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The map label for this gene is dus1 [H]

Identifier: 229917391

GI number: 229917391

Start: 1614948

End: 1615943

Strand: Reverse

Name: dus1 [H]

Synonym: EAT1b_1666

Alternate gene names: 229917391

Gene position: 1615943-1614948 (Counterclockwise)

Preceding gene: 229917392

Following gene: 229917390

Centisome position: 53.87

GC content: 51.61

Gene sequence:

>996_bases
TTGACAGGCTTTAAGATTGGAAACATAGAGATTAAAAACCGTGCAGTCCTCGCGCCGATGGCCGGCGTGACCAATCCCGC
TTTCCGTTTGATTGCAAAAGAGTTCGGAGCGGGACTCGTCTGTGCCGAAATGGTCAGTGATAAAGGCATCTTGCTCGAGA
ATGCTCGGACGATGCGCATGCTATACGTCGATGAACGAGAGAAACCGCTCAGTTTACAAATCTTCGGCGGTTCGAAAGAT
ACACTCGTCAGAGCGGCGCAGTACGTCGACCAAAACACCAACGCGGATATCATTGATATCAACATGGGTTGTCCCGTACC
GAAAGTGACGAAGTGTGACGCAGGGGCGAAGTGGTTACTCGACCCGGATAAAGTATATGAGATGGTGAATGCCGTCACGA
ATGTCGTCGAAAAACCGGTCACGGTGAAAATGCGCCTTGGTTGGGACGATGATCACATCTTTATTTTGGACAATGTGAAG
GCGGCTCAGACAGGTGGAGCGGCAGCCATTGCCATTCATGGACGTACGCGTGCCCAACAATATGAAGGAACCGCGAATTG
GGACTGGATTGGCGAGGCGAAGAAAGTCGCGACCGTACCGATTATAGGGAACGGGGACATTGCCACACCACAAGACGCAA
AGCGTGTCATTGACCAATATGGCGTGGATGCCGTCATGATCGGGCGCGCTGCACTTGGAAACCCGTGGATGCTCTATCAA
ACGGTCCAGTACCTCGAAACAGGGGAGCTCCCGCCGGAACCAGCAGCTCGCGAAAAAATTGATATTTGCATGTTGCACCT
CGACCGGTTGAGTGCAATCAAAGGTGAATTGACAGCCGTACGCGAAATGCGTCAACACGCGGCTTGGTATTTGAAAGGAT
TAAAAGGTAGTGGTCCGATTCGTAAGCAAATCAACGAAACGGAATCACGCGAAGCGTTCGCGGCGGTCCTTTATGGGTTT
GTGGACCAACTCGAACGACAACTGTCAGAAGTGTGA

Upstream 100 bases:

>100_bases
ACGGTCGCCTCTTGGCTTGAAGGGTTACCAATGACGGACCGCCAAGATGTCGTCCGGTTACAGTTGCTTACGAATTTGGT
GAAATGAAGGATGTGTTGTC

Downstream 100 bases:

>100_bases
ACTAGTGAAAAGAACTAATTTATGGTAAGCTTGTTTGGCGAAAAAGTGCACCACAACGAATGAACGGCTTCGGCTGTGCT
GTTGGCCATCGGGTCGACAG

Product: TIM-barrel protein, nifR3 family

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 331; Mature: 330

Protein sequence:

>331_residues
MTGFKIGNIEIKNRAVLAPMAGVTNPAFRLIAKEFGAGLVCAEMVSDKGILLENARTMRMLYVDEREKPLSLQIFGGSKD
TLVRAAQYVDQNTNADIIDINMGCPVPKVTKCDAGAKWLLDPDKVYEMVNAVTNVVEKPVTVKMRLGWDDDHIFILDNVK
AAQTGGAAAIAIHGRTRAQQYEGTANWDWIGEAKKVATVPIIGNGDIATPQDAKRVIDQYGVDAVMIGRAALGNPWMLYQ
TVQYLETGELPPEPAAREKIDICMLHLDRLSAIKGELTAVREMRQHAAWYLKGLKGSGPIRKQINETESREAFAAVLYGF
VDQLERQLSEV

Sequences:

>Translated_331_residues
MTGFKIGNIEIKNRAVLAPMAGVTNPAFRLIAKEFGAGLVCAEMVSDKGILLENARTMRMLYVDEREKPLSLQIFGGSKD
TLVRAAQYVDQNTNADIIDINMGCPVPKVTKCDAGAKWLLDPDKVYEMVNAVTNVVEKPVTVKMRLGWDDDHIFILDNVK
AAQTGGAAAIAIHGRTRAQQYEGTANWDWIGEAKKVATVPIIGNGDIATPQDAKRVIDQYGVDAVMIGRAALGNPWMLYQ
TVQYLETGELPPEPAAREKIDICMLHLDRLSAIKGELTAVREMRQHAAWYLKGLKGSGPIRKQINETESREAFAAVLYGF
VDQLERQLSEV
>Mature_330_residues
TGFKIGNIEIKNRAVLAPMAGVTNPAFRLIAKEFGAGLVCAEMVSDKGILLENARTMRMLYVDEREKPLSLQIFGGSKDT
LVRAAQYVDQNTNADIIDINMGCPVPKVTKCDAGAKWLLDPDKVYEMVNAVTNVVEKPVTVKMRLGWDDDHIFILDNVKA
AQTGGAAAIAIHGRTRAQQYEGTANWDWIGEAKKVATVPIIGNGDIATPQDAKRVIDQYGVDAVMIGRAALGNPWMLYQT
VQYLETGELPPEPAAREKIDICMLHLDRLSAIKGELTAVREMRQHAAWYLKGLKGSGPIRKQINETESREAFAAVLYGFV
DQLERQLSEV

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family [H]

Homologues:

Organism=Homo sapiens, GI239788483, Length=231, Percent_Identity=32.034632034632, Blast_Score=137, Evalue=1e-32,
Organism=Homo sapiens, GI239788462, Length=233, Percent_Identity=31.7596566523605, Blast_Score=136, Evalue=3e-32,
Organism=Homo sapiens, GI40807366, Length=325, Percent_Identity=25.8461538461538, Blast_Score=95, Evalue=1e-19,
Organism=Homo sapiens, GI31742496, Length=226, Percent_Identity=30.5309734513274, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI8923374, Length=246, Percent_Identity=29.6747967479675, Blast_Score=93, Evalue=3e-19,
Organism=Escherichia coli, GI1789660, Length=323, Percent_Identity=38.390092879257, Blast_Score=215, Evalue=3e-57,
Organism=Escherichia coli, GI1788462, Length=305, Percent_Identity=30.8196721311475, Blast_Score=103, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI17543114, Length=238, Percent_Identity=33.6134453781513, Blast_Score=129, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI17507177, Length=243, Percent_Identity=28.8065843621399, Blast_Score=100, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI17510279, Length=245, Percent_Identity=26.1224489795918, Blast_Score=86, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI25144369, Length=222, Percent_Identity=26.5765765765766, Blast_Score=79, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6323560, Length=231, Percent_Identity=29.004329004329, Blast_Score=94, Evalue=3e-20,
Organism=Saccharomyces cerevisiae, GI6323433, Length=284, Percent_Identity=25.7042253521127, Blast_Score=89, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6323437, Length=266, Percent_Identity=24.4360902255639, Blast_Score=73, Evalue=5e-14,
Organism=Drosophila melanogaster, GI24585320, Length=236, Percent_Identity=31.7796610169492, Blast_Score=131, Evalue=6e-31,
Organism=Drosophila melanogaster, GI24580595, Length=267, Percent_Identity=26.9662921348315, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI19920448, Length=267, Percent_Identity=26.9662921348315, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI45549423, Length=243, Percent_Identity=30.0411522633745, Blast_Score=89, Evalue=3e-18,
Organism=Drosophila melanogaster, GI19921524, Length=301, Percent_Identity=24.9169435215947, Blast_Score=77, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004652
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: NA

Molecular weight: Translated: 36457; Mature: 36325

Theoretical pI: Translated: 6.17; Mature: 6.17

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTGFKIGNIEIKNRAVLAPMAGVTNPAFRLIAKEFGAGLVCAEMVSDKGILLENARTMRM
CCCEEECCEEECCCEEEECCCCCCCHHHHHHHHHHCCCEEEHHHHCCCCEEEECCCEEEE
LYVDEREKPLSLQIFGGSKDTLVRAAQYVDQNTNADIIDINMGCPVPKVTKCDAGAKWLL
EEEECCCCCEEEEEECCCHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCEECCCCCEECC
DPDKVYEMVNAVTNVVEKPVTVKMRLGWDDDHIFILDNVKAAQTGGAAAIAIHGRTRAQQ
CHHHHHHHHHHHHHHHCCCEEEEEEECCCCCEEEEEECCCCCCCCCEEEEEEECCCHHHH
YEGTANWDWIGEAKKVATVPIIGNGDIATPQDAKRVIDQYGVDAVMIGRAALGNPWMLYQ
CCCCCCCCCCCCCCEEEEEEEECCCCCCCCHHHHHHHHHHCCCEEEECCHHCCCCCHHHH
TVQYLETGELPPEPAAREKIDICMLHLDRLSAIKGELTAVREMRQHAAWYLKGLKGSGPI
HHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCH
RKQINETESREAFAAVLYGFVDQLERQLSEV
HHHHCHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TGFKIGNIEIKNRAVLAPMAGVTNPAFRLIAKEFGAGLVCAEMVSDKGILLENARTMRM
CCEEECCEEECCCEEEECCCCCCCHHHHHHHHHHCCCEEEHHHHCCCCEEEECCCEEEE
LYVDEREKPLSLQIFGGSKDTLVRAAQYVDQNTNADIIDINMGCPVPKVTKCDAGAKWLL
EEEECCCCCEEEEEECCCHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCEECCCCCEECC
DPDKVYEMVNAVTNVVEKPVTVKMRLGWDDDHIFILDNVKAAQTGGAAAIAIHGRTRAQQ
CHHHHHHHHHHHHHHHCCCEEEEEEECCCCCEEEEEECCCCCCCCCEEEEEEECCCHHHH
YEGTANWDWIGEAKKVATVPIIGNGDIATPQDAKRVIDQYGVDAVMIGRAALGNPWMLYQ
CCCCCCCCCCCCCCEEEEEEEECCCCCCCCHHHHHHHHHHCCCEEEECCHHCCCCCHHHH
TVQYLETGELPPEPAAREKIDICMLHLDRLSAIKGELTAVREMRQHAAWYLKGLKGSGPI
HHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCH
RKQINETESREAFAAVLYGFVDQLERQLSEV
HHHHCHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7584024; 9384377 [H]