Click here to switch to the map view.

The map label for this gene is yfkO [H]

Identifier: 229917616

GI number: 229917616

Start: 1857046

End: 1857708

Strand: Direct

Name: yfkO [H]

Synonym: EAT1b_1891

Alternate gene names: 229917616

Gene position: 1857046-1857708 (Clockwise)

Preceding gene: 229917615

Following gene: 229917619

Centisome position: 61.9

GC content: 49.02

Gene sequence:

>663_bases
ATGAAAAAGAAAGACATTTTAGAGGCTTATCGTTGGCGTCAAGCGACGAAAGCGTTCGATGCGGATCGTAAAATCAAAGA
AGAGGACATGGAGTTCATTTTAGAGACGGGACGTCTTTCGCCGAGTTCGTTCGGATTCGAGCCATGGCAATTCGTCGTGG
TGCGTCGGGAAGATTTACTCCAAAAGATTAAAGATCATGCATGGGGAGCGCAAGGTCAGGCACTGACTGCCAGTCACTTC
GTGTTGATCCTCGCAAGAACACCGAAAGCCATGCGTTACGATGCAGACTATATTCGTCACATCGTCACAGGCGTGCAAGG
GCGGACGGAAGAGGAGTACGCACAACGCGTGAAACGGGTACAGGAGTTCCAGGAGAACGACTATCGTCTCTTAGACGATG
AGCGTGTCTTCCAGGAATGGATCAAGCGTCAGACATATATCCCTCTTGGGAACATGATGACGGCTGCCGCACAAATCGAA
ATTGATTCATGCCCGATTGAAGGGTTCAATCAAGAGGAGATTGATGAGTTGTTAATCTCAGAAGGTGTGATGGAACGTGG
GGAATGGTCACTTTCGGTCATGGTCGCATTTGGGTATCGAGCGCGCGATTTCGCACCGAAAACACGTCGTGACTTTAACG
AGGTCGTCAAATATATCGGATGA

Upstream 100 bases:

>100_bases
ATACAAACGGTGCGATTGCCGCTGAAGAATCTTCATGAGCGATTTTTGATTTACCGGTTTGATATGATGGAATCATCTTG
GTGATGGAGGAGTGAGCACG

Downstream 100 bases:

>100_bases
AAAAAAGAGTCAATCGCCGGGGCGATTGACTCTTTTGAATTATTTCCAGTCTGTGATGCGGTCGACTGGGAAGCGGACTG
ATGGATACCCTTCTTCTGCT

Product: nitroreductase

Products: NAD(P)(+); @TETR01.txt*5,6,7,8-Tetrahydropteridine!. [C]

Alternate protein names: NA

Number of amino acids: Translated: 220; Mature: 220

Protein sequence:

>220_residues
MKKKDILEAYRWRQATKAFDADRKIKEEDMEFILETGRLSPSSFGFEPWQFVVVRREDLLQKIKDHAWGAQGQALTASHF
VLILARTPKAMRYDADYIRHIVTGVQGRTEEEYAQRVKRVQEFQENDYRLLDDERVFQEWIKRQTYIPLGNMMTAAAQIE
IDSCPIEGFNQEEIDELLISEGVMERGEWSLSVMVAFGYRARDFAPKTRRDFNEVVKYIG

Sequences:

>Translated_220_residues
MKKKDILEAYRWRQATKAFDADRKIKEEDMEFILETGRLSPSSFGFEPWQFVVVRREDLLQKIKDHAWGAQGQALTASHF
VLILARTPKAMRYDADYIRHIVTGVQGRTEEEYAQRVKRVQEFQENDYRLLDDERVFQEWIKRQTYIPLGNMMTAAAQIE
IDSCPIEGFNQEEIDELLISEGVMERGEWSLSVMVAFGYRARDFAPKTRRDFNEVVKYIG
>Mature_220_residues
MKKKDILEAYRWRQATKAFDADRKIKEEDMEFILETGRLSPSSFGFEPWQFVVVRREDLLQKIKDHAWGAQGQALTASHF
VLILARTPKAMRYDADYIRHIVTGVQGRTEEEYAQRVKRVQEFQENDYRLLDDERVFQEWIKRQTYIPLGNMMTAAAQIE
IDSCPIEGFNQEEIDELLISEGVMERGEWSLSVMVAFGYRARDFAPKTRRDFNEVVKYIG

Specific function: Reduction Of A Variety Of Nitroaromatic Compounds Using NADH (And To Lesser Extent Nadph) As Source Of Reducing Equivalents; Two Electrons Are Transferred. Capable Of Reducing Nitrofurazone, Quinones And The Anti-Tumor Agent Cb1954 (5- (Aziridin-1-Yl)-2,4

COG id: COG0778

COG function: function code C; Nitroreductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nitroreductase family [H]

Homologues:

Organism=Escherichia coli, GI1786792, Length=216, Percent_Identity=31.9444444444444, Blast_Score=86, Evalue=2e-18,

Paralogues:

None

Copy number: 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000415 [H]

Pfam domain/function: PF00881 Nitroreductase [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 25885; Mature: 25885

Theoretical pI: Translated: 5.25; Mature: 5.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKKDILEAYRWRQATKAFDADRKIKEEDMEFILETGRLSPSSFGFEPWQFVVVRREDLL
CCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHEEEHHHHHH
QKIKDHAWGAQGQALTASHFVLILARTPKAMRYDADYIRHIVTGVQGRTEEEYAQRVKRV
HHHHHHCCCCCCCEEEHHHEEEEEECCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
QEFQENDYRLLDDERVFQEWIKRQTYIPLGNMMTAAAQIEIDSCPIEGFNQEEIDELLIS
HHHHCCCCEECCHHHHHHHHHHHCCCCCCCHHHHHHHEEEECCCCCCCCCHHHHHHHHHH
EGVMERGEWSLSVMVAFGYRARDFAPKTRRDFNEVVKYIG
HHHHHCCCCCEEEEEEECCHHHCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MKKKDILEAYRWRQATKAFDADRKIKEEDMEFILETGRLSPSSFGFEPWQFVVVRREDLL
CCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHEEEHHHHHH
QKIKDHAWGAQGQALTASHFVLILARTPKAMRYDADYIRHIVTGVQGRTEEEYAQRVKRV
HHHHHHCCCCCCCEEEHHHEEEEEECCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
QEFQENDYRLLDDERVFQEWIKRQTYIPLGNMMTAAAQIEIDSCPIEGFNQEEIDELLIS
HHHHCCCCEECCHHHHHHHHHHHCCCCCCCHHHHHHHEEEECCCCCCCCCHHHHHHHHHH
EGVMERGEWSLSVMVAFGYRARDFAPKTRRDFNEVVKYIG
HHHHHCCCCCEEEEEEECCHHHCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: FMN. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NAD(P)H; 6,7-Dihydropteridine [C]

Specific reaction: NAD(P)H + 6,7-Dihydropteridine = NAD(P)(+) + @TETR01.txt*5,6,7,8-Tetrahydropteridine!. [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]