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The map label for this gene is rsbW [H]

Identifier: 229917799

GI number: 229917799

Start: 2026746

End: 2027225

Strand: Direct

Name: rsbW [H]

Synonym: EAT1b_2077

Alternate gene names: 229917799

Gene position: 2026746-2027225 (Clockwise)

Preceding gene: 229917798

Following gene: 229917800

Centisome position: 67.56

GC content: 48.12

Gene sequence:

>480_bases
ATGAACAATGTTGAACAGACGGTCATGACGTTCCCGGCCAAACCCGAATATGTCGGTGTGGTTCGATTAGTCGTTTCAGG
AATCGCTAACCGTATGGGATATACGTACGACGAGATCGAGGATATTAAAATCGCGGTCTCGGAAGCATGTGGCAACGCCG
TCCAACATGCTTATGACGATCATCAAGATGGGGAAGTCAAACTCGCTTGTGGCGTCTATGCAGATCGTCTTGAAATCACG
ATTGAGGATTCAGGCAAGACGTTCGCAGATGATGTGAAGCGTCAAGCCGCTCCGGTCGAAGAGACGACCGAAATTGAATC
GCTCCATGAAGGAGGACTTGGTCTCTTCTTGATCGAAGCATTAATGGACGATGTCTCGATCAATAAAGACAACGGTGTCA
AAGTGACGATGACAAAACTCCTCAATAGGGACGAGGTGGATCAGAGTGCCAGCAAAATCTCAACAACGCCATCACAGTGA

Upstream 100 bases:

>100_bases
TGTCACTTGTCGGAGTCACAGACCGAGTCAATCGTCTATTCGAATTGACGGGTCTTCACAAACTTCTTTCTATTAATTCG
AACGTAAGAGGTGGCACGCA

Downstream 100 bases:

>100_bases
TGACGAGGTACTAGAGTGGATTGAACGCTATCAACAAGACGATCAAAACGAAGAAGTGCAAATGCTCCTCATCAACCGCT
ATTCTGACCTCGTTGAAGCA

Product: serine-protein kinase RsbW

Products: NA

Alternate protein names: Anti-sigma-B factor; Sigma-B negative effector rsbW [H]

Number of amino acids: Translated: 159; Mature: 159

Protein sequence:

>159_residues
MNNVEQTVMTFPAKPEYVGVVRLVVSGIANRMGYTYDEIEDIKIAVSEACGNAVQHAYDDHQDGEVKLACGVYADRLEIT
IEDSGKTFADDVKRQAAPVEETTEIESLHEGGLGLFLIEALMDDVSINKDNGVKVTMTKLLNRDEVDQSASKISTTPSQ

Sequences:

>Translated_159_residues
MNNVEQTVMTFPAKPEYVGVVRLVVSGIANRMGYTYDEIEDIKIAVSEACGNAVQHAYDDHQDGEVKLACGVYADRLEIT
IEDSGKTFADDVKRQAAPVEETTEIESLHEGGLGLFLIEALMDDVSINKDNGVKVTMTKLLNRDEVDQSASKISTTPSQ
>Mature_159_residues
MNNVEQTVMTFPAKPEYVGVVRLVVSGIANRMGYTYDEIEDIKIAVSEACGNAVQHAYDDHQDGEVKLACGVYADRLEIT
IEDSGKTFADDVKRQAAPVEETTEIESLHEGGLGLFLIEALMDDVSINKDNGVKVTMTKLLNRDEVDQSASKISTTPSQ

Specific function: Negative regulator of sigma-B activity. Phosphorylates and inactivates its specific antagonist protein, rsbV. Upon phosphorylation of rsbV, rsbW is released and binds to sigma-B, thereby blocking its ability to form an RNA polymerase holoenzyme (E-sigma-B

COG id: COG2172

COG function: function code T; Anti-sigma regulatory factor (Ser/Thr protein kinase)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the anti-sigma-factor family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010193
- InterPro:   IPR003594 [H]

Pfam domain/function: PF02518 HATPase_c [H]

EC number: =2.7.11.1 [H]

Molecular weight: Translated: 17384; Mature: 17384

Theoretical pI: Translated: 4.17; Mature: 4.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNVEQTVMTFPAKPEYVGVVRLVVSGIANRMGYTYDEIEDIKIAVSEACGNAVQHAYDD
CCCHHHHHEECCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCC
HQDGEVKLACGVYADRLEITIEDSGKTFADDVKRQAAPVEETTEIESLHEGGLGLFLIEA
CCCCCEEEEECEEECEEEEEEECCCCHHHHHHHHHCCCCHHHHHHHHHHCCCHHHHHHHH
LMDDVSINKDNGVKVTMTKLLNRDEVDQSASKISTTPSQ
HHHHCCCCCCCCEEEEHHHHHCHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MNNVEQTVMTFPAKPEYVGVVRLVVSGIANRMGYTYDEIEDIKIAVSEACGNAVQHAYDD
CCCHHHHHEECCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCC
HQDGEVKLACGVYADRLEITIEDSGKTFADDVKRQAAPVEETTEIESLHEGGLGLFLIEA
CCCCCEEEEECEEECEEEEEEECCCCHHHHHHHHHCCCCHHHHHHHHHHCCCHHHHHHHH
LMDDVSINKDNGVKVTMTKLLNRDEVDQSASKISTTPSQ
HHHHCCCCCCCCEEEEHHHHHCHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12235376 [H]