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The map label for this gene is azoR

Identifier: 229917730

GI number: 229917730

Start: 1959633

End: 1960268

Strand: Direct

Name: azoR

Synonym: EAT1b_2007

Alternate gene names: 229917730

Gene position: 1959633-1960268 (Clockwise)

Preceding gene: 229917729

Following gene: 229917731

Centisome position: 65.32

GC content: 48.43

Gene sequence:

>636_bases
ATGACTAAAGTATTATACGTATCGGCTAACCCGAAACCAACTGAACTTTCATACAGCAAACAAGTGGCAGAAACATTCGT
ATCAACACTAAAGGCTGAAAACGCATCAATCGAAGTGGAAGCAATCGAACTCTACGATGTCGATGTTCAAGAAATCGACG
GAGACGTGCTCAGCGCATGGGGTAAATTCGCATCAGGCGAAGCACTCACTGACGTAGAAGCGAAGAAAGTAGGCACAATG
AGCGGAATGCTTGAGAAATTCATGGAAGCGGACCTTTACGTGTTCGCAACGCCAATGTGGAACTTCTTCTTCCCGGCGCG
CATGAAAATGTTCCTCGACAGCGTCCTGATGGCGGGTAAAACGTTCCGTTACACAGAACAAGGTCCAGTCGGCTTGCTTG
AAAACAAACAAGCGATCCACATCCAAGGAACAGGTGGAATCTACACTGGGACGGACCTCAACTTTGCAGACGCATACCTT
CGCCAAGCACTCGCGTTTGTCGGTGTATCTGAAGTGACGACGGTAGCGGTTGAAGGGATGAACCAATACCCAGACAAAAT
CGAAGAAATCGTTGCGGACGCGAAAGCGAAAGCTGAAGCACTTGCGAAAGAAGTTGCAGGCGCCGTAACGGTATAA

Upstream 100 bases:

>100_bases
GAATATCTTGAAATTAAAATAATTGACGATGAGATATACAGATGATATAGTTCTAACTGTTATTGAAAAATTAAAGATTA
ACCAAATGGAGGATTACACG

Downstream 100 bases:

>100_bases
CCTAGAGAGACGAGTCGACTGACTCGTCTTTTTGTATGCATTCATTTTGGGTATGATACAAAGGGAAAGGGGAGTATGCA
ATGGCAGATCAGTTATCGAG

Product: NAD(P)H dehydrogenase (quinone)

Products: NAD+; NADP+; reduced acceptor

Alternate protein names: Azo-dye reductase; FMN-dependent NADH-azo compound oxidoreductase

Number of amino acids: Translated: 211; Mature: 210

Protein sequence:

>211_residues
MTKVLYVSANPKPTELSYSKQVAETFVSTLKAENASIEVEAIELYDVDVQEIDGDVLSAWGKFASGEALTDVEAKKVGTM
SGMLEKFMEADLYVFATPMWNFFFPARMKMFLDSVLMAGKTFRYTEQGPVGLLENKQAIHIQGTGGIYTGTDLNFADAYL
RQALAFVGVSEVTTVAVEGMNQYPDKIEEIVADAKAKAEALAKEVAGAVTV

Sequences:

>Translated_211_residues
MTKVLYVSANPKPTELSYSKQVAETFVSTLKAENASIEVEAIELYDVDVQEIDGDVLSAWGKFASGEALTDVEAKKVGTM
SGMLEKFMEADLYVFATPMWNFFFPARMKMFLDSVLMAGKTFRYTEQGPVGLLENKQAIHIQGTGGIYTGTDLNFADAYL
RQALAFVGVSEVTTVAVEGMNQYPDKIEEIVADAKAKAEALAKEVAGAVTV
>Mature_210_residues
TKVLYVSANPKPTELSYSKQVAETFVSTLKAENASIEVEAIELYDVDVQEIDGDVLSAWGKFASGEALTDVEAKKVGTMS
GMLEKFMEADLYVFATPMWNFFFPARMKMFLDSVLMAGKTFRYTEQGPVGLLENKQAIHIQGTGGIYTGTDLNFADAYLR
QALAFVGVSEVTTVAVEGMNQYPDKIEEIVADAKAKAEALAKEVAGAVTV

Specific function: Catalyzes the reductive cleavage of azo bond in aromatic azo compounds to the corresponding amines. Requires NADH, but not NADPH, as an electron donor for its activity

COG id: COG1182

COG function: function code I; Acyl carrier protein phosphodiesterase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the azoreductase type 1 family

Homologues:

Organism=Escherichia coli, GI1787680, Length=185, Percent_Identity=30.2702702702703, Blast_Score=93, Evalue=1e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): AZOR_EXISA (C4L0W8)

Other databases:

- EMBL:   CP001615
- RefSeq:   YP_002886376.1
- ProteinModelPortal:   C4L0W8
- GeneID:   7868814
- GenomeReviews:   CP001615_GR
- KEGG:   eat:EAT1b_2007
- OMA:   FSGWGKL
- ProtClustDB:   CLSK2489999
- HAMAP:   MF_01216
- InterPro:   IPR003680
- InterPro:   IPR023048

Pfam domain/function: PF02525 Flavodoxin_2

EC number: 1.6.99.2

Molecular weight: Translated: 22966; Mature: 22835

Theoretical pI: Translated: 4.28; Mature: 4.28

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKVLYVSANPKPTELSYSKQVAETFVSTLKAENASIEVEAIELYDVDVQEIDGDVLSAW
CCEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEEEEEECCHHHHCCHHHHHH
GKFASGEALTDVEAKKVGTMSGMLEKFMEADLYVFATPMWNFFFPARMKMFLDSVLMAGK
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCEEEEECCHHHHHHHHHHHHHHHHHHHCCC
TFRYTEQGPVGLLENKQAIHIQGTGGIYTGTDLNFADAYLRQALAFVGVSEVTTVAVEGM
CEEECCCCCCCEECCCCEEEEEECCCEEECCCCCHHHHHHHHHHHHHCHHHHHHHHHHCC
NQYPDKIEEIVADAKAKAEALAKEVAGAVTV
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
TKVLYVSANPKPTELSYSKQVAETFVSTLKAENASIEVEAIELYDVDVQEIDGDVLSAW
CEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEEEEEECCHHHHCCHHHHHH
GKFASGEALTDVEAKKVGTMSGMLEKFMEADLYVFATPMWNFFFPARMKMFLDSVLMAGK
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCEEEEECCHHHHHHHHHHHHHHHHHHHCCC
TFRYTEQGPVGLLENKQAIHIQGTGGIYTGTDLNFADAYLRQALAFVGVSEVTTVAVEGM
CEEECCCCCCCEECCCCEEEEEECCCEEECCCCCHHHHHHHHHHHHHCHHHHHHHHHHCC
NQYPDKIEEIVADAKAKAEALAKEVAGAVTV
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADH; NADPH; H+; acceptor

Specific reaction: NAD(P)H + H+ + acceptor = NAD(P)+ + reduced acceptor

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA