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The map label for this gene is adaA [H]

Identifier: 229917511

GI number: 229917511

Start: 1754428

End: 1754976

Strand: Direct

Name: adaA [H]

Synonym: EAT1b_1786

Alternate gene names: 229917511

Gene position: 1754428-1754976 (Clockwise)

Preceding gene: 229917509

Following gene: 229917512

Centisome position: 58.48

GC content: 49.36

Gene sequence:

>549_bases
ATGTTGACGTTTGACGAAAAGTGGGCGAAAGTGCTCGCCTGCGATGCAACTTATGACGGATTGTTCTATACAGCCGTAAA
AACGACAGGGATCTATTGCCGACCATCGTGCCGATCCCGCAAACCGAAGAAAGAGAACGTTGATTTTTACGTAACGAGAG
AAGAAGCAGAGACGAATGGATTTCGACCGTGCAAGCGATGTCAGCCAGAAGTCGAACACGCCCCACACCGTGCGCTCGTT
CAACAAGTGACGGCGTATCTCGTGAACCATTATAAACAACCCATTCGCCTTGAACAGATTGCCGAACACGCCCAACTGAG
TCCCTTTCATTTAGAGCGGACATTCACCCAGGCGACAGGAGAGACTCCGAGGCAACTACTTGAACGGATTCGAATCGACA
AGGCGGCTTATTTATTGCGAACGACCGACATGAGTAATCTGACGGTCTGCTATGAGGTCGGTTTTCAAACACCATCGAAC
TTTTACCGTGTGTTCCGACGAGTAAAAGGCTGTACACCGAACACGTATCGAAAAGGAGAGGACCTATGA

Upstream 100 bases:

>100_bases
ATCTGTAAAACGTTTCAAAAAGAGGGGATTGTAAGCGCAAGAAAGAGGACGAAATGTTGATTTTAGATTTGCTACAGTGA
GAGAAGAAAGGGGTGTGGAG

Downstream 100 bases:

>100_bases
CAGAGATGCTACTCGCTGTTCAGCAGCCGTTTGATTTTCAAGAGTGTCTCGTCTTTTTAAGTCGGTCAGAACAAGAAGTG
CTCCATGTGACCACACCTGA

Product: AraC family transcriptional regulator

Products: DNA; protein S-methyl-L-cysteine

Alternate protein names: NA

Number of amino acids: Translated: 182; Mature: 182

Protein sequence:

>182_residues
MLTFDEKWAKVLACDATYDGLFYTAVKTTGIYCRPSCRSRKPKKENVDFYVTREEAETNGFRPCKRCQPEVEHAPHRALV
QQVTAYLVNHYKQPIRLEQIAEHAQLSPFHLERTFTQATGETPRQLLERIRIDKAAYLLRTTDMSNLTVCYEVGFQTPSN
FYRVFRRVKGCTPNTYRKGEDL

Sequences:

>Translated_182_residues
MLTFDEKWAKVLACDATYDGLFYTAVKTTGIYCRPSCRSRKPKKENVDFYVTREEAETNGFRPCKRCQPEVEHAPHRALV
QQVTAYLVNHYKQPIRLEQIAEHAQLSPFHLERTFTQATGETPRQLLERIRIDKAAYLLRTTDMSNLTVCYEVGFQTPSN
FYRVFRRVKGCTPNTYRKGEDL
>Mature_182_residues
MLTFDEKWAKVLACDATYDGLFYTAVKTTGIYCRPSCRSRKPKKENVDFYVTREEAETNGFRPCKRCQPEVEHAPHRALV
QQVTAYLVNHYKQPIRLEQIAEHAQLSPFHLERTFTQATGETPRQLLERIRIDKAAYLLRTTDMSNLTVCYEVGFQTPSN
FYRVFRRVKGCTPNTYRKGEDL

Specific function: Methylphosphotriester-DNA alkyltransferase is one of two genes required for the adaptive response of bacteria to alkylating agents. It accepts a methyl group from methylphospho-triesters and then acts as a transcriptional activator of the ada operon [H]

COG id: COG2169

COG function: function code F; Adenosine deaminase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH araC/xylS-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788542, Length=178, Percent_Identity=41.0112359550562, Blast_Score=132, Evalue=2e-32,
Organism=Escherichia coli, GI1790339, Length=99, Percent_Identity=33.3333333333333, Blast_Score=65, Evalue=3e-12,
Organism=Escherichia coli, GI1790497, Length=102, Percent_Identity=30.3921568627451, Blast_Score=61, Evalue=5e-11,
Organism=Escherichia coli, GI1790559, Length=99, Percent_Identity=30.3030303030303, Blast_Score=60, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004026
- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR018062
- InterPro:   IPR018060
- InterPro:   IPR016220 [H]

Pfam domain/function: PF02805 Ada_Zn_binding; PF00165 HTH_AraC [H]

EC number: 2.1.1.63

Molecular weight: Translated: 21236; Mature: 21236

Theoretical pI: Translated: 9.01; Mature: 9.01

Prosite motif: PS01124 HTH_ARAC_FAMILY_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.8 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
3.8 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTFDEKWAKVLACDATYDGLFYTAVKTTGIYCRPSCRSRKPKKENVDFYVTREEAETNG
CCCCHHHHHHHEECCCCCCCEEEEEECCCCEEECCCCCCCCCCCCCCCEEEECCCCCCCC
FRPCKRCQPEVEHAPHRALVQQVTAYLVNHYKQPIRLEQIAEHAQLSPFHLERTFTQATG
CCCHHHCCCCHHHCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCHHHHHHHHHHCC
ETPRQLLERIRIDKAAYLLRTTDMSNLTVCYEVGFQTPSNFYRVFRRVKGCTPNTYRKGE
CCHHHHHHHHHHHHHHHHHEECCCCCEEEEEEECCCCCHHHHHHHHHHHCCCCCCCCCCC
DL
CC
>Mature Secondary Structure
MLTFDEKWAKVLACDATYDGLFYTAVKTTGIYCRPSCRSRKPKKENVDFYVTREEAETNG
CCCCHHHHHHHEECCCCCCCEEEEEECCCCEEECCCCCCCCCCCCCCCEEEECCCCCCCC
FRPCKRCQPEVEHAPHRALVQQVTAYLVNHYKQPIRLEQIAEHAQLSPFHLERTFTQATG
CCCHHHCCCCHHHCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCHHHHHHHHHHCC
ETPRQLLERIRIDKAAYLLRTTDMSNLTVCYEVGFQTPSNFYRVFRRVKGCTPNTYRKGE
CCHHHHHHHHHHHHHHHHHEECCCCCEEEEEEECCCCCHHHHHHHHHHHCCCCCCCCCCC
DL
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA containing 6-O-methylguanine; protein L-cysteine

Specific reaction: DNA (containing 6-O-methylguanine) + protein L-cysteine = DNA (without 6-O-methylguanine) + protein S-methyl-L-cysteine

General reaction: Methyl group transfer [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2120677; 9384377; 1744039 [H]