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The map label for this gene is alkA [H]

Identifier: 229917512

GI number: 229917512

Start: 1754973

End: 1755866

Strand: Direct

Name: alkA [H]

Synonym: EAT1b_1787

Alternate gene names: 229917512

Gene position: 1754973-1755866 (Clockwise)

Preceding gene: 229917511

Following gene: 229917513

Centisome position: 58.5

GC content: 47.65

Gene sequence:

>894_bases
ATGACAGAGATGCTACTCGCTGTTCAGCAGCCGTTTGATTTTCAAGAGTGTCTCGTCTTTTTAAGTCGGTCAGAACAAGA
AGTGCTCCATGTGACCACACCTGACATGGTTCGAAAGTTGATGCGAATCGGGGAACGGCTCATCCTGATCGAGCTTCGAG
AAGAGGTGAATCATATTCATGTACGGTTCCCTTTCGATGAAGTATCAGAGACAGAAAAGGAACATGTCGCACGTGAGGTA
CGAAACTGGTTAGACCTTGAGCGCGATTTAAAACCGTTTGAAACGATGGGGGCGAAAGATGAATTGCTGGCACCGTTGAT
TGAAACACATCGCGGGTTACGGATGATTGGCTTCCCAGATTTGTTCGAGGCCCTCACATGGGCCATCATCGGACAACAAA
TCACCTTGTCGTTTGCGTATACGATCAAACGACGCTTTGTTGAACGATATGGAGACCATCGAGTCATCGAAGGAAGAGCA
TACTGGACGTTCCCGCGGGCGGAACGAATCGCCTTGCTCGAACCGGAAGAATTACGTGAGCTTCAATTCAGTCGCAGGAA
AGCGGAGTATGTCATTGATATTGCACGGGAAATAACAAATGGAGACCTGTCAAAAACGGCTTTACAATCACACTCTTCAG
CTGATATCCGTCAACGGTTACTCGCGATACGAGGTGTCGGAGCATGGACAGCAGACTATGTGTTGATGAAGTGTTTTCAG
GACGCATCAGCCTTTCCGATTGCGGATGTCGGATTGCATCAAGCGATTCAGCATCAGTTAGGGACAGCGAAGAAACCAAC
GATTGAAGAAGTGAAGCGCTACGGTGAATCGTGGCAAGGTTTTGAAGGATACGCGACATTCTATCTGTGGAGGTCATTAT
ATGGGAACGTATAA

Upstream 100 bases:

>100_bases
TCTGCTATGAGGTCGGTTTTCAAACACCATCGAACTTTTACCGTGTGTTCCGACGAGTAAAAGGCTGTACACCGAACACG
TATCGAAAAGGAGAGGACCT

Downstream 100 bases:

>100_bases
ATTAGACGTGGAGACACCGATTGGCGTCCTAGAAATCATCGGGACGGAGCAGGTCGTTCAATCGATCTTATTCGTCGAAC
GGGAAAGCAAACGAAGTATG

Product: DNA-3-methyladenine glycosylase II

Products: NA

Alternate protein names: 3-methyladenine-DNA glycosidase [H]

Number of amino acids: Translated: 297; Mature: 296

Protein sequence:

>297_residues
MTEMLLAVQQPFDFQECLVFLSRSEQEVLHVTTPDMVRKLMRIGERLILIELREEVNHIHVRFPFDEVSETEKEHVAREV
RNWLDLERDLKPFETMGAKDELLAPLIETHRGLRMIGFPDLFEALTWAIIGQQITLSFAYTIKRRFVERYGDHRVIEGRA
YWTFPRAERIALLEPEELRELQFSRRKAEYVIDIAREITNGDLSKTALQSHSSADIRQRLLAIRGVGAWTADYVLMKCFQ
DASAFPIADVGLHQAIQHQLGTAKKPTIEEVKRYGESWQGFEGYATFYLWRSLYGNV

Sequences:

>Translated_297_residues
MTEMLLAVQQPFDFQECLVFLSRSEQEVLHVTTPDMVRKLMRIGERLILIELREEVNHIHVRFPFDEVSETEKEHVAREV
RNWLDLERDLKPFETMGAKDELLAPLIETHRGLRMIGFPDLFEALTWAIIGQQITLSFAYTIKRRFVERYGDHRVIEGRA
YWTFPRAERIALLEPEELRELQFSRRKAEYVIDIAREITNGDLSKTALQSHSSADIRQRLLAIRGVGAWTADYVLMKCFQ
DASAFPIADVGLHQAIQHQLGTAKKPTIEEVKRYGESWQGFEGYATFYLWRSLYGNV
>Mature_296_residues
TEMLLAVQQPFDFQECLVFLSRSEQEVLHVTTPDMVRKLMRIGERLILIELREEVNHIHVRFPFDEVSETEKEHVAREVR
NWLDLERDLKPFETMGAKDELLAPLIETHRGLRMIGFPDLFEALTWAIIGQQITLSFAYTIKRRFVERYGDHRVIEGRAY
WTFPRAERIALLEPEELRELQFSRRKAEYVIDIAREITNGDLSKTALQSHSSADIRQRLLAIRGVGAWTADYVLMKCFQD
ASAFPIADVGLHQAIQHQLGTAKKPTIEEVKRYGESWQGFEGYATFYLWRSLYGNV

Specific function: Hydrolysis of the deoxyribose N-glycosidic bond to excise 3-methyladenine, 3-methylguanine, 7-methylguanine, O2- methylthymine, and O2-methylcytosine from the damaged DNA polymer formed by alkylation lesions [H]

COG id: COG0122

COG function: function code L; 3-methyladenine DNA glycosylase/8-oxoguanine DNA glycosylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alkylbase DNA glycosidase alkA family [H]

Homologues:

Organism=Escherichia coli, GI1788383, Length=287, Percent_Identity=25.0871080139373, Blast_Score=83, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010316
- InterPro:   IPR000035
- InterPro:   IPR011257
- InterPro:   IPR003265
- InterPro:   IPR023170
- InterPro:   IPR012904 [H]

Pfam domain/function: PF00730 HhH-GPD; PF07934 OGG_N [H]

EC number: =3.2.2.21 [H]

Molecular weight: Translated: 34659; Mature: 34528

Theoretical pI: Translated: 6.07; Mature: 6.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEMLLAVQQPFDFQECLVFLSRSEQEVLHVTTPDMVRKLMRIGERLILIELREEVNHIH
CCHHHHHHHCCCCHHHHHHHHHCCCCCEEEECCHHHHHHHHHHCCCEEEHEEHHHHCEEE
VRFPFDEVSETEKEHVAREVRNWLDLERDLKPFETMGAKDELLAPLIETHRGLRMIGFPD
EECCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCHHHHHHHHHHHCCCCEECCCHH
LFEALTWAIIGQQITLSFAYTIKRRFVERYGDHRVIEGRAYWTFPRAERIALLEPEELRE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCEEEECCCCCCEEECCHHHHHH
LQFSRRKAEYVIDIAREITNGDLSKTALQSHSSADIRQRLLAIRGVGAWTADYVLMKCFQ
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHH
DASAFPIADVGLHQAIQHQLGTAKKPTIEEVKRYGESWQGFEGYATFYLWRSLYGNV
CCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TEMLLAVQQPFDFQECLVFLSRSEQEVLHVTTPDMVRKLMRIGERLILIELREEVNHIH
CHHHHHHHCCCCHHHHHHHHHCCCCCEEEECCHHHHHHHHHHCCCEEEHEEHHHHCEEE
VRFPFDEVSETEKEHVAREVRNWLDLERDLKPFETMGAKDELLAPLIETHRGLRMIGFPD
EECCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCHHHHHHHHHHHCCCCEECCCHH
LFEALTWAIIGQQITLSFAYTIKRRFVERYGDHRVIEGRAYWTFPRAERIALLEPEELRE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCEEEECCCCCCEEECCHHHHHH
LQFSRRKAEYVIDIAREITNGDLSKTALQSHSSADIRQRLLAIRGVGAWTADYVLMKCFQ
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHH
DASAFPIADVGLHQAIQHQLGTAKKPTIEEVKRYGESWQGFEGYATFYLWRSLYGNV
CCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8376346; 9384377 [H]