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The map label for this gene is sigV [H]

Identifier: 229917493

GI number: 229917493

Start: 1735123

End: 1735644

Strand: Direct

Name: sigV [H]

Synonym: EAT1b_1768

Alternate gene names: 229917493

Gene position: 1735123-1735644 (Clockwise)

Preceding gene: 229917492

Following gene: 229917494

Centisome position: 57.84

GC content: 43.49

Gene sequence:

>522_bases
ATGAAACGTCTCATTCAGAAAGCGCAACGAGGTAATGATCAAGCTTTCTTAAAGATCTTTCAGCATTACGAGGCCGACCT
ATATCGGATGGCCTATGTATACGTCAAAAATCCGGATGATGCGCTCGACGTCGTGCAAGAAGTCGCTTATCAAGCATTTA
AAAGGATTGGAACGTTACGGGAACCACAATTTTTGAAGACATGGCTGATGAAGATTGCCATCAACTGTGCGCTCGATGTC
ATGAAGAAGAGGAAAAATGTGATTCATTTGATGCCGGAGTTTGAAGAGTCAATTGGAAGTACGGAAACGGATTACGCGAT
AACGATGACACTCGCTGACATGATGGATTCCCTTCATGAGGATGAGCGCAGCGTGGTCGTGTTGAAATATTATCAAGACT
ACACGTTTCAACAAATTTCAGATTTATTGGAATGGCCGATTGGGACGGTGAAGACAACCTTATATCGAGCTGTCGATAAA
TTGAGACGAGAATGGAAGGAGGTCGATTGCTATGGATCGTGA

Upstream 100 bases:

>100_bases
CTGAGATGCTTTTTTTCATTTTTTCCCAAATGAGTAAAGCTAAATGGACGCGTTTCATGTCTAATGAGTGTAAGGGAAAA
AACGAAAAGGGGGACGTTCG

Downstream 100 bases:

>100_bases
GATGAAACGGATGTTGGCGGACATCGAAATTCCAAGTGAACTGCGTGAACGCACCCGACAAGGTGTGAAGCGAGCGAAAC
AAGAGATGCGCCGGGAACCA

Product: RNA polymerase, sigma-24 subunit, ECF subfamily

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 173; Mature: 173

Protein sequence:

>173_residues
MKRLIQKAQRGNDQAFLKIFQHYEADLYRMAYVYVKNPDDALDVVQEVAYQAFKRIGTLREPQFLKTWLMKIAINCALDV
MKKRKNVIHLMPEFEESIGSTETDYAITMTLADMMDSLHEDERSVVVLKYYQDYTFQQISDLLEWPIGTVKTTLYRAVDK
LRREWKEVDCYGS

Sequences:

>Translated_173_residues
MKRLIQKAQRGNDQAFLKIFQHYEADLYRMAYVYVKNPDDALDVVQEVAYQAFKRIGTLREPQFLKTWLMKIAINCALDV
MKKRKNVIHLMPEFEESIGSTETDYAITMTLADMMDSLHEDERSVVVLKYYQDYTFQQISDLLEWPIGTVKTTLYRAVDK
LRREWKEVDCYGS
>Mature_173_residues
MKRLIQKAQRGNDQAFLKIFQHYEADLYRMAYVYVKNPDDALDVVQEVAYQAFKRIGTLREPQFLKTWLMKIAINCALDV
MKKRKNVIHLMPEFEESIGSTETDYAITMTLADMMDSLHEDERSVVVLKYYQDYTFQQISDLLEWPIGTVKTTLYRAVDK
LRREWKEVDCYGS

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. Positively regulates the expression of proteins involved in stress responses against bacitracin, paraquat and tellurite

COG id: COG1595

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit, sigma24 homolog

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family. ECF subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788926, Length=169, Percent_Identity=26.6272189349112, Blast_Score=64, Evalue=5e-12,

Paralogues:

None

Copy number: <10 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000838
- InterPro:   IPR007627
- InterPro:   IPR013249
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR011991 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF08281 Sigma70_r4_2 [H]

EC number: NA

Molecular weight: Translated: 20468; Mature: 20468

Theoretical pI: Translated: 6.28; Mature: 6.28

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
4.6 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRLIQKAQRGNDQAFLKIFQHYEADLYRMAYVYVKNPDDALDVVQEVAYQAFKRIGTLR
CHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHEEECCCCHHHHHHHHHHHHHHHHHCCCC
EPQFLKTWLMKIAINCALDVMKKRKNVIHLMPEFEESIGSTETDYAITMTLADMMDSLHE
CCHHHHHHHHHHHHHHHHHHHHHHHHHEEECCHHHHHCCCCCCCHHHHHHHHHHHHHHCC
DERSVVVLKYYQDYTFQQISDLLEWPIGTVKTTLYRAVDKLRREWKEVDCYGS
CCCCEEEEHHHHCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MKRLIQKAQRGNDQAFLKIFQHYEADLYRMAYVYVKNPDDALDVVQEVAYQAFKRIGTLR
CHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHEEECCCCHHHHHHHHHHHHHHHHHCCCC
EPQFLKTWLMKIAINCALDVMKKRKNVIHLMPEFEESIGSTETDYAITMTLADMMDSLHE
CCHHHHHHHHHHHHHHHHHHHHHHHHHEEECCHHHHHCCCCCCCHHHHHHHHHHHHHHCC
DERSVVVLKYYQDYTFQQISDLLEWPIGTVKTTLYRAVDKLRREWKEVDCYGS
CCCCEEEEHHHHCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9308178; 9384377 [H]