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The map label for this gene is sigB [H]

Identifier: 229917800

GI number: 229917800

Start: 2027191

End: 2027991

Strand: Direct

Name: sigB [H]

Synonym: EAT1b_2078

Alternate gene names: 229917800

Gene position: 2027191-2027991 (Clockwise)

Preceding gene: 229917799

Following gene: 229917801

Centisome position: 67.58

GC content: 48.81

Gene sequence:

>801_bases
GTGCCAGCAAAATCTCAACAACGCCATCACAGTGATGACGAGGTACTAGAGTGGATTGAACGCTATCAACAAGACGATCA
AAACGAAGAAGTGCAAATGCTCCTCATCAACCGCTATTCTGACCTCGTTGAAGCACTCGCGCGTAAATTCTCGCGTGGCC
GTGCGATTCATGACGATCTCGTACAAGTTGGGATGATTGGGTTATTGGCGGCACTTCGTCGATTTGACCCGGAATTTGGT
CGTAGCTTCGAATCGTTTGCTGTCCCGACCATCATCGGCGAAATCAAGCGATTCATTCGTGACAAGACGTGGAGTGTGCA
CGTGCCACGTCGAATCAAAGAACTCGGTCCAAAAATCAAGAAGACAGTCGAGGAGTTGACGACCGAATTACAACGTTCGC
CACGAATCGATGAAATTGCGGATCATCTCGGCGTGTCCGATGAAGAAATTCTCGAAACGCTCGAGATGGGTAAGAGTTAT
CAAGCCTTGTCGGTTGATAGTTCAATCGAAGCGGATAACGAAGGTAGTACGGTCACATTACTCGACTTGGTCGGTAGCCA
AGAGCGCGGGTACGAATCGGTCGACCAGCGTCTCATCCTAGAGAAAGCGTTCAATGTCTTAAACGAGCGTGAACGCGCCA
TCTTGGAATGCGCCTACTATAAGAATATGAGTCAAAAAGAGACAGGAGAACTTCTTGGAATCTCGCAGATGCATGTGTCC
CGTTTGCAACGGCGAGCACTCGAAAAGCTGCGTGAAGCCATCAAGGTCCCACTCAATGAAGTATTCTCAAGCGATGACTA
A

Upstream 100 bases:

>100_bases
TTCTTGATCGAAGCATTAATGGACGATGTCTCGATCAATAAAGACAACGGTGTCAAAGTGACGATGACAAAACTCCTCAA
TAGGGACGAGGTGGATCAGA

Downstream 100 bases:

>100_bases
TCGAGTCATCGCTTTTTTGTTTGAATGTTTGCTACACTTTGAAAGAGATCAAAAAAGGAAGTGATTCAAGTGATTACGAA
AGTGGCTAACGCGTTAAACT

Product: RNA polymerase sigma factor SigB

Products: NA

Alternate protein names: General stress protein 84; GSP84; Sigma-37 [H]

Number of amino acids: Translated: 266; Mature: 265

Protein sequence:

>266_residues
MPAKSQQRHHSDDEVLEWIERYQQDDQNEEVQMLLINRYSDLVEALARKFSRGRAIHDDLVQVGMIGLLAALRRFDPEFG
RSFESFAVPTIIGEIKRFIRDKTWSVHVPRRIKELGPKIKKTVEELTTELQRSPRIDEIADHLGVSDEEILETLEMGKSY
QALSVDSSIEADNEGSTVTLLDLVGSQERGYESVDQRLILEKAFNVLNERERAILECAYYKNMSQKETGELLGISQMHVS
RLQRRALEKLREAIKVPLNEVFSSDD

Sequences:

>Translated_266_residues
MPAKSQQRHHSDDEVLEWIERYQQDDQNEEVQMLLINRYSDLVEALARKFSRGRAIHDDLVQVGMIGLLAALRRFDPEFG
RSFESFAVPTIIGEIKRFIRDKTWSVHVPRRIKELGPKIKKTVEELTTELQRSPRIDEIADHLGVSDEEILETLEMGKSY
QALSVDSSIEADNEGSTVTLLDLVGSQERGYESVDQRLILEKAFNVLNERERAILECAYYKNMSQKETGELLGISQMHVS
RLQRRALEKLREAIKVPLNEVFSSDD
>Mature_265_residues
PAKSQQRHHSDDEVLEWIERYQQDDQNEEVQMLLINRYSDLVEALARKFSRGRAIHDDLVQVGMIGLLAALRRFDPEFGR
SFESFAVPTIIGEIKRFIRDKTWSVHVPRRIKELGPKIKKTVEELTTELQRSPRIDEIADHLGVSDEEILETLEMGKSYQ
ALSVDSSIEADNEGSTVTLLDLVGSQERGYESVDQRLILEKAFNVLNERERAILECAYYKNMSQKETGELLGISQMHVSR
LQRRALEKLREAIKVPLNEVFSSDD

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. Sigma B is not essential for sporulation; rather it is required for maximal expression of ctc and csbA which are transcr

COG id: COG1191

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family. SigB subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788231, Length=203, Percent_Identity=30.5418719211823, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1789098, Length=226, Percent_Identity=29.2035398230088, Blast_Score=74, Evalue=1e-14,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR014288
- InterPro:   IPR014322
- InterPro:   IPR000943
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR011991 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 30643; Mature: 30512

Theoretical pI: Translated: 4.88; Mature: 4.88

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPAKSQQRHHSDDEVLEWIERYQQDDQNEEVQMLLINRYSDLVEALARKFSRGRAIHDDL
CCCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
VQVGMIGLLAALRRFDPEFGRSFESFAVPTIIGEIKRFIRDKTWSVHVPRRIKELGPKIK
HHHHHHHHHHHHHHHCHHHCCCHHHHHHHHHHHHHHHHHHCCCCCEECCHHHHHHCHHHH
KTVEELTTELQRSPRIDEIADHLGVSDEEILETLEMGKSYQALSVDSSIEADNEGSTVTL
HHHHHHHHHHHHCCCHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEH
LDLVGSQERGYESVDQRLILEKAFNVLNERERAILECAYYKNMSQKETGELLGISQMHVS
HHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
RLQRRALEKLREAIKVPLNEVFSSDD
HHHHHHHHHHHHHHHCCHHHHCCCCC
>Mature Secondary Structure 
PAKSQQRHHSDDEVLEWIERYQQDDQNEEVQMLLINRYSDLVEALARKFSRGRAIHDDL
CCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
VQVGMIGLLAALRRFDPEFGRSFESFAVPTIIGEIKRFIRDKTWSVHVPRRIKELGPKIK
HHHHHHHHHHHHHHHCHHHCCCHHHHHHHHHHHHHHHHHHCCCCCEECCHHHHHHCHHHH
KTVEELTTELQRSPRIDEIADHLGVSDEEILETLEMGKSYQALSVDSSIEADNEGSTVTL
HHHHHHHHHHHHCCCHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEH
LDLVGSQERGYESVDQRLILEKAFNVLNERERAILECAYYKNMSQKETGELLGISQMHVS
HHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
RLQRRALEKLREAIKVPLNEVFSSDD
HHHHHHHHHHHHHHHCCHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3016731; 3027048; 2170324; 9384377; 9298659 [H]