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The map label for this gene is nsr [H]

Identifier: 229917586

GI number: 229917586

Start: 1828588

End: 1829262

Strand: Direct

Name: nsr [H]

Synonym: EAT1b_1861

Alternate gene names: 229917586

Gene position: 1828588-1829262 (Clockwise)

Preceding gene: 229917585

Following gene: 229917587

Centisome position: 60.96

GC content: 47.11

Gene sequence:

>675_bases
ATGATGAATATTATGGATGCACTGACACGATTGCAGTGGAATCATCTGTTTTCGAATGAAACGCTTGAGTCCTCATCCGT
GGTGACGTATGCGTCCGGGACCATGTTATGTTCGAACGATCAACACATCGAATCTTTATTCGTCGTCCTTGAAGGGCGCG
TCAAAATTTATACGCTCAGTGAAGAAGGAAAATTGCGATTGTTGCGTCTGAAGGCTGCTCCAACATTGATTGGAGATATC
GAGTATGCGAGCGGACGCAATGTATTGCATACGGTTGAAGCGGTCGGTCCCGTAACGTGTCTGCGTATCCCTTTCGAGGC
GCTTCGACGATATAATCGCACGGTCGAATTCACGGAGCATCTGTTACGAGGCGTGTCCGAAAAATTATTCATTGAGGCAA
ACGCCTCGTCGAATCATCTCATGTCGACTCTCGAAGAGCGGTTGGCCGGTTACTTGATCTCACTCAGTGAATCTGGGAAT
GAACTGTTTCAAGCGGAGATGAGTCAACTGAAACGGAAAGAAGTCGCAGAATGGCTCGGGACGAGTTATCGTCACTTGAA
CCGAACGCTACAGGCATTCGTGGATGAAGGATTGGTCAAACGAACGAGAAAAGAAGTGATCATCTTAAACAGTGAGCGAT
TGAAGGAACGTGCGAACGGCATGTTTTATGAGTAA

Upstream 100 bases:

>100_bases
GTGTCAAACGGAAACCGATGAACACGGGACAGGTCATGGGACTCGTCTTGATGGTCGTCGGCATCGGAATCTATACATTT
TCTTAAGGAGTTCATTTTAC

Downstream 100 bases:

>100_bases
GGAGGGATTATATGATTTTTGGTATCATCTGTTCGGCAGTGGCTGGGGCATTTATTAGCGTACAGGCGGCGGTCAACGCG
AAAATGAATACAAATTTAGG

Product: Crp/Fnr family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 224; Mature: 224

Protein sequence:

>224_residues
MMNIMDALTRLQWNHLFSNETLESSSVVTYASGTMLCSNDQHIESLFVVLEGRVKIYTLSEEGKLRLLRLKAAPTLIGDI
EYASGRNVLHTVEAVGPVTCLRIPFEALRRYNRTVEFTEHLLRGVSEKLFIEANASSNHLMSTLEERLAGYLISLSESGN
ELFQAEMSQLKRKEVAEWLGTSYRHLNRTLQAFVDEGLVKRTRKEVIILNSERLKERANGMFYE

Sequences:

>Translated_224_residues
MMNIMDALTRLQWNHLFSNETLESSSVVTYASGTMLCSNDQHIESLFVVLEGRVKIYTLSEEGKLRLLRLKAAPTLIGDI
EYASGRNVLHTVEAVGPVTCLRIPFEALRRYNRTVEFTEHLLRGVSEKLFIEANASSNHLMSTLEERLAGYLISLSESGN
ELFQAEMSQLKRKEVAEWLGTSYRHLNRTLQAFVDEGLVKRTRKEVIILNSERLKERANGMFYE
>Mature_224_residues
MMNIMDALTRLQWNHLFSNETLESSSVVTYASGTMLCSNDQHIESLFVVLEGRVKIYTLSEEGKLRLLRLKAAPTLIGDI
EYASGRNVLHTVEAVGPVTCLRIPFEALRRYNRTVEFTEHLLRGVSEKLFIEANASSNHLMSTLEERLAGYLISLSESGN
ELFQAEMSQLKRKEVAEWLGTSYRHLNRTLQAFVDEGLVKRTRKEVIILNSERLKERANGMFYE

Specific function: Transcription regulator involved in mid-term, stationary-phase viability under nitrogen starvation. Might control expression of the salvage pathways or in some other way repress the recycling of nucleobases to nucleic acids and enhance their use as genera

COG id: COG0664

COG function: function code T; cAMP-binding proteins - catabolite gene activator and regulatory subunit of cAMP-dependent protein kinases

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH crp-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788487, Length=176, Percent_Identity=29.5454545454545, Blast_Score=73, Evalue=2e-14,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018490
- InterPro:   IPR000595
- InterPro:   IPR012318
- InterPro:   IPR014710 [H]

Pfam domain/function: PF00027 cNMP_binding [H]

EC number: NA

Molecular weight: Translated: 25602; Mature: 25602

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: PS50042 CNMP_BINDING_3 ; PS51063 HTH_CRP_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMNIMDALTRLQWNHLFSNETLESSSVVTYASGTMLCSNDQHIESLFVVLEGRVKIYTLS
CCCHHHHHHHHHHHHHHCCCCCCCCCEEEEECCEEEECCCHHHHHHHHHEECCEEEEEEC
EEGKLRLLRLKAAPTLIGDIEYASGRNVLHTVEAVGPVTCLRIPFEALRRYNRTVEFTEH
CCCCEEEEEEECCCCEEECCCCCCCCCHHHHHHHHCCHHHHCCCHHHHHHHCCCHHHHHH
LLRGVSEKLFIEANASSNHLMSTLEERLAGYLISLSESGNELFQAEMSQLKRKEVAEWLG
HHHCCCCEEEEEECCCCHHHHHHHHHHHHHHEEEECCCCHHHHHHHHHHHHHHHHHHHHH
TSYRHLNRTLQAFVDEGLVKRTRKEVIILNSERLKERANGMFYE
HHHHHHHHHHHHHHHHHHHHHHHHCEEEECHHHHHHHHCCCCCC
>Mature Secondary Structure
MMNIMDALTRLQWNHLFSNETLESSSVVTYASGTMLCSNDQHIESLFVVLEGRVKIYTLS
CCCHHHHHHHHHHHHHHCCCCCCCCCEEEEECCEEEECCCHHHHHHHHHEECCEEEEEEC
EEGKLRLLRLKAAPTLIGDIEYASGRNVLHTVEAVGPVTCLRIPFEALRRYNRTVEFTEH
CCCCEEEEEEECCCCEEECCCCCCCCCHHHHHHHHCCHHHHCCCHHHHHHHCCCHHHHHH
LLRGVSEKLFIEANASSNHLMSTLEERLAGYLISLSESGNELFQAEMSQLKRKEVAEWLG
HHHCCCCEEEEEECCCCHHHHHHHHHHHHHHEEEECCCCHHHHHHHHHHHHHHHHHHHHH
TSYRHLNRTLQAFVDEGLVKRTRKEVIILNSERLKERANGMFYE
HHHHHHHHHHHHHHHHHHHHHHHHCEEEECHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]