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The map label for this gene is gyrB [H]

Identifier: 229917451

GI number: 229917451

Start: 1694334

End: 1696262

Strand: Reverse

Name: gyrB [H]

Synonym: EAT1b_1726

Alternate gene names: 229917451

Gene position: 1696262-1694334 (Counterclockwise)

Preceding gene: 229917452

Following gene: 229917450

Centisome position: 56.54

GC content: 50.03

Gene sequence:

>1929_bases
ATGAGTAATGAAAATGAAAAAGAATTGCAAGCGTACGGCGCCGACCAGATTCAAGTCCTTGAGGGGCTAGAGGCTGTCCG
TAAACGTCCAGGGATGTATATCGGTTCCACAAGCTCTCGCGGTCTCCACCATTTAGTATGGGAGATTGTCGATAACTCAA
TCGATGAGGCGCTCGCTGGTTATTGTGACACGATCACAGTGACGGTCGAGCCAGGGAACTCGATCGTTGTTGAAGACAAC
GGACGTGGGATTCCGGTCGACGTTCAAAAGAAAACAGGTCGACCTGCCGTTGAAGTTATTTTCACGGTGCTTCACGCAGG
TGGTAAGTTCGGCGGTGGCGGATACAAAGTATCAGGTGGTCTTCACGGTGTCGGGGCGTCGGTTGTTAACGCCTTATCGA
CGAAACTTGAAGTGTTCGTTCGTCGCGATGGAAACGTATATTACCAATCGTTCAAACGTGGTGTTCCACAAGGTGAACTC
GAAATCGTCGGGACGACCGACACGACAGGGACGACGGTCCGTTTCTTCCCAGACGGGGACATCTTCCAAGAGACACTCGA
ATATGACTTTGATCTGTTAGCGACACGCTTACGTGAACTCGCCTTCTTGAACAAAGGGTTACGAATCGTGGCTAAAGATG
ATCGTAATGACGAGACGATTGAACGGAACTATCACTATGAGGGCGGAATCAAGTCTTATGTCGAGCATTTGAACCGGTCA
AAAGCGTCGATTCATGAAGAGCCTGTGTACGTACACGGGACGAAAGACGGAATCGAAGTCGAGATTGCGCTTCAATACAA
CGAAGGTTTCGCAAGTAACATTTACTCATTTACGAACAACATTCCGACGCATGAAGGTGGGACGCACGAGACAGGATTTA
AGACGGCACTCACTCGTTCAATCAACGACTATGCAAAACGGTTTGGTTTGATGAAAGAAGCCGACGGCAGCCTGTCTGGT
GATGATGTCCGTGAAGGGTTGACGGCGATTGTTTCCGTCAAGCACCCGGCTCCCCAGTTTGAAGGACAGACGAAGACGAA
ACTCGGGAATGCCGATGCGCGTACGGCAACCGATACGTTGTTCAGCGACACGTTCGAACAGTTCCTGCTCGAGAACCCAT
CGGTCGGTCGCCTCATCGTCGACAAAGGCTTGATGGCGGCACGTGCGCGCCTTGCTGCGAAACGTGCACGCGAGATGACA
CGTCGTAAAGGGATTCTCGAAGTCAGTTCATTGCCAGGGAAATTGGCAGACTGTTCGTCAAAAGATGCATCGAAATCTGA
ACTATACATCGTTGAGGGTGACTCGGCGGGAGGTTCTGCGAAATCTGGACGTGATCGTCACTTCCAAGCCATCCTTCCGA
TTCGAGGGAAAATCATCAACGTTGAGAAAGCACGTCTTGATAAGATTTTGGCCAACCAAGAAGTCCGGACCATCATCACG
GCTCTCGGAACAGGGATTGCCGATGAGTTTGACTTATCGAAAGCACGCTATCACAAACTCATCATCATGACCGATGCCGA
TGTCGATGGTGCGCACATTCGGACGTTGTTGTTGACGTTCTTCTATCGTTACATGCGCCCACTCGTTGAAAGTGGATATG
TCTATATCGCGCAACCACCACTCTATGGTGTGAAGCACGGGAAAGAAACCATCTACGTTCAAAACGACCGGGAGTTGCAG
GCGGCGATTGCACAACTTCCTGAAAATGCACGTTATAGCGTTCAACGTTACAAGGGTCTCGGAGAGATGGATCCTGAACA
GTTATGGAATACGACGATGAACCCGGAAACGCGTTCGATGCTTCGTGTCGATCTTCAAGATGCGATCGAAGCGGATGAAA
TCTTCGAAACGCTCATGGGTGACAACGTCGAACCACGCCGTGACTTTATTCAATCTCACGCCCATTACGTGAAGAACCTC
GATATTTAA

Upstream 100 bases:

>100_bases
GCTAAAGTGTTTCACGTGAAACAAGGTGAGGTTGAAACGGAGTCTGTGTAATCGTGGCAAGAGGGAACACCAAATCATGT
ATAGTAGGTGAAAATAACCG

Downstream 100 bases:

>100_bases
TAAGAAGGGTAGGCAATAACGAATGGCAGAACAAGAAAATATCAAGGATATAAATATTAGCCAGGAGATGCGAACGTCGT
TCATGGACTACGCGATGAGC

Product: DNA gyrase, B subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 642; Mature: 641

Protein sequence:

>642_residues
MSNENEKELQAYGADQIQVLEGLEAVRKRPGMYIGSTSSRGLHHLVWEIVDNSIDEALAGYCDTITVTVEPGNSIVVEDN
GRGIPVDVQKKTGRPAVEVIFTVLHAGGKFGGGGYKVSGGLHGVGASVVNALSTKLEVFVRRDGNVYYQSFKRGVPQGEL
EIVGTTDTTGTTVRFFPDGDIFQETLEYDFDLLATRLRELAFLNKGLRIVAKDDRNDETIERNYHYEGGIKSYVEHLNRS
KASIHEEPVYVHGTKDGIEVEIALQYNEGFASNIYSFTNNIPTHEGGTHETGFKTALTRSINDYAKRFGLMKEADGSLSG
DDVREGLTAIVSVKHPAPQFEGQTKTKLGNADARTATDTLFSDTFEQFLLENPSVGRLIVDKGLMAARARLAAKRAREMT
RRKGILEVSSLPGKLADCSSKDASKSELYIVEGDSAGGSAKSGRDRHFQAILPIRGKIINVEKARLDKILANQEVRTIIT
ALGTGIADEFDLSKARYHKLIIMTDADVDGAHIRTLLLTFFYRYMRPLVESGYVYIAQPPLYGVKHGKETIYVQNDRELQ
AAIAQLPENARYSVQRYKGLGEMDPEQLWNTTMNPETRSMLRVDLQDAIEADEIFETLMGDNVEPRRDFIQSHAHYVKNL
DI

Sequences:

>Translated_642_residues
MSNENEKELQAYGADQIQVLEGLEAVRKRPGMYIGSTSSRGLHHLVWEIVDNSIDEALAGYCDTITVTVEPGNSIVVEDN
GRGIPVDVQKKTGRPAVEVIFTVLHAGGKFGGGGYKVSGGLHGVGASVVNALSTKLEVFVRRDGNVYYQSFKRGVPQGEL
EIVGTTDTTGTTVRFFPDGDIFQETLEYDFDLLATRLRELAFLNKGLRIVAKDDRNDETIERNYHYEGGIKSYVEHLNRS
KASIHEEPVYVHGTKDGIEVEIALQYNEGFASNIYSFTNNIPTHEGGTHETGFKTALTRSINDYAKRFGLMKEADGSLSG
DDVREGLTAIVSVKHPAPQFEGQTKTKLGNADARTATDTLFSDTFEQFLLENPSVGRLIVDKGLMAARARLAAKRAREMT
RRKGILEVSSLPGKLADCSSKDASKSELYIVEGDSAGGSAKSGRDRHFQAILPIRGKIINVEKARLDKILANQEVRTIIT
ALGTGIADEFDLSKARYHKLIIMTDADVDGAHIRTLLLTFFYRYMRPLVESGYVYIAQPPLYGVKHGKETIYVQNDRELQ
AAIAQLPENARYSVQRYKGLGEMDPEQLWNTTMNPETRSMLRVDLQDAIEADEIFETLMGDNVEPRRDFIQSHAHYVKNL
DI
>Mature_641_residues
SNENEKELQAYGADQIQVLEGLEAVRKRPGMYIGSTSSRGLHHLVWEIVDNSIDEALAGYCDTITVTVEPGNSIVVEDNG
RGIPVDVQKKTGRPAVEVIFTVLHAGGKFGGGGYKVSGGLHGVGASVVNALSTKLEVFVRRDGNVYYQSFKRGVPQGELE
IVGTTDTTGTTVRFFPDGDIFQETLEYDFDLLATRLRELAFLNKGLRIVAKDDRNDETIERNYHYEGGIKSYVEHLNRSK
ASIHEEPVYVHGTKDGIEVEIALQYNEGFASNIYSFTNNIPTHEGGTHETGFKTALTRSINDYAKRFGLMKEADGSLSGD
DVREGLTAIVSVKHPAPQFEGQTKTKLGNADARTATDTLFSDTFEQFLLENPSVGRLIVDKGLMAARARLAAKRAREMTR
RKGILEVSSLPGKLADCSSKDASKSELYIVEGDSAGGSAKSGRDRHFQAILPIRGKIINVEKARLDKILANQEVRTIITA
LGTGIADEFDLSKARYHKLIIMTDADVDGAHIRTLLLTFFYRYMRPLVESGYVYIAQPPLYGVKHGKETIYVQNDRELQA
AIAQLPENARYSVQRYKGLGEMDPEQLWNTTMNPETRSMLRVDLQDAIEADEIFETLMGDNVEPRRDFIQSHAHYVKNLD
I

Specific function: DNA gyrase negatively supercoils closed circular double- stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings [H]

COG id: COG0187

COG function: function code L; Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), B subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI19913406, Length=604, Percent_Identity=26.3245033112583, Blast_Score=147, Evalue=4e-35,
Organism=Homo sapiens, GI19913408, Length=605, Percent_Identity=25.9504132231405, Blast_Score=133, Evalue=6e-31,
Organism=Escherichia coli, GI48994957, Length=559, Percent_Identity=56.8872987477639, Blast_Score=627, Evalue=0.0,
Organism=Escherichia coli, GI1789408, Length=629, Percent_Identity=40.3815580286169, Blast_Score=447, Evalue=1e-127,
Organism=Caenorhabditis elegans, GI17535065, Length=606, Percent_Identity=26.8976897689769, Blast_Score=150, Evalue=2e-36,
Organism=Caenorhabditis elegans, GI212645845, Length=539, Percent_Identity=25.0463821892393, Blast_Score=123, Evalue=3e-28,
Organism=Caenorhabditis elegans, GI212645657, Length=243, Percent_Identity=25.9259259259259, Blast_Score=81, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6324241, Length=624, Percent_Identity=24.1987179487179, Blast_Score=107, Evalue=8e-24,
Organism=Drosophila melanogaster, GI17136538, Length=663, Percent_Identity=24.4343891402715, Blast_Score=131, Evalue=2e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR011557
- InterPro:   IPR020568
- InterPro:   IPR014721
- InterPro:   IPR001241
- InterPro:   IPR013759
- InterPro:   IPR002288
- InterPro:   IPR013506
- InterPro:   IPR013760
- InterPro:   IPR018522
- InterPro:   IPR006171 [H]

Pfam domain/function: PF00204 DNA_gyraseB; PF00986 DNA_gyraseB_C; PF02518 HATPase_c; PF01751 Toprim [H]

EC number: =5.99.1.3 [H]

Molecular weight: Translated: 71225; Mature: 71094

Theoretical pI: Translated: 5.87; Mature: 5.87

Prosite motif: PS00177 TOPOISOMERASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNENEKELQAYGADQIQVLEGLEAVRKRPGMYIGSTSSRGLHHLVWEIVDNSIDEALAG
CCCCCHHHHHHCCCCHHHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHC
YCDTITVTVEPGNSIVVEDNGRGIPVDVQKKTGRPAVEVIFTVLHAGGKFGGGGYKVSGG
CCCEEEEEEECCCEEEEECCCCCCCEECCCCCCCHHHHHHHHHHHCCCCCCCCCEEECCC
LHGVGASVVNALSTKLEVFVRRDGNVYYQSFKRGVPQGELEIVGTTDTTGTTVRFFPDGD
CCHHHHHHHHHHHHHEEEEEECCCCCHHHHHHCCCCCCCEEEEECCCCCCCEEEECCCCH
IFQETLEYDFDLLATRLRELAFLNKGLRIVAKDDRNDETIERNYHYEGGIKSYVEHLNRS
HHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCHHHCCCCCCCCHHHHHHHHCCH
KASIHEEPVYVHGTKDGIEVEIALQYNEGFASNIYSFTNNIPTHEGGTHETGFKTALTRS
HHHHCCCCEEEECCCCCEEEEEEEEECCCHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHH
INDYAKRFGLMKEADGSLSGDDVREGLTAIVSVKHPAPQFEGQTKTKLGNADARTATDTL
HHHHHHHCCCHHCCCCCCCCHHHHHHHHEEEECCCCCCCCCCCCHHCCCCCCCCHHHHHH
FSDTFEQFLLENPSVGRLIVDKGLMAARARLAAKRAREMTRRKGILEVSSLPGKLADCSS
HHHHHHHHHHCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCHHCCCC
KDASKSELYIVEGDSAGGSAKSGRDRHFQAILPIRGKIINVEKARLDKILANQEVRTIIT
CCCCCCEEEEEECCCCCCCCCCCCCCCEEEEECCCCEEEECHHHHHHHHHHCHHHHHHHH
ALGTGIADEFDLSKARYHKLIIMTDADVDGAHIRTLLLTFFYRYMRPLVESGYVYIAQPP
HHHCCCCCHHHCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEECCC
LYGVKHGKETIYVQNDRELQAAIAQLPENARYSVQRYKGLGEMDPEQLWNTTMNPETRSM
CCCCCCCCEEEEEECCHHHHHHHHHCCCCCHHHHHHHCCCCCCCHHHHHCCCCCCCHHHH
LRVDLQDAIEADEIFETLMGDNVEPRRDFIQSHAHYVKNLDI
HHHHHHHHCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SNENEKELQAYGADQIQVLEGLEAVRKRPGMYIGSTSSRGLHHLVWEIVDNSIDEALAG
CCCCHHHHHHCCCCHHHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHC
YCDTITVTVEPGNSIVVEDNGRGIPVDVQKKTGRPAVEVIFTVLHAGGKFGGGGYKVSGG
CCCEEEEEEECCCEEEEECCCCCCCEECCCCCCCHHHHHHHHHHHCCCCCCCCCEEECCC
LHGVGASVVNALSTKLEVFVRRDGNVYYQSFKRGVPQGELEIVGTTDTTGTTVRFFPDGD
CCHHHHHHHHHHHHHEEEEEECCCCCHHHHHHCCCCCCCEEEEECCCCCCCEEEECCCCH
IFQETLEYDFDLLATRLRELAFLNKGLRIVAKDDRNDETIERNYHYEGGIKSYVEHLNRS
HHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCHHHCCCCCCCCHHHHHHHHCCH
KASIHEEPVYVHGTKDGIEVEIALQYNEGFASNIYSFTNNIPTHEGGTHETGFKTALTRS
HHHHCCCCEEEECCCCCEEEEEEEEECCCHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHH
INDYAKRFGLMKEADGSLSGDDVREGLTAIVSVKHPAPQFEGQTKTKLGNADARTATDTL
HHHHHHHCCCHHCCCCCCCCHHHHHHHHEEEECCCCCCCCCCCCHHCCCCCCCCHHHHHH
FSDTFEQFLLENPSVGRLIVDKGLMAARARLAAKRAREMTRRKGILEVSSLPGKLADCSS
HHHHHHHHHHCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCHHCCCC
KDASKSELYIVEGDSAGGSAKSGRDRHFQAILPIRGKIINVEKARLDKILANQEVRTIIT
CCCCCCEEEEEECCCCCCCCCCCCCCCEEEEECCCCEEEECHHHHHHHHHHCHHHHHHHH
ALGTGIADEFDLSKARYHKLIIMTDADVDGAHIRTLLLTFFYRYMRPLVESGYVYIAQPP
HHHCCCCCHHHCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEECCC
LYGVKHGKETIYVQNDRELQAAIAQLPENARYSVQRYKGLGEMDPEQLWNTTMNPETRSM
CCCCCCCCEEEEEECCHHHHHHHHHCCCCCHHHHHHHCCCCCCCHHHHHCCCCCCCHHHH
LRVDLQDAIEADEIFETLMGDNVEPRRDFIQSHAHYVKNLDI
HHHHHHHHCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10427704; 11058132 [H]