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The map label for this gene is glnT [H]

Identifier: 229917772

GI number: 229917772

Start: 2003898

End: 2005343

Strand: Direct

Name: glnT [H]

Synonym: EAT1b_2050

Alternate gene names: 229917772

Gene position: 2003898-2005343 (Clockwise)

Preceding gene: 229917771

Following gene: 229917773

Centisome position: 66.8

GC content: 51.11

Gene sequence:

>1446_bases
ATGGAGAAATTGGTAGAGAAACTCGTTTCGCAAGCAAACGATTTGTTGTGGTCGCAAGTGCTCATCGTTGTACTTGTCGC
GTTAGGTCTTTATTTTACGGTTCGTATGGGCTTCGTTCAGTTCCGCATGATTCCTGAAATGTTCCGCCTCTTGTTCAAAG
AAGGCACAGAGCGTGAAAAAGGCCAAGTGTCTTCGTTCCAGGCGTTCGCAATCGGGACGGCAGCACGTGTCGGGACGGGT
AACATTGCCGGTGTGGCGACAGCGATTGCGCTAGGTGGCCCCGGAGCAGTTTTCTGGATGTGGCTCATCGCGTTGATTGG
AGCAGCATCTGCATTCGTCGAAAGTACATTGGCACAAGTGTACAAGATTCGTGATGGACGCACATGGCGTGGGGGACCTG
CCTACTATATGGAGCGTGCGCTCGGTCAACGCTGGCTCGGTGTCTTGTTCGCGATTTTGATCACGTTCTCATTCGGATTT
GCGTTTAACTCGGTACAGTCGAACACGATTTCACTCTCTGTGAGTAACCAGTTCGGGATCAGCACAACAGTCGTCGGGAT
TGTCCTTGCGATTGTGACGGCCATTGTCGTCTTCGGTGGAATCCAATCGATCGCACTCTTGTCGAGTATCATCGTACCGG
TCATGGCAGGTGGCTATATCTTACTCGCGATTTGGATTATGGTGACGAACGTCTCCCTTCTACCAGACGTCTTCAGCCTC
ATCTTCTCAGAAGGAGTCCTCGGAATCGAGCAACTCTTCGGAGGAGCGATTGGCGCCATGGTACTTCAAGGGATTCGCCG
CGGTCTCTTCTCGAACGAAGCGGGTATGGGTTCTGCACCGAACGCCGCTGCGACAGCAGAAGTATCTCACCCTGTCAAAC
AAGGATTGATTCAATCGTTTAGCGTATTCGTAGATACATTGCTCGTTTGTACATCGACAGCGATGATCATCTTGATTTCA
GGCGTAGCCGGTTCAGAGTCGTTCGCAGGTGTCGAATTGACGCAAGCCGCGTTGACAGCAGAAATCGGATCAGTCGCAAC
ATCGTTCTTGACGATTGCAATCTTCTTGTTCGCATTCAGTTCGATTCTTGGAAACTACTACTACGGTGAGACGAACATCC
AGTTCATCTCGGATAAGAAAATCTACGTTACTCTCTATCGCATCGGTGTCGTCGGAATGGTCTTGTTTGGTGCGGTCCGC
TCTGCCGGCCTCGTCTGGTCGATTGCTGACGTGTTCATGGGTCTCATGGCACTCGTGAACTTGTATGCCATCTTCCGTTT
GGCGAAAGTGGCTCGAGCCGTTCTCGATGACTACTTGGCACAGCGTCGCAAAGGAGAGGACCCGGTCTTCTATGTCGACT
CGGTTGAGAACCTCCCTGGTCGTGAATACATCGAAGCTTGGGAAAGCGAAACAGAAGAACAACAAACAGGAACAAGTCGT
TCTTGA

Upstream 100 bases:

>100_bases
AATATTGCAATTTTCGTATAATTTACCTATAATTCTAGTCATGTTAAAAAAGTTGACATGACTTTTTCTGTTGAGATTTT
TAGAATGATGGAGGATTAGT

Downstream 100 bases:

>100_bases
TGAATGGAACCGTCTACGAATTCACGTAGGCGGTTTTTTTACGTATACAAGACGGGTAATGTCATAAAAAAGGGGGCGTT
CATGTATGGAGACGACGCAT

Product: amino acid carrier protein

Products: Na (I) [Cytoplasm]; L-alanine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 481; Mature: 481

Protein sequence:

>481_residues
MEKLVEKLVSQANDLLWSQVLIVVLVALGLYFTVRMGFVQFRMIPEMFRLLFKEGTEREKGQVSSFQAFAIGTAARVGTG
NIAGVATAIALGGPGAVFWMWLIALIGAASAFVESTLAQVYKIRDGRTWRGGPAYYMERALGQRWLGVLFAILITFSFGF
AFNSVQSNTISLSVSNQFGISTTVVGIVLAIVTAIVVFGGIQSIALLSSIIVPVMAGGYILLAIWIMVTNVSLLPDVFSL
IFSEGVLGIEQLFGGAIGAMVLQGIRRGLFSNEAGMGSAPNAAATAEVSHPVKQGLIQSFSVFVDTLLVCTSTAMIILIS
GVAGSESFAGVELTQAALTAEIGSVATSFLTIAIFLFAFSSILGNYYYGETNIQFISDKKIYVTLYRIGVVGMVLFGAVR
SAGLVWSIADVFMGLMALVNLYAIFRLAKVARAVLDDYLAQRRKGEDPVFYVDSVENLPGREYIEAWESETEEQQTGTSR
S

Sequences:

>Translated_481_residues
MEKLVEKLVSQANDLLWSQVLIVVLVALGLYFTVRMGFVQFRMIPEMFRLLFKEGTEREKGQVSSFQAFAIGTAARVGTG
NIAGVATAIALGGPGAVFWMWLIALIGAASAFVESTLAQVYKIRDGRTWRGGPAYYMERALGQRWLGVLFAILITFSFGF
AFNSVQSNTISLSVSNQFGISTTVVGIVLAIVTAIVVFGGIQSIALLSSIIVPVMAGGYILLAIWIMVTNVSLLPDVFSL
IFSEGVLGIEQLFGGAIGAMVLQGIRRGLFSNEAGMGSAPNAAATAEVSHPVKQGLIQSFSVFVDTLLVCTSTAMIILIS
GVAGSESFAGVELTQAALTAEIGSVATSFLTIAIFLFAFSSILGNYYYGETNIQFISDKKIYVTLYRIGVVGMVLFGAVR
SAGLVWSIADVFMGLMALVNLYAIFRLAKVARAVLDDYLAQRRKGEDPVFYVDSVENLPGREYIEAWESETEEQQTGTSR
S
>Mature_481_residues
MEKLVEKLVSQANDLLWSQVLIVVLVALGLYFTVRMGFVQFRMIPEMFRLLFKEGTEREKGQVSSFQAFAIGTAARVGTG
NIAGVATAIALGGPGAVFWMWLIALIGAASAFVESTLAQVYKIRDGRTWRGGPAYYMERALGQRWLGVLFAILITFSFGF
AFNSVQSNTISLSVSNQFGISTTVVGIVLAIVTAIVVFGGIQSIALLSSIIVPVMAGGYILLAIWIMVTNVSLLPDVFSL
IFSEGVLGIEQLFGGAIGAMVLQGIRRGLFSNEAGMGSAPNAAATAEVSHPVKQGLIQSFSVFVDTLLVCTSTAMIILIS
GVAGSESFAGVELTQAALTAEIGSVATSFLTIAIFLFAFSSILGNYYYGETNIQFISDKKIYVTLYRIGVVGMVLFGAVR
SAGLVWSIADVFMGLMALVNLYAIFRLAKVARAVLDDYLAQRRKGEDPVFYVDSVENLPGREYIEAWESETEEQQTGTSR
S

Specific function: Probably functions as a sodium/glutamine symporter for glutamine uptake [H]

COG id: COG1115

COG function: function code E; Na+/alanine symporter

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium:alanine (SAF) symporter family [H]

Homologues:

Organism=Escherichia coli, GI1786188, Length=472, Percent_Identity=41.3135593220339, Blast_Score=329, Evalue=2e-91,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001463 [H]

Pfam domain/function: PF01235 Na_Ala_symp [H]

EC number: NA

Molecular weight: Translated: 51725; Mature: 51725

Theoretical pI: Translated: 5.06; Mature: 5.06

Prosite motif: PS00873 NA_ALANINE_SYMP ; PS00287 CYSTATIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKLVEKLVSQANDLLWSQVLIVVLVALGLYFTVRMGFVQFRMIPEMFRLLFKEGTEREK
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
GQVSSFQAFAIGTAARVGTGNIAGVATAIALGGPGAVFWMWLIALIGAASAFVESTLAQV
CCHHHHHHHHHHCHHHCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
YKIRDGRTWRGGPAYYMERALGQRWLGVLFAILITFSFGFAFNSVQSNTISLSVSNQFGI
HHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCH
STTVVGIVLAIVTAIVVFGGIQSIALLSSIIVPVMAGGYILLAIWIMVTNVSLLPDVFSL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHH
IFSEGVLGIEQLFGGAIGAMVLQGIRRGLFSNEAGMGSAPNAAATAEVSHPVKQGLIQSF
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
SVFVDTLLVCTSTAMIILISGVAGSESFAGVELTQAALTAEIGSVATSFLTIAIFLFAFS
HHHHHHHHHHHHHHHHHEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SILGNYYYGETNIQFISDKKIYVTLYRIGVVGMVLFGAVRSAGLVWSIADVFMGLMALVN
HHHCCCEECCCCEEEEECCEEEEHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
LYAIFRLAKVARAVLDDYLAQRRKGEDPVFYVDSVENLPGREYIEAWESETEEQQTGTSR
HHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCC
S
C
>Mature Secondary Structure
MEKLVEKLVSQANDLLWSQVLIVVLVALGLYFTVRMGFVQFRMIPEMFRLLFKEGTEREK
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
GQVSSFQAFAIGTAARVGTGNIAGVATAIALGGPGAVFWMWLIALIGAASAFVESTLAQV
CCHHHHHHHHHHCHHHCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
YKIRDGRTWRGGPAYYMERALGQRWLGVLFAILITFSFGFAFNSVQSNTISLSVSNQFGI
HHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCH
STTVVGIVLAIVTAIVVFGGIQSIALLSSIIVPVMAGGYILLAIWIMVTNVSLLPDVFSL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHH
IFSEGVLGIEQLFGGAIGAMVLQGIRRGLFSNEAGMGSAPNAAATAEVSHPVKQGLIQSF
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
SVFVDTLLVCTSTAMIILISGVAGSESFAGVELTQAALTAEIGSVATSFLTIAIFLFAFS
HHHHHHHHHHHHHHHHHEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SILGNYYYGETNIQFISDKKIYVTLYRIGVVGMVLFGAVRSAGLVWSIADVFMGLMALVN
HHHCCCEECCCCEEEEECCEEEEHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
LYAIFRLAKVARAVLDDYLAQRRKGEDPVFYVDSVENLPGREYIEAWESETEEQQTGTSR
HHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCC
S
C

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Na (I) [Periplasm]; L-alanine [Periplasm] [C]

Specific reaction: Na (I) [Periplasm] + L-alanine [Periplasm] = Na (I) [Cytoplasm] + L-alanine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]