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The map label for this gene is dnaN [H]

Identifier: 229917454

GI number: 229917454

Start: 1697774

End: 1698913

Strand: Reverse

Name: dnaN [H]

Synonym: EAT1b_1729

Alternate gene names: 229917454

Gene position: 1698913-1697774 (Counterclockwise)

Preceding gene: 229917455

Following gene: 229917453

Centisome position: 56.63

GC content: 47.19

Gene sequence:

>1140_bases
ATGCATTTAACGATTCAACGAGATGTATTCATGCAAGCCGTACAAGACGTATCAAAAGCTGTGGCTTCACGAACGACCAT
TCCAATTTTGACAGGCTTGAAACTTGAGGCACATGCGGATGGCTTAACACTGACAGGAAGTGATACCGAGATTTCGATTG
AACGCTTGATTCCAATCGAAGAGGGTGGAATGGAACTCATTCAAATTCAACGTGCTGGAAGCGTCGTGTTGAACGCACGC
TTCTTAGGAGAAATCGTGAAGAAGTTACCGACGAACGAGGTCACACTCGAAGTTTCACCGAACTTCATGACGCGCATTGA
GTCTGGTCAGGCAGAGTTCCATTTAAACGGTCTCGACCCGGATGAATATCCACGCCTACCTGAACTTGCGAGTGATCGTC
GCTTCTATTTGCCGGCTGATCTATTGAAGACGATCATCCGTCAGACAAGCTTTGCTGTTTCTTCACAAGAGACACGTCCC
GTATTGACAGGGGTTAACTTCTCAGCAGAGAAGGGCTTCCTTACATGTGTCGCAACCGATAGCCACCGTCTGGCGCTTCG
TCGGGCGCGTTTTGAGACAGATAACGATTTGACGTTCCAAAATGTCGTCGTTCCTGGTCGCTCACTCAACGAACTCGCTA
AATTACTCGACCGCGAAGAGTTGCCGATCGAAATCGTCTTAACAGACCAACAAATTTTATTCCGTATGAAAAACATCTCA
TTCTTCTCTCGTTTGTTAGACGGTGCCTACCCAGACACGTCACGTCTCATTCCGGAAGAATATCGCACAGCGGTTCGAAT
GAATGCGAAAGAATTCTTGCAGGCCATTGATCGTGCATCGCTCTTAGCACGTGCCGATCGCAACAACGTCATTAAATTCG
TGGCGGAAGATAAAACAGCAGTTGAAGTCTCTTCTCACTCGCCTGAAGTCGGAAAAGTATCAGAACGTGTCAGCATCCTG
TCGCTTGAAGGAGAAGAACTAAAAATTTCCTTCAACTCAAAATTCGTGATGGACGCGCTCCGCGCACTCGATGCATCTGA
AATCGAAATCCAGTTCACGGGTTCGATGCGACCGTTCATTTTACGACCGGTTGAACAAGATAGTGTTCTTCAACTGATTT
TGCCAGTCCGCACATCGTAA

Upstream 100 bases:

>100_bases
TATCCCCAGTATCCACAGCCCTTATTACTATTATGACTTAATTAATACTATTATTATTATTAAGAAATGGACTCATTTCA
CCTTAGGAGGAATTATCCAC

Downstream 100 bases:

>100_bases
GCTGCGGATAGGGATGATGAGGTGTCTTGCCTTGTCATCCCTCTTTTTGCCTTATTCGACGATTTTCGATAAACTAATAT
GTATGGCGATTTTTTCAGAT

Product: DNA polymerase III subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 379; Mature: 379

Protein sequence:

>379_residues
MHLTIQRDVFMQAVQDVSKAVASRTTIPILTGLKLEAHADGLTLTGSDTEISIERLIPIEEGGMELIQIQRAGSVVLNAR
FLGEIVKKLPTNEVTLEVSPNFMTRIESGQAEFHLNGLDPDEYPRLPELASDRRFYLPADLLKTIIRQTSFAVSSQETRP
VLTGVNFSAEKGFLTCVATDSHRLALRRARFETDNDLTFQNVVVPGRSLNELAKLLDREELPIEIVLTDQQILFRMKNIS
FFSRLLDGAYPDTSRLIPEEYRTAVRMNAKEFLQAIDRASLLARADRNNVIKFVAEDKTAVEVSSHSPEVGKVSERVSIL
SLEGEELKISFNSKFVMDALRALDASEIEIQFTGSMRPFILRPVEQDSVLQLILPVRTS

Sequences:

>Translated_379_residues
MHLTIQRDVFMQAVQDVSKAVASRTTIPILTGLKLEAHADGLTLTGSDTEISIERLIPIEEGGMELIQIQRAGSVVLNAR
FLGEIVKKLPTNEVTLEVSPNFMTRIESGQAEFHLNGLDPDEYPRLPELASDRRFYLPADLLKTIIRQTSFAVSSQETRP
VLTGVNFSAEKGFLTCVATDSHRLALRRARFETDNDLTFQNVVVPGRSLNELAKLLDREELPIEIVLTDQQILFRMKNIS
FFSRLLDGAYPDTSRLIPEEYRTAVRMNAKEFLQAIDRASLLARADRNNVIKFVAEDKTAVEVSSHSPEVGKVSERVSIL
SLEGEELKISFNSKFVMDALRALDASEIEIQFTGSMRPFILRPVEQDSVLQLILPVRTS
>Mature_379_residues
MHLTIQRDVFMQAVQDVSKAVASRTTIPILTGLKLEAHADGLTLTGSDTEISIERLIPIEEGGMELIQIQRAGSVVLNAR
FLGEIVKKLPTNEVTLEVSPNFMTRIESGQAEFHLNGLDPDEYPRLPELASDRRFYLPADLLKTIIRQTSFAVSSQETRP
VLTGVNFSAEKGFLTCVATDSHRLALRRARFETDNDLTFQNVVVPGRSLNELAKLLDREELPIEIVLTDQQILFRMKNIS
FFSRLLDGAYPDTSRLIPEEYRTAVRMNAKEFLQAIDRASLLARADRNNVIKFVAEDKTAVEVSSHSPEVGKVSERVSIL
SLEGEELKISFNSKFVMDALRALDASEIEIQFTGSMRPFILRPVEQDSVLQLILPVRTS

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The beta chain is required for initiation of replication once it is clamped

COG id: COG0592

COG function: function code L; DNA polymerase sliding clamp subunit (PCNA homolog)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1790136, Length=378, Percent_Identity=27.5132275132275, Blast_Score=159, Evalue=3e-40,

Paralogues:

None

Copy number: 5954 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001001
- InterPro:   IPR022635
- InterPro:   IPR022637
- InterPro:   IPR022634 [H]

Pfam domain/function: PF00712 DNA_pol3_beta; PF02767 DNA_pol3_beta_2; PF02768 DNA_pol3_beta_3 [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 42484; Mature: 42484

Theoretical pI: Translated: 5.01; Mature: 5.01

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHLTIQRDVFMQAVQDVSKAVASRTTIPILTGLKLEAHADGLTLTGSDTEISIERLIPIE
CEEEEHHHHHHHHHHHHHHHHHCCCCEEEEECEEEEECCCCEEEECCCCEEEEEEEEEEC
EGGMELIQIQRAGSVVLNARFLGEIVKKLPTNEVTLEVSPNFMTRIESGQAEFHLNGLDP
CCCCEEEEEECCCCEEEEHHHHHHHHHHCCCCCEEEEECCHHHHHCCCCCCEEEECCCCC
DEYPRLPELASDRRFYLPADLLKTIIRQTSFAVSSQETRPVLTGVNFSAEKGFLTCVATD
CCCCCCCCHHCCCEEEECHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCCCCEEEEEECC
SHRLALRRARFETDNDLTFQNVVVPGRSLNELAKLLDREELPIEIVLTDQQILFRMKNIS
CHHHHHHHHHCCCCCCCEEEEEEECCCCHHHHHHHHCCCCCCEEEEEECHHHHHHHHHHH
FFSRLLDGAYPDTSRLIPEEYRTAVRMNAKEFLQAIDRASLLARADRNNVIKFVAEDKTA
HHHHHHCCCCCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCEEEEEECCCEE
VEVSSHSPEVGKVSERVSILSLEGEELKISFNSKFVMDALRALDASEIEIQFTGSMRPFI
EEECCCCCCCCHHHCCEEEEEECCCEEEEEECCHHHHHHHHHCCCCEEEEEEECCCCCEE
LRPVEQDSVLQLILPVRTS
ECCCCCCCEEEEEEECCCC
>Mature Secondary Structure
MHLTIQRDVFMQAVQDVSKAVASRTTIPILTGLKLEAHADGLTLTGSDTEISIERLIPIE
CEEEEHHHHHHHHHHHHHHHHHCCCCEEEEECEEEEECCCCEEEECCCCEEEEEEEEEEC
EGGMELIQIQRAGSVVLNARFLGEIVKKLPTNEVTLEVSPNFMTRIESGQAEFHLNGLDP
CCCCEEEEEECCCCEEEEHHHHHHHHHHCCCCCEEEEECCHHHHHCCCCCCEEEECCCCC
DEYPRLPELASDRRFYLPADLLKTIIRQTSFAVSSQETRPVLTGVNFSAEKGFLTCVATD
CCCCCCCCHHCCCEEEECHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCCCCEEEEEECC
SHRLALRRARFETDNDLTFQNVVVPGRSLNELAKLLDREELPIEIVLTDQQILFRMKNIS
CHHHHHHHHHCCCCCCCEEEEEEECCCCHHHHHHHHCCCCCCEEEEEECHHHHHHHHHHH
FFSRLLDGAYPDTSRLIPEEYRTAVRMNAKEFLQAIDRASLLARADRNNVIKFVAEDKTA
HHHHHHCCCCCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCEEEEEECCCEE
VEVSSHSPEVGKVSERVSILSLEGEELKISFNSKFVMDALRALDASEIEIQFTGSMRPFI
EEECCCCCCCCHHHCCEEEEEECCCEEEEEECCHHHHHHHHHCCCCEEEEEEECCCCCEE
LRPVEQDSVLQLILPVRTS
ECCCCCCCEEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2987847; 7584024; 9384377; 2846289 [H]