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The map label for this gene is recF

Identifier: 229917452

GI number: 229917452

Start: 1696312

End: 1697430

Strand: Reverse

Name: recF

Synonym: EAT1b_1727

Alternate gene names: 229917452

Gene position: 1697430-1696312 (Counterclockwise)

Preceding gene: 229917453

Following gene: 229917451

Centisome position: 56.58

GC content: 44.86

Gene sequence:

>1119_bases
GTGCATTTAAAGTCGATCCGATTGCAAAACTATCGCAACTACGAAACCCTCGAGCTAGATTTTTCAGAACAGACGAATGT
TCTGATTGGTGAGAACGCCCAAGGAAAAACGAATCTTCTCGAATCGATTTACGTACTTGCGCTCGCAAAATCGCATCGGA
CGACACAAGACCGTGAGTTAATCGGTTGGGAAGCTGATGCTGCGAGTATCGAAGGCCGGATTCATAAACGAACGGGTGAA
TCGGTGCAGAGTCTCTCTTTTTCTCCTAAAGGCAAGAAAGCAAAGCTCAATCATTTGGAACAACGTCGTCTCAGTGACTA
TGTCGGCGCATTTAACGTCGTCTTGTTCGCCCCTGAAGACTTAGCCATCGTCAAAGGCAGTCCGCAAGGGAGACGGCGAT
TTTTAGATATGGAAATCGGTCAGGTCAGTCCCGTGTACTTACACGAGCTCAATCAATACTTGAAGACGTTGAAACAACGG
AATGCCTTGTTGAAGCAGTTATCCACAAAAGGTGGCGACGAGACGTTACTCGAAGTATTGACGGACCAATTGATTGAACT
TGCCGTAAAAATCGTGATGCGACGCTATCACTTCATCGACCAGCTCGAAAAATGGGCCAATCCCATCCATTCCGGAATCA
CGAGAGATCTCGAAACACTGACGATTCAATACGTTTCTGACACGTTCCAAAAAGAACGCTTCTCAAAAGAACAGATGTTT
GAAACGTATCGTCAAAAGTTTGATAAAATAAGAGAGAACGAACGACGAAGAGGTGTGACCTTGTTCGGTCCGCATCGTGA
CGATTTTGAATTGTATGTCAACAATCGAAACGTTCAAACGTTCGGTTCACAAGGTCAGCAACGAACGGCGGCCTTGTCAT
TGAAGCTAGCTGAGATTGAATTGATTCATGAAGAAGTAGGCGAATATCCTCTGTTGTTATTAGACGATGTGTTATCTGAG
CTTGACGATCATCGCCAAACCCATCTCCTCGATACGATGGGACGGAAAGTACAAACGATTTTGACTACAACAAACATTGA
CGGGATTGCACATGAGACCATTCAACAAGCTAAAGTGTTTCACGTGAAACAAGGTGAGGTTGAAACGGAGTCTGTGTAA

Upstream 100 bases:

>100_bases
GTGAACGGTGAGCAGGACCAACGCCGTGGTCGTAAATTAAGAGATGGAGATACAATCGAAGTCGAAGGCTTCGGGGAATA
TCAAATCGAGGGAAACTGAC

Downstream 100 bases:

>100_bases
TCGTGGCAAGAGGGAACACCAAATCATGTATAGTAGGTGAAAATAACCGATGAGTAATGAAAATGAAAAAGAATTGCAAG
CGTACGGCGCCGACCAGATT

Product: DNA replication and repair protein RecF

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 372; Mature: 372

Protein sequence:

>372_residues
MHLKSIRLQNYRNYETLELDFSEQTNVLIGENAQGKTNLLESIYVLALAKSHRTTQDRELIGWEADAASIEGRIHKRTGE
SVQSLSFSPKGKKAKLNHLEQRRLSDYVGAFNVVLFAPEDLAIVKGSPQGRRRFLDMEIGQVSPVYLHELNQYLKTLKQR
NALLKQLSTKGGDETLLEVLTDQLIELAVKIVMRRYHFIDQLEKWANPIHSGITRDLETLTIQYVSDTFQKERFSKEQMF
ETYRQKFDKIRENERRRGVTLFGPHRDDFELYVNNRNVQTFGSQGQQRTAALSLKLAEIELIHEEVGEYPLLLLDDVLSE
LDDHRQTHLLDTMGRKVQTILTTTNIDGIAHETIQQAKVFHVKQGEVETESV

Sequences:

>Translated_372_residues
MHLKSIRLQNYRNYETLELDFSEQTNVLIGENAQGKTNLLESIYVLALAKSHRTTQDRELIGWEADAASIEGRIHKRTGE
SVQSLSFSPKGKKAKLNHLEQRRLSDYVGAFNVVLFAPEDLAIVKGSPQGRRRFLDMEIGQVSPVYLHELNQYLKTLKQR
NALLKQLSTKGGDETLLEVLTDQLIELAVKIVMRRYHFIDQLEKWANPIHSGITRDLETLTIQYVSDTFQKERFSKEQMF
ETYRQKFDKIRENERRRGVTLFGPHRDDFELYVNNRNVQTFGSQGQQRTAALSLKLAEIELIHEEVGEYPLLLLDDVLSE
LDDHRQTHLLDTMGRKVQTILTTTNIDGIAHETIQQAKVFHVKQGEVETESV
>Mature_372_residues
MHLKSIRLQNYRNYETLELDFSEQTNVLIGENAQGKTNLLESIYVLALAKSHRTTQDRELIGWEADAASIEGRIHKRTGE
SVQSLSFSPKGKKAKLNHLEQRRLSDYVGAFNVVLFAPEDLAIVKGSPQGRRRFLDMEIGQVSPVYLHELNQYLKTLKQR
NALLKQLSTKGGDETLLEVLTDQLIELAVKIVMRRYHFIDQLEKWANPIHSGITRDLETLTIQYVSDTFQKERFSKEQMF
ETYRQKFDKIRENERRRGVTLFGPHRDDFELYVNNRNVQTFGSQGQQRTAALSLKLAEIELIHEEVGEYPLLLLDDVLSE
LDDHRQTHLLDTMGRKVQTILTTTNIDGIAHETIQQAKVFHVKQGEVETESV

Specific function: The recF protein is involved in DNA metabolism; it is required for DNA replication and normal SOS inducibility. RecF binds preferentially to single-stranded, linear DNA. It also seems to bind ATP

COG id: COG1195

COG function: function code L; Recombinational DNA repair ATPase (RecF pathway)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recF family

Homologues:

Organism=Escherichia coli, GI1790135, Length=370, Percent_Identity=26.4864864864865, Blast_Score=135, Evalue=3e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RECF_EXISA (C4KZZ0)

Other databases:

- EMBL:   CP001615
- RefSeq:   YP_002886098.1
- GeneID:   7870774
- GenomeReviews:   CP001615_GR
- KEGG:   eat:EAT1b_1727
- OMA:   GQQRTLV
- ProtClustDB:   CLSK2488810
- GO:   GO:0005694
- GO:   GO:0005737
- HAMAP:   MF_00365
- InterPro:   IPR001238
- InterPro:   IPR018078
- InterPro:   IPR003395
- TIGRFAMs:   TIGR00611

Pfam domain/function: PF02463 SMC_N

EC number: NA

Molecular weight: Translated: 42931; Mature: 42931

Theoretical pI: Translated: 6.72; Mature: 6.72

Prosite motif: PS00617 RECF_1; PS00618 RECF_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHLKSIRLQNYRNYETLELDFSEQTNVLIGENAQGKTNLLESIYVLALAKSHRTTQDREL
CCCCCCHHCCCCCCEEEEEECCCCCCEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCHH
IGWEADAASIEGRIHKRTGESVQSLSFSPKGKKAKLNHLEQRRLSDYVGAFNVVLFAPED
CCCCCCHHHHCCHHHHHCCCHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHEEEEEECCCC
LAIVKGSPQGRRRFLDMEIGQVSPVYLHELNQYLKTLKQRNALLKQLSTKGGDETLLEVL
EEEEECCCHHHHEEHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
TDQLIELAVKIVMRRYHFIDQLEKWANPIHSGITRDLETLTIQYVSDTFQKERFSKEQMF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHH
ETYRQKFDKIRENERRRGVTLFGPHRDDFELYVNNRNVQTFGSQGQQRTAALSLKLAEIE
HHHHHHHHHHHHHHHHCCCEEECCCCCCEEEEEECCCEEECCCCCCHHHHHHHHHHHHHH
LIHEEVGEYPLLLLDDVLSELDDHRQTHLLDTMGRKVQTILTTTNIDGIAHETIQQAKVF
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCHHHHHHHHHHHHE
HVKQGEVETESV
EEECCCCCCCCC
>Mature Secondary Structure
MHLKSIRLQNYRNYETLELDFSEQTNVLIGENAQGKTNLLESIYVLALAKSHRTTQDREL
CCCCCCHHCCCCCCEEEEEECCCCCCEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCHH
IGWEADAASIEGRIHKRTGESVQSLSFSPKGKKAKLNHLEQRRLSDYVGAFNVVLFAPED
CCCCCCHHHHCCHHHHHCCCHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHEEEEEECCCC
LAIVKGSPQGRRRFLDMEIGQVSPVYLHELNQYLKTLKQRNALLKQLSTKGGDETLLEVL
EEEEECCCHHHHEEHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
TDQLIELAVKIVMRRYHFIDQLEKWANPIHSGITRDLETLTIQYVSDTFQKERFSKEQMF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHH
ETYRQKFDKIRENERRRGVTLFGPHRDDFELYVNNRNVQTFGSQGQQRTAALSLKLAEIE
HHHHHHHHHHHHHHHHCCCEEECCCCCCEEEEEECCCEEECCCCCCHHHHHHHHHHHHHH
LIHEEVGEYPLLLLDDVLSELDDHRQTHLLDTMGRKVQTILTTTNIDGIAHETIQQAKVF
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCHHHHHHHHHHHHE
HVKQGEVETESV
EEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA