, Percent_Identity=23.7388724035608, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1789149, Length=348, Percent_Identity=22.9885057471264, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI145693157, Length=245, Percent_Identity=22.0408163265306, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI1789808, Length=282, Percent_Identity=24.822695035461, Blast_Score=65, Evalue=5e-12,
Organism=Escherichia coli, GI1788713, Length=265, Percent_Identity=23.0188679245283, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 41836; Mature: 41836

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS50885 HAMP ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRRQWDRLLVKLIGIILVNALIATIAFLIMGYSISLYYIENETTPDPLTSNLMFLGVTF
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCHHHHHHHHHHHH
IVILIFIIGFSLMMRKTLRKITYLSSEIEQIANGDLGRTVKVEGRDEIASLGESVNRMSA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCHHHHHHHHHHHHHHHH
QLSELFEKERAHEEERNQLFSNLSHDLRTPLTSIKGYTQLLQMNRELTAEENNHFRILNE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECH
KTAQLEHLLDQLMDVNRLYAPHMQLKKQKLNLSLLTTQLIHEMEPLFKERGHRLHLSIQS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECC
DRWIEADQEQLIRVFENLFSNTWKYAEQTSPIEIRLQTKGQQIEWSISNATTAETIEAIP
CCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCEEEEEECCCCHHHHHHHHH
HLFDRTYRVDSSRGVTPGDGLGLSIARQIILLHGGQLTAEPLEGQIICFRVSFPTHQHIE
HHHHHHEEECCCCCCCCCCCCCHHHHHHHHHHCCCCEECCCCCCCEEEEEECCCCHHCCC
RE
CC
>Mature Secondary Structure
MRRRQWDRLLVKLIGIILVNALIATIAFLIMGYSISLYYIENETTPDPLTSNLMFLGVTF
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCHHHHHHHHHHHH
IVILIFIIGFSLMMRKTLRKITYLSSEIEQIANGDLGRTVKVEGRDEIASLGESVNRMSA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCHHHHHHHHHHHHHHHH
QLSELFEKERAHEEERNQLFSNLSHDLRTPLTSIKGYTQLLQMNRELTAEENNHFRILNE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECH
KTAQLEHLLDQLMDVNRLYAPHMQLKKQKLNLSLLTTQLIHEMEPLFKERGHRLHLSIQS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECC
DRWIEADQEQLIRVFENLFSNTWKYAEQTSPIEIRLQTKGQQIEWSISNATTAETIEAIP
CCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCEEEEEECCCCHHHHHHHHH
HLFDRTYRVDSSRGVTPGDGLGLSIARQIILLHGGQLTAEPLEGQIICFRVSFPTHQHIE
HHHHHHEEECCCCCCCCCCCCCHHHHHHHHHHCCCCEECCCCCCCEEEEEECCCCHHCCC
RE
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8380148; 1556077 [H]