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The map label for this gene is groEL

Identifier: 229917843

GI number: 229917843

Start: 2069574

End: 2071208

Strand: Direct

Name: groEL

Synonym: EAT1b_2121

Alternate gene names: 229917843

Gene position: 2069574-2071208 (Clockwise)

Preceding gene: 229917842

Following gene: 229917844

Centisome position: 68.99

GC content: 52.78

Gene sequence:

>1635_bases
ATGGCAAAAGATATTAAATTCTCAGAAGATGCACGTCACGCGATGCTTCGCGGCGTCGACGCACTCGCTGACGCAGTCAA
AGTAACGATTGGACCAAAAGGACGCAACGTTGTCCTCGAGAAGAAATTCGGTTCACCACTCATCACAAACGATGGTGTGA
CAATCGCGAAAGAAATCGAACTCGAAGACCGCTTCGAAAACATGGGCGCGAAACTCGTTGCGGAAGTCGCGTCGAAGACG
AACGAAGTCGCAGGGGACGGTACGACAACAGCGACAGTCCTCGCACAAGCGATGATTCGTGAAGGACTCAAGAACGTCAC
GGCGGGCGCGAACCCGATGATCCTTCGTAAAGGAATCGACAAAGCGGTCACACGTGCGCTTGAAGAGTTGAAAGCCATCT
CGAAACCAATCGAGTCAAAAGAAGCGATCGCACAAGTTGCGGCCATCTCGGCAGCGGACGAAGAAGTTGGCGAATTGATC
GCAGAAGCGATGGAGCGCGTCGGTAACGATGGTGTCATCACAATCGAAGAATCACGCGGCTTCACGACTGAGCTCGACGT
TGTCGAAGGGATGCAGTTCGACCGTGGATACTTGTCACCATACATGATCTCAGACTCTGACAAGATGGAAGCGTCACTCG
ACAATCCGTACATCTTGATCACAGACAAGAAGATCTCAAACATTCAAGAAATCATCCCAGTGCTCGAGCAAGTCGTGCAA
CAAGGGAAGCCGATCTTGATCGTCGCAGAAGATATCGAAGGCGATGCCCTCGCGACACTCGTCTTGAACAAACTCCGTGG
TACGTTCAACGCGGTAGCGGTCAAAGCACCAGGATTCGGTGACCGTCGTAAAGCGATGCTTGAAGACCTCGCGACACTCA
CAGGTGGTCAAGTTATCACAGAAGACCTCGGTCTCGACCTCAAATCGGCATCACTCGATATGCTCGGTCGCGCATCGAAA
GTCGTCGTCACAAAAGACACAACGACTGTCGTCGAAGGTGCAGGGAACGAAGAAACAATCAAAGCACGCGTTCAAACGAT
TCGTAACCAAATCGAAGAGACAACGTCTGATTTCGACCGTGAAAAACTCCAAGAGCGTCTCGCGAAACTCGCAGGTGGCG
TAGCCGTCGTCAAAGTCGGTGCAGCTACGGAGACTGAGCTTAAAGAGCGTAAACTCCGCATCGAAGACGCACTCAACGCG
ACACGTGCAGCAGTCGAAGAAGGAATCGTGGCGGGTGGTGGTACATCACTCATCAACGTCATCCCAGCCGTTCGTGCGTT
GCTTTCAGAAGTGACAGCGGATGAAGCGACTGGTGTCAAACTCGTCCTTCGTGCACTCGAAGCACCGGTCCGTCAAATCG
CGGAAAACGCGGGTGAAGAAGGTTCGGTCATCGTCGAGAAACTCAAGAACGAGTCAGTCGGTGTCGGCTACAACGCGGCA
ACTGGCGAATATGTCGACATGATCGCACATGGTATCGTCGACCCAGCGAAAGTGACGCGTTCAGCACTCCAAAACGCAGC
ATCTGTCTCAGCGATGTTCCTCACAACAGAGGCCGTCATCGCAGACAAGCCAGAAAAAGACGCTCCTGCAATGCCTGACA
TGGGCGGCATGGGTGGAATGGGCGGTATGATGTAA

Upstream 100 bases:

>100_bases
GCATGATGGAAAAGAGTACTTAATCGTTCGCGAGAGCGATATTCTCGCAATCGTAGGCTAAGCACCATTATTCAAAAACT
TTTTTAGGGGGAAATGAACG

Downstream 100 bases:

>100_bases
GTCATGCGCCTTCACAGGAACCATTCATCAGCGAAAGCTGGTGGGTGGTTTTTTTGTTGTCAGTGATGATTCAAAATGAT
TAAAGTCAGTTAACTACTCT

Product: chaperonin GroEL

Products: NA

Alternate protein names: GroEL protein; Protein Cpn60

Number of amino acids: Translated: 544; Mature: 543

Protein sequence:

>544_residues
MAKDIKFSEDARHAMLRGVDALADAVKVTIGPKGRNVVLEKKFGSPLITNDGVTIAKEIELEDRFENMGAKLVAEVASKT
NEVAGDGTTTATVLAQAMIREGLKNVTAGANPMILRKGIDKAVTRALEELKAISKPIESKEAIAQVAAISAADEEVGELI
AEAMERVGNDGVITIEESRGFTTELDVVEGMQFDRGYLSPYMISDSDKMEASLDNPYILITDKKISNIQEIIPVLEQVVQ
QGKPILIVAEDIEGDALATLVLNKLRGTFNAVAVKAPGFGDRRKAMLEDLATLTGGQVITEDLGLDLKSASLDMLGRASK
VVVTKDTTTVVEGAGNEETIKARVQTIRNQIEETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKERKLRIEDALNA
TRAAVEEGIVAGGGTSLINVIPAVRALLSEVTADEATGVKLVLRALEAPVRQIAENAGEEGSVIVEKLKNESVGVGYNAA
TGEYVDMIAHGIVDPAKVTRSALQNAASVSAMFLTTEAVIADKPEKDAPAMPDMGGMGGMGGMM

Sequences:

>Translated_544_residues
MAKDIKFSEDARHAMLRGVDALADAVKVTIGPKGRNVVLEKKFGSPLITNDGVTIAKEIELEDRFENMGAKLVAEVASKT
NEVAGDGTTTATVLAQAMIREGLKNVTAGANPMILRKGIDKAVTRALEELKAISKPIESKEAIAQVAAISAADEEVGELI
AEAMERVGNDGVITIEESRGFTTELDVVEGMQFDRGYLSPYMISDSDKMEASLDNPYILITDKKISNIQEIIPVLEQVVQ
QGKPILIVAEDIEGDALATLVLNKLRGTFNAVAVKAPGFGDRRKAMLEDLATLTGGQVITEDLGLDLKSASLDMLGRASK
VVVTKDTTTVVEGAGNEETIKARVQTIRNQIEETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKERKLRIEDALNA
TRAAVEEGIVAGGGTSLINVIPAVRALLSEVTADEATGVKLVLRALEAPVRQIAENAGEEGSVIVEKLKNESVGVGYNAA
TGEYVDMIAHGIVDPAKVTRSALQNAASVSAMFLTTEAVIADKPEKDAPAMPDMGGMGGMGGMM
>Mature_543_residues
AKDIKFSEDARHAMLRGVDALADAVKVTIGPKGRNVVLEKKFGSPLITNDGVTIAKEIELEDRFENMGAKLVAEVASKTN
EVAGDGTTTATVLAQAMIREGLKNVTAGANPMILRKGIDKAVTRALEELKAISKPIESKEAIAQVAAISAADEEVGELIA
EAMERVGNDGVITIEESRGFTTELDVVEGMQFDRGYLSPYMISDSDKMEASLDNPYILITDKKISNIQEIIPVLEQVVQQ
GKPILIVAEDIEGDALATLVLNKLRGTFNAVAVKAPGFGDRRKAMLEDLATLTGGQVITEDLGLDLKSASLDMLGRASKV
VVTKDTTTVVEGAGNEETIKARVQTIRNQIEETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKERKLRIEDALNAT
RAAVEEGIVAGGGTSLINVIPAVRALLSEVTADEATGVKLVLRALEAPVRQIAENAGEEGSVIVEKLKNESVGVGYNAAT
GEYVDMIAHGIVDPAKVTRSALQNAASVSAMFLTTEAVIADKPEKDAPAMPDMGGMGGMGGMM

Specific function: Prevents misfolding and promotes the refolding and proper assembly of unfolded polypeptides generated under stress conditions

COG id: COG0459

COG function: function code O; Chaperonin GroEL (HSP60 family)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the chaperonin (HSP60) family

Homologues:

Organism=Homo sapiens, GI41399285, Length=531, Percent_Identity=50.6591337099812, Blast_Score=528, Evalue=1e-150,
Organism=Homo sapiens, GI31542947, Length=531, Percent_Identity=50.6591337099812, Blast_Score=528, Evalue=1e-150,
Organism=Homo sapiens, GI38455427, Length=127, Percent_Identity=29.9212598425197, Blast_Score=71, Evalue=2e-12,
Organism=Homo sapiens, GI302058290, Length=513, Percent_Identity=23.0019493177388, Blast_Score=67, Evalue=4e-11,
Organism=Escherichia coli, GI1790586, Length=528, Percent_Identity=64.0151515151515, Blast_Score=669, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17555558, Length=531, Percent_Identity=46.8926553672316, Blast_Score=489, Evalue=1e-138,
Organism=Caenorhabditis elegans, GI193210679, Length=212, Percent_Identity=44.811320754717, Blast_Score=183, Evalue=2e-46,
Organism=Caenorhabditis elegans, GI25144674, Length=174, Percent_Identity=31.0344827586207, Blast_Score=77, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI25147750, Length=549, Percent_Identity=24.9544626593807, Blast_Score=76, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI17564182, Length=539, Percent_Identity=20.4081632653061, Blast_Score=69, Evalue=6e-12,
Organism=Saccharomyces cerevisiae, GI6323288, Length=528, Percent_Identity=52.6515151515151, Blast_Score=528, Evalue=1e-151,
Organism=Saccharomyces cerevisiae, GI6322350, Length=561, Percent_Identity=21.9251336898396, Blast_Score=78, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6322446, Length=229, Percent_Identity=25.764192139738, Blast_Score=70, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6322524, Length=121, Percent_Identity=30.5785123966942, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24641193, Length=529, Percent_Identity=50.0945179584121, Blast_Score=526, Evalue=1e-149,
Organism=Drosophila melanogaster, GI24641191, Length=529, Percent_Identity=50.0945179584121, Blast_Score=526, Evalue=1e-149,
Organism=Drosophila melanogaster, GI45550936, Length=525, Percent_Identity=47.4285714285714, Blast_Score=489, Evalue=1e-138,
Organism=Drosophila melanogaster, GI45550132, Length=525, Percent_Identity=47.4285714285714, Blast_Score=489, Evalue=1e-138,
Organism=Drosophila melanogaster, GI45550935, Length=525, Percent_Identity=47.4285714285714, Blast_Score=489, Evalue=1e-138,
Organism=Drosophila melanogaster, GI17864606, Length=541, Percent_Identity=43.2532347504621, Blast_Score=451, Evalue=1e-127,
Organism=Drosophila melanogaster, GI24584129, Length=537, Percent_Identity=37.6163873370577, Blast_Score=331, Evalue=9e-91,
Organism=Drosophila melanogaster, GI19921262, Length=537, Percent_Identity=37.6163873370577, Blast_Score=331, Evalue=9e-91,
Organism=Drosophila melanogaster, GI28571140, Length=567, Percent_Identity=24.6913580246914, Blast_Score=82, Evalue=1e-15,
Organism=Drosophila melanogaster, GI18858175, Length=567, Percent_Identity=24.6913580246914, Blast_Score=82, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24645179, Length=493, Percent_Identity=21.7038539553753, Blast_Score=81, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24583944, Length=562, Percent_Identity=23.3096085409253, Blast_Score=75, Evalue=1e-13,
Organism=Drosophila melanogaster, GI17647245, Length=552, Percent_Identity=21.9202898550725, Blast_Score=68, Evalue=1e-11,

Paralogues:

None

Copy number: 2180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 480 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 15012 Molecules/Cell In: Growth Phase,

Swissprot (AC and ID): CH60_EXISA (C4L1L2)

Other databases:

- EMBL:   CP001615
- RefSeq:   YP_002886489.1
- GeneID:   7870546
- GenomeReviews:   CP001615_GR
- KEGG:   eat:EAT1b_2121
- OMA:   DKPAAGM
- ProtClustDB:   PRK00013
- GO:   GO:0005737
- HAMAP:   MF_00600
- InterPro:   IPR018370
- InterPro:   IPR001844
- InterPro:   IPR002423
- PANTHER:   PTHR11353
- PRINTS:   PR00298
- TIGRFAMs:   TIGR02348

Pfam domain/function: PF00118 Cpn60_TCP1; SSF48592 GroEL-ATPase

EC number: NA

Molecular weight: Translated: 57609; Mature: 57478

Theoretical pI: Translated: 4.49; Mature: 4.49

Prosite motif: PS00296 CHAPERONINS_CPN60

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKDIKFSEDARHAMLRGVDALADAVKVTIGPKGRNVVLEKKFGSPLITNDGVTIAKEIE
CCCCCCCCHHHHHHHHHHHHHHHHHHEEEECCCCCEEEEEECCCCCEEECCCCEEEEECC
LEDRFENMGAKLVAEVASKTNEVAGDGTTTATVLAQAMIREGLKNVTAGANPMILRKGID
HHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
KAVTRALEELKAISKPIESKEAIAQVAAISAADEEVGELIAEAMERVGNDGVITIEESRG
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCC
FTTELDVVEGMQFDRGYLSPYMISDSDKMEASLDNPYILITDKKISNIQEIIPVLEQVVQ
CEEEHHHHHCCCCCCCCCCCEEECCCCHHHHCCCCCEEEEECCHHHHHHHHHHHHHHHHH
QGKPILIVAEDIEGDALATLVLNKLRGTFNAVAVKAPGFGDRRKAMLEDLATLTGGQVIT
CCCCEEEEEECCCCHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHCCCCEEH
EDLGLDLKSASLDMLGRASKVVVTKDTTTVVEGAGNEETIKARVQTIRNQIEETTSDFDR
HHHCCCCHHHHHHHHCCCCEEEEECCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHH
EKLQERLAKLAGGVAVVKVGAATETELKERKLRIEDALNATRAAVEEGIVAGGGTSLINV
HHHHHHHHHHHCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHH
IPAVRALLSEVTADEATGVKLVLRALEAPVRQIAENAGEEGSVIVEKLKNESVGVGYNAA
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCCCCCCC
TGEYVDMIAHGIVDPAKVTRSALQNAASVSAMFLTTEAVIADKPEKDAPAMPDMGGMGGM
CHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCC
GGMM
CCCC
>Mature Secondary Structure 
AKDIKFSEDARHAMLRGVDALADAVKVTIGPKGRNVVLEKKFGSPLITNDGVTIAKEIE
CCCCCCCHHHHHHHHHHHHHHHHHHEEEECCCCCEEEEEECCCCCEEECCCCEEEEECC
LEDRFENMGAKLVAEVASKTNEVAGDGTTTATVLAQAMIREGLKNVTAGANPMILRKGID
HHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
KAVTRALEELKAISKPIESKEAIAQVAAISAADEEVGELIAEAMERVGNDGVITIEESRG
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCC
FTTELDVVEGMQFDRGYLSPYMISDSDKMEASLDNPYILITDKKISNIQEIIPVLEQVVQ
CEEEHHHHHCCCCCCCCCCCEEECCCCHHHHCCCCCEEEEECCHHHHHHHHHHHHHHHHH
QGKPILIVAEDIEGDALATLVLNKLRGTFNAVAVKAPGFGDRRKAMLEDLATLTGGQVIT
CCCCEEEEEECCCCHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHCCCCEEH
EDLGLDLKSASLDMLGRASKVVVTKDTTTVVEGAGNEETIKARVQTIRNQIEETTSDFDR
HHHCCCCHHHHHHHHCCCCEEEEECCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHH
EKLQERLAKLAGGVAVVKVGAATETELKERKLRIEDALNATRAAVEEGIVAGGGTSLINV
HHHHHHHHHHHCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHH
IPAVRALLSEVTADEATGVKLVLRALEAPVRQIAENAGEEGSVIVEKLKNESVGVGYNAA
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCCCCCCC
TGEYVDMIAHGIVDPAKVTRSALQNAASVSAMFLTTEAVIADKPEKDAPAMPDMGGMGGM
CHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCC
GGMM
CCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA