Click here to switch to the map view.

The map label for this gene is holB [H]

Identifier: 229917429

GI number: 229917429

Start: 1653245

End: 1654237

Strand: Reverse

Name: holB [H]

Synonym: EAT1b_1704

Alternate gene names: 229917429

Gene position: 1654237-1653245 (Counterclockwise)

Preceding gene: 229917430

Following gene: 229917428

Centisome position: 55.14

GC content: 45.22

Gene sequence:

>993_bases
ATGCGTTTTCAAGAATTGAGCGAGTCGCAAACTGTCGTTTCTCGCATCATTCAAAATTCAATCGTTTCGAATAAAGTGGG
GCATGCGTATATCTTCTCAGGAGATTATGAGCCTTATTTACTTGATACAGCCACATTGTTCGCTAAAGCCTTATTTTGTG
AATCACCAGATGGTGCAGAGCCTTGTGGAGTGTGTCGAAACTGTCGGCGCATGGATTCTAAAAATATCGTCGACTACATT
GAAGTGGAGCCGGACGGTGCGAGTATTAAAAAAGAACAGATCCAACAACTGCTCAGTGATTTATCACTACGAGGCGCTGA
AGGCGATCGCCAAGTGTATGTCATTCATCAAGCGCACAAGATGACGACTCAAGCGGCGAACAGTCTATTGAAGTTCTTTG
AAGAACCGGGTGTAGGCAAGCTTGCTATTTTAGTGACGACACAACCACAGCTGCTTCTTCCGACGATTCGTTCGCGTGCG
CAACAACTTGCTTTTCGTCCAATCGACCGAGCTCAGTTGGCGCAAACAATGTCAGAAGAACTGTCCTGTACGTATGATTT
GGCATACCTTGCCTTGACCGTTTATCCGAAATGGGATGACGCGAAAGAGGCACTTGAGCAGGAGTGGTTTGTACAGGCGT
ACGGGCTAGTGGTACAATTAATTGAAGTATTGACGAAACGACCTCAAGAGTTGCTCTTGTTCGCACAAGAAAAATGGTTA
GGGCATTTCAAAGGACGGAATGAGTTGCGAGTCGGTCTTGAATTATTCGAACATTGGTTAGAGGAGGCACTTCATTTAAA
AGTGCGAGATACGTATGCTCCGAAGCTATTTAAGAATGAGGCTGCCTTGTACAACGCGTTTATCCAAAATCGATCTGTCA
CCAAGCTTACACATGCGATTGAAAGTGTACATGCGGCCATTCGGCGACTTGAGGCAAACGTCAATCCTCAATTAACGTTC
GAACGACTTTCGTTCGATTTGCAGAAAGGATAG

Upstream 100 bases:

>100_bases
ATTCATATATTCCTTACCCGGTCGAAGTACAAGTCGGAGGGGCGACGGTATTCGTCTTGCCGATCGAAGGATTCCATCAG
TTTTAATGGGAGTTGGGCAT

Downstream 100 bases:

>100_bases
AAGCGCGTGCTTGAAGTAGTTGGAGTCCGTTTTAAAGAAGCGGGAAAAATCTATTACTTTTCACCAGGCGATGCAACGCT
TAAACGTGGGGACCATGTCA

Product: DNA-directed DNA polymerase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 330; Mature: 330

Protein sequence:

>330_residues
MRFQELSESQTVVSRIIQNSIVSNKVGHAYIFSGDYEPYLLDTATLFAKALFCESPDGAEPCGVCRNCRRMDSKNIVDYI
EVEPDGASIKKEQIQQLLSDLSLRGAEGDRQVYVIHQAHKMTTQAANSLLKFFEEPGVGKLAILVTTQPQLLLPTIRSRA
QQLAFRPIDRAQLAQTMSEELSCTYDLAYLALTVYPKWDDAKEALEQEWFVQAYGLVVQLIEVLTKRPQELLLFAQEKWL
GHFKGRNELRVGLELFEHWLEEALHLKVRDTYAPKLFKNEAALYNAFIQNRSVTKLTHAIESVHAAIRRLEANVNPQLTF
ERLSFDLQKG

Sequences:

>Translated_330_residues
MRFQELSESQTVVSRIIQNSIVSNKVGHAYIFSGDYEPYLLDTATLFAKALFCESPDGAEPCGVCRNCRRMDSKNIVDYI
EVEPDGASIKKEQIQQLLSDLSLRGAEGDRQVYVIHQAHKMTTQAANSLLKFFEEPGVGKLAILVTTQPQLLLPTIRSRA
QQLAFRPIDRAQLAQTMSEELSCTYDLAYLALTVYPKWDDAKEALEQEWFVQAYGLVVQLIEVLTKRPQELLLFAQEKWL
GHFKGRNELRVGLELFEHWLEEALHLKVRDTYAPKLFKNEAALYNAFIQNRSVTKLTHAIESVHAAIRRLEANVNPQLTF
ERLSFDLQKG
>Mature_330_residues
MRFQELSESQTVVSRIIQNSIVSNKVGHAYIFSGDYEPYLLDTATLFAKALFCESPDGAEPCGVCRNCRRMDSKNIVDYI
EVEPDGASIKKEQIQQLLSDLSLRGAEGDRQVYVIHQAHKMTTQAANSLLKFFEEPGVGKLAILVTTQPQLLLPTIRSRA
QQLAFRPIDRAQLAQTMSEELSCTYDLAYLALTVYPKWDDAKEALEQEWFVQAYGLVVQLIEVLTKRPQELLLFAQEKWL
GHFKGRNELRVGLELFEHWLEEALHLKVRDTYAPKLFKNEAALYNAFIQNRSVTKLTHAIESVHAAIRRLEANVNPQLTF
ERLSFDLQKG

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria [H]

COG id: COG0470

COG function: function code L; ATPase involved in DNA replication

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786676, Length=169, Percent_Identity=37.8698224852071, Blast_Score=102, Evalue=5e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004622 [H]

Pfam domain/function: NA

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 37647; Mature: 37647

Theoretical pI: Translated: 6.32; Mature: 6.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRFQELSESQTVVSRIIQNSIVSNKVGHAYIFSGDYEPYLLDTATLFAKALFCESPDGAE
CCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCC
PCGVCRNCRRMDSKNIVDYIEVEPDGASIKKEQIQQLLSDLSLRGAEGDRQVYVIHQAHK
HHHHHHHHHHCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHCCCCCCCCEEEEEEHHHH
MTTQAANSLLKFFEEPGVGKLAILVTTQPQLLLPTIRSRAQQLAFRPIDRAQLAQTMSEE
HHHHHHHHHHHHHHCCCCCEEEEEEECCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
LSCTYDLAYLALTVYPKWDDAKEALEQEWFVQAYGLVVQLIEVLTKRPQELLLFAQEKWL
HCHHHEEEEHHEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHH
GHFKGRNELRVGLELFEHWLEEALHLKVRDTYAPKLFKNEAALYNAFIQNRSVTKLTHAI
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCHHHHHHHH
ESVHAAIRRLEANVNPQLTFERLSFDLQKG
HHHHHHHHHHHCCCCCCEEHHHHHHHHCCC
>Mature Secondary Structure
MRFQELSESQTVVSRIIQNSIVSNKVGHAYIFSGDYEPYLLDTATLFAKALFCESPDGAE
CCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCC
PCGVCRNCRRMDSKNIVDYIEVEPDGASIKKEQIQQLLSDLSLRGAEGDRQVYVIHQAHK
HHHHHHHHHHCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHCCCCCCCCEEEEEEHHHH
MTTQAANSLLKFFEEPGVGKLAILVTTQPQLLLPTIRSRAQQLAFRPIDRAQLAQTMSEE
HHHHHHHHHHHHHHCCCCCEEEEEEECCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
LSCTYDLAYLALTVYPKWDDAKEALEQEWFVQAYGLVVQLIEVLTKRPQELLLFAQEKWL
HCHHHEEEEHHEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHH
GHFKGRNELRVGLELFEHWLEEALHLKVRDTYAPKLFKNEAALYNAFIQNRSVTKLTHAI
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCHHHHHHHH
ESVHAAIRRLEANVNPQLTFERLSFDLQKG
HHHHHHHHHHHCCCCCCEEHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7584024; 9384377 [H]