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The map label for this gene is cysK [H]

Identifier: 229917398

GI number: 229917398

Start: 1620463

End: 1621416

Strand: Reverse

Name: cysK [H]

Synonym: EAT1b_1673

Alternate gene names: 229917398

Gene position: 1621416-1620463 (Counterclockwise)

Preceding gene: 229917399

Following gene: 229917397

Centisome position: 54.05

GC content: 51.15

Gene sequence:

>954_bases
ATGCGTATCGTAAACTCAGTAACAGACTTGATTGGAAAGACTCCGATTGTGAAATTGAACGGAATGACTGGACCAGAGGA
TGCCAACGTGTATCTGAAGCTCGAATATATGAACCCGGGATCAAGCGTCAAAGACCGGATCGCCCTCGCCATGATCGAAG
CGGCCGAAAAAGACGGTATCTTGAAGCCAGGTGATACAATCATCGAGCCAACAAGCGGAAACACGGGCATCGGGCTCGCG
ATGGTCAGTGCCGCAAAAGGATATAAAGCCATTCTCGTCATGCCTGAAACGATGAGCATGGAACGCCGAAACCTCCTTCG
TGGATACGGGGCCGAATTGATTTTGACACCAGGACCTGAAGGGATGGGTGGAGCGATTCGTCGCGCTACTGAAGAAGCCG
AGAAACATGGCTACTTCCTCCCGCAACAATTCAATAACGCAGCCAACCCGAACGTTCATGAAATGACGACGGGACCTGAG
ATTGTGGAGGCGTTTGAAGAATCAGGACTTTCAGTCGACGCTTTGATTGCCGGAGTCGGGACAGGCGGTACCATTACTGG
AGCAGGTAAAGTCGTGAAGGCGAAGTACCCGGACGTAAAAATTCTCGCCGTTGAACCGGTCGATTCACCTGTCTTATCAG
GCGGGAAGCCGGGACCTCATAAGATTCAAGGGATTGGGGCTGGATTCGTTCCATCAATCCTCGACACGGAGATTTATGAA
GGGGTTCTTCAAATCACGACAGAGCAAGCGTTCGAATACGCTCGTCAGGCGGCACGTACGAACGGTGTCCTCGGTGGTAT
CTCTTCAGGAGCTGCGATTGCGGCAGCGCTTGATACAGCGAAACGTCTTGGTAAAGGGAAAAACGTATTGGCGATTATCC
CGTCAAACGGTGAACGTTACTTGAGCACACCGCTCTATCAGTTTGATGAAGAGTCAAACGAGAGTCGCGTATAA

Upstream 100 bases:

>100_bases
TCAGGCACAGGCGTAAGCAACGTTCCAAATTGCATATTTTCAATTCGATAGGGTATGATAACAATATCGGAATAGTCGGA
ATTATGAAGGAGGAAATGCA

Downstream 100 bases:

>100_bases
CATTTCAGGCAGTAATCGAGAGACATTCTCTTTCGATTGCTGCCTTTTCTTGTGTACAATAAAAGGCATGAATGAATAGA
CGGAGGAATGACATGTGAGA

Product: cysteine synthase A

Products: NA

Alternate protein names: CSase; O-acetylserine (thiol)-lyase; OAS-TL; Superoxide-inducible protein 11; SOI11 [H]

Number of amino acids: Translated: 317; Mature: 317

Protein sequence:

>317_residues
MRIVNSVTDLIGKTPIVKLNGMTGPEDANVYLKLEYMNPGSSVKDRIALAMIEAAEKDGILKPGDTIIEPTSGNTGIGLA
MVSAAKGYKAILVMPETMSMERRNLLRGYGAELILTPGPEGMGGAIRRATEEAEKHGYFLPQQFNNAANPNVHEMTTGPE
IVEAFEESGLSVDALIAGVGTGGTITGAGKVVKAKYPDVKILAVEPVDSPVLSGGKPGPHKIQGIGAGFVPSILDTEIYE
GVLQITTEQAFEYARQAARTNGVLGGISSGAAIAAALDTAKRLGKGKNVLAIIPSNGERYLSTPLYQFDEESNESRV

Sequences:

>Translated_317_residues
MRIVNSVTDLIGKTPIVKLNGMTGPEDANVYLKLEYMNPGSSVKDRIALAMIEAAEKDGILKPGDTIIEPTSGNTGIGLA
MVSAAKGYKAILVMPETMSMERRNLLRGYGAELILTPGPEGMGGAIRRATEEAEKHGYFLPQQFNNAANPNVHEMTTGPE
IVEAFEESGLSVDALIAGVGTGGTITGAGKVVKAKYPDVKILAVEPVDSPVLSGGKPGPHKIQGIGAGFVPSILDTEIYE
GVLQITTEQAFEYARQAARTNGVLGGISSGAAIAAALDTAKRLGKGKNVLAIIPSNGERYLSTPLYQFDEESNESRV
>Mature_317_residues
MRIVNSVTDLIGKTPIVKLNGMTGPEDANVYLKLEYMNPGSSVKDRIALAMIEAAEKDGILKPGDTIIEPTSGNTGIGLA
MVSAAKGYKAILVMPETMSMERRNLLRGYGAELILTPGPEGMGGAIRRATEEAEKHGYFLPQQFNNAANPNVHEMTTGPE
IVEAFEESGLSVDALIAGVGTGGTITGAGKVVKAKYPDVKILAVEPVDSPVLSGGKPGPHKIQGIGAGFVPSILDTEIYE
GVLQITTEQAFEYARQAARTNGVLGGISSGAAIAAALDTAKRLGKGKNVLAIIPSNGERYLSTPLYQFDEESNESRV

Specific function: Catalyzes the conversion of O-acetylserine to cysteine. Also acts as a sensor of cysteine availability in the signal transduction pathway modulating CymR activity. When cysteine is present, the pool of O-acetylserine (OAS) is low, which leads to the forma

COG id: COG0031

COG function: function code E; Cysteine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the cysteine synthase/cystathionine beta- synthase family [H]

Homologues:

Organism=Homo sapiens, GI295821202, Length=315, Percent_Identity=40, Blast_Score=221, Evalue=5e-58,
Organism=Homo sapiens, GI295821200, Length=315, Percent_Identity=40, Blast_Score=221, Evalue=5e-58,
Organism=Homo sapiens, GI4557415, Length=315, Percent_Identity=40, Blast_Score=221, Evalue=5e-58,
Organism=Homo sapiens, GI11345492, Length=208, Percent_Identity=29.3269230769231, Blast_Score=70, Evalue=2e-12,
Organism=Escherichia coli, GI1788754, Length=313, Percent_Identity=51.7571884984025, Blast_Score=264, Evalue=5e-72,
Organism=Escherichia coli, GI2367138, Length=304, Percent_Identity=42.4342105263158, Blast_Score=214, Evalue=5e-57,
Organism=Caenorhabditis elegans, GI115535073, Length=306, Percent_Identity=52.6143790849673, Blast_Score=271, Evalue=5e-73,
Organism=Caenorhabditis elegans, GI17535051, Length=302, Percent_Identity=48.3443708609272, Blast_Score=269, Evalue=1e-72,
Organism=Caenorhabditis elegans, GI17562970, Length=308, Percent_Identity=46.1038961038961, Blast_Score=256, Evalue=7e-69,
Organism=Caenorhabditis elegans, GI32566674, Length=303, Percent_Identity=46.2046204620462, Blast_Score=251, Evalue=3e-67,
Organism=Caenorhabditis elegans, GI17561720, Length=308, Percent_Identity=44.4805194805195, Blast_Score=223, Evalue=9e-59,
Organism=Caenorhabditis elegans, GI32566672, Length=216, Percent_Identity=44.9074074074074, Blast_Score=174, Evalue=5e-44,
Organism=Caenorhabditis elegans, GI17534315, Length=332, Percent_Identity=37.0481927710843, Blast_Score=172, Evalue=1e-43,
Organism=Caenorhabditis elegans, GI25147552, Length=331, Percent_Identity=36.2537764350453, Blast_Score=167, Evalue=8e-42,
Organism=Caenorhabditis elegans, GI17561716, Length=118, Percent_Identity=41.5254237288136, Blast_Score=92, Evalue=3e-19,
Organism=Saccharomyces cerevisiae, GI6321594, Length=320, Percent_Identity=37.8125, Blast_Score=195, Evalue=7e-51,
Organism=Saccharomyces cerevisiae, GI6321449, Length=340, Percent_Identity=35, Blast_Score=131, Evalue=2e-31,
Organism=Drosophila melanogaster, GI24643623, Length=314, Percent_Identity=38.2165605095541, Blast_Score=194, Evalue=5e-50,
Organism=Drosophila melanogaster, GI20129101, Length=314, Percent_Identity=38.2165605095541, Blast_Score=194, Evalue=5e-50,

Paralogues:

None

Copy number: 2820 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001216
- InterPro:   IPR005856
- InterPro:   IPR005859
- InterPro:   IPR001926 [H]

Pfam domain/function: PF00291 PALP [H]

EC number: =2.5.1.47 [H]

Molecular weight: Translated: 33313; Mature: 33313

Theoretical pI: Translated: 4.96; Mature: 4.96

Prosite motif: PS00901 CYS_SYNTHASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIVNSVTDLIGKTPIVKLNGMTGPEDANVYLKLEYMNPGSSVKDRIALAMIEAAEKDGI
CCHHHHHHHHHCCCCEEEECCCCCCCCCEEEEEEEEECCCCCHHHHHHHHHHHHHHHCCC
LKPGDTIIEPTSGNTGIGLAMVSAAKGYKAILVMPETMSMERRNLLRGYGAELILTPGPE
CCCCCCEEECCCCCCCCCEEEEECCCCCEEEEEECCHHHHHHHHHHHHCCCEEEECCCCC
GMGGAIRRATEEAEKHGYFLPQQFNNAANPNVHEMTTGPEIVEAFEESGLSVDALIAGVG
CCCHHHHHHHHHHHHCCCCCCHHHCCCCCCCCEEECCCHHHHHHHHHCCCEEEHEEEECC
TGGTITGAGKVVKAKYPDVKILAVEPVDSPVLSGGKPGPHKIQGIGAGFVPSILDTEIYE
CCCEEECCCEEEEEECCCEEEEEECCCCCCCCCCCCCCCCEEEECCCCCCHHHHHHHHHH
GVLQITTEQAFEYARQAARTNGVLGGISSGAAIAAALDTAKRLGKGKNVLAIIPSNGERY
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEECCCCCCE
LSTPLYQFDEESNESRV
ECCCHHHCCCCCCCCCC
>Mature Secondary Structure
MRIVNSVTDLIGKTPIVKLNGMTGPEDANVYLKLEYMNPGSSVKDRIALAMIEAAEKDGI
CCHHHHHHHHHCCCCEEEECCCCCCCCCEEEEEEEEECCCCCHHHHHHHHHHHHHHHCCC
LKPGDTIIEPTSGNTGIGLAMVSAAKGYKAILVMPETMSMERRNLLRGYGAELILTPGPE
CCCCCCEEECCCCCCCCCEEEEECCCCCEEEEEECCHHHHHHHHHHHHCCCEEEECCCCC
GMGGAIRRATEEAEKHGYFLPQQFNNAANPNVHEMTTGPEIVEAFEESGLSVDALIAGVG
CCCHHHHHHHHHHHHCCCCCCHHHCCCCCCCCEEECCCHHHHHHHHHCCCEEEHEEEECC
TGGTITGAGKVVKAKYPDVKILAVEPVDSPVLSGGKPGPHKIQGIGAGFVPSILDTEIYE
CCCEEECCCEEEEEECCCEEEEEECCCCCCCCCCCCCCCCEEEECCCCCCHHHHHHHHHH
GVLQITTEQAFEYARQAARTNGVLGGISSGAAIAAALDTAKRLGKGKNVLAIIPSNGERY
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEECCCCCCE
LSTPLYQFDEESNESRV
ECCCHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7584024; 9384377; 8755892; 9298659 [H]