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The map label for this gene is purA

Identifier: 229917476

GI number: 229917476

Start: 1718735

End: 1720024

Strand: Direct

Name: purA

Synonym: EAT1b_1751

Alternate gene names: 229917476

Gene position: 1718735-1720024 (Clockwise)

Preceding gene: 229917475

Following gene: 229917477

Centisome position: 57.29

GC content: 51.47

Gene sequence:

>1290_bases
ATGTCATCAGTAGTCGTAGTGGGAACGCAGTGGGGCGATGAAGGGAAAGGGAAGATCACCGATTTCTTATCGAAACAAGC
CGAAGTCGTCGCGCGTTATCAAGGGGGAGACAACGCTGGTCACACGATCGTCTTCAATGATACGAAATATAAACTTCACC
TCATTCCGTCTGGGATTTTCTATTCGGATAAGACGTGTGTCATCGGGAACGGGATGGTCGTCAACCCGAAATCACTCGTG
ACAGAACTCGCTTACTTACATGAACGTGGTGTGAGCACGGATAACCTTCGAATCTCGAACCGCGCGCATATCATCTTGCC
GTACCACCAGCTTCAAGATCGTCTCGAAGAAGAGGCGAAAGGTGACGCGAAAGTCGGGACGACACTAAAAGGAATCGGCC
CAGCATATATGGATAAGGCGGCACGTATCGGGATTCGCATCGCCGACCTTCTCGACAAAGAAGTGTTCGCGGAGAAGCTA
AAGACCGTGCTTGAACTAAAAAACCGCATGTTCGTGAAAATGTATGAAGTCGATCCAATCGAGTTCGACGACATCTTTCA
AGAATACTATGCGTACGGGCAACAGTTCGCGAAATACGTGTGTGATACATCGGTCGTCTTGAACGATTCACTCGATGAAG
AGAAGAAGGTTCTCTTTGAAGGGGCTCAGGGGGTCCTTCTCGATATCGACCATGGAACGTATCCGTTCGTCACATCGTCG
AACGCAGCGTCAGGTGGCGTATCGAGTGGTGCCGGAATCGGGCCATCGAAGATTCATCACGTGGTCGGCGTCTGTAAAGC
ATACACGTCACGCGTCGGTGACGGTCCGTTCCCGACAGAACTCGACGATGAGATCGGTCATACGATCCGTGAAGTCGGGA
AAGAATACGGAACGACAACTGGTCGTCCACGCCGTGTCGGCTGGTTCGACTCGGTCGTCGTGCGTCACTCACGCCGCGTC
AGCGGGATCACCGATCTTTGCCTCAACTCCATCGATGTATTGACAGGTCTTGAGACGCTCAAGATTTGTACATCGTATGA
GTACGAAGGAAAGCATCTCGACGAGTATCCACCGAACTTCCGCGTCCTTGAGAAGTGTGTACCGGTATACGAAGAGCTTC
CTGGCTGGACAGAAGACATCACAGGGGTTCGTCGCTTTGAAGATCTTCCGGAGAACGCACAGCGTTACGTGCGTCGCATC
GAGGAATTGACAGGAATCGAGTTGTTGACGTTCTCGGTCGGACCGGCTCGTGAACAGACAGTCATCTTGCGTGATATTTA
CGAAGCATAA

Upstream 100 bases:

>100_bases
TCTTATATAAAACGTTCGGTATTCAATTGACTTTCTCGCTTTTCAAGTGTACACTTACGGATGGTTTGAACAGAACGTTG
ATTAATAAGGAGGATTCATT

Downstream 100 bases:

>100_bases
GAAAGGGAGCCATCGGGCTCTCTTTTTCTATTTCCACGGAAATGGACCACGCTTTCTAAGCGGTTTAAAAAGAGTTACAA
TGTAGGCATGTACGTTTAAT

Product: adenylosuccinate synthetase

Products: NA

Alternate protein names: AMPSase; AdSS; IMP--aspartate ligase

Number of amino acids: Translated: 429; Mature: 428

Protein sequence:

>429_residues
MSSVVVVGTQWGDEGKGKITDFLSKQAEVVARYQGGDNAGHTIVFNDTKYKLHLIPSGIFYSDKTCVIGNGMVVNPKSLV
TELAYLHERGVSTDNLRISNRAHIILPYHQLQDRLEEEAKGDAKVGTTLKGIGPAYMDKAARIGIRIADLLDKEVFAEKL
KTVLELKNRMFVKMYEVDPIEFDDIFQEYYAYGQQFAKYVCDTSVVLNDSLDEEKKVLFEGAQGVLLDIDHGTYPFVTSS
NAASGGVSSGAGIGPSKIHHVVGVCKAYTSRVGDGPFPTELDDEIGHTIREVGKEYGTTTGRPRRVGWFDSVVVRHSRRV
SGITDLCLNSIDVLTGLETLKICTSYEYEGKHLDEYPPNFRVLEKCVPVYEELPGWTEDITGVRRFEDLPENAQRYVRRI
EELTGIELLTFSVGPAREQTVILRDIYEA

Sequences:

>Translated_429_residues
MSSVVVVGTQWGDEGKGKITDFLSKQAEVVARYQGGDNAGHTIVFNDTKYKLHLIPSGIFYSDKTCVIGNGMVVNPKSLV
TELAYLHERGVSTDNLRISNRAHIILPYHQLQDRLEEEAKGDAKVGTTLKGIGPAYMDKAARIGIRIADLLDKEVFAEKL
KTVLELKNRMFVKMYEVDPIEFDDIFQEYYAYGQQFAKYVCDTSVVLNDSLDEEKKVLFEGAQGVLLDIDHGTYPFVTSS
NAASGGVSSGAGIGPSKIHHVVGVCKAYTSRVGDGPFPTELDDEIGHTIREVGKEYGTTTGRPRRVGWFDSVVVRHSRRV
SGITDLCLNSIDVLTGLETLKICTSYEYEGKHLDEYPPNFRVLEKCVPVYEELPGWTEDITGVRRFEDLPENAQRYVRRI
EELTGIELLTFSVGPAREQTVILRDIYEA
>Mature_428_residues
SSVVVVGTQWGDEGKGKITDFLSKQAEVVARYQGGDNAGHTIVFNDTKYKLHLIPSGIFYSDKTCVIGNGMVVNPKSLVT
ELAYLHERGVSTDNLRISNRAHIILPYHQLQDRLEEEAKGDAKVGTTLKGIGPAYMDKAARIGIRIADLLDKEVFAEKLK
TVLELKNRMFVKMYEVDPIEFDDIFQEYYAYGQQFAKYVCDTSVVLNDSLDEEKKVLFEGAQGVLLDIDHGTYPFVTSSN
AASGGVSSGAGIGPSKIHHVVGVCKAYTSRVGDGPFPTELDDEIGHTIREVGKEYGTTTGRPRRVGWFDSVVVRHSRRVS
GITDLCLNSIDVLTGLETLKICTSYEYEGKHLDEYPPNFRVLEKCVPVYEELPGWTEDITGVRRFEDLPENAQRYVRRIE
ELTGIELLTFSVGPAREQTVILRDIYEA

Specific function: Plays an important role in the de novo pathway of purine nucleotide biosynthesis. Catalyzes the first commited step in the biosynthesis of AMP from IMP

COG id: COG0104

COG function: function code F; Adenylosuccinate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the adenylosuccinate synthetase family

Homologues:

Organism=Homo sapiens, GI22748717, Length=426, Percent_Identity=42.9577464788732, Blast_Score=342, Evalue=5e-94,
Organism=Homo sapiens, GI34577063, Length=428, Percent_Identity=42.5233644859813, Blast_Score=335, Evalue=6e-92,
Organism=Homo sapiens, GI40316944, Length=397, Percent_Identity=41.8136020151133, Blast_Score=301, Evalue=7e-82,
Organism=Escherichia coli, GI1790620, Length=430, Percent_Identity=46.9767441860465, Blast_Score=414, Evalue=1e-117,
Organism=Caenorhabditis elegans, GI25146553, Length=427, Percent_Identity=41.6861826697892, Blast_Score=327, Evalue=1e-89,
Organism=Caenorhabditis elegans, GI25146555, Length=427, Percent_Identity=41.6861826697892, Blast_Score=326, Evalue=2e-89,
Organism=Saccharomyces cerevisiae, GI6324109, Length=429, Percent_Identity=42.1911421911422, Blast_Score=352, Evalue=8e-98,
Organism=Drosophila melanogaster, GI21356033, Length=421, Percent_Identity=42.5178147268409, Blast_Score=337, Evalue=1e-92,

Paralogues:

None

Copy number: 940 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): PURA_EXISA (C4L014)

Other databases:

- EMBL:   CP001615
- RefSeq:   YP_002886122.1
- GeneID:   7870798
- GenomeReviews:   CP001615_GR
- KEGG:   eat:EAT1b_1751
- OMA:   DYVVRYQ
- ProtClustDB:   CLSK2613248
- GO:   GO:0005737
- HAMAP:   MF_00011
- InterPro:   IPR018220
- InterPro:   IPR001114
- PANTHER:   PTHR11846
- SMART:   SM00788
- TIGRFAMs:   TIGR00184

Pfam domain/function: PF00709 Adenylsucc_synt

EC number: =6.3.4.4

Molecular weight: Translated: 47833; Mature: 47702

Theoretical pI: Translated: 5.15; Mature: 5.15

Prosite motif: PS01266 ADENYLOSUCCIN_SYN_1; PS00513 ADENYLOSUCCIN_SYN_2

Important sites: ACT_SITE 13-13 ACT_SITE 41-41 BINDING 128-128 BINDING 142-142 BINDING 223-223 BINDING 238-238 BINDING 302-302 BINDING 304-304

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSVVVVGTQWGDEGKGKITDFLSKQAEVVARYQGGDNAGHTIVFNDTKYKLHLIPSGIF
CCCEEEEEECCCCCCCCHHHHHHHHHHHHHHEECCCCCCCCEEEECCCEEEEEEEECCEE
YSDKTCVIGNGMVVNPKSLVTELAYLHERGVSTDNLRISNRAHIILPYHQLQDRLEEEAK
ECCCEEEECCCEEECHHHHHHHHHHHHHCCCCCCCEEECCCEEEEECHHHHHHHHHHHHC
GDAKVGTTLKGIGPAYMDKAARIGIRIADLLDKEVFAEKLKTVLELKNRMFVKMYEVDPI
CCCHHCCCHHCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCC
EFDDIFQEYYAYGQQFAKYVCDTSVVLNDSLDEEKKVLFEGAQGVLLDIDHGTYPFVTSS
CHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCHHHHHHHCCCCEEEEEECCCCCCEEECC
NAASGGVSSGAGIGPSKIHHVVGVCKAYTSRVGDGPFPTELDDEIGHTIREVGKEYGTTT
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCC
GRPRRVGWFDSVVVRHSRRVSGITDLCLNSIDVLTGLETLKICTSYEYEGKHLDEYPPNF
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCH
RVLEKCVPVYEELPGWTEDITGVRRFEDLPENAQRYVRRIEELTGIELLTFSVGPAREQT
HHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCEEEEEECCCCCCCE
VILRDIYEA
EHHHHHHCC
>Mature Secondary Structure 
SSVVVVGTQWGDEGKGKITDFLSKQAEVVARYQGGDNAGHTIVFNDTKYKLHLIPSGIF
CCEEEEEECCCCCCCCHHHHHHHHHHHHHHEECCCCCCCCEEEECCCEEEEEEEECCEE
YSDKTCVIGNGMVVNPKSLVTELAYLHERGVSTDNLRISNRAHIILPYHQLQDRLEEEAK
ECCCEEEECCCEEECHHHHHHHHHHHHHCCCCCCCEEECCCEEEEECHHHHHHHHHHHHC
GDAKVGTTLKGIGPAYMDKAARIGIRIADLLDKEVFAEKLKTVLELKNRMFVKMYEVDPI
CCCHHCCCHHCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCC
EFDDIFQEYYAYGQQFAKYVCDTSVVLNDSLDEEKKVLFEGAQGVLLDIDHGTYPFVTSS
CHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCHHHHHHHCCCCEEEEEECCCCCCEEECC
NAASGGVSSGAGIGPSKIHHVVGVCKAYTSRVGDGPFPTELDDEIGHTIREVGKEYGTTT
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCC
GRPRRVGWFDSVVVRHSRRVSGITDLCLNSIDVLTGLETLKICTSYEYEGKHLDEYPPNF
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCH
RVLEKCVPVYEELPGWTEDITGVRRFEDLPENAQRYVRRIEELTGIELLTFSVGPAREQT
HHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCEEEEEECCCCCCCE
VILRDIYEA
EHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA